Made available in DSpace on 2016-04-28T20:18:34Z (GMT). No. of bitstreams: 1 Caroline Rodrigues Pereira Martins.pdf: 1134388 bytes, checksum: fa8d41a81a1364e1de4a2a59b34737ed (MD5) Previous issue date: 2009-10-29 / The use of bovine pericardium as a biological membrane is widespread in surgical repair because of the low cost, good applicability and it is easily obtained. Glycerin 98% is the chemical agent most used as a biological tissue preservative in veterinary medicine, but studies show it is ineffective in eliminating viruses. Glutaraldehyde is a biological tissue preservative widely used in 0.5% and 0.625% concentrations. However, the persistence of rabies virus in samples of mice pericardium preserved in 0.625% glutaraldehyde was proven, and probably higher concentrations are required for virucidal action. Given its toxic capacity, this study aims to evaluate macro-and microscopically kidney, liver and spleen of 65 mice, 60 of whom underwent implantation of bovine pericardium preserved in glycerin 98% and 0.625%, 1% and 1.5% glutaraldehyde, and 5 non-implanted mice served as controls. The animals implanted, the same used in other research to evaluate local reaction on the abdominal wall implants, were euthanized after 7, 14 and 30 days after implantation of the pericardium, and the spleens, livers and kidneys were assessed as to their macro and microscopic features. Fragments of these organs were collected, fixed in buffered formalin 10%, processed by usual histopathologic methods and stained with hematoxylin and eosin. There was no macro or microscopic changes that could be related to the toxicity of these substances / O emprego de peric?rdio bovino, como membrana biol?gica, ? bastante difundido em t?cnicas de reparos cir?rgicos devido ? facilidade de aquisi??o, baixo custo e boa aplicabilidade. A glicerina 98% ? o agente qu?mico mais utilizado como conservante de tecidos biol?gicos em medicina veterin?ria, por?m estudos comprovam sua inefic?cia na elimina??o viral. O glutaralde?do ? um conservante de tecidos biol?gicos e suas concentra??es mais difundidas s?o de 0,5% e 0,625%. Entretanto, a persist?ncia do v?rus r?bico em amostras de peric?rdio de camundongos conservadas em glutaralde?do 0,625% foi comprovada, sendo, provavelmente, necess?rias concentra??es mais altas para a??o virucida. Sabendo-se da sua capacidade t?xica, este estudo teve como objetivo, avaliar macro e microscopicamente rins, f?gado e ba?o de 65 camundongos, dos quais 60 foram submetidos a implante de peric?rdio bovino conservado em glicerina 98% e glutaralde?do a 0,625%, 1% e 1,5% e 5 camundongos n?o implantados que serviram como controles. Os animais implantados, os mesmos utilizados em outra pesquisa para avalia??o das caracter?sticas reacionais locais nas paredes abdominais aos implantes, foram eutanasiados ap?s 7, 14 e 30 dias ap?s a implanta??o do peric?rdio; e os ba?os, f?gados e rins foram avaliados quanto ?s caracter?sticas macro e microsc?picas. Fragmentos desses ?rg?os foram coletados, fixados em formol a 10% tamponados, processados pelos m?todos usuais para histopatologia e corados pela hematoxilina e eosina. N?o foram evidenciadas altera??es macro ou microsc?picas que pudessem estar relacionadas ? toxicidade destas subst?ncias.