The plants and animals that inhabit river channels may act as zoogeomorphic agents affecting the nature and rates of sediment recruitment, transport and deposition. The impact of benthic-feeding fish, which disturb bed material sediments during their search for food, has received little attention, even though benthic feeding species are widespread in rivers and may collectively expend significant amounts of energy foraging across the bed. A series of experiments were conducted to investigate the impacts of benthic feeding fish on the structure and composition of gravel-bed river sediments, and the implications for bed material transport. An ex-situ experiment was conducted to investigate the impact of a benthic feeding fish (European Barbel Barbus barbus) on particle displacements, bed sediment structures, gravel entrainment and transport fluxes. In a laboratory flume, changes in bed surface topography were measured and grain displacements examined when an imbricated, water-worked bed of 5.6-16 mm gravels was exposed to feeding juvenile Barbel. For substrates that had been exposed to feeding fish and control substrates which had not, grain entrainment rates and bedload fluxes were measured under a moderate transport regime. On average, approximately 37% of the substrate, by area, was modified by foraging fish during a four-hour treatment period, resulting in increased microtopographic roughness and reduced particle imbrication. Structural changes caused by fish increased bed load flux by 60% under entrainment flows, whilst on average the total number of grains transported during the entrainment phase was 82% higher from substrates that had been disturbed by Barbel. An ex-situ experiment utilising Barbel and Chub Leuciscus cephalus extended this initial study by considering the role of fish size and species as controls of sediment disturbance by foraging. Increasing the size of Barbel had a significant effect on measured disturbance and bedload transport. Specifically, the area of disturbed substrate, foraging depth, microtopographic roughness and sediment structure all increased as functions of fish size, as did bedload flux and total transported mass. In a comparison of the foraging effects of like-sized Barbel and Chub 8-10 in length, Barbel foraged a larger area of the riverbed and had a greater impact on microtopographic roughness and sediment structure. Foraging by both species was associated with increased sediment transport, but the bed load flux after foraging by Barbel was 150% higher than that following foraging by Chub and the total transported mass of sediment was 98% greater. An in-situ experiment quantified the effects of foraging fish, primarily Cyprinids (specifically Barbel and Chub), on gravel-river bed sediment structures, surface grain-size distributions, sediment transport fluxes and grain entrainment in the River Idle, Nottinghamshire, UK. This was achieved by installing large experimental sediment trays seeded with food at typical densities. The experiments yielded data about 1) topographic and structural differences between pre- and post-feeding substrates using DEMs interpolated from laser scans, 2) modifications to surface and sub-surface grain-size distributions as a function of fish foraging and 3) differences in sediment entrainment from water-worked substrates exposed to feeding fish and control substrates, without fish. Small sections of the substrate trays were recovered in tact from the field and for substrates that had been exposed to feeding fish and control substrates which had not, grain entrainment rates and bedload fluxes were measured under a moderate transport regime in the laboratory. On average, approximately 74% of the substrate, by area, was modified by foraging fish during a twelve-hour period, resulting in increased microtopographic roughness and substrate coarsening which had significant implications for bed material transport during the steady entrainment flow. Together, results from these experiments indicate that by increasing surface microtopography, modifying the composition of fluvial substrates and undoing the naturally stable structures produced by water working, foraging can influence sediment transport dynamics, predominately by increasing the mobility of river bed materials. The implication of this result is that by influencing the quantity of available, transportable sediment and entrainment thresholds, benthic feeding may affect sediment transport fluxes in gravel-bed rivers. In addition, three discrete studies were performed alongside the core experiments described above. A quantitative examination of habitat conditions favoured by feeding Barbel was conducted in the River Idle (Nottinghamshire, UK) which served to supplement existing literature pertaining to Barbel ecology, and inform experimental design during the core experiments. Two further studies considered the potential importance of foraging as a zoogeomorphic activity in terms of spatial extent, at a variety of scales, thereby extending core experiments to larger spatial scales in-situ.

