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1	Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab Johansson, Fredrik January 2011 (has links) The number of monoclonal antibodies approved for therapeutic use has increased rapidlyover the last decade. As a consequence, precise and robust kinetic characterization techniquesare crucial in order to select the best suitable candidates. A kinetic characterization methodwas developed in Gyrolab with automated sample transfers. The characterization wasperformed in solution in a mixing CD, containing an integrated nanoliter mixing chamberwith affinity binding columns. Association rate constants were determined for four anti-TSHantibodies with values ranging from 3x105 M-1s-1 to 10x105 M-1s-1. The antibodies wereranked according to kass. Reproducibility Affinity association rate constant Bioaffy characterization Gyrolab immunoassay kinetics microfluidics monoclonal antibody screening Bioengineering Bioteknik Medical engineering Medicinsk teknik Enzyme engineering Enzymteknik Immunology Immunologi