Colonisation theory relative to introduced populations was examined with a view to determining the usefulness of theoretical concepts in the prediction of the success of an invasion by a species. To this end, the Great Fish River, with reference to species introduced by the Orange/Fish Tunnel, was used as a case history. It was concluded that theoretical descriptions of population growth, control and decline are useful only when considering individual ecosystems or species. The highly variable nature of species and environments nullifies attempts to simplify behavioural characteristics into a predictive framework. There is an abundance of terminology associated with introduced organisms and frequently many of these terms are used synonymously. Eleven terms have been defined in this thesis with a view to standardising terminology. Characteristics of species and environments which enhance their susceptibility to invasions by exotic ichthyofauna were discussed and summarised. Distribution data concerning exotic fish introduced into the Great Fish River by the Orange/Fish Tunnel has demonstrated that, with the exception of Clarias gariepinus, all remaining exotics have restricted distributions. Only Barbus aeneus appears to be extending its range down the drainage. Age, Growth, reproduction and condition data support the conclusion that, excluding B. aeneus and C. gariepinus, invasive species in the drainage are not performing well. Of the five invasive species (B. aeneus, Labeo capensis, L. umbratus, Gephyroglanis sclateri and Cyprinus carpio), only two have established populations (B. aeneus· and C. gariepinus). Labeo umbratus and C. carpio were present on the Great Fish River prior to the construction of the tunnel and populations of the species could not be distinguished. It was concluded that there is no evidence to suggest that exotic species introduced into the Great Fish River are having a deleterious effect on indigenous, endangered ichthyofauna. Monitoring of the drainage should be continued to ensure that a danger to indigenous species does not develop.

D.Sc. (Zoology) / The spermatogenesis of two freshwater species from South Africa, Barbus marequensis and B. polylepis, and three fresh water species from Israel, B. canis, B. longiceps and Capoeta damascina, was studied. A histological comparison of the process of spermatogenesis was undertaken. The breeding cycle of B. marequensis, B. polylepis, B. canis, B. longiceps and C. damascina was similar to the breeding cycle of B. aeneus and four phases occured within the cycle. The four distinctive phases are post spawning phase, rest phase, pre-spawning phase and the spawning phase (Vlok, 1986) . During the post spawning phase a decline in sperm development is observed and possible lisosomal activity is responsible for the resorption of sperm cells not shed during the spawning phase. The presence of collagen structure provides a distinctive character to the tissue of the testis. The resting phase is characterised by the absence of the lobular structure and the testis is dominated by the collagen tissue. The testis is small and unobtrusive in the abdominal cavities of both species. At the onset of the pre-spawning phase, the testis is filled with spermatogonia. The lobular structure becomes more prominent and the interstitial tissue can be distinguished. Later during the phase, the synchronised development of sperm cells in the cysts of the lobules can be observed, whilst sperm cells in adjacent lobules are in different stages of development. During the spawning phase the testis of all species studied contain mature sperm . cells in the lumens of the lobules.

Thesis (MSc)--University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Endemic redfin minnows of the Olifants River System are under threat of extinction, mainly because of alien predatory fish, water extraction and pollution. The taxonomic position and biogeography of redfins in relation to other barbs are uncertain. Enzyme electrophoresis was employed to assess genetic differentiation within and among certain Barbus and Pseudobarbus taxa. Fourteen enzymes were routinely recorded from muscle tissue extracts, yielding a differential number of active loci. The latter is due to different ploidy levels. Polyploid species showed fewer loci than the tetraploid or hexaploid number of loci expected just after the polyploidy event. Barbus aenus (hexaploid) expressed 6.2 % of the duplicated loci expected after the hexaploidy event. The tetraploid Pseudobarbus and serrated Barbus spp., expressed 37.5 % and 31.2 % of the duplicated loci expected after the tetraploidy event, respectively. This suggests that the ploidy event