In the present study, the effect of different temperature was tested on the growth and survival of yearlings in common barbel (Barbus barbus L.). This study discribes two studies wich we made under controlled conditions in fish breeding object VÚHR JU Vodňany and fish breeding object Tisová.

M.Sc. Zoology / Only a few species of the family Diplozoidae have previously been described from Africa, from various Labeo and Barbus species. An investigation was undertaken respectively in the Vaal Dam and Vaal River Barrage in the Vaal River system, South Africa to determine aspects of the morphology, taxonomy and ecology of specimens of this family collected from the gills of Barbus aeneus. Various fish species, namely B. aeneus, Barbus kimberleyensis, Labeo capensis, Labeo umbratus, Cyprinus carpio, Clarias gariepinus and Micropterus salmoides, were collected with the aid of gill nets. The fish were killed and the length, weight and sex determined. The gills were removed from the left and right gill chambers, placed in separate, marked petri dishes and covered with water from the dam. Gills (from first to fourth pair) were examined with the aid of a dissection microscope. The position of attachment and the region on the gill namely dorsal, median or ventral, was noted. Parasites were removed, fixed between a cover slip and glass slide in steaming hot aceto-formaldehyde alcohol and preserved in 70 % ethanol. A morphological study was undertaken. Results of a Scanning Electron Microscopy study illustrated the following external characteristics namely a round opisthohaptor with four pairs of clamps, while study of whole mounts revealed larval hooks, a round-ended intestine and eggs without filaments. Graphic reconstruction of serial sections of the reproductive system of parasites embedded in resin revealed that the vas deferens of one individual opens into the common vitelline duct of the other. Parasites were identified as belonging to the genus Paradiplozoon. The morphological characteristics, namely the round opisthohaptor, intestine with rounded ending, egg without filament and morphological measurements, of various structures (for example, total body length) that differ from other species of this genus, led to the establishment of a new species, that is, P. alwinii.

M.Sc. (Zoology) / The catchment area of the Olifants River is being subjected to increased agricultural and mining activities, industrial development and urbanisation. As a result of this, the water quality 'of the Olifants River and some of its tributaries (e.g. Selati River) has been deteriorating since 1983. This causes reason for concern as one of the downstream water users in the Olifants River catchment is the Kruger National Park, which requires of good quality to sustain its terrestrial and aquatic ecosystems It was therefore necessary to determine what extent activity midstream-or--tlie I Olifants River, especial y in the Phalaborwa area, influenced the water quality of the Olifants River. In this study, an evaluation was done of the water quality (physically and chemically of the Lower Olifants River inside of the Kruger National Park, as well as the Lower Selati River, a tributary of the Olifants River which flows through the Phalaborwa area. Special attention was paid to the metal concentrations in the water, sediment and fish. Water and sediment were sampled every alternate month from April 1990 to February 1992 at six sampling sites along the Lower Olifants River and one in the Selati River. Standard methods were used to determine the physical and chemical characteristics (e.g. pH, TDS, etc.) of the water. The fish species Barbus marequensis was also sampled every alternative month, but only at three sampling sites along the Olifants River and one in the Selati River. Organs and tissues that were dissected, included the gills, fat, liver, gonads, gut, muscle, skin, blood, vertebrae, kidney and bile, as well as the gut contents. In February 1992, additional sampling was performed at Pioneer Dam, a natural reference point used in this study. Atomic absorption spectrophotometry was used in the laboratory to determine the Cr, Cu, Fe, Mn, Ni, Pb, Sr and Zn concentrations in the water, sediment and fish samples. (The water quality of the Selati River was found to be stressful to aquatic life, especially .) with regard to the sodium, fluoride, chloride, sulphate, potassium, total ~dissolved salts 1 and metal concentrations (except strontium). The Selati River also influenced the ': water quality of the Olifants River after the Selati-Olifants confluence. Most of the :. time the water quality of the Lower Olifants River in the KNP did, however, comply With the recommended guideline limits, except for the metal concentrations. The high metal concentrations detected in the water (in some cases sub-lethal levels) indicated some degree of metal pollution, but, due to the hardness of the Olifants River water, conditions were not necessarily toxic to the aquatic life. The accumulated metals in the organs and tissues of marequensis gave a good indication of the metal levels to which the fish were exposed. More metals were taken up by the fish from April 1990 to February 1991 than from April 1991 to February 1992. The various metals were . distributed differently to the tissues of B. marequensis, with the highest zinc concentrations being accumulated by the skin and ovaries; the highest copper and iron by the liver, kidney and gut; the highest chromium and nickel by the blood; and the highest manganese, lead and strontium by the vertebrae and gills. These results afforded guidelines as to the types of tissue which should be sampled as the most suitable for the analysis of the various metals in polluted waters.

