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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Effects of selected emulsion components; (bovine serum albumin, lipase and glutaraldehyde), on the surface properties of Polydimethylsiloxane.

Windvoel, Victoria Thobile January 2011 (has links)
Thesis (MSc (Biochemistry))--University of Limpopo, 2011. / The purpose of this study was to investigate the effects of bovine serum albumin (BSA), lipase and glutaraldehyde on the surface of polydimethylsiloxane (PDMS). PDMS blocks of 15 by 15mm were fabricated using replica micromolding. To determine the effects of BSA, clean PDMS blocks were immersed in 20, 50 and 100mg/ml BSA, respectively. For the effect of lipase, the concentration was 20, 50 and 70mg/ml. To determine the effect of glutaraldehyde, 5, 10, 20, 30, 40 and 50% concentration was used. All the PDMS blocks were immersed for 10, 20 and 30 minutes. The water contact angle was measured on all the PDMS surfaces, including a clean surface as a control. This analysis was done using a drop shape analyzer, DSA 100 machine. The PDMS surfaces were further analyzed by Fourier Transform InfraRed (FTIR spectroscopy), using a Pelkin-Elmer FT-IR spectrometer. PDMS blocks which were pre-immersed in 5% glutaraldehyde and then in BSA and lipase solutions, respectively, were also added for FTIR analysis. The water surface tension was measured for both BSA and lipase and interfacial tension was measured for glutaraldehyde, using the DSA 100. The results indicated that the water contact angle decreases after the PDMS surface has been immersed in all the solutions prepared. FTIR analysis showed new peaks on the PDMS surface immersed in BSA, and in BSA and glutaraldehyde; however, there were no peaks formed on the PDMS surface immersed in lipase and washed, in glutaraldehyde, and in lipase and glutaraldehyde together. Surface tension measurements showed that BSA and lipase decreases the surface tension of water. Interfacial tension measurements also showed that glutaraldehyde decreases the interfacial tension of oil. BSA, lipase and glutaraldehyde therefore decrease the hydrophobicity of the PDMS surface. BSA adsorbs on the PDMS surface and the adsorption is irreversible. The adsorption of lipase on the PDMS surface is reversible. Glutaraldehyde does not adsorb on the surface or the adsorption is not detectable. BSA, lipase and glutaraldehyde all have surface active properties. The CMC value of BSA is 50mg/ml and of lipase is 15mg/ml.
2

A comparative study of the effects of glutaraldehyde and formocresol on the skin of young, adult and aged rats a dissertation [sic] submitted in partial fulfillment ... pedodontics ... /

Vazquez-Rivera, Humberto. January 1982 (has links)
Thesis (M.S.)--University of Michigan, 1982.
3

Immunomodulatory roles of endotoxin and glutaraldehyde in the development of latex allergy

Howell, Michael D. January 1900 (has links)
Thesis (Ph. D.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains xvii, 194 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 163-181).
4

A comparative study of the effects of glutaraldehyde and formocresol on the skin of young, adult and aged rats a dissertation [sic] submitted in partial fulfillment ... pedodontics ... /

Vazquez-Rivera, Humberto. January 1982 (has links)
Thesis (M.S.)--University of Michigan, 1982.
5

AvaliaÃÃo da biocompatibilidade e bioatividade de membranas colÃgeno polianiÃnico mineralizadas e reticuladas em modelos animais. / Evaluation of biocompatibility and bioactivity of collagen polyanionic membranes mineralized and crosslinked in animal models.

