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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Studies on mitogen and co-mitogen from natural sources.

January 1988 (has links)
by Song Myung-eun. / Thesis (M.Ph.)--Chinese University of Hong Kong, 1988. / Bibliography: leaves 67-70.
2

Interleukin 1 production in health and disease

Mitchell, Renee January 1981 (has links)
Being a Dissertation presented in fulfilment of the requirement governing the Degree of Master of Science in The School of Medicine, University of the Witwatersrand / Interleukin 1 (IL - 1) is a macrophage factor that exerts control over T cell activation. The experimental work presented in this dissertation consists of studies on IL - 1 production by monocytes which can be used as an in vitro correlate for monocyte function, as well as the effect of IL - 1 on immature T cells, that is, thymocytes. In accomplishing the studies presented in this dissertation, a two stage assay was employed. Firstly IL - 1 containing supernatants were produced by stimulated human peripheral blood adherent cells and secondly, the IL - 1 supernatants were assayed on mouse thymocyctes in which these supernatants caused mitogenesis. / IT2018
3

Characterization of the sialic acid component in a bioactive extract from the edible bird's nest.

January 1991 (has links)
by Ng Ping-chung. / Thesis (M. Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references. / Chapter 1. --- Introduction / Chapter 1.1 --- Natural History of the Bird and the Nest --- p.1 / Chapter 1.2 --- What is More Important in Saliva: Mucin or Proteoglycan? --- p.9 / Chapter 1.3 --- Extraction and characterization of salivary glycoprotein --- p.27 / Chapter 2. --- Materials and Methods / Chapter 2.1 --- Preparation of Swiftlet's Nest Extracts --- p.38 / Chapter 2.2 --- Chemical and Biochemical Analysis of SN extracts --- p.38 / Chapter 2.2.1 --- Chemical Analysis --- p.38 / Chapter 2.2.1.1 --- Element Analysis --- p.38 / Chapter 2.2.1.2 --- Ash and Atomic Absorption Analysis --- p.39 / Chapter 2.2.2 --- Biochemical Analysis --- p.39 / Chapter 2.2.2.1 --- Protein Determination --- p.39 / Chapter 2.2.2.2 --- Hexose Determination --- p.40 / Chapter 2.2.2.3 --- Uronic Acid Determination --- p.40 / Chapter 2.2.2.4 --- Hexosamine Determination --- p.41 / Chapter 2.2.2.5 --- Sialic Acid Determination --- p.42 / Chapter 2.2.2.6 --- Sulphate Determination --- p.42 / Chapter 2.3 --- Assay of Co-mitogenic Activity in Lymphocyte Culture --- p.43 / Chapter 2.3.1 --- Preparation of Human Peripheral Blood Lymphocytes --- p.43 / Chapter 2.3.2 --- Co-mitogenic Assay --- p.43 / Chapter 2.4 --- Effect of SN pretreatment on Concanavalin A-stimulated Blastogenic Response in Mouse Splenocytes --- p.44 / Chapter 2.4.1 --- Administration of SN extracts --- p.44 / Chapter 2.4.2 --- Preparation of Mouse Splenocytes --- p.45 / Chapter 2.4.3 --- Concanavalin A-stimulated Blastogenic Response Assay --- p.45 / Chapter 2.5 --- Characterization of SN extracts by Chromatographic Methods --- p.45 / Chapter 2.5.1 --- Gel Filtration Chromatography --- p.45 / Chapter 2.5.1.1 --- Sephadex G-200 Chromatography --- p.45 / Chapter 2.5.1.2 --- Superose-Fast Protein Liquid Chromatography --- p.46 / Chapter 2.5.2 --- Ion-exchange Chromatography --- p.46 / Chapter 2.5.2.1 --- DEAE-Sepharose --- p.46 / Chapter 2.5.2.2 --- Mono-Q FPLC --- p.46 / Chapter 2.5.3 --- Immobilized Metal Affinity Chromatography --- p.47 / Chapter 2.5.4 --- Wheat-germ lectin Sepharose Chromatography --- p.47 / Chapter 2.5.5 --- Octyl-Sepharose Chromatography --- p.47 / Chapter 2.5.6 --- Limulus Polyemus Agarose Chromatography --- p.48 / Chapter 2.5.7 --- Heparin-Agarose Chromatography --- p.48 / Chapter 2.6 --- Electrophoretic Analysis of SN extract --- p.49 / Chapter 2.6.1 --- Sodium Dodecyl Sulphate/Polyacrylamide Gel Electrophoresis --- p.49 / Chapter 2.6.2 --- Isoelectrofocusing in Polyacrylamide Gel --- p.50 / Chapter 2.6.2.1 --- Preparation of Gel --- p.50 / Chapter 2.6.2.2 --- Isoelectrofocusing Procedure --- p.51 / Chapter 2.6.2.3 --- "Fixing, Staining and Destaining" --- p.51 / Chapter 2.6.3 --- Gradient Polyacrylamide Gel Electrophoresis --- p.51 / Chapter 2.6.3.1 --- Preparation of Gradient Polyacrylamide Gel --- p.52 / Chapter 2.7 --- Enzymatic Modification of SN extracts --- p.53 / Chapter 2.7.1 --- B-glucuronidase (EC 3.2.1.31) --- p.53 / Chapter 2.7.2 --- Hyaluronidase (EC 3.2.1.35) --- p.53 / Chapter 2.7.3 --- Chondroitinase ABC (EC 4.2.2.4) --- p.54 / Chapter 2.8 --- Miscellaneous Reagents --- p.54 / Chapter 2.8.1 --- Phosphate-buffer-saline (PBS) --- p.54 / Chapter 2.8.2 --- Fetal Calf Serum (FGS) --- p.54 / Chapter 2.8.3 --- Mitogen --- p.55 / Chapter 2.3.4 --- Penicillin-Streptomycin-Fungizone Solution --- p.55 / Chapter 2.8.5 --- RPMI-1640 Medium --- p.55 / Chapter 2.8.6 --- Scintillant --- p.55 / Chapter 2.8.7 --- Trypan Blue Solution --- p.55 / Chapter 3 --- Results --- p.57 / Chapter 3.1 --- Extraction of Biologically active fractions from swiflet's nest --- p.57 / Chapter 3.2 --- Effect of SN pretreatment on Con A-stimulated Blastogenesis response in mouse splenocytes --- p.57 / Chapter 3.3 --- Chemical and Biochemical Analysis of SN extracts --- p.57 / Chapter 3.3.1 --- Atomic absorption analysis of metal elements --- p.57 / Chapter 3.3.2 --- Element analysis --- p.61 / Chapter 3.3.3 --- Biochemical analysis --- p.61 / Chapter 3.4 --- Chromatographic characterization of SN extracts --- p.67 / Chapter 3.4.1 --- Sephadex G-200 chromatography --- p.67 / Chapter 3.4.2 --- DEAE-Sepharose chromatography --- p.67 / Chapter 3.4.3 --- Immobilized Metal Affinity Chromatography --- p.73 / Chapter 3.4.4 --- Wheat-germ lectin Sepharose Chromatography --- p.73 / Chapter 3.4.5 --- Limulus Polyemus Agarose Chromatography --- p.73 / Chapter 3.4.6 --- Octyl-Sepharose Chromatography --- p.81 / Chapter 3.4.7 --- Heparin-Agarose Chromatography --- p.81 / Chapter 3.5 --- Electrophoretic Analysis of SN extract --- p.88 / Chapter 3.5.1 --- Sodium Dodecyl Sulphate/Polyacrylamide Gel Electrophoresis --- p.88 / Chapter 3.5.2 --- Isoelectrofocusing in Thin Layer of Polyacrylamide Gel --- p.88 / Chapter 3.5.3 --- Gradient Polyacrylamide Gel Electrophoresis --- p.88 / Chapter 3.6 --- Enzymatic Modification of SN extracts --- p.92 / Chapter 3.6.1 --- B-glucuronidase (EC 3.2.1.31) --- p.92 / Chapter 3.6.1.1 --- Alteration in co-mitogenic activity --- p.92 / Chapter 3.6.1.2 --- Alternation in uronic acid content --- p.92 / Chapter 3.7 --- Co-mitogenic activity of glycosaminoglycans --- p.92 / Chapter 3.8 --- Effect of heparin on the co-mitogenic activity of SNp2C fraction --- p.96 / Chapter 4 --- Discussion --- p.99 / Chapter 4.1 --- Extraction of Biologically active fraction from edible bird's nest --- p.99 / Chapter 4.2 --- Chemical and Biochemical characterization of active component(s) in SN extract --- p.100 / Chapter 4.3 --- Some mechanistic considerations --- p.107 / Chapter 4.4 --- Summary and Conclusion --- p.111 / References --- p.114
4

