Yes / MXenes (viz., transition metal carbides, carbonitrides, and nitrides) have emerged as a new subclass of 2D materials. Due to their outstanding physicochemical and biological properties, MXenes have gained much attention in the biomedical field in recent years, including drug delivery systems, regenerative medicine, and biosensing. Additionally, the incorporation of MXenes into hydrogels has garnered significant interest in biomedical engineering as an electroactive and mechanical nanoreinforcer capable of converting nonconductive scaffolds into excellent conductors of electricity with an impressive effect on mechanical properties for the engineering of electroactive organs and tissues such as cardiac, skeletal muscle, and nerve. However, many questions and problems remain unresolved that need to be answered to usher these 2D materials toward their true destiny. Thus, this review paper aims to provide an overview of the design and applications of MXene-integrated composites for biomedical applications, including cardiac tissue engineering, wound healing, infection therapy, cancer therapy, and biosensors. Moreover, the current challenges and limitations of utilizing MXenes in vivo are highlighted and discussed, followed by its prospects as a guideline toward possible various futuristic biomedical applications. This review article will inspire researchers, who search for properties, opportunities, and challenges of using this 2D nanomaterial in biomedical applications. / Open Access Funding provided by Istituto Italiano di Tecnologia within the CRUI-CARE Agreement.

Soil microorganisms are important for the stabilization and preservation of a good soil structure. Management practices can affect the diversity and population of microorganisms, which could beneficially change soil properties and promote a more sustainable dryland system. This study was established near Pontotoc, MS on Atwood silt loam to evaluate the impacts of cover crops and fertilizer sources on selected biologically related soil properties in a no-tillage, dryland soybean system. Soil analyses included total carbon and nitrogen, permanganate oxidizable carbon (POXC), easily extractable glomalin-related soil protein (EE-GRSP), water stable aggregate (WSA) and soil pH. Cover crop biomass and percent groundcover, soybean leaf area index (LAI), plant height, and yield were also determined. Results indicated that fertilizer source did have an impact on total nitrogen, EE-GRSP and soybean yield. A positive impact on soil properties is expected with the use of cover crops if studied for a longer period of time.

<p>&nbsp / </p> <p align="left"><font face="Times New Roman">Aspalathus linearis (A. linearis), </font><font face="Times New Roman">commonly known as Rooibos tea or Red bush tea and </font><i><font face="Times New Roman">amellia sinensis (C. sinensis) </font></i><font face="Times New Roman">or Black tea are beverages that are consumed throughout the</font><font face="Times New Roman">world. These teas possess antioxidant, immunomodulating and anti-cancer actions. The aim</font> <font face="Times New Roman">of this study was to use <i>in vitro </i>assays to screen Rooibos and Black tea for endocrine</font> <font face="Times New Roman">modulation. </font><font face="Times New Roman">The immune modulating effects of Rooibos and Black tea were investigated using an <i>in vitro</i></font> <font face="Times New Roman">whole blood culture (WBC) assay. Unstimulated WBCs treated with Rooibos tea secreted</font> <font face="Times New Roman">higher levels of IL-6, IL-10 and IFN<font lang="JA">&gamma / </font>than cultures treated with DMSO control. Rooibos treatment of stimulated WBCs resulted in higher IL-6, lower IL-10 and no effect on IFN&gamma / </font><font face="Times New Roman">secretion compared to DMSO treated stimulated WBC. Black tea treatment of stimulated</font> <font face="Times New Roman">WBC resulted in decreased IL-6, IL-10 and IFN<font lang="JA">&gamma / </font>secretion compared to the DMSO treated</font> <font face="Times New Roman">stimulated WBC. </font><font face="Times New Roman">Extracts of Rooibos and Black tea were assessed for phytoestrogens using quantitative </font><font face="Times New Roman">estrogen ELISAs. Both teas contain