The Indiana Bat (Myotis sodalis) population had decreased by 56% between 1967 and 2006. In summer 2006, a mysterious disease called White Nose Syndrome was first identified. Since then, the disease killed almost one million bats in North America. Many Biologists believe that both the population decrease before the appearance of the disease and WNS are associated with climate. In a joined effort with Yellowstone Ecological Research Center (YERC), US Fish and Wildlife Service (USFWS) and NASA Terrestrial Observation and Prediction System (TOPS), our study is a partial population viability analysis which aims to establish a link between bat population dynamic and climate before the appearance of WNS. / Biostatistics

In Chapter 1, the literature relating to rabies virus and the rabies like lyssaviruses is reviewed. In Chapter 2 data are presented from 1170 diagnostic submissions for ABLV testing by fluorescent antibody test (Centocor FAT). All 27 non-bat submissions were ABLV-negative. Of 1143 bat accessions 74 (16%) were ABLV-positive, including 69 of 974 (7.1%) flying foxes (Pteropus spp.), 5 of 7 (71.4%) Saccolaimus flaviventris (Yellow-bellied sheathtail bats), none of 151 other microchiropteran bats, and none of 11 unidentified bats. Statistical analysis of data from 868 wild Black, Grey-headed, Little Red and Spectacled flying foxes (Pteropus alecto, P. poliocephalus, P. scapulatus, and P. conspicillatus) indicated that three factors; species, health status and age were associated with significant (p&lt 0.001) differences in the proportion of ABLV-positive bats. Other factors including sex, whether the bat bit a person or animal, region, year, and season submitted, were not associated with ABLV. Case data for 74 ABLV-positive bats, including the circumstances in which they were found and clinical signs, is presented. In Chapter 3, the aetiological diagnosis was investigated for 100 consecutive flying fox submissions with neurological signs. ABLV (32%), spinal and head injuries (29%), and neuro-angiostrongylosis (18%) accounted for most neurological syndromes in flying foxes. No evidence of lead poisoning was found in unwell (n=16) or healthy flying foxes (n=50). No diagnosis was reached for 16 cases, all of which were negative for ABLV by TaqMan® PCR. The molecular diversity of ABLV was examined in Chapter 4 by sequencing 36 bases of the leader sequence, the entire N gene, and start of the P gene of 28 isolates from pteropid bats and 3 isolates from Yellow-bellied sheathtail (YBST) bats. Phylogenetic analysis indicated all ABLV isolates clustered together as a discrete group within the Lyssavirus genera closely related to rabies virus and European bat lyssavirus-2 isolates. The ABLV lineage consisted of two variants; one (ybst-ABLV) consisted of isolates only from YBST bats, the other (pteropid-ABLV) was common to Black, Grey-headed and Little Red flying foxes. No associations were found between the sequences and either the geographical location or year found, or individual flying fox species. In Chapter 5, 15 inocula prepared from the brains or salivary glands of naturally-infected bats were evaluated by intracerebral (IC) and footpad (FP) inoculation of Quackenbush mice in order to select and characterize a highly virulent inoculum for further use in bats (Inoculum 5). In Chapter 6, nine Grey-headed flying foxes were inoculated with 105.2 to 105.5 MICED50 of Inoculum 5 divided into four sites, left footpad, pectoral muscle, temporal muscle and muzzle. Another bat was inoculated with half this dose divided into the footpad and pectoral muscle only. Seven of 10 bats developed clinical disease of 1 to 4 days duration between PI-days 10 and 19 and were shown to be ABL-positive by FAT, HAM immunoperoxidase staining, virus isolation in v mice, and TaqMan PCR. Five of the seven bats displayed overt aggression, one died during a seizure, and one showed intractable agitation, pacing, tremors, and ataxia. Viral antigen was demonstrated throughout the central and peripheral nervous systems and in the epithelial cells of the submandibular salivary glands (n=4). All affected bats had mild to moderate non-suppurative meningoencephalitis and severe ganglioneuritis. No ABLV was detected in three bats that remained well until the end of the experiment on day 82. One survivor developed a strong but transient antibody response. In Chapter 7, the relative virulence of inocula prepared from the brains and salivary glands of experimentally infected flying foxes was evaluated in mice by IC and FP inoculation and TaqMan assay. The effects in mice were correlated to the TaqMan CT value and indicated a crude association between virulence and CT value that has potential application in the selection of inocula. In Chapter 8, 36 Black and Grey-headed flying foxes were vaccinated with one (day 0) or two (+ day 28) doses of Nobivac rabies vaccine and co-vaccinated with keyhole limpet haemocyanin (KLH). All bats responded to the Nobivac vaccine with a rabies-RFFIT titer &gt 0.5 IU/mL that is nominally indicative of protective immunity. Plasma from bats with rabies titres &gt 2 IU/mL had cross-neutralising ABLV titres &gt 1:154. A specifically developed ELISA detected a strong but transient response to KLH.

