Summary Appropriate, timely, and effective control of sterilisation ranks among the most relevant objectives and aims of a personal healthcare institution. Implementation and evaluation of sterilisation control is one of the ways to ensure quality and safety of medicine. The aim of the study was to evaluate the effectiveness of the sterilisation process using biological indicators, and to verify the implementation of the biological control of sterilizers. The methods of the study. The retrospective analysis of the data of biological control of sterilisation performed in 2003 and 2004. The study evaluated the performance of biological control of the effectiveness of 81 (2003) saturated steam and 76 (2004) dry heat sterilizers. The obtained findings were evaluated according to the requirements of 2003 and 2004 set by the legislation and the internal control of the institution. Results was analysed using Epi info, MS Excel, SPSS statistical programs. Results. In 2003, the number of performed biological inspection of sterilisation was by 1.37 times lesser than that indicated in the legislation. In 2004, this number increased by 1.56 times, and by 1.27 times exceeded the frequency of biological control indicated in the legislation. In 2003, the mean frequency of biological control for one sterilizer was 5.43 cases per year, and in 2004 – 8.82 cases per year. During 2003, correct (meeting all requirements) biological control was performed in 16 (19.75%) sterilizers, and in 2004... [to full text]