phytoestrogens. The quantitative ELISAs showed that </font><font face="Times New Roman">Rooibos tea contained significantly lower estrone (E1), estradiol (E2) and estriol (E3) levels </font><font face="Times New Roman">than Black tea. The effects of Rooibos and Black tea on proliferation of the estrogen</font> <font face="Times New Roman">dependant MCF-7 cell line was determined to further characterise the phytoestrogenic</font> <font face="Times New Roman">properties of the teas. Both Rooibos and Black tea extracts caused a significant inhibition of</font> <font face="Times New Roman">MCF-7 proliferation. </font><font face="Times New Roman">This study shows that Rooibos tea and Black tea are beverages that can either stimulate or </font><font face="Times New Roman">suppress the immune system. Also, both teas contain significant levels of phytoestrogens as </font><font face="Times New Roman">determined by quantitative ELISAs. The current study confirms previous reports showing </font><font face="Times New Roman">inhibition of growth in breast cancer cell lines by phytoestrogens. The findings extend </font><font face="Times New Roman">related observations on the anti-carcinogenic potential of the two teas.</font></p>

In this study, it was aimed to investigate the structural, mechanical and biological properties of nano hydroxyapatite (HA) doped with yttrium and fluoride with different compositions. HAs were synthesized by precipitation method. After sintering at 900oC, 1100oC or 1300oC for 1 hour, the structural properties of HAs were investigated by XRD, FTIR spectroscopy and SEM. High relative densities (above 88 % of relative density) were achieved after sintering. No second phases were observed in XRD measurements. Hexagonal lattice parameters and unit cell volumes of doped HAs decreased indicating the substitutions of ions. Characteristics absorbtion bands of HA and additional bands due to fluoride substitutions were observed in FTIR patterns. SEM images showed that grain sizes decreased with increasing doping amounts and decreasing sintering temperatures. Discs prepared by cold pressing were sintered at 900oC, 1100oC and 1300oC for 1 hour to determine mechanical properties. Mechanical properties of HAs were found to be directly related to the sintering temperatures and amount of dopings. Biocompatibility of pure and doped HA discs was assessed with in vitro cytotoxicity studies. Cell attachment, proliferation and differentiation state of cells were studied using MTT, ALP and calcium assays and SEM. Cell attachment and proliferation were enhanced with dopings and increasing sintering temperatures. The highest ALP production and calcium deposition were observed on HAs sintered at 1100oC. In vitro studies revealed that 1100oC was the sintering temperature for best cell responses. Specifically, 2.5YFHA seemed to be promising as an alternative for pure HA among all doped HAs.

<p>&nbsp / </p> <p align="left"><font face="Times New Roman">Aspalathus linearis (A. linearis), </font><font face="Times New Roman">commonly known as Rooibos tea or Red bush tea and </font><i><font face="Times New Roman">amellia sinensis (C. sinensis) </font></i><font face="Times New Roman">or Black tea are beverages that are consumed throughout the</font><font face="Times New Roman">world. These teas possess antioxidant, immunomodulating and anti-cancer actions. The aim</font> <font face="Times New Roman">of this study was to use <i>in vitro </i>assays to screen Rooibos and Black tea for endocrine</font> <font face="Times New Roman">modulation. </font><font face="Times New Roman">The immune modulating effects of Rooibos and Black tea were investigated using an <i>in vitro</i></font> <font face="Times New Roman">whole blood culture (WBC) assay. Unstimulated WBCs treated with Rooibos tea secreted</font> <font face="Times New Roman">higher levels of IL-6, IL-10 and IFN<font lang="JA">&gamma / </font>than cultures treated with DMSO control. Rooibos treatment of stimulated WBCs resulted in higher IL-6, lower IL-10 and no effect on IFN&gamma / </font><font face="Times New Roman">secretion compared to DMSO treated stimulated WBC. Black tea treatment of stimulated</font> <font face="Times New Roman">WBC resulted in decreased IL-6, IL-10 and IFN<font lang="JA">&gamma / </font>secretion compared to the DMSO treated</font> <font face="Times New Roman">stimulated WBC. </font><font face="Times New Roman">Extracts of Rooibos and Black tea were assessed for phytoestrogens using quantitative </font><font face="Times New Roman">estrogen ELISAs. Both teas contain phytoestrogens. The quantitative ELISAs showed that </font><font face="Times New Roman">Rooibos tea contained significantly lower estrone (E1), estradiol (E2) and estriol (E3) levels </font><font face="Times New Roman">than Black tea. The effects of Rooibos and Black tea on proliferation of the estrogen</font> <font face="Times New Roman">dependant MCF-7 cell line was determined to further characterise the phytoestrogenic</font> <font face="Times New Roman">properties of the teas. Both Rooibos and Black tea extracts caused a significant inhibition of</font> <font face="Times New Roman">MCF-7 proliferation. </font><font face="Times New Roman">This study shows that Rooibos tea and Black tea are beverages that can either stimulate or </font><font face="Times New Roman">suppress the immune system. Also, both teas contain significant levels of phytoestrogens as </font><font face="Times New Roman">determined by quantitative ELISAs. The current study confirms previous reports showing </font><font face="Times New Roman">inhibition of growth in breast cancer cell lines by phytoestrogens. The findings extend </font><font face="Times New Roman">related observations on the anti-carcinogenic potential of the two teas.</font></p>

Scopo di questa tesi è stato di valutare se le attività biologiche possono essere utilizzate come indicatori di adattamento microbico. Le prove condotte sinora in letteratura si sono concentrate unicamente sull’attività di nitrificazione. Pertanto si è voluto valutare se altre attività biologiche possono essere utilizzate come indicatori d’adattamento. Allo scopo si è prima valutata la sensibilità di alcune importanti proprietà biologiche del suolo (nitrification, fluorescein diacetate (FDA)) e attività enzimatiche (urease, nitrate reductase, phosphatase, arylsulfatase, β-galactosidase, phenol-oxidase and dehydrogenase) allo Zn mediante l’impiego di test ecotossicologici Per le attività più sensibili sono state condotte delle prove di adattamento secondo il protocollo di Rusk. Questo esclude le interferenze dovuto all’ aging. Questo protocollo è basato sul reinoculo di un suolo sterile (contaminato con concentrazioni crescenti di Zn) con sottocampioni di suolo che sono stati precedentemente incubati per un periodo di 4 mesi con e senza Zn Il confronto tra i valori di EC50 delle attività biologiche dei suoli reinoculati ci ha permesso di dimostrare un significativo recupero della β-galactosidase, mentre per il nitrate reductase e la nitrificazione potenziale è stato trovato un chiaro e significativo shift delle curve di dose e risposta, anche se con parziale sovrapposizione del range dell’EC50 stimato. / The purpose of this thesis was to assess whether the biological activities can be used as indicators of the microbial adaptation. The tests conducted so far in the literature have focused only nitrification activity. Therefore we wanted to assess whether other biological activities can be used as indicators of adaptation. To do this you first evaluated the sensitivity of some important biological properties of the soil (nitrification, fluorescein diacetate (FDA)) and enzymatic activities (urease, nitrate reductase, phosphatase, arylsulfatase, β-galactosidase, phenol-oxidase and dehydrogenase) Zn through the use of toxicological test. Then the activities most sensitive were tests to evaluate to adapt according to the protocol by Rusk This method to exclude interferences to two chemical aging in soil, This protocol is based on the reinoculation of sterilized soil (contaminated with increasing Zn concentrations) with sub-samples of soil which have been incubated for 4 months with or without Zn. The comparison between the EC50 of the biological properties of reinoculated soils allow us to demonstrate a significant restoration was found for β-galactosidase, while for nitrate reductase and potential nitrification there was a clear and significant shift of dose response curves but with partial overlap of the EC50 ranges estimation.

