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1	Determination of antibody affinity and kinetic binding constants in Gyrolab Bioaffy microfluidic CD Karlsson, Mikael January 2008 (has links) <p>Studies of binding reactions are of highest importance in a vast number of areas of biomedicine and biotechnology. A demand for fast and accurate small-volume measurements grows stronger, partly due to the development of therapeutic antibodies. In this report, a novel method for studies of binding reactions of antibodies is described. The use of a microfluidic platform shows promising results in determination of affinity binding constants.</p><p>Affinities between 1E-09 and 1E-11 M have been determined for four TSH antibodies. Reproducibility tests give a CV below 10%, using different Gyrolab instruments and microfluidic CD:s. The method carries the advantages of using solution-based measurements of unmodified molecules. Also an initial proof-of-concept for measurement of binding reaction rate constants shows further usage of the method. The kinetic association rate constant has been determined to 2E+06 M-1s-1 for one antibody. The possibility of using this method for screening of antibody libraries is also discussed.</p> Affinity constants Equilibrium dissociation constants Kinetic rate constants Immunoassay Monoclonal antibodies Microfluidics Gyrolab Bioaffy Microlaboratory Compact disc Bioengineering Bioteknik
2	Determination of antibody affinity and kinetic binding constants in Gyrolab Bioaffy microfluidic CD Karlsson, Mikael January 2008 (has links) Studies of binding reactions are of highest importance in a vast number of areas of biomedicine and biotechnology. A demand for fast and accurate small-volume measurements grows stronger, partly due to the development of therapeutic antibodies. In this report, a novel method for studies of binding reactions of antibodies is described. The use of a microfluidic platform shows promising results in determination of affinity binding constants. Affinities between 1E-09 and 1E-11 M have been determined for four TSH antibodies. Reproducibility tests give a CV below 10%, using different Gyrolab instruments and microfluidic CD:s. The method carries the advantages of using solution-based measurements of unmodified molecules. Also an initial proof-of-concept for measurement of binding reaction rate constants shows further usage of the method. The kinetic association rate constant has been determined to 2E+06 M-1s-1 for one antibody. The possibility of using this method for screening of antibody libraries is also discussed. Affinity constants Equilibrium dissociation constants Kinetic rate constants Immunoassay Monoclonal antibodies Microfluidics Gyrolab Bioaffy Microlaboratory Compact disc Bioengineering Bioteknik
3	Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab Johansson, Fredrik January 2011 (has links) The number of monoclonal antibodies approved for therapeutic use has increased rapidlyover the last decade. As a consequence, precise and robust kinetic characterization techniquesare crucial in order to select the best suitable candidates. A kinetic characterization methodwas developed in Gyrolab with automated sample transfers. The characterization wasperformed in solution in a mixing CD, containing an integrated nanoliter mixing chamberwith affinity binding columns. Association rate constants were determined for four anti-TSHantibodies with values ranging from 3x105 M-1s-1 to 10x105 M-1s-1. The antibodies wereranked according to kass. Reproducibility Affinity association rate constant Bioaffy characterization Gyrolab immunoassay kinetics microfluidics monoclonal antibody screening Bioengineering Bioteknik Medical engineering Medicinsk teknik Enzyme engineering Enzymteknik Immunology Immunologi

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