District heating is an efficient way to integrate renewable energies in the energy mix. In the French region Ile-de-France, district heating and renewables have known strong developments for several years, and they are expected to grow much more in the future. The expansion of renewable energies depends partly on their competitiveness compared to fossil fuels, which is related to public subsidies, and on the spread of district heating networks in the region. Thus, it could be assess what is the future business model for district heating based on renewables in Ile-de-France. The data at the disposal of ADEME, the French agency for environment and renewable energies, show that district heating systems based on biomass could soon become more competitive than fossil fuels, whereas systems based on geothermal heat have fairly high costs. Yet, geothermal heat is more developed than biomass, and is expected to reach the targets set by the regional action plan for 2020, on the contrary to biomass. Though the increasing competitiveness of renewables will cut the necessity of public subsidies, the difficulty to reach some regional targets might mean that further actions should be implemented. From the energy operators’ point of view, the sector of district heating will surely grow considerably and therefore represent a large potential of development for those companies.

Thesis (M.Sc. (Microbiology)) -- University of Limpopo, 2020 / Production of bioethanol from lignocellulosic biomass has gained significant attention worldwide as an alternative fuel source for the transportation sector without affecting food supply. Efficient conversion of pentose sugars (L-arabinose and D-xylose) produced during hydrolysis of hemicellulose to ethanol can enhance the economic viability. In this study, a total of 390 yeasts isolated from Marula wine, the gut of dung beetles, herbal concoctions and banana residues were screened for the ability to ferment L-arabinose and D-xylose. Fourteen yeasts were able to ferment both pentose sugars and ten strains were subjected to an adaptation process in the presence of acetic acid using L-arabinose as carbon source. Four adapted strains of Meyerozyma caribbica were able to ferment L-arabinose to ethanol and arabitol in the presence of 3 g/L acetic acid at 35 °C. Meyerozyma caribbica Mu 2.2f fermented D-xylose, L-arabinose and a mixture of D-xylose and L-arabinose to produce 1.7, 3.0 and 1.9 g/L ethanol, respectively, compared to the parental strain with 1.5, 1.0 and 1.8 g/L ethanol, respectively, in the absence of acetic acid. The adapted strain of M. caribbica Mu 2.2f produced 3.6 and 0.8 g/L ethanol from L-arabinose and D-xylose, respectively in the presence of acetic acid while the parental strain failed to grow. In the bioreactor, the adapted strain of M. caribbica Mu 2.2f produced 5.7 g/L ethanol in the presence of 3 g/L acetic acid with an ethanol yield and productivity of 0.338 g/g and 0.158 g/L/h, respectively at a KLa value of 3.3 h-1. The adapted strain produced 26.7 g/L arabitol with a yield of 0.900 g/g at a KLa value of 4.9 h-1. Meyerozyma caribbica Mu 2.2f could potentially be used to produce ethanol and arabitol under stressed conditions. / National Research Foundation (NRF)

Für den Anbau von Biomasse-Dauerkulturen wie schnellwachsende Baumarten werden Anbauhinweise und -empfehlungen gegeben, die Aspekte des Boden-, Gewässer- und Naturschutzes berücksichtigen. Standorteignung, Wirkungen auf wildlebende Pflanzen- und Tierarten, entstehende Synergieeffekte, Einflüsse auf den Wasserhaushalt, Stoffeinträge in Gewässer sowie klimatische Wirkungen sind Teilaspekte, unter denen die Chancen und Risiken der energetischen Nutzung nachwachsender Rohstoffe im Rahmen eines mehrjährigen Verbundvorhabens in Sachsen untersucht wurden. Ergänzend wurde auch der Anbau einjähriger Energiepflanzen betrachtet.

