Insecticide treated mosquito bed nets, insecticide aerosol sprays, repellent lotions and creams, repellent candles, vaporizable repellent essential oils, mosquito mats and coils are some of the many malaria personal protection tools that are in use. The latter of these measures are the most accessible and affordable options for the rural population in sub-Saharan Africa. It is therefore important to determine how effective these personal protection measures are by determining their ability to efficiently disperse an active ingredient when the protection measure is in use. In this study, a copper II oxide montmorillonite clay burner (CuOMontmorillonite clay burner) was created to vaporize repellent formulations containing various concentrations of p-menthane-3,8-diol (PMD) as the active ingredient with the aim of determining the percentage of the active ingredient that is released into the air and the rate of release. The performance of the CuO-Montmorillonite clay burner was also compared to that of the Lampe Berger fragrance burner (LBFB). In addition to this, the percentage of PMD released from 6 percentage wt PMD candles of diameters 40mm, 69mm and 83mm was determined and compared to that released when the CuO-Montmorillonite clay burner and Lampe Berger Fragrance burner where used to vaporize PMD repellent formulations. It was found that the rate of vaporization of the various PMD repellent formulations vaporized using the Lampe Berger fragrance burner is affected by the mass of PMD present in the repellent formulations. The 6 percentage wt PMD repellent candles released the least percentage of PMD as compared to that released when the CuO-Montmorillonite clay burner and the Lampe Berger fragrance burner were used to vaporize the PMD repellent formulations.

The synthesis of para-menthane-3,8-diol in a batch reactor was investigated in some detail with the view to evaluate the potential of producing said p-menthane- 3,8-diol in a continuous-flow reactor from the results obtained from the batch process. The methodology used as base for this investigation was a published procedure by Takasago of Japan. The Takasago-method produced 92.3 percent Yield for the product para-menthane-3,8-diol, and 2.7 percent Yield of the by-product, acetal. The objective for this investigation was to produce a minimum p-menthane-3,8- diol content of 97.0 percent and a maximum content of 3.0 percent for the by-product acetal. The batch production process was evaluated in detail using statistical experimental design methodologies. Three process variables, namely catalyst loading, organic/aqueous phase ratio and reaction temperature were selected for the study. The experimental method was based on the Takasago procedure, however the substrate was added as a single slug as opposed to gradual addition method and the reaction period was reduced to 30 minutes. Apart from statistical analysis, mechanistic aspects were also used to interpret the following results. Using a central composite design, three response models (one for the conversion of citronellal, p-menthane-3,8-diol and acetal formation) were determined. An analysis of the response surfaces indicated that, to increase the citronellal conversion all three variables should be increased. To increase the amount of pmenthane- 3,8-diol, the reaction temperature and acid concentration should be increased, but the Aq/org ratio should be decreased as the acid catalyst concentration is increased. To minimize the amount of acetals formed during the reaction, the Aq/org ratio should be decreased; temperature and acid concentration can be decreased or increased. The reaction mechanism suggested that p-menthane-3,8-diol may be formed along two pathways: One pathway directly forms p-menthane-3,8-diol, whilst the second pathway forms the isopulegol first, then proceeds to form product by hydrolysis. The acetal is formed as result of the reaction between unreacted citronellal and p-menthane-3,8-diol. From the design experiments it was suggested that reaction time can be reduced to 8 minutes at reaction temperatures between, 80-85 0C The product and acetal were isolated by simple vacuum evaporation of the low boiling citronellal and isopulegol. Results from recycling the catalyst phase were similar to those of the initial process. The results of this investigation has clearly shown that with a proper understanding of the effect of process variables on the performance of the batch synthesis route, the conversion of this traditionally batch (actually semi-batch) process into a continuous process is quite feasible provided that suitable equipment is available. The most important features required for such equipment would be: Intense mixing throughout the reaction zone so as to maximise the surface area between the two immiscible phases, hence the rate of mass transfer between the two phases; and the ability to run reactions above the boiling point of water. Plans for the further study of the process are already well underway and sections of static mixing tubes have been acquired to build a continuous lab scale tubular reactor that would be capable of providing the level of mixing required.

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A model for the repellent effect of pyrethroid insecticides on insects was developed. Experiments were conducted using a small colony of honey bees in a flight cage. Conditioning to scented feeders allowed the separation of foraging bees from a single colony into treatment and control groups. Permethrin, cypermethrin, fenvalerate and flucythrinate were shown to be contact repellents to honey bees; exposure was primarily to the tarsi and ventral abdomen. The threshold dose of permethrin required to induce repellency was ca. 3.8 ng/bee. Repellency was fully reversible within 24 hours. No permanent effects on either memory or foraging efficiency were observed following acute exposure.

