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Efeito do tratamento t?rmico do tit?nio sobre a prolifera??o de c?lulas pr?-osteobl?sticasMacedo, Haroldo Reis Alves de 20 August 2008 (has links)
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Previous issue date: 2008-08-20 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Titanium is a biomaterial widely employed in biomedical applications (implants, prostheses, valves, stents). Several heat treatments are usually used in order to obtain physical properties required to different applications. This work studied the influence of the heat treatment on microstructure of commercial pure titanium, and their consequences in growth and proliferation of MC3T3-E1 cells. Discs of titanium were treated in different temperatures, and characterized by optical microscopy, image analysis, wettabillity, roughness, hardness and X-ray diffraction. After the heat treatment, significant modifications in these properties were observed. Pattern images of titanium, before and after the cell culture, were compared by overlapping to analyze the influence of microstructure in microstructure and preferences guidance cells. However, in general, titanium discs that showed a higher residual strength also presented an increase of cells numbers on surface / O tit?nio como biomaterial ? amplamente utilizado em dispositivos biom?dicos (implantes, pr?teses, v?lvulas, stents entre outros). Diversos tratamentos t?rmicos s?o usualmente utilizados na obten??o das propriedades necess?rias para as diferentes aplica??es. Este trabalho estudou a influ?ncia desses tratamentos na microestrutura do tit?nio comercialmente puro e suas conseq??ncias no crescimento, forma e prolifera??o de c?lulas pr?-osteobl?stica. Para tanto foram utilizados discos de tit?nio submetidos a diferentes tratamentos e caracterizados por microscopia ?tica, an?lise de imagens, molhabilidade, rugosidade, dureza e difra??o de raios-X. Ap?s os tratamentos t?rmicos foram verificado modifica??es significantes nestas propriedades. Padr?es de imagens de superf?cies do tit?nio antes e ap?s a cultura de c?lula foram comparados atrav?s de sobreposi??o para analisar a influ?ncia da microestrutura na resposta biol?gica, n?o sendo verificadas correla??es entre a microestrutura e as prefer?ncias orientacionais das c?lulas. Entretanto de um modo geral verificou-se que os discos que presentaram maior estado de tens?o residual apresentaram tamb?m maior n?mero de c?lulas em sua superf?cie
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