Denusa Moreira VerÃssimo 09 March 2012 (has links)
nÃo hà / O objetivo desse estudo foi avaliar membranas de colÃgeno polianiÃnico (CPA) reticuladas e impregnadas com hidroxiapatita, manufaturadas pelo Departamento de FÃsica da Universidade Federal do CearÃ. Dividiu-se o trabalho em 2 etapas, onde inicialmente avaliou-se a biocompatibilidade e a biodegradaÃÃo de 6 diferentes membranas de CPA, divididas nos seguintes grupos: trÃs com 0, 25 e 75 ciclos de impregnaÃÃo com hidroxiapatita (CPA, CPA 25, CPA 75) e mais trÃs cujas membranas foram reticuladas com glutaraldeÃdo (GA) (CPA GA, CPA 25GA, CPA 75GA) inseridas em tecido subcutÃneo de ratos. AnÃlises histopatolÃgicas do infiltrado inflamatÃrio, atividade de mieloperoxidase (MPO), dosagem de citocinas, espessura de cÃpsula fibrosa, imunohistoquÃmica para metaloproteinase e biodegradaÃÃo das membranas foram avaliadas apÃs 1, 7, 15, 30, 60 e 120 dias. Posteriormente, avaliou-se o efeito das 3 melhores membranas na regeneraÃÃo Ãssea guiada usando defeito Ãsseo crÃtico em calvÃria de ratos (DOC), onde as membranas foram posicionadas sobre o defeito. FormaÃÃo Ãssea foi avaliada com base na radiografia digital (RD), tomografia computadorizada (TC) e anÃlise histolÃgica, 24 horas, 4, 8 e 12 semanas apÃs o procedimento cirÃrgico. MPO e dosagem de citocinas foram realizadas apÃs 24 horas. No subcutÃneo, as membranas reticuladas com GA mostraram espessa cÃpsula fibrosa e menor reaÃÃo inflamatÃria permanecendo intactas apÃs 120 dias. No modelo de regeneraÃÃo Ãssea em calvÃria de ratos, apÃs 12 semanas, os grupos CPA GA e CPA 25GA apresentaram reduÃÃo significativa da Ãrea radiolÃcida quando comparadas ao grupo basal. A anÃlise histolÃgica mostrou que nos grupos CPA GA e CPA 25GA as membranas ainda estavam intactas, envolvidas por uma espessa cÃpsula fibrosa e as membranas do grupo CPA 75GA apresentaram inÃcio de reabsorÃÃo. NÃo foi encontrada diferenÃa estatÃstica entre os grupos quanto a atividade MPO e citocina IL-1β. ConcluÃmos que as membranas reticuladas mostraram-se mais biocompatÃveis e se mantiveram livre de biodegradaÃÃo no perÃodo de observaÃÃo. Essas membranas induziram o fechamento dos defeitos Ãsseos e nÃo induziram reaÃÃo inflamatÃria. A impregnaÃÃo de hidroxiapatita nÃo acelerou a cicatrizaÃÃo do defeito cirÃrgico. Nossos resultados sugerem que as membranas de CPA reticuladas poderÃo ser Ãteis nos processos em que a formaÃÃo de um novo osso depende de uma duraÃÃo mais prolongada da barreira mecÃnica. / The aim of this study was to evaluate polyanionic collagen (PAC) membranes reticulated and impregnated with hydroxyapatite, manufactured by the Physics Department, Federal University of CearÃ. This study was divided into two stages, initially it was evaluated the biocompatibility and biodegradation of six different membranes of PAC, divided into the following groups: three with 0, 25 and 75 cycles of impregnation with apatite (PAC, PAC 25, PAC 75 ) and three more whose membranes were crosslinked with glutaraldehyde (GA) (GA PAC, PAC 25GA, PAC 75GA) inserted into the subcutaneous tissue of rats. Histopathological analyzes of inflammatory infiltration, myeloperoxidase activity (MPO), cytokine, thickness of fibrous capsule, immunohistochemistry for metalloproteinase and degradation of the membranes were evaluated after 1, 7, 15, 30, 60 and 120 days. Subsequently, it was evaluated the effect of the three best membranes in guided bone regeneration using bone critical defects in rat calvaria (DOC), where the membranes were placed over the defect. Bone formation was evaluated based on digital radiography (DR), computed tomography (CT) and histological analysis, 24 hours, 4, 8 and 12 weeks after surgery. MPO and cytokine were performed after 24 hours. In the subcutaneous tissue, the membranes crosslinked with GA showed thick fibrous capsule, less inflammatory reaction and remained intact after 120 days. In the bone regeneration model in rat calvaria, after 12 weeks, PAC GA and PAC 25GA groups showed significant reduction in radiolucent area compared to the baseline group. Histological analysis showed that in PAC GA and PAC 25GA groups, membranes were still intact, surrounded by a thick fibrous capsule and in PAC 75GA group, membranes showed early resorption. There was no statistical difference between groups in MPO activity and IL-1β. We conclude that the crosslinked membranes were more biocompatible and remained free from degradation during the observation period. These membranes induced closure of bone defects and did not induce inflammatory reaction. The impregnation of hydroxyapatite did not accelerate the healing of surgical defect. Our results suggest that the crosslinked membranes CPA may be useful in cases where new bone formation is dependent on a longer duration of mechanical barrier.
6

Avalia??o anatomopatologica de rins, f?gado e ba?o de camundongos submetidos ao reparo de ferida cir?rgica em parede abdominal com fragmentos de peric?rdio bovino conservado com glicerina e glutaralde?do. / Histopathological evaluation of kidneys, liver and spleen of mice undergoing repair of surgical wound in the abdominal wall with pieces bovine pericardium preserved with glycerin and glutaraldehyde