in Barbus aenus is more ancient than in the above-mentioned tetraploid barbs. The similar amount of diploidization in Pseudobarbus and tetraploid serrated Barbus spp. investigated, suggests a shared ploidy event between these two lineages. The number of active loci is apparently not a good indication of the ploidy level of African barbs, as hexaploid Barbus aenus expressed less active loci than the tetraploid barbs investigated. Initial screening of allozyme loci yielded fixed allele differences at 22 loci, but polymorphism only at seven. This suggests more potential for delineating species boundaries than for assessing gene flow and genetic diversity of populations. Genetic differentiation within and among populations of Pseudobarbus phlegethon, Barbus calidus and Barbus erubescens were investigated by screening 27 allozyme loci. A clear divergence between Pseudobarbus phlegethon populations from the Olifants and Doring Rivers was found (Nei's unbiased genetic distance = 0.355; F-statistic for subpopulation against the total = 0.877). Preference and adaptation for mountain stream habitat might explain the past isolation and subsequent divergence of Pseudobarbus phlegethon populations between these two rivers. Barbus calidus was genetically homogenous over its distribution (Nei's unbiased genetic distance = 0-0.009; F-statistic for subpopulation against the total = 0.135). It is proposed that Barbus calidus is better adapted than Pseudobarbus phlegethon to disperse through the main stream. The genetic divergence between Barbus calidus and Barbus erubescens (Nei's unbiased genetic distance = 0.063) is characteristic of conspecific populations. There seems to have been a loss of genetic diversity in redfin populations of the Doring River tributaries (heterozygosity = 0-0.01), compared to the redfin populations of the Olifants River tributaries (heterozygosity = 0.01- 0.04). From phylogenetic analysis ofallozyme characters, it seems as if the serrated tetraploid barbs from South Africa is the sister-group of Pseudobarbus, whilst Barbus anoplus was rejected as a sister-species for the latter. Barbus calidus, Barbus erubescens and Barbus serra were found to be closely related to each other. The ancestor of the redfins seems to have been present in the Cape Fold Mountains since at least the late- Tertiary. On the basis of distributional and allozyme information, conservation units reflecting historical divergence, historical gene flow and current gene flow were identified as Evolutionarily Significant Units, Historical Management Units and Current Management Units respectively. The Olifants and Doring River populations of Pseudobarbus phlegethon should be recognized as two distinct Evolutionarily Significant Units. Barbus calidus forms a separate Evolutionarily Significant Unit from Barbus erubescens. Twelve Historical Management Units and nineteen Current Management Units were recognized for redfins of the Olifants River System. The size of Current Management Units should be expanded and secured to prevent loss of genetic diversity. It is recommended that a recovery program of redfins should establish new populations of at least Barbus erubescens and Doring River Pseudobarbus phlegethon Evolutionarily Significant Unit. Centrarchids should be irradicated, as they are the main reason for the decline of red fins. / AFRIKAANSE OPSOMMING: Die endemiese rooivlerkies van die Olifantsriviersisteem word bedreig deur uitsterwing, hoofsaaklik as gevolg van uitheemse roofvisse, water onttrekking en besoedeling. Die taksonomiese posisie en biogeografie van rooivlerkies in verhouding met ander barbusse is onseker. Ensiemelektroferese is gebruik om genetiese differensiasie binne en tussen sekere Barbus en Pseudobarbus taksa te bepaal. Veertien ensieme, wat verskillende hoeveelhede aktiewe lokusse gelewer het, is op roetine basis waargeneem uit spierweefselekstrakte. Die verskillende hoeveelhede aktiewe lokusse wat waargeneem is, is as gevolg van verskillende ploied vlakke. Poliploïede spesies het minder lokusse getoon as wat verwag sou word net na die tetraploïede ofheksaploïede gebeurtenisse. Barbus aenus (heksaploïed) het 6.2% van die gedupliseerde lokusse, wat verwag sou word na die heksaploiede gebeurtenis, uitgedruk. Die tetraploïede Pseudobarbus en saagagtige Barbus spp. het 37.5% en 3l.2% respektiewelik van die gedupliseerde lokusse, wat verwag sou word na die tetraploïede gebeurtenis, uitgedruk. Dit dui daarop dat die ploïede gebeurtenis in Barbus aenus meer histories as in die bogenoemde tetraploïede barbusse. Die soortgelyke hoeveelheid diploïedisasie in Pseudobarbus en tetraploïede saagagtige Barbus spp. wat ondersoek is, dui op 'n