In vitro induction of mitogen responsiveness in murine bone marrow cells by neonatal murine thymus epithelium /

Sirinek, Lawrence Peter January 1981 (has links)
No description available.
5

A study of anti-mitogenic mechanism of epidermal growth factor /

Leung, Wing-cheung, Tommy. January 1999 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 193-205).
6

P38 MAPKs coordinately regulate distinct phases of autophagy and lysomal biogenesis

Varadarajan, Shankar 07 September 2012 (has links)
p38 mitogen-activated protein kinases (MAPKs) control the endocytic trafficking of various growth-related cell surface receptors and transporters. Herein, I demonstrate that p38 MAPKs also regulate autophagy, or the process of self-cannibalism. In my studies, inhibition of p38 MAPKs triggered rapid formation of autophagosomes in prostate cancer cells, even under nutrient-rich conditions, and remarkably, the autophagosomal membranes emanated from endoplasmic reticulum exit sites via the concerted actions of the small GTPases, ARF1 and SAR1. Once formed, the autophagosomes fused with late endosomes and/or lysosomes, in a Rab7-dependent manner, to form “hybrid organelles” that were co-labeled with ER, autophagic, late endosomal, and lysosomal markers. Unlike other inducers of autophagy, however, inhibition of p38 MAPKs suppressed the fission of hybrid organelles, resulting in a profound but reversible accumulation of large cytoplasmic vacuoles. Thus, in addition to their previously reported roles in endocytosis, p38 MAPKs appear to coordinately regulate autophagy and the downstream biogenesis and fission of hybrid organelles. / text
7

A study of anti-mitogenic mechanism of epidermal growth factor

梁永章, Leung, Wing-cheung, Tommy. January 1999 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
8

Mechanisms regulating platelet-derived growth factor-D transcription in vascular smooth muscle cells

Liu, Yanxia, Medical Sciences, Faculty of Medicine, UNSW January 2008 (has links)
Platelet-derived growth factor D-chain (PDGF-D) is the newest member of the PDGF family of mitogens and chemo-attractants; it is expressed in a wide variety of cell types, including vascular smooth muscle cells (SMCs). The molecular mechanisms regulating PDGF-D transcription are unknown. Here I investigated the effects of angiotensin II (ATIl) and IL-1 beta on the transcription of PDGF-D and changes in vascular SMCs phenotype. Primer extension analysis mapped a single transcriptional start site to the ccAG CGC motif of PDGF-D promoter. Several potential transcription factor binding sites such as SpI, Ets-1, NF-??B, IRF-1, p53, Smad4 and AP1 were located in the proximal 1168bp of the PDGF-D promoter. ATII-inducible Ets-1 and PDGF-D gene expression is mediated via H202. IL-I beta supresses PDGF-D promoter activity, mRNA and protein expression in SMCs through NF-??B p65, IRF-1 and HDAC1, which form complex in the PDGF-D promoter. This study provides the first direct link between NF-KB and the PDGF-D promoter, IRF-1 with any member of the PDGF family and a new example of HDAC mediated inhibition of gene expression. In summary, this study investigates for the first time the mechanisms mediating the transcriptional regulation of PDGF-D in vascular SMCs. This provides valuable insights into the molecular control of vascular phenotype, and opens up potential opportunities for therapeutic intervention.
9

Role of G[alpha]-interacting protein (GAIP) in modulation of MAPK pathways /

Ip, Koon-ching. January 2008 (has links)
Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2008. / Includes bibliographical references (leaves 137-156). Also available in electronic version.
10

P38 MAPKs coordinately regulate distinct phases of autophagy and lysomal biogenesis

Varadarajan, Shankar. January 1900 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

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