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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Studies on mitogen and co-mitogen from natural sources.

January 1988 (has links)
by Song Myung-eun. / Thesis (M.Ph.)--Chinese University of Hong Kong, 1988. / Bibliography: leaves 67-70.
2

Characterization of the sialic acid component in a bioactive extract from the edible bird's nest.

January 1991 (has links)
by Ng Ping-chung. / Thesis (M. Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references. / Chapter 1. --- Introduction / Chapter 1.1 --- Natural History of the Bird and the Nest --- p.1 / Chapter 1.2 --- What is More Important in Saliva: Mucin or Proteoglycan? --- p.9 / Chapter 1.3 --- Extraction and characterization of salivary glycoprotein --- p.27 / Chapter 2. --- Materials and Methods / Chapter 2.1 --- Preparation of Swiftlet's Nest Extracts --- p.38 / Chapter 2.2 --- Chemical and Biochemical Analysis of SN extracts --- p.38 / Chapter 2.2.1 --- Chemical Analysis --- p.38 / Chapter 2.2.1.1 --- Element Analysis --- p.38 / Chapter 2.2.1.2 --- Ash and Atomic Absorption Analysis --- p.39 / Chapter 2.2.2 --- Biochemical Analysis --- p.39 / Chapter 2.2.2.1 --- Protein Determination --- p.39 / Chapter 2.2.2.2 --- Hexose Determination --- p.40 / Chapter 2.2.2.3 --- Uronic Acid Determination --- p.40 / Chapter 2.2.2.4 --- Hexosamine Determination --- p.41 / Chapter 2.2.2.5 --- Sialic Acid Determination --- p.42 / Chapter 2.2.2.6 --- Sulphate Determination --- p.42 / Chapter 2.3 --- Assay of Co-mitogenic Activity in Lymphocyte Culture --- p.43 / Chapter 2.3.1 --- Preparation of Human Peripheral Blood Lymphocytes --- p.43 / Chapter 2.3.2 --- Co-mitogenic Assay --- p.43 / Chapter 2.4 --- Effect of SN pretreatment on Concanavalin A-stimulated Blastogenic Response in Mouse Splenocytes --- p.44 / Chapter 2.4.1 --- Administration of SN extracts --- p.44 / Chapter 2.4.2 --- Preparation of Mouse Splenocytes --- p.45 / Chapter 2.4.3 --- Concanavalin A-stimulated Blastogenic Response Assay --- p.45 / Chapter 2.5 --- Characterization of SN extracts by Chromatographic Methods --- p.45 / Chapter 2.5.1 --- Gel Filtration Chromatography --- p.45 / Chapter 2.5.1.1 --- Sephadex G-200 Chromatography --- p.45 / Chapter 2.5.1.2 --- Superose-Fast Protein Liquid Chromatography --- p.46 / Chapter 2.5.2 --- Ion-exchange Chromatography --- p.46 / Chapter 2.5.2.1 --- DEAE-Sepharose --- p.46 / Chapter 2.5.2.2 --- Mono-Q FPLC --- p.46 / Chapter 2.5.3 --- Immobilized Metal Affinity Chromatography --- p.47 / Chapter 2.5.4 --- Wheat-germ lectin Sepharose Chromatography --- p.47 / Chapter 2.5.5 --- Octyl-Sepharose Chromatography --- p.47 / Chapter 2.5.6 --- Limulus Polyemus Agarose Chromatography --- p.48 / Chapter 2.5.7 --- Heparin-Agarose Chromatography --- p.48 / Chapter 2.6 --- Electrophoretic Analysis of SN extract --- p.49 / Chapter 2.6.1 --- Sodium Dodecyl Sulphate/Polyacrylamide Gel Electrophoresis --- p.49 / Chapter 2.6.2 --- Isoelectrofocusing in Polyacrylamide Gel --- p.50 / Chapter 2.6.2.1 --- Preparation of Gel --- p.50 / Chapter 2.6.2.2 --- Isoelectrofocusing Procedure --- p.51 / Chapter 2.6.2.3 --- "Fixing, Staining and Destaining" --- p.51 / Chapter 2.6.3 --- Gradient Polyacrylamide Gel Electrophoresis --- p.51 / Chapter 2.6.3.1 --- Preparation