moontlike gesamentlike ploïede gebeurtenis tussen hierdie twee evolusionêre lyne. Die aantal aktiewe lokusse blyk nie 'n goeie aanduiding van die ploïed vlakke van Afrika barbusse te wees nie, aangesien die heksaploïede Barbus aenus minder lokusse as die tetraploïede barbusse wat ondersoek is, getoon het. Aanvanklike analisering van allosiem lokusse het vaste alleel verskille in 22 lokusse opgelewer, maar slegs sewe het polimorfisme getoon. Dit dui op moontlike beter potensiaalom spesie-grense vas te stel, eerder as die bepaling van genevloei of genetiese diversiteit van populasies. Genetiese differensiasie binne en tussen populasies van Pseudobarbus phlegethon, Barbus calidus en Barbus erubescens is ondersoek deur 27 allosiem lokusse te analiseer. 'n Duidelike divergensie tussen Pseudobarbus phlegethon populasies van die Olifants- en Doring- riviere is gevind (Nei se onbevooroordeelde genetiese afstand = 0.355; F-statistiek vir subpopulasie teen die totaal = 0.877). Voorkeur en aanpassing vir bergstroom habitat, mag moontlik die historiese isolasie en daaropvolgende divergensie van Pseudobarbus phlegethon populasies tussen hierdie twee riviere verduidelik. Barbus calidus was geneties homogeen oor sy verspreiding (Nei se onbevooroordeelde genetiese afstand = 0-0.009; F-statistiek vir subpopulasie teen die totaal = 0.135). Dit word voorgestel dat Barbus calidus beter aangepas is as Pseudobarbus phlegethon om deur die hoofstroom te versprei. Die genetiese divergensie tussen Barbus calidus en Barbus erubescens (Nei se onbevooroordeelde genetiese afstand = 0.063) is kenmerkend van spesiespesifieke populasies. Dit lyk of daar 'n verlies aan genetiese diversiteit in rooivlerkie populasies vanafdie Doringrivier sytakke was (heterosigositeit = 0-0.01), in vergelyking met rooivlerkie populasies vanafdie Olifantsrivier sytakke (heterosigositeit = 0.01- 0.04). Uit die filogenetiese analise van allosiem kenmerke, blyk dit dat die saagagtige tetraploiede barbusse van Suid-Afrika die sistergroep van Pseudobarbus is, terwyl Barbus anoplus verwerp word as sisterspesie vir laasgenoemde. Dit is gevind dat Barbus calidus, Barbus erubescens en Barbus serra baie naverwant aan mekaar is. Dit lyk asof die voorvader van die rooivlerkies sedert ten minste die laat-Tersiêr in die Kaapse Plooiberge aanwesig was. Bewaringseenhede wat historiese divergensie, historiese genevloei en huidige genevloei voorstel, is geidentifiseer respektiewelik as Evolusionêre Beduidende Eenhede, Historiese Bestuurseenhede en Huidige Bestuurseenhede. Dit is gedoen op grond van verspreidings- en allosieminformasie. Die Olifants en Doring populasies van Pseudobarbus phlegethon moet as twee unieke Evolusionêre Beduidende Eenhede erken word. Barbus calidus en Barbus erubescens vorm aparte Evolusionêre Beduidende Eenhede. TwaalfHistoriese Bestuurseenhede en negentien Huidige Bestuurseenhede is geidentifiseer vir rooivlerkies van die Oifantsriviersisteem. Die grootte van Huidige Bestuurseenhede moet vergroot en beveilig word om verlies van genetiese diversiteit te vermy. Dit word voorgestel dat 'n herstelprogram van rooivlerkies moet poog om nuwe populasies, van ten minste Barbus erubescens en Doringrivier Pseudobarbus phlegethon Evolusionêre Beduidende Eenhede, te vestig. Baars en blouwang sonvis moet uitgeroei word, aangesien hulle die hoofoorsaak is vir die afname van rooivlerkies.

Evolutionary relationships between Barbus species have been already studied in the past, but most of the studies were based only on mitochondrial DNA, there is no detailed study based on nuclear markers. This is mainly due to the fact that Barbus species are tetraploid. The western part of Greece has been studied insufficiently, and moreover a greater diversity of freshwater ichthyofauna is expected here. Greece is one of the hotspots of biodiversity. Greek river system is characterized by a significant proportion of endemic species of freshwater fishes. In this work evolutionary relationships between Barbus species were studied, with focus on diversity of Greek lineages. The geographical distribution of Barbus species has been also revised. In total, 192 individuals were analyzed, 156 individuals were from 40 Greek localities and another 36 Barbus species were from other areas of distribution of the genus. Dataset was supplemented with avaible published sequences. For the phylogenetic analysis mitochondrial gene cytochrom b, 1st intron of ribosomal protein gene S7 and 2nd intron of gene beta-actin were used. For both nuclear markers paralog-specific primers were used. The analysis of cytochrom b revealed several well-supported lines: 1. species of southwestern Mediterranean (B. meridionalis and B....