Thesis (doctoral)--Ludwig-Maximilians-Universität München, 2008. / Title from PDF title page (viewed on Nov. 12, 2008). Includes vita. Includes bibliographical references (p. [111]-131).

Thesis (Ph. D.)--Ludwig-Maximilians-Universität München, 2006. / Title from PDF title page (viewed on June 4, 2006). Includes bibliographical references.

With the loss or modification of natural roosting habitat afforded by caves, abandoned mines have assumed increased importance as surrogate roosting sites for Townsend's big-eared bats (Corynorhinus townsendii) and other chiropteran species. However, increasing concerns for human safety have led to accelerated programs for mine closure. In efforts to protect roosting sites in mines showing significant bat activity, "bat compatible" gates are installed, thus allowing continued access to mine workings. Aside from ensuring public safety, these structures afford protection from disturbance to roosting bats. To date few posting-gating studies have been conducted to obtain information on the effects of these structures on bat behavior and roost suitability. I evaluated the effects of gating on bat flight patterns at maternity colonies in two previously gated (reference) and two ungated mines, the latter (treatment) being gated with roundbar Manganal steel gates in the second year of the study. I also monitored four gated and three treatment mines to determine the potential effects of reclamation on internal microclimate. Overall circling activity increased > 6-fold at the portals of treatment mines following gating. Indices of crowding and frequency of bat-gate collisions were significantly higher in previously gated and increased substantially in treatment mines following reclamation. Gates appeared especially hazardous to subadults during initial-volancy periods. Increased activity of bats and collisions with gates at mine portals may amplify vulnerability to potential predators. Changes in internal mine microclimates, specifically increased ranges between minima and maxima in temperature and vapor pressure deficits following reclamation varied among treatment mines as a function of the number of mine openings. Generally, gated mines with multiple openings experienced greater changes in these parameters than those with single openings. Additional studies of bat-compatible gates are needed to elucidate possible long-term effects of these structures on Townsend's big-eared bats.

No description available.