of Gradient Polyacrylamide Gel --- p.52 / Chapter 2.7 --- Enzymatic Modification of SN extracts --- p.53 / Chapter 2.7.1 --- B-glucuronidase (EC 3.2.1.31) --- p.53 / Chapter 2.7.2 --- Hyaluronidase (EC 3.2.1.35) --- p.53 / Chapter 2.7.3 --- Chondroitinase ABC (EC 4.2.2.4) --- p.54 / Chapter 2.8 --- Miscellaneous Reagents --- p.54 / Chapter 2.8.1 --- Phosphate-buffer-saline (PBS) --- p.54 / Chapter 2.8.2 --- Fetal Calf Serum (FGS) --- p.54 / Chapter 2.8.3 --- Mitogen --- p.55 / Chapter 2.3.4 --- Penicillin-Streptomycin-Fungizone Solution --- p.55 / Chapter 2.8.5 --- RPMI-1640 Medium --- p.55 / Chapter 2.8.6 --- Scintillant --- p.55 / Chapter 2.8.7 --- Trypan Blue Solution --- p.55 / Chapter 3 --- Results --- p.57 / Chapter 3.1 --- Extraction of Biologically active fractions from swiflet's nest --- p.57 / Chapter 3.2 --- Effect of SN pretreatment on Con A-stimulated Blastogenesis response in mouse splenocytes --- p.57 / Chapter 3.3 --- Chemical and Biochemical Analysis of SN extracts --- p.57 / Chapter 3.3.1 --- Atomic absorption analysis of metal elements --- p.57 / Chapter 3.3.2 --- Element analysis --- p.61 / Chapter 3.3.3 --- Biochemical analysis --- p.61 / Chapter 3.4 --- Chromatographic characterization of SN extracts --- p.67 / Chapter 3.4.1 --- Sephadex G-200 chromatography --- p.67 / Chapter 3.4.2 --- DEAE-Sepharose chromatography --- p.67 / Chapter 3.4.3 --- Immobilized Metal Affinity Chromatography --- p.73 / Chapter 3.4.4 --- Wheat-germ lectin Sepharose Chromatography --- p.73 / Chapter 3.4.5 --- Limulus Polyemus Agarose Chromatography --- p.73 / Chapter 3.4.6 --- Octyl-Sepharose Chromatography --- p.81 / Chapter 3.4.7 --- Heparin-Agarose Chromatography --- p.81 / Chapter 3.5 --- Electrophoretic Analysis of SN extract --- p.88 / Chapter 3.5.1 --- Sodium Dodecyl Sulphate/Polyacrylamide Gel Electrophoresis --- p.88 / Chapter 3.5.2 --- Isoelectrofocusing in Thin Layer of Polyacrylamide Gel --- p.88 / Chapter 3.5.3 --- Gradient Polyacrylamide Gel Electrophoresis --- p.88 / Chapter 3.6 --- Enzymatic Modification of SN extracts --- p.92 / Chapter 3.6.1 --- B-glucuronidase (EC 3.2.1.31) --- p.92 / Chapter 3.6.1.1 --- Alteration in co-mitogenic activity --- p.92 / Chapter 3.6.1.2 --- Alternation in uronic acid content --- p.92 / Chapter 3.7 --- Co-mitogenic activity of glycosaminoglycans --- p.92 / Chapter 3.8 --- Effect of heparin on the co-mitogenic activity of SNp2C fraction --- p.96 / Chapter 4 --- Discussion --- p.99 / Chapter 4.1 --- Extraction of Biologically active fraction from edible bird's nest --- p.99 / Chapter 4.2 --- Chemical and Biochemical characterization of active component(s) in SN extract --- p.100 / Chapter 4.3 --- Some mechanistic considerations --- p.107 / Chapter 4.4 --- Summary and Conclusion --- p.111 / References --- p.114
3

Influence of brood-size manipulation on nestling growth, fledging success and parental behaviour in American Kestrels

Gard, Nicholas W. (Nicholas William), 1962- January 1989 (has links)
No description available.
4

Influence of brood-size manipulation on nestling growth, fledging success and parental behaviour in American Kestrels

Gard, Nicholas W. (Nicholas William), 1962- January 1989 (has links)
No description available.

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