Martins, Caroline Rodrigues Pereira 29 October 2009 (has links)
Made available in DSpace on 2016-04-28T20:18:34Z (GMT). No. of bitstreams: 1 Caroline Rodrigues Pereira Martins.pdf: 1134388 bytes, checksum: fa8d41a81a1364e1de4a2a59b34737ed (MD5) Previous issue date: 2009-10-29 / The use of bovine pericardium as a biological membrane is widespread in surgical repair because of the low cost, good applicability and it is easily obtained. Glycerin 98% is the chemical agent most used as a biological tissue preservative in veterinary medicine, but studies show it is ineffective in eliminating viruses. Glutaraldehyde is a biological tissue preservative widely used in 0.5% and 0.625% concentrations. However, the persistence of rabies virus in samples of mice pericardium preserved in 0.625% glutaraldehyde was proven, and probably higher concentrations are required for virucidal action. Given its toxic capacity, this study aims to evaluate macro-and microscopically kidney, liver and spleen of 65 mice, 60 of whom underwent implantation of bovine pericardium preserved in glycerin 98% and 0.625%, 1% and 1.5% glutaraldehyde, and 5 non-implanted mice served as controls. The animals implanted, the same used in other research to evaluate local reaction on the abdominal wall implants, were euthanized after 7, 14 and 30 days after implantation of the pericardium, and the spleens, livers and kidneys were assessed as to their macro and microscopic features. Fragments of these organs were collected, fixed in buffered formalin 10%, processed by usual histopathologic methods and stained with hematoxylin and eosin. There was no macro or microscopic changes that could be related to the toxicity of these substances / O emprego de peric?rdio bovino, como membrana biol?gica, ? bastante difundido em t?cnicas de reparos cir?rgicos devido ? facilidade de aquisi??o, baixo custo e boa aplicabilidade. A glicerina 98% ? o agente qu?mico mais utilizado como conservante de tecidos biol?gicos em medicina veterin?ria, por?m estudos comprovam sua inefic?cia na elimina??o viral. O glutaralde?do ? um conservante de tecidos biol?gicos e suas concentra??es mais difundidas s?o de 0,5% e 0,625%. Entretanto, a persist?ncia do v?rus r?bico em amostras de peric?rdio de camundongos conservadas em glutaralde?do 0,625% foi comprovada, sendo, provavelmente, necess?rias concentra??es mais altas para a??o virucida. Sabendo-se da sua capacidade t?xica, este estudo teve como objetivo, avaliar macro e microscopicamente rins, f?gado e ba?o de 65 camundongos, dos quais 60 foram submetidos a implante de peric?rdio bovino conservado em glicerina 98% e glutaralde?do a 0,625%, 1% e 1,5% e 5 camundongos n?o implantados que serviram como controles. Os animais implantados, os mesmos utilizados em outra pesquisa para avalia??o das caracter?sticas reacionais locais nas paredes abdominais aos implantes, foram eutanasiados ap?s 7, 14 e 30 dias ap?s a implanta??o do peric?rdio; e os ba?os, f?gados e rins foram avaliados quanto ?s caracter?sticas macro e microsc?picas. Fragmentos desses ?rg?os foram coletados, fixados em formol a 10% tamponados, processados pelos m?todos usuais para histopatologia e corados pela hematoxilina e eosina. N?o foram evidenciadas altera??es macro ou microsc?picas que pudessem estar relacionadas ? toxicidade destas subst?ncias.
7

Effects of glutaraldehyde and formocresol on the connective tissue matrix of young, adult and aged rats a dissertation submitted in partial fulfillment ... pedodontics ... /

Doty, Raymond Kimble. January 1982 (has links)
Thesis (M.S.)--University of Michigan, 1982.
8

The effects of glutaraldehyde and diluted formocresol on rat connective tissue a histological and radioautographic study /

Arias-Garay, Francisco. Urquiola-Graham, Rebeca. January 1983 (has links)
Thesis (M.S.)--University of Michigan, 1983.
9

Effects of glutaraldehyde and formocresol on the connective tissue matrix of young, adult and aged rats a dissertation submitted in partial fulfillment ... pedodontics ... /

Doty, Raymond Kimble. January 1982 (has links)
Thesis (M.S.)--University of Michigan, 1982.
10

The effects of glutaraldehyde and diluted formocresol on rat connective tissue a histological and radioautographic study /

Arias-Garay, Francisco. Urquiola-Graham, Rebeca. January 1983 (has links)
Thesis (M.S.)--University of Michigan, 1983.

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