The construction of the Katse dam was completed in 1996. The dam started filling in 1995 and reached full capacity in early 1998. It forms part of Phase 1A of the Lesotho Highlands Water Project (LHWP). The main aim of the project is to provide revenue to Lesotho, by transferring water from the catchment of the Senqu (Orange) River in Lesotho to South Africa’s major industrial and population centres. During the implementation of the project, an estimated 130 people were displaced. However, the most serious impact has been the loss of the traditional way of life in the form of arable and grazing land as a consequence of inundation. One of the obligations of the project is to ensure that the standard of living of those affected by the project is not impaired. The Lesotho Highlands Development Authority identified a number of rural development projects, which included fisheries development. This study is an integral part of fisheries development in Lesotho. The aim of this study was to gain an understanding of the biology and the demographics of the fish species in the lake. This information would serve as the basis for the development of a management plan for the sustainable utilisation of the fisheries resources. To achieve this aim, the following specific objectives were addressed: 1. Investigation of the biology of the three principal species. 2. Description of the key population parameters (growth, mortality & recruitment). 3. Investigation of the distribution and relative abundance of the three species. The three principal species in the Katse dam are rainbow trout, Oncorhynchus mykiss, and the two cyprinids, Barbus aeneus and Labeo capensis. Sectioned otoliths were used to age O. mykiss while both otoliths and scales were used to age B. aeneus and L. capensis. Marginal zone analysis revealed that slow growth was experienced in winter for the three species. The maximum-recorded age for both B. aeneus and L. capensis was 12 years while O. mykiss reached 4 years. There was no significant difference in growth rates of the different sexes and growth was best described by the 3 parameter Von-Bertalanfy growth model as Lt = 603[1-e⁻°·¹⁵⁽t ⁺ °·°³⁾] for B. aeneus, Lt = 526[1-e⁻°·²¹⁽t ⁺ °·⁹⁾] for O. mykiss and Lt = 521[1-e⁻°·¹⁷⁽t ⁺ °·²¹⁾] for L.capensis. Male and female O. mykiss attained 50% sexual maturity (Lm₅₀) at 235 and 275mm FL, respectively. There was no difference in Lm₅₀ for male and female B. aeneus and L. capensis. B. aeneus reached sexual maturity at 285mm FL while L. capensis reached sexual maturity at 244mm. Both cyprinid species spawned in summer (November to January) while O. mykiss spawned in winter (May to August). The mean total mortality rate (Z) estimated from catch curves and Butterworth et al (1989) equation was 0.72 yr⁻¹ for B. aeneus, 0.62 yr⁻¹ for L. capensis and 1.32 yr⁻¹ for O. mykiss. The estimate of natural mortality was 0.41 yr⁻¹ for both B. aeneus and L. capensis and 0.81yr⁻¹ for O. mykiss.

Nomenclature: Sensitivity to anesthetic clove oil different species of adult fish Sensitivity of breeding fishes of various species (Siberian sturgeon, Brook trout, Black carp- young breeding fishes) for anesthetic clove oil was probed by the tests performed at temperature in the period of stripping (apart from Brook trout and Black carp). The tests were carried out with 17 fish species- with cyprinids (Ide- golden form, Common dace, European Chub, Sneep, Barbel, Grass carp, Black carp, bighead carp), salmonids (Brown trout, Rainbow trout, Brook trout), thymallinae (Grayling), pikes (Northern pike), perches (Pike-perch), sturgeons (Siberian sturgeon, Beluga) and airbreathing catfishes (North African catfish). Using concentration of 0,03ml.l-1 (Brown trout, Rainbow trout, Brook trout, Grayling, Pike, Pike perch, Ide, Dace, Chub, Barbel and Sneep), 0,04ml.l-1 (Grass carp, Black carp, Bighead carp), 0,05ml.l-1 (North African catfish) and 0,07ml.l-1 (Siberian sturgeon, Beluga) the IIb. Phase has been achieved, which is sufficient for manipulation with fishes during stripping. The anesthesia subsided after time period of 10-17 minutes (12 species) at the majority of fishes and at others after interval of 20-26 minutes (5 species). The results confirmed concentrations recommended for salmodis and Grayling (0,025-0,03ml.l-1), sturgeons (0,07ml.l-1), and North African catfish (0,05ml.l-1). At the majority of cyprinids (apart from Grass carp, Black carp and Bighead carp), the concentration used was lower than recommended (0,04-0,05ml.l-1)

M.Sc. (Aquatic Health) / Yellowfish and specifically Labeobarbus marequensis are a charismatic species targeted by anglers throughout South Africa. Their population are limited to the north-western parts of the country including the lower reaches of the Crocodile River that flows through the Kruger National Park (KNP). Despite conservation efforts the Crocodile River in the KNP is still highly impacted. The effect of these impacts on the ecosystem is largely unknown. The main aim of the study was to determine the influence of changing water quantity and quality in the Crocodile River on adult L. marequensis. This was achieved by evaluating altered flows (discharge) on the behaviour of adult L. marequensis in the Crocodile River using biotelemetry over a two year period. The influence of altered water quality was assessed using metal bioaccumulation as an indicator of metal exposure in L. marequensis, Clarias gariepinus and Hydrocynus vittatus in the Crocodile and Sabie Rivers during a high and low flow season. Biotelemetry was used on 16 L. marequensis and 12 H. vittatus to determine the habitat use and movement responses of the species. Fish were tagged with Advanced Telemetry Systems (ATS) and Wireless Wildlife (WW) tags and tracked remotely and manually. Home ranges were determined using Arc GIS ®, Habitat uses were analyzed using Windows Excel (© 2011, Microsoft inc.). Environment variables recorded were scored as primary and secondary and then combined with a weighting variable 2:1 ratio (primary variable: secondary variable). A mixed-model analysis of variance (ANOVA) approach with a random co-efficients model and Akaike’s information criteria (AIC) were used to test for significance. Analyses were conducted using SAS version 9 (SAS institute, Cary, NC)...

