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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Tratamento de mudas de bananeira Prata Anã com o fungo Pochonia chlamydosporia visando proteção contra o nematoide das galhas Meloidogyne javanica / Treatment of banana seedlings Prata Anã with the fungus Pochonia chlamydosporia aiming protection against the root-knot nematode Meloidogyne javanica

Barbosa, Rosana Toledo 23 February 2017 (has links)
Submitted by Marco Antônio de Ramos Chagas (mchagas@ufv.br) on 2018-01-24T12:14:18Z No. of bitstreams: 1 texto completo.pdf: 779866 bytes, checksum: 232c3693dbb394fcf079f5d50f5c175a (MD5) / Made available in DSpace on 2018-01-24T12:14:18Z (GMT). No. of bitstreams: 1 texto completo.pdf: 779866 bytes, checksum: 232c3693dbb394fcf079f5d50f5c175a (MD5) Previous issue date: 2017-02-23 / Fundação de Amparo à Pesquisa do Estado de Minas Gerais / A micropropagação de mudas de bananeira vem se tornando cada vez mais comum devido ao ganho em escala e tempo na produção, além de se evitar a disseminação de patógenos, que ocorre frequentemente na propagação natural da cultura. Além disso, as mudas micropropagadas podem ser tratadas com microrganismos benéficos, que conferem proteção e vigor extras, agregando valor ao produto. O fungo Pochonia chlamydosporia já é conhecido por sua capacidade de controlar nematoides fitoparasitas e por promover o crescimento vegetal. Sendo assim, este trabalho foram avaliados os benefícios que esse fungo endofítico pode agregar ao ser introduzido em mudas micropropagadas de bananeira durante o período de aclimatação, antes de serem expostas ao contato com o nematoide das galhas radiculares, Meloidogyne javanica. Doses do produto Rizotec®, à base do isolado Pc-10 do fungo P. chlamydosporia, a fim de gerarem concentrações de 2.000, 3.000, 4.000 e 5.000 clamidósporos.g -1 de substrato, foram comparadas quanto à capacidade de conferir proteção e promover crescimento às mudas de banana. As diferentes concentrações do fungo resultaram em redução do número de galhas e de ovos de M. javanica por grama de raiz. O número de galhas e de ovos de M. javanica.g -1 de raízes, na concentração de 5.000 clamidósporos.g -1 de substrato, foram reduzidos em até 47,6% e 67%, respectivamente, em relação ao tratamento controle. Para as variáveis de desenvolvimento da planta, a altura da parte aérea, massa da parte aérea seca, diâmetro do pseudocaule e massa fresca das raízes de bananeira houve maior incremento na concentração de 5.000 clamidósporos.g -1 de substrato quando comparado ao controle. O fungo foi detectado colonizando as raízes das plantas e o substrato ao final do experimento, mostrando sua capacidade de se instalar, multiplicar e sobreviver no ambiente da muda micropropagada, e foi eficiente na redução dos danos causados pelo nematoide e na promoção do crescimento das plântulas de bananeira. / Micropropagation of banana seedlings has become increasingly common due to the gain in scale and time in the production, as well as avoiding the spread of pathogens, which occurs frequently in the natural propagation of the crop. In addition, micropropagated seedlings may be treated with beneficial microorganisms, which provide extra protection and vigor, adding value to the product. The fungus Pochonia chlamydosporia is already known for its ability to control plant-parasitic nematodes and to promote plant growth. Thus, this work evaluated the benefits that this endophytic fungus can add if introduced in micropropagated seedlings during the acclimatization period, before they are exposed to contact with the root gall nematode, Meloidogyne javanica. Doses of the Rizotec® product, based on the P. chlamydosporia fungus Pc-10 isolate, to generate 2000, 3000, 4000 and 5000 chlamydospore.g -1 concentrations in the substrate were compared for their ability to confer protection and promote the growth of banana seedlings. The different concentrations of the fungus resulted in a reduction in the number of galls and eggs of M. javanica per gram of root. The number of root galls showed a greater reduction in the concentration of 5,000 chlamydospores.g -1 than in the other treatments, with a reduction of up to 47.6% when compared to the control treatment. The number of eggs of M. javanica.g -1 of roots obtained a reduction of 67% at the concentration of 5,000 chlamydospores.g -1 of substrate, in relation to the control treatment. The dry shoot mass increased by up to 23% to 5,000 chlamydospores.g - soil. The diameter of the pseudostem also increased with the fungus application, showing an increase of 14%. The fresh root mass of the plants was also higher in the treatments with the fungus, with a 11.4% increase in the concentration of 5,000 chlamydospores.g -1 , in relation to the control treatment. The fungus was detected colonizing the roots of the plants and the substrate at the end of the experiment, showing its ability to install, multiply and survive in the micropropagated seedling environment, and was efficient in reducing the nematode damage and in promoting banana seedling growth.

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