Magister Scientiae - M.Sc. / Aspalathus linearis (A. linearis), commonly known as Rooibos tea or Red bush tea and Camellia sinensis (C. sinensis) or Black tea are beverages that are consumed throughout the world. These teas possess antioxidant, immunomodulating and anti-cancer actions. The aim of this study was to use in vitro assays to screen Rooibos and Black tea for endocrine modulation. The immune modulating effects of Rooibos and Black tea were investigated using an in vitro whole blood culture (WBC) assay. Unstimulated WBCs treated with Rooibos tea secreted higher levels of IL-6, IL-10 and IFNγ than cultures treated with DMSO control. Rooibos treatment of stimulated WBCs resulted in higher IL-6, lower IL-10 and no effect on IFNγ secretion compared to DMSO treated stimulated WBC. Black tea treatment of stimulated WBC resulted in decreased IL-6, IL-10 and IFNγ secretion compared to the DMSO treated stimulated WBC. Extracts of Rooibos and Black tea were assessed for phytoestrogens using quantitative estrogen ELISAs. Both teas contain phytoestrogens. The quantitative ELISAs showed that Rooibos tea contained significantly lower estrone (E1), estradiol (E2) and estriol (E3) levels than Black tea. The effects of Rooibos and Black tea on proliferation of the estrogen dependant MCF-7 cell line was determined to further characterise the phytoestrogenic properties of the teas. Both Rooibos and Black tea extracts caused a significant inhibition of MCF-7 proliferation. This study shows that Rooibos tea and Black tea are beverages that can either stimulate or suppress the immune system. Also, both teas contain significant levels of phytoestrogens as determined by quantitative ELISAs. The current study confirms previous reports showing inhibition of growth in breast cancer cell lines by phytoestrogens. The findings extend related observations on the anti-carcinogenic potential of the two teas. / South Africa

Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-07-13T14:09:49Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Zenaide S Monte - Tese Doutorado - Defesa 15-jul-2016.pdf: 10509605 bytes, checksum: 9a625f96607c0ccae6d58b896ba38772 (MD5) / Made available in DSpace on 2017-07-13T14:09:49Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Zenaide S Monte - Tese Doutorado - Defesa 15-jul-2016.pdf: 10509605 bytes, checksum: 9a625f96607c0ccae6d58b896ba38772 (MD5) Previous issue date: 2016-07-15 / CAPES / Compostos heterocíclicos do tipo pirimidina são conhecidos pela enorme riqueza de seus potenciais biológicos e farmacológicos. Numerosas reações de heterociclização que permitem a obtenção dessa classe de compostos são igualmente reconhecidas, devido à diversidade de atividades e do conhecimento prévio de inúmeras viabilidades sintéticas. As modificações estruturais obtidas na busca da otimização das rotas sintéticas conferem às novas moléculas diferentes propriedades físicas e alteram a reatividade das moléculas, acarretando em mudanças na distribuição nas células e nos tecidos. Essas discretas modificações estruturais podem revelar efeitos biológicos que haviam estado latentes ou eclipsados pelos efeitos colaterais do composto protótipo. Sendo assim, o presente trabalho apresenta a síntese por método convencional dos derivados da pirimidina 3a-q e 5o-q. Estes compostos foram avaliados quanto às propriedades duais: Antimicrobiana e Fotoluminescência, estas apresentaram resultados promissores, podem atuar como boas sondas fluorescentes. Foram realizados também estudos Espectrofotométricos em diferentes solventes além de determinarmos o Cálculo do Orbital Molecular de Fronteira nesta série. Realizou-se também a nanoencapsulação em lipossomas para o composto 3o. Obtivemos também p-arilamidinas 3a-j por método convencional e avaliamos as propriedades Antimicrobiana e Citotóxica, as quais apresentaram resultados significativos. Realizamos a síntese dos derivados pirimidínicos 3a-f e 6a-f pelo método em Irradiação em micro-ondas, em fase sólida e em solução, respectivamente. Estudamos também para estes as propriedades Antimicrobiana e Antioxidante, cujos os resultados foram satisfatórios. Foram obtidos bons rendimentos para todos os compostos obtidos. Um estudo inédito de RMN bidimensional, COSY, HSQC e HMBC foram aplicados às pirimidinas foi realizado, proporcionando a caracterização estrutural destes compostos. Os resultados obtidos apresentaram significativa potencialidades biológicas, farmacológicas e fotofísicas. / Heterocyclic compounds of pyrimidine are known for rich biological and pharmacological potential. A number of heterociclic reactions that enable the obtention of this class of compounds are also recognized, due to the diversity of activities and prior knowledge of their various synthetic viabilities. The structural modifications obtained in search for the best synthetic routes confered different physical properties on the new molecules and altered the reactivity of the molecules, resulting in changes in the distribution in cells and tissues. These discrete structural changes may reveal biological effects that may have been latent or eclipsed by the side effects of the compound prototype. This work presents the synthesis by conventional method of derivatives from pyrimidine 3a-q and 5o-q. These compounds were evaluated for dual properties: antimicrobial and photoluminescence. They showed promising results can act as fluorescent probes. Spectroscopic studies were carried out with different solvents and the Frontier Molecular Orbital calculation for this series was determined. Nanoencapsulation of liposomes for the compound 3o was prepared. The conventional method was used to obtain 3a-j p-arilamidinas. Results were significant for the antimicrobial and cytotoxic properties. Finally, the synthesis of pyrimidines derivatives 3a-f and 6a-f by a new method, solid phase micro-wave and in solution, respectively was carried out. We have studied both the Antimicrobial and Antioxidant properties with satisfactory results. Good yields were obtained for all compounds obtained. New studies of two-dimensional NMR, COSY, HSQC and HMBC were performed besides the characterization of the compounds by standard methods. The results show that the compounds obtained have great biological, pharmacological potential and Photophysical.