M.Sc. (Aquatic Health) / This study took place in the Groot Marico River catchment situated in the North West Province of South Africa. The catchment holds many rivers and associated tributaries that are home to a variety fish species with some being endemic to the catchment. A small minnow species, the Marico barb Barbus motebensis was chosen as the study species due to it being endemic to the catchment with limited information available on them with regards to their reproductive status in terms of their reproductive timing and number of potential offspring. Two tributaries from the upper Groot Marico River were chosen as sampling sites for the collection of B. motebensis across the four seasons. These two tributaries presented two genetically different populations, which allowed for a comparison of the findings to ascertain whether any differences in reproductive aspects occurred between the two tributary populations during the different seasons. The Marico barb is under threat from predation pressure posed by the largemouth bass Micropterus salmoides introduced into the system. Micropterus salmoides is a known alien invasive fish that eats any animal food item it encounters, especially fish. Due to B. motebensis and M. salmoides sharing the same habitat preferences, B. motebensis stands little chance of survival in the same water inhabited by the predatory M. salmoides. Many studies have shown bass to drive other fish species to the point of extirpation from a system through predation pressure. Currently B. motebensis is classified as vulnerable on the Red List of Threatened Species, although this status may be further compromised with the presence of M. salmoides...

To contribute to the understanding of the invasion biology of common carp Cyprinus carpio in southern Africa, this thesis investigated the life history, relative abundance, long-term population demographics and trophic niche utilisations of non-native common carp C. carpio in relation to two endemic cyprinids, Orange River mudfish Labeo capensis and smallmouth yellowfish Labeobarbus aeneus in South Africa‟s largest impoundment, Lake Gariep. The growth zone deposition rates in astericus otoliths of the three species were validated as biannual for C. carpio and as annual for L. capensis and L. aeneus, which allowed for reliable estimation of lengths-at-age upon which growth, age-at-maturity and mortality rates could be estimated. Cyprinus carpio exhibited fast growth, matured relatively early at two years of age and attained a maximum age of seven years. Labeo capensis grew significantly slower, but attained older ages of up to 12 years. Females showed notably delayed maturation at approximately six years of age. The life history parameter estimates for L. aeneus were similar to those of L. capensis. These species-specific life history characteristics contributed to a substantially higher population growth potential of C. carpio compared to L. capensis and L. aeneus. Delta-lognormal and delta-gamma Generalized Linear Models (GLMs) were used to analyse patterns of relative abundance of L. capensis, L. aeneus and C. carpio. The application of these GLMs was necessary to account for large proportions of zeros and strong skewness in the catch-per-unit-effort (CPUE) from experimental gillnet and fisheries-dependent angler surveys. Confidence intervals around predicted abundance indices were obtained through the development of a generalised parametric bootstrap procedure. The resulting standardised abundance indices were coupled with results from analysis of stable isotope ratios of fish tissues and potential food resources and revealed that C. carpio was mainly confined to soft-bottom habitats, where it predominantly foraged on benthic invertebrates. Labeo capensis was abundant in a wide range of benthic habitats and was consumed basal food resources such as detritus. Labeobarbus aeneus was found to feed mostly on pelagic zooplankton. There were no significant interspecific differences in trophic niche space, suggesting limited resource competition among the three species. Standardised historical and contemporary gillnet CPUE data indicated slow population growth rates of L. capensis and L. aeneus during the first ten years postimpoundment, but showed high biomass levels some four decades after impoundment. These results could be corroborated by stochastic age-structured production model (ASPM) simulations. In contrast to the two endemic species, the gillnet CPUE of C. carpio showed a clear „boom and bust‟ pattern, which, based on ASPM simulations, could be best explained by increased food availability during the first five years postimpoundment, followed by suboptimal conditions thereafter. Together, these results provided evidence that the establishment of the C. carpio population did not prevent the slow but successful long-term establishment of the two large endemic cyprinids. Both endemic fishes revealed specialised feeding within the impoundment.

