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No / The threat of biological weapons has never attracted as much public attention as in the past five years. Current concerns largely relate to the threat of weapons acquisition and use by rogue states or by terrorists. But the threat has deeper roots--it has been evident for fifty years that biological agents could be used to cause mass casualties and large-scale economic damage. Yet there has been little historical analysis of such weapons over the past half-century. Deadly Cultures sets out to fill this gap by analyzing the historical developments since 1945 and addressing three central issues: Why have states continued or begun programs for acquiring biological weapons? Why have states terminated biological weapons programs? How have states demonstrated that they have truly terminated their biological weapons programs? We now live in a world in which the basic knowledge needed to develop biological weapons is more widely available than ever before. Deadly Cultures provides the lessons from history that we urgently need in order to strengthen the long-standing prohibition of biological weapons

The use of biological agents as potential weapons has been a concern of security agencies for many years. Security agencies require alternative field protocols for handling forensic samples that could be contaminated with biological weapons. In this study, manual and automated DNA extractions were compared for the ability to remove biological agents and for their effectiveness and consistency when samples were contaminated with bacteria, spores or toxins. Purified DNA was evaluated for the absence of the agents, and for the effects of the process on the isolated human DNA. Results demonstrated that incubation of samples in a cell lysis solution eliminated bacteria and toxins, but an additional 0.22 µm filtration step was necessary to successfully remove bacterial spores. Blood and buccal swab samples exposed to some bacteria showed DNA loss and/or degradation. The automated extraction procedure would be preferable over the manual protocol to isolate human DNA contaminated with biological weapons.

The use of biological agents as potential weapons has been a concern of security agencies for many years. Security agencies require alternative field protocols for handling forensic samples that could be contaminated with biological weapons. In this study, manual and automated DNA extractions were compared for the ability to remove biological agents and for their effectiveness and consistency when samples were contaminated with bacteria, spores or toxins. Purified DNA was evaluated for the absence of the agents, and for the effects of the process on the isolated human DNA. Results demonstrated that incubation of samples in a cell lysis solution eliminated bacteria and toxins, but an additional 0.22 µm filtration step was necessary to successfully remove bacterial spores. Blood and buccal swab samples exposed to some bacteria showed DNA loss and/or degradation. The automated extraction procedure would be preferable over the manual protocol to isolate human DNA contaminated with biological weapons.

The use of biological agents as potential weapons has been a concern of security agencies for many years. Security agencies require alternative field protocols for handling forensic samples that could be contaminated with biological weapons. In this study, manual and automated DNA extractions were compared for the ability to remove biological agents and for their effectiveness and consistency when samples were contaminated with bacteria, spores or toxins. Purified DNA was evaluated for the absence of the agents, and for the effects of the process on the isolated human DNA. Results demonstrated that incubation of samples in a cell lysis solution eliminated bacteria and toxins, but an additional 0.22 µm filtration step was necessary to successfully remove bacterial spores. Blood and buccal swab samples exposed to some bacteria showed DNA loss and/or degradation. The automated extraction procedure would be preferable over the manual protocol to isolate human DNA contaminated with biological weapons.

The threat of biological weapons has never attracted as much public attention as in the past five years. Current concerns largely relate to the threat of weapons acquisition and use by rogue states or by terrorists. But the threat has deeper roots--it has been evident for fifty years that biological agents could be used to cause mass casualties and large-scale economic damage. Yet there has been little historical analysis of such weapons over the past half-century. "Deadly Cultures" sets out to fill this gap by analyzing the historical developments since 1945 and addressing three central issues: Why have states continued or begun programs for acquiring biological weapons? Why have states terminated biological weapons programs? How have states demonstrated that they have truly terminated their biological weapons programs? We now live in a world in which the basic knowledge needed to develop biological weapons is more widely available than ever before. "Deadly Cultures" provides the lessons from history that we urgently need in order to strengthen the long-standing prohibition of biological weapons.

Thesis (Ph.D.)--George Mason University, 2008. / Vita: p. 337. Thesis director: Christopher J. Davis. Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biodefense. Title from PDF t.p. (viewed Mar. 19, 2009). Includes bibliographical references (p. 308-336). Also issued in print.

Thesis (Ph. D., Microbiology, Molecular Biology and Biochemistry)--University of Idaho, October 2008. / Major professor: Scott A. Minnich. Includes bibliographical references. Also available online (PDF file) by subscription or by purchasing the individual file.

The use of biological agents as potential weapons has been a concern of security agencies for many years. Security agencies require alternative field protocols for handling forensic samples that could be contaminated with biological weapons. In this study, manual and automated DNA extractions were compared for the ability to remove biological agents and for their effectiveness and consistency when samples were contaminated with bacteria, spores or toxins. Purified DNA was evaluated for the absence of the agents, and for the effects of the process on the isolated human DNA. Results demonstrated that incubation of samples in a cell lysis solution eliminated bacteria and toxins, but an additional 0.22 µm filtration step was necessary to successfully remove bacterial spores. Blood and buccal swab samples exposed to some bacteria showed DNA loss and/or degradation. The automated extraction procedure would be preferable over the manual protocol to isolate human DNA contaminated with biological weapons.

The latest report Mapping the Global Future on US national security by the National Intelligence Council suggests that a major threat to the country right through to 2020 will be a terrorist biological weapons attack.1 Given the recent intelligence failures concerning biological weapons in Iraq, it might be considered that there are reasonable grounds for suspicion about that conclusion. This paper attempts to answer the question of what the real threat of bioterror is by reference to the open scientific literature. Section 2 of the paper discusses the nature of the agents of concern and in section 3 various potential attack scenarios are reviewed. The overall conclusion is that there are real threats from terrorists with the capability to carry out a range of attacks with biological agents today, but that these threats do not include the one most commentators probably have in mind when they discuss the issue ¿ a weapons of mass destruction scale of attack on people. In the final section of this paper the implications of the analysis for the risk questions we have been posed are addressed.