Impaired function of the human intestinal epithelial barrier (IEB) might allow for permeability of harmful substances, such as obesogens, which induce obesity and further implications. Tight junction (TJ) proteins are the key component for normal functions of the barrier. In this master thesis, the correlation between increased TJ permeability of the IEB and absorption of obesogens was studied. The effect of obesogens on TJ expression was also investigated. Permeability tests performed on Caco-2 cell monolayers exposed to obesogens showed altered permeability, indicating that obesogens might have an effect on TJ protein expression. Furthermore, impaired monolayers showed increased permeability, which implies that impaired functions of IEB lead to increased absorption of obesogens.

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Nela Kuchařová Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: Vasodilatory effects of bisphenol AF ex vivo Bisphenols are organic compounds used in the manufacture of plastics, resins, varnishes and lot of other products. However, their effects on human body are associated with a number of adverse effect that need to be investigated in more detail. The aim of this diploma thesis was to determine whether bisphenol AF has vasodilating effects ex vivo and to verify the mechanism of the relaxing effect. The experiments were performed on an isolated thoracic aorta of a Wistar rat. Bisphenol AF was added cumulatively to the precontracted aorta at increasing concentrations and we monitored whether vasodilation was induced. In testing the mechanism of action, we used inhibitors of the mechanisms tested during the experiment. The obtained results were evaluated using the GraphPad Prism program. The results show that bisphenol AF has a dose-dependent ex vivo vasodilatory effect (EC50 = 57.16 μmol/l). The mechanism of this effect is the blockage of voltagegated calcium channels on the vascular smooth muscle cell. The participation of other tested mechanisms has not been confirmed. Key...