A large sportfishery that targets both smallmouth (Labeobarbus aeneus) and largemouth (Labeobarbus kimberleyensis) yellowfish exists in South Africa. Both species have high conservation priority, and no assessments documenting the effect of angling on L. aeneus and L. kimberleyensis have been undertaken. The overall aim of this study was to provide an assessment of the impact of angling on L. aeneus and L. kimberleyensis. The specific objectives of this study were to characterise the sectors utilising Lake Gariep, document catch, effort and total catch for the fishery as well as the locality specific biology of L. aeneus and L. kimberleyensis. The study was undertaken on Lake Gariep, South Africa's largest impoundment, situated on the Orange River system in central South Africa. Subsistence fishers were the dominant user group, constituting 60 % of the fishery, the remainder constituted recreational anglers. Angler catches were dominated by carp (Cyprinus carpio; 74 %), followed by mudfish (Labeo capensis; 13 %) and smallmouth yellowfish (8 %). Catches of largemouth yellowfish contributed < 0.5 % to the total catch. The relative abundance of species by weight differed by area (χ² test of independence: χ² = 182, df = 4, p ≤ 0.05). On any sampling day time fished was the best predictor for differences in probability of capture (PC) (Wald X²(1) = 7.169, p = 0.007). The probability of capturing L. aeneus differed significantly between month (Wald X²(5) = 20.690, p = 0.000) and region (Wald X²(3) = 46.755, p = 0.000). The single best predictor of differences in log abundance and non-zero CPUE was region (Factorial ANOVA p ≤ 0.05). Mean CPUE ranged from 0.21 ± 0.06 kg. man⁻¹.hr⁻¹ to 0.82 ± 0.11 kg. man⁻¹.hr⁻¹ in the OV region and 0.42 ± 0.10 kg. man⁻¹.hr⁻¹ to 1.17 ± 0.24 kg. man⁻¹.hr⁻¹ in the GD region. Angler effort was higher in OV than in GD and ranged from 17 ± 3 anglers/day to 45 ± 9 anglers/day and 6 ± 1 anglers/day to 41 ± 8 anglers/day, respectively. Total catch was higher in the GD 46.0 [95 % CI = 15:102.6] t. period⁻¹ than the OV region 40.0 [95 % CI = 13.9:89.6] t. period⁻¹. The total catch from the Lake Gariep fishery between March and December 2007 was estimated to be 86.0 [95 % CI = 40.4:154.8] t. period⁻¹. Age and growth was determined using whole otoliths. The growth of L. aeneus was best described by the von Bertalanffy growth model as Lt = 481.80 (1- e⁻°·²²⁽t⁺°·⁶¹⁾). Gonadal development for L. aeneus was seasonal, with the gonadosomatic index peaking in January, revealing a distinct spawning season. The length at 50 % maturity for female L. aeneus was attained at a fork length of 354.7 mm. Natural mortality (M) was estimated at 0.55 year⁻¹. The growth of L. kimberleyensis was described by the von Bertalanffy growth model as Lt = 763.22 (1- e⁻°·¹¹⁽t⁺°·⁶³⁾). Only 6 mature female and 15 mature male L. kimberleyensis were recorded during the study period. The smallest mature female was a 390 mm FL stage four female and the earliest recorded mature male was a 337 mm FL, ripe running male. Natural mortality (M) was estimated at 0.08 year⁻¹ for L. kimberleyensis. Per recruit analysis indicated that current fishing mortality reduces the L. aeneus spawner biomass by 7 %, which is considered negligible. Labeobarbus kimberleyensis forms an insignificant proportion of anglers catches and stock status is currently considered pristine.