Thesis (Ph.D. (Microbiology)) -- University of Limpopo, 2021 / Biofuel production from lignocellulose material is an attractive alternative to fossil fuel. The use of lignocellulose material for biofuel production is imperative because of the numerous advantages that it offers. Biofuel is environmentally friendly and in developing countries such as South Africa, it has the potential to reduce the use of imported fuel and create jobs. Currently, several constraints are affecting the implementation of biofuel. One of the constraints is the cost-effectiveness and the efficiency of the enzymes involved in the enzymatic degradation of lignocellulose polymers to monomers, which can further be fermented to bioethanol. The potential way to reduce enzymatic degradation cost could be by supplementing the fungal enzymes with accessory enzymes such as endo-xylanase. The enzyme production cost is also dependent on the carbon source used. Lignocellulose materials that are regarded as waste must be assed for their use as enzyme inducer carbon sources and as biomass for biofuel production. This is a potential route that will reduce enzyme and biofuel production costs. Biofuel production cost can further be reduced by finding a yeast that can ferment xylose and ferment in the presence of inhibitors released during lignocellulose pretreatment. This study sought to tackle the enzymatic hydrolysis constraints and also search for xylose-fermenting yeast by exploring the gut microbiota of dung beetle. The gut of the dung beetle has recently received great attention since it is proposed to be a bioreactor for lignocellulolytic microorganisms that can be used in biofuel applications. This is because dung beetles feed on the dung of herbivorous animals and the dung is composed of 80% undigested plant material. In this study the guts of four Scarabaeidae dung beetles Kheper nigroanaeus Boheman, Heteronitis castelnaui, Pachylomerus femoralis, Anachalcos convexus and dung beetle larvae, Euoniticellus intermedius were screened for hemicellulolytic fungi and xylose-fermenting yeast. Hundred and thirty-two yeast isolates and two-hundred and twenty-two filamentous fungi were isolated and identified using ITS and D1/D2 regions. The yeast isolates were assigned to 8 genera and 18 species, Trichosporon was the most dominant genus while Candida tropicalis was the most dominant specie. Some of the yeast isolates were identified as uncultured fungi. This yeast must be characterised to be certain if they are novel species. The fungal isolates were assigned to 12 genera and 25 species, Aspergillus was the most dominant genus while Hypocrea lixii was the most dominant specie. The yeast isolated could assimilate xylose and could grow at a maximum temperature of 40 °C. Furthermore, these yeast isolates could also grow in the presence of 3 g/L acetic acid. Most of the fungal isolates had xylanolytic activity. The phylogenetic analysis revealed close genetic relatedness between isolates from the different dung beetle species and dung beetle larvae. The profile of the fungal genera was similar in the different dung beetles. Both guts and the larvae had Aspergillus, Hypocrea, Trichoderma, Talaromyces and Penicillium. The filamentous fungi that showed good xylanolytic activity were further screened for their ability to produce xylanase enzyme using thatch grass as an inductive carbon source. Thatch grass was selected in this study since it is in-house plant-based biomass. Thatch grass is abundantly available in South Africa; it is used for animal grazing but the more it grows it loses its nutritional content. Once it reaches this stage, it is no longer used and most of it is burnt. The fire from burning grass contains higher levels of nitrogen-containing chemicals that pollute the environment. Its compositional analysis (cellulose 46%, hemicellulose 27% and lignin 10%) also attributed to its selection as potential inductive carbon and attractive lignocellulose biomass for biofuel production. The higher xylanase activity of 283.43, 270 and 287.03 nkat/ml were observed from Aspergillus fumigatus L1XYL9 (Euoniticellus intermedius larvae), Hypocrea lixii AB2A3 and Neosartotya sp AB2XYL20 (Anachalcos convexus), respectively. This was achieved when acid pretreated thatch grass was used as an inductive carbon source. Aspergillus fumigatus L1XYL9 (Euoniticellus intermedius larvae), Hypocrea lixii AB2A3 and Neosartotya sp AB2XYL20 (Anachalcos convexus) showed xylanase activity of 393,22, 313,06 and 200 nkat/ml when grown on synthetic xylan. Neosartotya sp AB2XYL20 showed higher xylanase activity on thatch grass. The suitable production process for xylanase enzyme on acid pretreated thatch grass was assessed by conducting a comparative study on solid-state and submerged fermentation using L1XYL9 (Euoniticellus intermedius larvae), Hypocrea lixii AB2A3 and Neosartotya sp AB2XYL20 (Anachalcos convexus) as the best xylanase producer on acid pretreated thatch grass. The strain showed better xylanase activity when submerged fermentation was used. In this study, Hypocrea lixii AB2A3 was selected for further studies since it was the most dominant species and also showed good xylanase activity. Thatch grass was pretreated differently to evaluate the suitable chemical for pretreating thatch grass. Thatch grass was pretreated with dilute sulphuric acid 1.2% and maintained the pH of 5.5 by using sodium hydroxide while another batch was pretreated the same way and was washed with distilled water till pH of 5.5. The other batch was then pretreated with ammonium solution and was also washed with distilled water to maintain a pH of 5.5. The above-mentioned pretreated thatch grass was tested as an inductive carbon source as well as untreated thatch grass. The xylanase activity was determined to assess a good inductive carbon. All the thatch grass pretreated and washed with distilled water showed very low xylanase activity. The untreated thatch grass resulted in lower xylanase activity as compared to xylanase activity achieved when pretreated thatch grass was used. Parameters such as agitation speed and initial inoculum size were also assessed during xylanase production by Hypocrea lixii AB2A3 on acid pretreated thatch grass. Xylanase activity increased from 525 nkat/ml (Inoculum size 2×106 spore/ml and agitation speed 150 rpm) to 584.8 nkat/ml (Inoculum size 2×106 spore/ml and agitation speed 200 rpm). The crude xylanase from Hypocrea lixii AB2A3 was used to hydrolyse acid pretreated thatch grass. This resultant in xylose yield of 138 mg/g of substrate and glucose yield of 49 mg/g of substrate. Crude xylanase was mixed with commercial celluclast™. This enzyme mixture resulted in a xylose yield of 128 mg/g substrate and a glucose yield of 549 mg/g of substrate. The results obtained in this study show that indeed gut of the dung beetles and dung beetle larvae are a rich source of microorganisms that can play an important role in biofuel application and remediating the environment by degrading plant-based biomass regarded as waste into valuable products. It is imperative to evaluate the gut microbiota of dung beetles from different regions in South Africa for their application in the biofuel industry to reinforce its implementation. Thatch grass is a potential inductive carbon and lignocellulose biomass for biofuel production. / NRF (National Research Foundation)

Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Nutrition and Food Science, 1980. / MICROFICHE COPY AVAILABLE IN ARCHIVES AND ENGINEERING. / Bibliography: leaves 201-212. / Rajen Kantilal Dalal. / M.S.

PhD thesis deals with application of simulation and optimization methods in the field of waste and biomass utilization for energy purposes. Current situation in this field is described in the introductory. Following chapters deal with approaches of creating mathematical models of apparatus and processes used in the field of interest for simulation and optimization purposes. Stochastic methods, which are widely applied for real problems solution, are mentioned as well. The core of the thesis consists in proposal of systematic approach and its application for simulation and optimization model building used in the field of interest. The application is demonstrated through two case studies. The first one deals with the building of simulation model of an existing waste-to-energy plant using its operation data. The second study deals with optimization model building and its application for a problem regarding utilization of biomass in an existing energy system.

Thesis (M.A. (Health Sociology))--University of the Witwatersrand, Faculty of Humanities, 2016. / This study examines use of different energy sources by a poor community of the Ramaphosa Informal Settlement in Gauteng Province, South Africa. The purpose of this study was to investigate the reasons behind continued use of biomass fuel (plant or animal material, wood, charcoal) for cooking and space heating by poor residents. The research questions are: What informs the informal settlement residents’ use of certain energy sources for cooking and heating over other types? Where residents possess knowledge of the harmful effects of continued use of an energy source, yet continue to use it, what are the reasons for this? Whose responsibility does it become to collect a chosen energy source, and how is it collected? The consequences of indoor air pollution vary from short-term – eye and throat irritation – to long-term effects – respiratory disease and cancer. Exposure to high levels of some pollutants, such as carbon monoxide, can even result in immediate death. An exploratory empirical research was performed using mixed qualitative and quantitative methods using data on time-activity patterns collected from eleven households by means of semi-structured interviews, observations, focus group discussions and expert interviews. The results show that the respondents in the researched areas of Reserve and Extension two in Ramaphosa Informal Settlement use a total of thirteen different energy sources to meet their fuel needs. Although possessing the necessary knowledge on negative effects of indoor air pollution, the respondents lack sufficient resources to make decisions that would help improve their conditions regarding effects of air pollution. In thirty of the fifty respondents women and girls collect fuel and only in the remaining twenty wherein electricity, paraffin and liquid petroleum gas (LPG) are used, do men and boys become responsible for fuel collection. In the absence of electricity, respondents reported preferences for LPG, however, the prohibitive costs of the capital outlay of the latter energy source makes it unaffordable to more than half of the respondents. The major finding in this report is that whilst some of the respondents think that electricity remains a key barrier to improving their socio-economic development and well-being, twenty of the fifty respondents who exclusively rely on government grants do not think so. Electricity, although an absolute necessity in the researched areas, is not a sufficient condition for avoidance of effects of indoor air pollution for the poor communities. This was demonstrated by the five respondents who have electricity but alternate its use with coal and firewood. The high cost of electricity means that poorer communities will continue to rely on the less expensive bio-mass fuel – risking their lives in the process – even when electricity is available. Respondents reported difficult conditions under which they live which are shaped by broader sets of unresolved structural aspects in the form of economics, social policies, and politics. / GR 2017