O solo é um importante sumidouro de carbono (C) atmosférico, uma vez que concentrações de CO2 da atmosfera podem ser atenuadas através de mecanismos de sequestro de C no solo. Nesse contexto, solos sob clima semi-árido estão sendo atualmente avaliados como potenciais sequestradores de C, sobretudo no processo de recuperação de áreas degradadas. No entanto, ainda há carência de informações referentes aos mecanismos envolvidos no sequestro de C. Adicionalmente, há incertezas nas estimativas dos estoques de C pela falta de conhecimento sobre sua variabilidade espacial devido à complexidade dos processos físicos, químicos e biológicos que influenciam o ciclo do referido elemento. As variações espaciais do C no solo estão relacionadas a fatores naturais e induzidos pelo homem e essas variações apresentam-se em diferentes escalas espaciais. Por tais motivos a presente pesquisa teve como objetivo avaliar a variabilidade espacial do C e de outros atributos físicos, químicos e biológicos do solo numa área de 100 ha destinada ao reflorestamento na região semi-árida do Rio Grande do Norte. Para tanto, foi estabelecida uma grade regular de 644 pontos amostrais espaçados de 40 m numa área localizada no município de Angicos (RN), pertencente à Universidade Federal Rural do SemiÁrido. Foram coletadas 1932 amostras de solo nas profundidades 0-0,1; 0,1-0,2; e 0,2-0,3 m para determinações de C, areia, silte, argila, pH, Na, P, Ca, Mg e K. Para determinar o C da biomassa microbiana (Cmic) foram utilizadas 156 amostras referentes à camada 0-0,1m. Com relação à densidade do solo (Ds) foram coletadas 246 amostras nas três profundidades mencionadas anteriormente. Adicionalmente, foram efetuados os seguintes cálculos: estoques de C, saturação por bases (V %), CTC (CTC(T)), saturação por Al (m) e soma de bases (SB). Os resultados foram submetidos à análise estatística descritiva clássica, seguida de análise geoestatística. O solo da área apresenta predominância de textura muito arenosa, baixa CTC e teores de C e Cmic; altos valores de Ds, acidez elevada e médios teores P e bases disponíveis. Estes resultados são comuns em solos de região semi-árida sob Caatinga em decorrência das condições climáticas desfavoráveis. Na análise descritiva a maioria dos atributos avaliados apresentou normalidade na sua distribuição. Os coeficientes de variação (CV) foram classificados como médios para a maioria dos atributos, a dependência espacial foi moderada com média geral dos alcances de 135 m. A maioria dos atributos ajustou-se ao modelo esférico. Na avaliação da eficiência dos modelos ajustados, tanto a validação interna como a externa apresentaram comportamentos semelhantes. A modelagem aplicada permitiu estimar o alcance e a magnitude das dependências espaciais. Por meio da krigagem foi efetuada a interpolação dos dados e gerados os mapas de variabilidade espacial para os atributos físicos, químicos e biológicos estudados. Os resultados obtidos nesta pesquisa ressaltam a importância do entendimento da variabilidade espacial do C e outras propriedades do solo, informações que servem como ponto de referência inicial (linha de base) e tem implicações importantes para futuras avaliações do impacto no sequestro de C e do potencial produtivo de Jatrofa na região semi-árida do Nordeste Brasileiro. / Soil is an important carbon (C) sink, since atmospheric CO2 concentrations can be attenuated by soil C sequestration. In this context, soils under semi-arid conditions are being evaluated as potential soil C sinks, mainly considering the process of rehabilitation of degraded areas. However, little information is available on the mechanisms associated with soil C sequestration. Moreover, there are uncertainties on soil C stocks estimates because of the lack of knowledge about its spatial variability due to the complexity of physical, chemical and biological processes that directly influence soil C cycle. Soil C spatial variability is associated with a series of natural and human-induced factors and those variations can be expressed in different spatial scales. Therefore, the main objective of the present study was to evaluate the spatial variability of C and soil physical, chemical and biological attributes in a 100 ha area destined to reforestation in the semi-arid region of Rio Grande do Norte (RN), Brazil. In order to do that, a regular grid (40 x 40 m) of 644 sampling points was