This dissertation focused in the comprehensive analysis of the capabilities of some non-tested types of Artificial Neural Networks, specifically: the Probabilistic Neural Networks (PNN) and the Multi-Layer Perceptron (MLP) Ensembles. The analysis of the capabilities of these techniques was performed using the native brown trout (Salmo trutta; Linnaeus, 1758), the bermejuela (Achondrostoma arcasii; Robalo, Almada, Levy & Doadrio, 2006) and the redfin barbel (Barbus haasi; Mertens, 1925) as target species. The analyses focused in the predictive capabilities, the interpretability of the models and the effect of the excess of zeros in the training datasets, which for presence-absence models is directly related to the concept of data prevalence (i.e. proportion of presence instances in the training dataset). Finally, the effect of the spatial scale (i.e. micro-scale or microhabitat scale and meso-scale) in the habitat suitability models and consequently in the e-flow assessment was studied in the last chapter. / Esta tesis se centra en el análisis comprensivo de las capacidades de algunos tipos de Red Neuronal Artificial aún no testados: las Redes Neuronales Probabilísticas (PNN) y los Conjuntos de Perceptrones Multicapa (MLP Ensembles). Los análisis sobre las capacidades de estas técnicas se desarrollaron utilizando la trucha común (Salmo trutta; Linnaeus, 1758), la bermejuela (Achondrostoma arcasii; Robalo, Almada, Levy & Doadrio, 2006) y el barbo colirrojo (Barbus haasi; Mertens, 1925) como especies nativas objetivo. Los análisis se centraron en la capacidad de predicción, la interpretabilidad de los modelos y el efecto del exceso de ceros en las bases de datos de entrenamiento, la así llamada prevalencia de los datos (i.e. la proporción de casos de presencia sobre el conjunto total). Finalmente, el efecto de la escala (micro-escala o escala de microhábitat y meso-escala) en los modelos de idoneidad del hábitat y consecuentemente en la evaluación de caudales ambientales se estudió en el último capítulo. / Aquesta tesis se centra en l'anàlisi comprensiu de les capacitats d'alguns tipus de Xarxa Neuronal Artificial que encara no han estat testats: les Xarxes Neuronal Probabilístiques (PNN) i els Conjunts de Perceptrons Multicapa (MLP Ensembles). Les anàlisis sobre les capacitats d'aquestes tècniques es varen desenvolupar emprant la truita comuna (Salmo trutta; Linnaeus, 1758), la madrilla roja (Achondrostoma arcasii; Robalo, Almada, Levy & Doadrio, 2006) i el barb cua-roig (Barbus haasi; Mertens, 1925) com a especies objecte d'estudi. Les anàlisi se centraren en la capacitat predictiva, interpretabilitat dels models i en l'efecte de l'excés de zeros a la base de dades d'entrenament, l'anomenada prevalença de les dades (i.e. la proporció de casos de presència sobre el conjunt total). Finalment, l'efecte de la escala (micro-escala o microhàbitat i meso-escala) en els models d'idoneïtat de l'hàbitat i conseqüentment en l'avaluació de cabals ambientals es va estudiar a l'últim capítol. / Muñoz Mas, R. (2016). Multivariate approaches in species distribution modelling: Application to native fish species in Mediterranean Rivers [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/76168 / TESIS