M.Tech. (Department of Chemical Engineering, Faculty of Engineering and Technology), Vaal University of Technology. / An enormous amount of food waste (FW) is generated worldwide. Most of this waste is discarded in landfills, where it undergoes uncontrolled anaerobic digestion (AD) process, which emits excessive amounts of greenhouse gases, (methane and carbon dioxide), thereby contributing to global warming. A controlled AD of FW is key for organic waste management with a positive impact on the environment and economy. In South Africa (SA) there is little uptake of biogas technology for FW management due to little research on biogas potential at small to large scale. Furthermore, there is an over reliance on foreign data, which leads to misfit parameters to local raw materials; consequently, producing biogas of low quality and quantity with low degradation of waste. Biogas with poor quality reduces the efficiency of biogas conversion to energy and the low production rate makes the system less feasible. Considering the challenges faced with FW management and the little uptake of the AD technology in SA, this study aimed to treat FW through AD and convert the biogas produced to electricity. A complete-mix biogas pilot plant (VUT-1000C) was designed, constructed and commissioned. The materials used for constructing the pilot plant were sourced locally to prove the applicability of the AD technology in SA. The biodigester was operated at mesophilic temperature, 37 oC, aided by a solar system. A stand-alone 1 m3 plug-flow ambient biodigester (STH-1000A) was operated semi-continuously as well as a control. Cow dung (CD) was used to inoculate the biodigesters, which were then operated semi-continuously at their optimum organic loading rate (OLR). The STH-1000A digester was operated at 0.446 kgVS/m3/day OLR, according to the manufacturer’s specification, while for VUT-1000C, the OLR was determined. The highest biogas and methane yields obtained were 582 and 332 L/kgVS/m3, respectively, at the determined optimal OLR of 1.5 kgVS/m3/day for the VUT-1000C digester this was supported by the modified Gompertz model with an R2 value of 0.9836. VUT-1000C produced 1200 L/day while STH-1000A produced 150 L/day. VUT-1000C proved to be a more effective biodigester than STH-1000A owing to the digester design and operation at mesophilic conditions. The key design findings are higher reactor working volume and high digester temperature. From the 1000 L of biogas produced from VUT-1000C, 1.8 kW of electricity was generated, which is equivalent to powering 300 6W light bulbs for 1 hour. The energy balance of the pilot plant showed that only 10 percent of the energy output was required to operate the plant. These results show that SA has a 475 GWh energy potential based on the current FW figures. Furthermore, the study has shown that biogas technology is readily available for South Africans and that the designed biogas plant was very efficient in FW-to-energy conversion.

No description available.

The use of biomass as a renewable fuel source can help the United States reduce its dependence on fossil fuels, especially in providing affordable heat for many middle- and low-income households. However, residential wood combustion (RWC) releases pollutants that can negatively impact the environment and human health, especially for those living in the vicinity of wood-burning locations. Current compliance testing methods are insufficient in capturing the actual in-use emissions of residential wood heaters because they do not represent real-life use, leading to higher emissions during actual use. This thesis investigates emissions during realistic operations of wood-fired heaters to identify and quantify the majority of emissions and ways to minimize such emissions. The study focuses on investigating eight different woody biomass fired heaters, including three pellet stoves, a pellet boiler, two wood chip-fired hydronic heaters, and two outdoor cordwood fired hydronic heaters. This research contributes a new knowledge on the impact of combustion strategies, fuel type, and control strategies to minimize emissions. The obtained data can provide information to manufacturers, policy makers, and consumers, guiding low-emission and more efficient use of wood-fired heating devices. In all chapters, variability was evident due to burn phase, fuel type, and operation. The results from the pellet stoves showed that even while using a homogeneous fuel, different burn phases produce different emissions than the overall period. For the pellet boiler studies, the highest efficiencies were achieved during the high load, steady state tests. The burn phase also affects emissions from woodchip boilers, where low output periods are significantly higher in terms of emissions compared to high output periods. Each individual burn phase of the duty cycle produced different emissions in cordwood testing, with steady-state phases having the lowest emissions and highest efficiency. The variability in emissions from different burn phases is a crucial factor in evaluating the performance of wood-burning appliances. Lower moisture content fuels were found to have better performance in terms of PM emissions and efficiency. Fuel type can impact emissions, but it may be overshadowed by burn phase and technology. Relatively high emissions were often related to low or incorrect air-to-fuel ratios. Gasification techniques used in some woodchip boilers during low output periods significantly increased efficiency and reduced CO emissions. Additionally, gasification techniques used during high burn steady states with wet fuel chips resulted in a 77% reduction in PM emissions. Comparing all the primary heaters studied in this thesis, in terms of PM emission output, showed the units that used gasification, integrated catalysts, or thermal storage had the lowest emissions. The results of the study are compiled into data sets that give a more accurate picture of real-world operation of wood-fired heaters that will benefit air quality modelers and policy makers. Such emission data for various biomass heaters in EPA’s AP-42, under realistic operating conditions, currently either does not exist or is limited. Additionally, this research identifies the most important parameters that need to be included in the development of high-resolution models, optimizing the performance of wood-fired devices and supporting the transition from current compliance testing to more realistic testing. In conclusion, this work provides new insights into the performance and emissions of wood-fired heaters during realistic operation. The results of the study can help manufacturers optimize their products for real-life performance and help policy makers and consumers make informed decisions regarding low-emission and more efficient use of wood-fired heating devices. The study highlights the importance of capturing transient phases and the impact of fuel type and control strategies on minimizing emissions.