This thesis explores the biology, ecology, and life-history styles of two closely-related redfin minnows, Pseudobarbus afer and P. asper (pisces; Cyprinidae), which both occur in the Gamtoos River system of South Africa. Five of the seven species of flexible-rayed redfin minnows are in the South African Red Data Book - Fishes. This investigation was designed to provide the data which would enable conservation authorities to manage the remaining populations of the Pseudobarbus species. A thorough understanding of the Gamtoos River system was necessary to properly interpret the findings of this study. The palaeo river systems and the changing climates since the break-up of Gondwanaland are discussed so that the present day environments could be considered as well as the past environmental changes. P. afer and P. asper occur in the Gamtoos River system with no physical barrier separating the two species. P. afer only occurs in the clear mountain streams of the Cape Fold Mountain Belt whereas P. asper occurs in the highly saline and turbid Karoo section of the system. P. afer were found to be the more precocial form of the sister species. They had bigger eggs, lower relative fecundity, shorter breeding season, lower gonadosomatic indices, larger first feeding larval fish, matured later and had a longer life-span than did P. asper, which had more altricial life-history attributes. They differ in their tradeoffs with P. asper devoting more resources earlier to reproduction and having a shorter lifespan. The improvement in the one aspect of fitness (early maturity) leads to the deterioration in another, namely lifespan. Both species undertake breeding migrations to riffle areas where they spawn in mid-channel immediately above a pool after an increase in water flow. P. afer and P. asper are non-guarders of their non-adhesive eggs and young, open substrate spawners on coarse substrates (rocks) and have photophobic free embryos. The breeding season is shorter for P. afer whereas P. asper can spawn as late as April and impoundment releases can induce them to spawn. A study of comparative neuroecology revealed that of the four groups of fish analyzed (males and females of both species) male P. afer had the largest brains, especially the optic lobes and cerebellum. P. asper females had the smallest brains. No neural compensation in the external gustatory centre, the facial lobe, was found for P. asper inhabiting the turbid waters. P. afer also had significantly larger eyes and longer barbels. P. afer males were also found to have the highest density and largest nuptial tubercles as well as the most pronounced breeding colouration. It was concluded that P. asper is the more derived of the sister species pair with regard to life-history attributes. It is further suggested that investment per offspring is important in determining the life-history trajectories. Paedomorphosis has occurred and by this mechanism variability has been restored to the redfin minnows in the Groot River which enables them to survive in the highly variable, intermittent Karoo stream. The more precocial P. afer do not require this variability in the more constant and predictable environment of the Wit River.

Barbel (Barbus barbus L.) is autochthonous species with batch-spawning, incident in the whole territory in the Czech republic. Number of this species was decrease markedly at last thirty years ago. Continual fish production is possible obtained to creation breeding under controlled conditions. Aim of thesis was detect basic characteristics of reproduktion under controlled conditions in fish breeding object VÚHR JU Vodňany. Fertilization, hatching performance was detect and quality offspring by osmotic test subsequently. These realized valuables about domesticated barbel were compared with same valuables hormonal induce ovulation adult fish and with valuables in spawning barbel directly in place of catching. The best results fertilization, hatching performance and resistance of larvae were reached in barbel spawning after cathing fish in the river Doubrava (Čáslavsko). After that follows group fish treated by hormonal preparation in the hatchery in Třebíč. Domesticated barbel spawning from January to June in periodic intervals, but their characteristics of reproduction are the worst demonstrably.

