Fung, Chun Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 121-135). / Abstracts in English and Chinese. / Declaration --- p.i / Acknowledgement --- p.ii / Abbreviations --- p.iii / Abstract in English --- p.v / Abstract in Chinese --- p.vii / Table of Contents --- p.ix / List of Figures --- p.xii / Chapter Chapter 1 - --- Introduction / Chapter 1.1 --- Genus Cordyceps --- p.1 / Chapter 1.2 --- Cordyceps militaris --- p.4 / Chapter 1.3 --- Biological Functions and Chemical Constituents of Cordyceps militaris --- p.9 / Chapter 1.4 --- "Human Bronchial Epithelial Cell Line, 16HBE14o-" --- p.13 / Chapter 1.5 --- Ion Transport in Human Bronchial Epithelial Cells --- p.16 / Chapter 1.6 --- Objectives of the Experiments --- p.20 / Chapter Chapter 2 - --- Materials and Methods / Chapter 2.1 --- Solutions and Chemicals --- p.21 / Chapter 2.2 --- Preparation of Hot Water Cordyceps militaris Extract --- p.22 / Chapter 2.3 --- Culture of Cells --- p.23 / Chapter 2.4 --- Short-Circuit Current (lsc) Measurement --- p.24 / Chapter 2.5 --- Short-Circuit Current (lsc) Measurement in Nystatin-Permeabilized Monolayer --- p.29 / Chapter 2.6 --- Measurements of [Ca2+]i --- p.31 / Chapter 2.7 --- Measurement of PKA Activity --- p.36 / Chapter 2.8 --- Statistical Analysis --- p.37 / Chapter Chapter 3 - --- Results / Chapter 3.1 --- Regulation of Ion Transport in 16HBE14o- Cells by CM Water Extract --- p.38 / Chapter 3.1.1 --- Dose-Dependent Relationship of CM Water Extract --- p.39 / Chapter 3.1.2 --- "Involvement of CI"" Transport in CM-induced lsc Response" --- p.42 / Chapter 3.1.3 --- Involvement of K+ channels in CM-induced lsc Response --- p.47 / Chapter 3.1.4 --- Involvement of Adenylate Cyclase/cAMP/Protein Kinase A Pathway in CM-induced lsc Response --- p.52 / Chapter 3.1.5 --- Involvement of Ca2+-Dependent Pathway in CM-induced lsc Response --- p.57 / Chapter 3.1.6 --- "Effect of CM Extract on Apical CI"" Current and Basolateral K+ Current in Nystatin-Permeabilized Epithelia" --- p.61 / Chapter 3.1.7 --- Effect of CM Extract on PKA Activity --- p.67 / Chapter 3.2 --- Regulation of Ion Transport in 16HBE14o- Cells by Cordycepin --- p.70 / Chapter 3.2.1 --- Dose-Dependent Relationship of Cordycepin --- p.70 / Chapter 3.2.2 --- "Involvement of CI"" Transport in Cordycepin-induced lsc Response" --- p.73 / Chapter 3.2.3 --- Involvement of K+ channels in Cordycepin-induced lsc Response --- p.79 / Chapter 3.2.4 --- Involvement of Adenylate Cyclase/cAMP/Protein Kinase A Pathway in Cordycepin-induced lsc Response --- p.84 / Chapter 3.2.5 --- Involvement of Ca2+-Dependent Pathway in Cordycepin-induced lsc Response --- p.89 / Chapter 3.2.6 --- Effect of Cordycepin on Intracellular Ca2+ Concentrations --- p.93 / Chapter 3.2.7 --- "Effect of Cordycepin on Apical CI"" Current and Basolateral K+ Current in Nystatin-Permeabilized Epithelia" --- p.98 / Chapter 3.2.8 --- Effect of Cordycepin on PKA Activity --- p.104 / Chapter Chapter 4 - --- Discussion / Chapter 4.1 --- Regulation of Ion Transport in 16HBE14o- Cells by CM Extract --- p.107 / Chapter 4.2 --- Intracellular Signaling Mechanisms behind CM-induced lsc Responses --- p.110 / Chapter 4.3 --- Regulation of Ion Transport in 16HBE14o- Cells by Cordycepin --- p.111 / Chapter 4.4 --- Intracellular Signaling Mechanisms behind Cordycepin-induced lsc Responses --- p.114 / Chapter Chapter 5 - --- Conclusion / Chapter 5.1 --- Summary --- p.117 / Chapter 5.2 --- Future Directions --- p.120 / Chapter Chapter 6 - --- References --- p.121 / Chapter Chapter 7 - --- Publications --- p.136