Made available in DSpace on 2014-06-11T19:35:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-20Bitstream added on 2014-06-13T19:25:15Z : No. of bitstreams: 1 tofolo_vc_dr_rcla_prot.pdf: 19466486 bytes, checksum: bad26d57e8eb11a239b119205310d9c6 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Ectatomma opaciventre (Hymenoptera: Formicidae: Ectatomminae) apresenta características morfológicas e comportamentais plesiomórficas, como a presença de um ferrão funcional, monoginia, colônias pequenas e estratégia de forrageamento solitário. Vive tanto nos ambientes preservados quanto nos alterados pelo homem, como os agroecossistemas, estando constantemente em contato com os produtos destinados ao controle de formigas consideradas pragas, como as cortadeiras do gênero Atta. Pouco se conhece a respeito de sua biologia básica (como dieta, rotas de forrageamento, distribuição espacial dos ninhos, composição dos hidrocarbonetos cuticulares) e das conseqüências advindas de seu contato com as iscas formicidas usadas para o controle de formigas cortadeiras em pastagens. Dessa forma, foi determinado por meio do “Método do Vizinho Mais Próximo” o padrão de distribuição espacial de seus ninhos em uma área degradada de 1800m2 na cidade de Rio Claro/SP/Brasil. Como na maioria das espécies de formigas tropicais, os ninhos de E. opaciventre apresentaram distribuição espacial do tipo regular ou hiperdispersa, com densidade de 0,018 ninho/m2. A distância média encontrada para o vizinho mais próximo foi de 5,57m, variando de 1,93m a 12,96m. Em vista da dificuldade em encontrar a rainha desta espécie, foi utilizado o método da “Espectroscopia Fotoacústica no Infravermelho com Transformada de Fourier (FTIR)” para identificar os hidrocarbonetos cuticulares da única rainha encontrada e das operárias de 9 ninhos satélites. Também foi determinado o grau de similaridade entre elas. Com os resultados obtidos ficou evidenciada a condição polidômica nessa espécie devido à semelhança entre o perfil químico cuticular da rainha e das operárias de ninhos vizinhos. Além disso... / Ectatomma opaciventre belongs to the subfamily Ectatomminae, which presents morphological and behavioral characteristics typical of more basal groups, such as the presence of a functional sting, monogyny, small colonies, and individual foraging strategies while searching for food. This species lives both in preserved and modified environments, such as agroecosystems, and it is constantly in contact with products intended to control ants regarded as pests, such as leaf-cutting ants of the genus Atta. The patterns of spatial distribution of nests over a 1800m2 degraded area in the city of Rio Claro, SP, Brazil were determined using a nearest neighbor method. As in most tropical species, the nests of E. opaciventre presented a regular or hyperdispersed spatial distribution, with a density of 0.018 nests/m2. The average nearest neighbor distance was 5.57 m, ranging from 1.93 m to 12.96 m. Considering the difficulty in finding queens of this species, the Fourier Transform-Infrared Photoacoustic Spectroscopy (FTIR-PAS) was used to identify cuticular hydrocarbons of the only queen found and of workers from nine satellite nests. The degree of similarity between them was also determined. From the results, polydomy was evident in this species because of the similarity between the cuticular chemical profile of the queen and that of workers from neighboring nests. Moreover, it was observed that the colonies that were not collected disappeared one year after the removal of the queen from the study area. In another area in the city of Rio Claro, of 4,800 m2, the diet of this species was studied by identifying the prey collected during the period of highest foraging activity. Besides, the foraging routes of three nests were determined by following foragers during their excursions in the field. The maximum foraging distance was... (Complete abstract click electronic access below)

The present product development project was aimed at studying the synergism and/or antagonism amongst various known insect repellent actives with the view to formulating a multi-active repellent product with improved properties when compared to current single-active commercial products. Advanced statistical methods were used to identify synergism between individual active substances and to define a formulation as close as possible to the “ideal” formulation. Several mosquito repellent samples were prepared and sent to the South African Bureau of Standards (SABS) in Pretoria to test for their efficiency in repelling mosquitoes. From the results of the repellency tests of the various active combinations, three actives were identified that showed promising signs of synergism. These actives were then studied in further detail to determine their optimum combination. In addition, it was shown that when using a natural flavourant as promoter and incorporating a slow-release agent into formulations for aerosols and lotions, a product is obtained that gives comparable levels of efficiency to current commercial products, but at much reduced levels of active loading. Accelerated stability tests performed on the final combination of the three actives used in the final formulation showed no adverse reactions over a three-week study. These tests shall be repeated once the final application form (lotion, aerosol, etc) and product packing have been decided.

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Call number: LD2668 .T4 1955 B49 / Master of Science

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