Bats have many impressive flight characteristics such as the ability to rapidly change direction, carry substantial loads, and maintain good flight efficiency. For several years, researchers have been working towards an understanding of the specific aerodynamic phenomena which relate the unique wing structure of bats to their flight abilities. Computational fluid dynamics, a powerful tool used extensively across aerospace research, has led to substantial progress in the understanding of insect flight. However, due to technical challenges, numerical simulation has seen limited use in bat flight research. For this research, we develop, validate, and apply computational modeling techniques to three modes of bat flight: straight flight, sweeping turn, and U-turn maneuver. 3D kinematic data collection was achieved using a 28 camera multi-perspective optical motion capture system. The calibration of the cameras was conducted using a multi-camera self-calibration method. Point correspondences between cameras and frames was achieved using a human-supervised software package developed for this project. After the collection of kinematic data, we carried out aerodynamic flow simulations using the incompressible Navier-Stokes solver, GenIDLEST. The immersed boundary method (IBM) was used to impose moving boundary conditions representing the wing kinematics. Validation of the computational model was preformed through a grid independence study as well as careful evaluation of other relevant simulation parameters. Verification of the model was performed by comparing simulated aerodynamic loads to the expected loads based on the observed flight trajectories. Additionally, we established that we had a sufficient resolution of the wing kinematics, by calculating the sensitivity of the simulation results to the number of kinematic markers used during motion capture. For this study, three particular flights are analyzed—a straight and level flight, a sweeping turn, and a sharp 180 degree turn. During straight flight, typical flight velocities observed in the flight tunnel were 2-3 m/s resulting in a Reynolds number of about 12,000. Lift generation occurred almost exclusively during the downstroke, and peaks mid-downstroke. At the beginning of each downstroke, the effective angle of attack of the wings transitions from negative to positive and a leading edge vortex (LEV) quickly forms. LEVs are known to augment lift generation in flapping flight and allow lift to remain high at large angles of attack. During the end of each downstroke, the LEVs break up and lift drops substantially. As the wingbeat cycle transitions from downstroke to upstroke, the wings rotate such that the wing chordline is vertical as the wing moves upward. This wing rotation is critical for mitigating negative lift during the upstroke. Many of the basic flight mechanisms used for straight flight—i.e. LEV formation, wing rotation during upstrokes—were also observed during the sweeping turn. In addition, asymmetries in the wing kinematics and consequently the aerodynamics were observed. Early in the turn, the bank angle was low and elevated levels of thrust were generated by the outer wing during both the upstroke and downstroke causing a yaw moment. As the bat moved towards the middle of the turn, the bank angle increased to 20-25 degrees. Although the bank angle remained nominally constant during the middle and later portion of the turn, there was variation within each wingbeat cycle. Specifically, the bank angle dropped during each upstroke and subsequently was recovered during each downstroke as a consequence of elevated lift on the outer wing. Banking served to redirect the net force vector laterally causing a radial, centripetal force. Considering the mass of the bat, the nominal flight velocity, and the radius of curvature, the magnitude of the radial force fully explained the expected centripetal acceleration during the middle and later portion of the turn. Over the entire turn, yaw was found to be important in initiating the turn while banking was more important during the middle part of the turn. Over the course of 5 wingbeat cycles, the change in bearing angle (direction of flight) was about 45 degrees. Analysis of the U-turn flight showed many of the same characteristics as were observed during the sweeping turn, as well as a few key differences. The bat's ability to rotate its body rapidly appears to be more limited than its ability to change its trajectory. For this reason, the yaw rotation began about one to two cycles before the rapid bearing angle change and was stretched out over several wingbeat cycles. At the apex of the U-turn, the bat combined a high roll angle with a low flight velocity magnitude to very rapidly redirect its bearing direction and negotiate a low radius of curvature flight trajectory. Increases in roll angle occurred almost exclusively during the downstrokes, while both the upstroke and downstroke were active in generating yaw. Elevated thrust on the left outer wing during the end of the upstroke was observed throughout the flight, and elevated drag on the right inside wing did not appear to have an impact on the turn. We hope that this project motivates and facilitates further computational analysis into bat flight aerodynamics. Additionally, the data and findings will be useful for applications such as the design of bioinspired MAVs or flexible membrane energy harvesting technology. / Doctor of Philosophy / Bats have many impressive flight characteristics such as the ability to rapidly change direction, carry substantial loads, and maintain good flight efficiency. A better understanding of the physics of how bats fly can help scientists and engineers build more maneuverable, quieter, and more efficient bioinspired micro air vehicles. This engineering approach leverages the incredible capabilities observed in nature, but requires detailed knowledge of the animal as a prerequisite. Computational fluid dynamics, a powerful tool used extensively across aerospace research, has led to substantial progress in the understanding of animal flight broadly. However, due to technical challenges, numerical simulation has seen limited use in bat flight research. For this research, we develop, validate, and apply computer modeling techniques to the investigation of bat flight aerodynamics. Three particular modes of flight were analyzed—a straight and level flight, a sweeping turn, and a sharp 180 degree turn. During straight flight, typical flight velocities observed in the flight tunnel were 2-3 m/s. Lift generation, the force keeping the bat aloft, occurred almost exclusively during the downstroke, and peaks mid-downstroke. As the wing flap transitions from downstroke to upstroke, the wings rotate such that the wing is vertical as it moves upward. This wing rotation is critical for maximizing lift force during flight. During the sweeping turn, asymmetries in the wing kinematics and consequently the aerodynamics were observed. Early in the turn, the bank angle was low and elevated levels of thrust were generated by the outer wing during both the upstroke and downstroke causing rotation of the bat. As the bat moved towards the middle of the turn, the bank angle increased to 20-25 degrees. Banking served to redirect the net force vector laterally causing a turning force. Over the course of 5 wingbeat cycles, the change in direction of flight was about 45 degrees. Analysis of the U-turn flight showed many of the same characteristics as were observed during the sweeping turn, as well as a few key differences. At the apex of the U-turn, the bat combined a high roll angle with a low flight velocity magnitude to very rapidly redirect its bearing direction and negotiate a low radius of curvature flight trajectory. We hope that this project motivates and facilitates further computer simulations studying bat flight aerodynamics. Additionally, the data and findings will be useful for applications such as the design of bioinspired MAVs or flexible membrane energy harvesting technology.