defined in an area located in the city of Angicos (RN) that belongs to the Universidade Federal Rural do Semi-Árido. Samples from the 0-0,1; 0,1-0,2; e 0,2- 0,3 m soil layers were collected in each sampling point totalizing 1932 soil cores that were used for the following analyses: C, sand, silt, clay, pH, Na, P, Ca, Mg and K. For microbial biomass C, 156 samples were used from the 0-0,1 m soil depth. Samples for soil bulk density (total of 246 samples) were collected in the three mentioned soil layers. Additionally, the following calculations were performed: soil C stocks, base saturations, cation exchange capacity and aluminum saturation. The results were analyzed using classical descriptive statistics and geostatistics. The soil at the studied area is very sandy and presented low values of cation exchange capacity, C content and microbial biomass; high values of bulk density and soil acidity and medium values of P content and available bases. Those results are typical for soils under native vegetation at the semi-arid region due to unfavorable climatic conditions. From the descriptive analyses, the majority of the studied attributes presented normal distribution. The coefficients of variation (CV) for the majority of the studied attributes presented medium values; the spatial dependence was moderated with mean range value of 135 m. The majority of the attributes were fitted by the spherical model. Assessment of model adjustment efficiency was performed through internal and external validations and both presented similar trends. The application of modeling technique provides estimations of the range and the spatial dependence magnitude of the evaluated soil attributes. Using kriging techniques, analytical results were interpolated and maps were generated to show the spatial variability of the soil physical, chemical and biological properties. The results from the present study stressed the importance of adequately understand C and other soil properties spatial variability. Such information has important implications for future assessments of soil C sequestration and is useful for potential production of Jatropha in the semi-arid condition of the Brazilian northeast region.

O solo é um importante sumidouro de carbono (C) atmosférico, uma vez que concentrações de CO2 da atmosfera podem ser atenuadas através de mecanismos de sequestro de C no solo. Nesse contexto, solos sob clima semi-árido estão sendo atualmente avaliados como potenciais sequestradores de C, sobretudo no processo de recuperação de áreas degradadas. No entanto, ainda há carência de informações referentes aos mecanismos envolvidos no sequestro de C. Adicionalmente, há incertezas nas estimativas dos estoques de C pela falta de conhecimento sobre sua variabilidade espacial devido à complexidade dos processos físicos, químicos e biológicos que influenciam o ciclo do referido elemento. As variações espaciais do C no solo estão relacionadas a fatores naturais e induzidos pelo homem e essas variações apresentam-se em diferentes escalas espaciais. Por tais motivos a presente pesquisa teve como objetivo avaliar a variabilidade espacial do C e de outros atributos físicos, químicos e biológicos do solo numa área de 100 ha destinada ao reflorestamento na região semi-árida do Rio Grande do Norte. Para tanto, foi estabelecida uma grade regular de 644 pontos amostrais espaçados de 40 m numa área localizada no município de Angicos (RN), pertencente à Universidade Federal Rural do SemiÁrido. Foram coletadas 1932 amostras de solo nas profundidades 0-0,1; 0,1-0,2; e 0,2-0,3 m para determinações de C, areia, silte, argila, pH, Na, P, Ca, Mg e K. Para determinar o C da biomassa microbiana (Cmic) foram utilizadas 156 amostras referentes à camada 0-0,1m. Com relação à densidade do solo (Ds) foram coletadas 246 amostras nas três profundidades mencionadas anteriormente. Adicionalmente, foram efetuados os seguintes cálculos: estoques de C, saturação por bases (V %), CTC (CTC(T)), saturação por Al (m) e soma de bases (SB). Os resultados foram submetidos à análise estatística descritiva clássica, seguida de análise geoestatística. O solo da área apresenta predominância de textura muito arenosa, baixa CTC e teores de C e Cmic; altos valores de Ds, acidez elevada e médios teores P e bases disponíveis. Estes resultados são comuns em solos de região semi-árida sob Caatinga em decorrência das condições