Thesis (Ph. D. (Zoology)) -- University of Limpopo, 2009. / Aspects of the ecology and biology of the Lowveld largescale yellowfish (Labeobarbus marequensis) were studied in the Luvuvhu River over a period of three years. In this study the origin of the species, its phylogenetic relation to the other South African yellowfish species, its distribution and gross morphology are discussed and the lack of knowledge regarding aspects of the species is pointed out. The study area is identified and its geology, hydrology, climate and water quality as well as the historic distribution of the species is discussed. Adapted “truss” techniques were applied to measure and calculate the morphometric features related to feeding and habitat preference of the species. From this data the habitat preferences and requirements were inferred after which it was compared to data obtained during field surveys. The study of the breeding biology and ecology of the species included investigations of gonad and egg structure and development as well as seasonal surveys of selected breeding sites. The diet of the species was established through stomach content analyses and related to the digestive tract morphology. Data obtained from the Xikundu fishway was used to establish the migratory behaviour. Results show that nine distinct stanzas or growth phases, each with its own morphometric characteristics, were identified. The body form, and some morphological aspects, of the species make it suitable to cope with flowing water. Ontogenetic changes in body form and the identified morphological aspects were observed and related to the habitat preferences of the stanzas. A distinct ontogenetic shift in preferred habitat was illustrated. The species was shown to be fractional spawner with two spawning events per annum. A major extended spawning event occurred during spring or early summer and coincided with a temperature increase and in particular with an increase in flow. Breeding occurred at sites with fast flowing water over cobble or boulder beds and it was observed that the presence of nursery areas related to breeding biotopes was extremely important. Although the diet of the species was dominated by plant and algal matter, juvenile stanzas ingested large amounts of animal material. It was found that the spatial movements of the species could be characterised as migrations and that breeding and dispersal migrations occurred.

M.Sc. (Zoology) / An investigation was made into the ecology of Barbus brevipinnis and Opsaridium zambezense in the Incomati River System in the Eastern Transvaal. Special attention was given to factors which may threaten the existence of both species. New information was collected on aspects concerning their length:mass relationship, condition, fecundity, feeding habits as weIl as parasites. Measurements were made of the standard, fork and total lengths of both species, which in each case was related to the specific mass of the fish. Both species are, according to the information obtained, capable of successive spawning over an extended period during the breeding season. This implies that the evaluation for fecundity according to standard procedures may in both species be conservative in terms of estimates of the total numbers of eggs produced during the breeding season. Indications are, however, that of Zambezense may produce relatively low numbers of eggs and this as weIl as certain environmental factors, may in combination explain the relative scarcity of this fish in the Inkomati River System. Environmental pollution may further complicate this issue. One of the main objectives of this study was to investigate the habitat requirements of both species. In doing so, both species can now be better defined in terms of certain biological requirements as welI as environmental conditions under which they occur. It is hoped that this information may contribute towards a better understanding of the environmental conservation status of both species. The scarcity collection of studies into including GSI. of both species prohibited the large scale specimens normally required to enable intensive factors such as feeding, growth and breeding, It was therefore not possible during this study to obtain an accurate assessment of parameters such as fecundity, which should be investigated in more detail if and when material on both species becomes available. Recommendations are made concerning morphometric and electrophoretic investigations of both species in order to evaluate their species status more accurately. It is suggested that the habitats where B.brevipinnis and O.zambezense occur be closely monitored in future to observe any further deterioration which may be caused by agricultural and mining activities. It is recommended that because of the relative scarcity of Zambezense in the Inkomati River System, and to the apparent sensitivity of this species to environmental disturbance of its habitat, its conservation status be changed from indeterminate scarce to vulnerable.

Ph.D. (Zoology) / Please refer to full text to view abstract