As human population grows and develops, more urban areas are expanding. Urbanisation has many impacts on the natural environment and one understudied pollutant is artificial light at night. The aims of this thesis were to examine the impacts of street lighting on bats and investigate the exposure of British bat species to artificial light at night and explored the mitigation option of part-night lighting. The current exposure of British bat species to artificial lighting was assessed using roost locations and population sizes from a long-term dataset (1997-2012) from the Bat Conservation Trust’s National Bat Monitoring Programme on seven bat species (Eptesicus serotinus, Myotis nattereri, Pipistrellus pipistrellus, P. pygmaeus, Plecotus auritus, Rhinolophus hipposideros and R. ferrumequinum). These data were combined with satellite imagery in roost sustenance zones and home ranges. Bat roosts were found in areas with brighter light levels than random locations for P. pipistrellus, P. pygmaeus and Plecotus auritus. Species that forage around streetlights (P. pipistrellus and P. pygmaeus) had significantly higher light levels in the landscape around their roosts than species which avoid street lit areas (R. hipposideros, M. nattereri and P. auritus). Colony size was negatively correlated with light levels. This study highlights that different species have different requirements in the landscapes around their roosts. To investigate landscapes effects of artificial light at night on the understudied light avoiding species R. ferrumequinum, eight maternity roosts were surveyed to explore the interaction between habitat features and street lighting. At each maternity roost, bat detectors were deployed at 25 paired street lit and dark locations. Street lighting had a significant negative effect on bat activity. Locations closest to the maternity roost had higher bat activity than those further away and road type had a significant effect on bat activity, with the highest bat activity recorded at minor roads compared with A and B roads. These results highlight the large negative impact street lighting can have on bat activity patterns and the need for mitigation. Several mitigation strategies have been suggested to combat the effects of artificial light at night but few have been tested. One of these suggestions is to restrict the hours of lighting through the night, often called part-night lighting. Part-night lighting has been implemented by many local authorities, often switching the lights off after midnight and switching them back on before dusk. To explore the effects of part night lighting on bats, the hourly patterns of activity for R. ferrumequinum were studied. Bat activity was bimodal, with a peak in the first few hours after sunset followed by a smaller peak before sunrise. To capture more than 50% of bat activity during the dark period of the night, street lights would be required to switch off before 11pm. To explore this further, a before-and-after study of part-night lighting was conducted at towns across Devon. Following the conversion from full-night lighting to part-night lighting, switching street lights off at 2 am, there was a significant reduction in P. pipistrellus and a significant increase for P. pygmaeus and Nyctalus noctule activity. Although part-night lighting is not often operational during peak activity periods for bat species, reducing the duration of lighting at night has impacts on activity patterns for several species. This thesis shows that artificial light at night has impacts on bats across the landscapes around their roosts. Artificial lighting has impacts for species in different ways, depending on whether they forage around street lights or avoid street lit areas. For species that avoid street lit areas such as R. ferrumequinum, street lighting can have very significant negative impacts on the availability of areas around their roosts. This highlights the need for conservation measures to reduce impacts of artificial lighting. Although mitigation schemes such as part-night lighting may help to minimize impacts of nighttime lighting, more tailored schemes for bats should devised to achieve greater conservation impacts.

This study examines in detail the various aspects of the segmentation process as applied by Obrecht and Josquin to the chanson Malheur me bat, especially the effect of this process on the structure of each composer's respective mass. Although musical aspects such as cadences and mode have varying degrees of influence on the structure of these two masses, the primary influence is the establishment of proportional relationships that occur as a result of the segmentation process. Sources of previous music research frequently point out that Obrecht's Mass utilizes both the Phrygian and Aeolian modes, while in Josquin's Mass the Phrygian mode is the firmly established mode throughout. Since segments in Obrecht's Mass are usually not connected to one another, strong cadences frequently occur at the end of the segments throughout. On the other hand, since the segments in Josquin's Mass are usually connected to one another, weak internal cadences frequently occur throughout, with strong cadences reserved for the end of sections.

No description available.