climáticas desfavoráveis. Na análise descritiva a maioria dos atributos avaliados apresentou normalidade na sua distribuição. Os coeficientes de variação (CV) foram classificados como médios para a maioria dos atributos, a dependência espacial foi moderada com média geral dos alcances de 135 m. A maioria dos atributos ajustou-se ao modelo esférico. Na avaliação da eficiência dos modelos ajustados, tanto a validação interna como a externa apresentaram comportamentos semelhantes. A modelagem aplicada permitiu estimar o alcance e a magnitude das dependências espaciais. Por meio da krigagem foi efetuada a interpolação dos dados e gerados os mapas de variabilidade espacial para os atributos físicos, químicos e biológicos estudados. Os resultados obtidos nesta pesquisa ressaltam a importância do entendimento da variabilidade espacial do C e outras propriedades do solo, informações que servem como ponto de referência inicial (linha de base) e tem implicações importantes para futuras avaliações do impacto no sequestro de C e do potencial produtivo de Jatrofa na região semi-árida do Nordeste Brasileiro. / Soil is an important carbon (C) sink, since atmospheric CO2 concentrations can be attenuated by soil C sequestration. In this context, soils under semi-arid conditions are being evaluated as potential soil C sinks, mainly considering the process of rehabilitation of degraded areas. However, little information is available on the mechanisms associated with soil C sequestration. Moreover, there are uncertainties on soil C stocks estimates because of the lack of knowledge about its spatial variability due to the complexity of physical, chemical and biological processes that directly influence soil C cycle. Soil C spatial variability is associated with a series of natural and human-induced factors and those variations can be expressed in different spatial scales. Therefore, the main objective of the present study was to evaluate the spatial variability of C and soil physical, chemical and biological attributes in a 100 ha area destined to reforestation in the semi-arid region of Rio Grande do Norte (RN), Brazil. In order to do that, a regular grid (40 x 40 m) of 644 sampling points was defined in an area located in the city of Angicos (RN) that belongs to the Universidade Federal Rural do Semi-Árido. Samples from the 0-0,1; 0,1-0,2; e 0,2- 0,3 m soil layers were collected in each sampling point totalizing 1932 soil cores that were used for the following analyses: C, sand, silt, clay, pH, Na, P, Ca, Mg and K. For microbial biomass C, 156 samples were used from the 0-0,1 m soil depth. Samples for soil bulk density (total of 246 samples) were collected in the three mentioned soil layers. Additionally, the following calculations were performed: soil C stocks, base saturations, cation exchange capacity and aluminum saturation. The results were analyzed using classical descriptive statistics and geostatistics. The soil at the studied area is very sandy and presented low values of cation exchange capacity, C content and microbial biomass; high values of bulk density and soil acidity and medium values of P content and available bases. Those results are typical for soils under native vegetation at the semi-arid region due to unfavorable climatic conditions. From the descriptive analyses, the majority of the studied attributes presented normal distribution. The coefficients of variation (CV) for the majority of the studied attributes presented medium values; the spatial dependence was moderated with mean range value of 135 m. The majority of the attributes were fitted by the spherical model. Assessment of model adjustment efficiency was performed through internal and external validations and both presented similar trends. The application of modeling technique provides estimations of the range and the spatial dependence magnitude of the evaluated soil attributes. Using kriging techniques, analytical results were interpolated and maps were generated to show the spatial variability of the soil physical, chemical and biological properties. The results from the present study stressed the importance of adequately understand C and other soil properties spatial variability. Such information has important implications for future assessments of soil C sequestration and is useful for potential production of Jatropha in the semi-arid condition of the Brazilian northeast region.