The black rhinoceros remains one of the world's extremely endangered species despite a variety of policies to protect it. The black rhinoceros population at the Great Fish River Reserve (GFRR) in the Eastern Cape in South Africa has increased steadily since their re-introduction in 1986. This megaherbivore is a browser, with a diet obtained largely from the short and medium succulent thicket of the GFRR. Knowledge of the preferential diet of the black rhinoceros on the reserve is an important factor for the effective management of the land and the herbivores that compete for its resources. The dietary preferences of the black rhinoceros at the reserve have been established using backtracking methods. In this study the rbcL gene was used to establish an rbcL gene database of the plants from the GFRR and determine the botanical composition of the black rhinoceros dung from the GFRR. Due to the limited number of rbcL gene plant sequences from the GFRR deposited in the GenBank database, 18 plant species from the GFRR were sequenced. Sequence analyses between the partial rbcL gene sequences generated were able to distinguish between plants down to species level. Plant species from the family Euphorbiaceae and Fabaceae showed sequence variation at intra-specific level compared to those of Tiliaceae which were more conserved. The generated rbcL gene sequences from seasonal dung samples were compared to the rbcL gene sequenced from 18 plant species obtained from the GFRR and those from the GenBank database. A wide range of plant species were identified from the dung samples. There were no major differences in botanical composition between the dung samples, except that Grewia spp. were found to dominate in almost all seasons. The results obtained on the free radical scavenging activity of the extracts against 2,2-Diphenyl-l-picrylhydrazyl (DPPH) increased in the order of methanol > ethyl acetate > chloroform. The DPPH free radical scavenging activity of the methanol plant extracts increased in the order Brachylaena elliptica > Plumbago auriculata > Grewia robusta > Azima tetracantha. Methanol extracts on the TLC plate sprayed with Fe³⁺-2,4,6-Tri-2-pyridyl-s-triazine (TPTZ) showed that the compounds present in the extracts react differently to ferric ion, with most compounds unable to reduce ferric ion. Furthermore the methanol extracts were able to exhibit reduction potentials vs. Ag/AgCl at low concentrations. The compounds in the extracts were shown to be phenolic acids and flavonoid glycosides.

The black rhinoceros, Diceros bicornis, is listed as critically endangered. The black rhinoceros population in the Great Fish River Reserve (GFRR) has increased steadily to a current estimate of one hundred animals since the re-introduction of four animals in 1986. In an effort to contribute to the effective conservation and management of this species, dietary composition was studied in the medium Portulcaria thicket vegetation of the GFRR. This study used a molecular approach to determine the diet of the black rhinoceros of the GFRR by sequencing the ribulose bisphosphate carboxylase large subunit (rbcL) gene in plants and dung. Twenty-three plant species were collected from the reserve, and 802 bp of the rbcL gene were sequenced. These plant sequences were used as a reference database for the identification of plant sequences generated from black rhinoceros dung. Initial studies investigated the amplification, cloning and sequencing of DNA extracted from the dung samples which indicated the viability of the molecular approach. Thereafter, dung generated rbcL DNA was analyzed by GS FLX sequencing. Of the plant sequences identified by comparison to the GenBank database, Carissa bispinosa was the most prevalent. The study further characterized the antioxidant activities and phenolic content of plants eaten by the black rhinoceros using four different assays. Phyllanthus verrucosus, Putterlickia pyracantha, Maytenus capitata, Euclea undulata and Ozoroa mucrunata consistently had high antioxidant activities when assayed against 2,2-azinobis (3-ethyl benzothiazolium-6-sulfonic acid) (ABTSʹ⁺), 2,2-diphenyl-1-picrylhydrazyl (DPPHʹ), and ferric reducing antioxidant potentials (FRAP) and phenolic content when evaluated using the Folin-Ciocalteu assay. The majority of plants investigated showed low antioxidant potentials and low phenolic content. The extent to which antioxidants influenced the browse selection by the black rhinoceros remains inconclusive.

Black rhinoceroses (Diceros bicornis) are endangered; there are only ~5000 individuals remaining worldwide. Consequently, black rhinos are managed as a metapopulation in southern Africa. This means that even though several sub-populations of black rhinos are geographically separated, they are managed as a single population. This is achieved by interchanging genetic material between the sub-populations. The main aim of the metapopulation management approach is to increase black rhino numbers in the sub-region. The Rhino Management Group (RMG), formed in 1989, was tasked with setting scientifically defensible demographic targets for the remaining black rhino sub-populations in southern Africa. In the same year, the RMG set fecundity rate at 25% and stated that each black rhino sub-population should achieve a minimum growth rate of 5% per year. The black rhino population in the Hluhluwe-iMfolozi Park (HiP), KwaZulu-Natal, South Africa, is of international importance as it has historically been a significant donor population for the translocation of black rhinos to establish new subpopulations elsewhere in the sub-region. However, the black rhino population at HiP is performing poorly in terms of reproductive success. My study was initiated to assess the reproductive life-histories of the black rhino population at HiP and to determine whether the population was meeting the demographic targets set by the RMG. I also wished to assess the use of space by black rhinos in HiP as a potential explanation for the observed reproductive output. Detailed life-history information for known black rhinos (n = 120) was used to investigate reproductive success between 1998 and 2012. The mean age at sexual maturity (ASM) was determined as 12 years, which is higher than the ASM found in black rhino populations that are considered to be performing poorly (ASM = ~ 7 years). The intercalving interval (ICI) was three years, nine months; this was also longer than the ICI of most black rhino populations. Further, the 2012 black rhino population at HiP had a growth rate of 6.4%, and the population comprised 60% adults, 23% sub-adults and 13% juveniles. Such demographics are usually observed in stable populations and it is possible that poor habitat quality could be resulting in the delayed ASM and longer ICI’s at HiP. In addition, 5% of the population is removed annually as part of the Black Rhino Range Expansion Program (BRREP) to establish new sub-populations. This could also be negatively influencing the reproductive performance of the HiP population. To determine the home range and habitat use of black rhinos at HiP, field data from 13 individual adult black rhinos (n = 9 females and 4 males) over a two-year period (2011-2012) were analyzed. Black rhino female home ranges were slightly smaller (15.4 ± 12.9 km²) than the home ranges of males (23.3 ±14.0 km²) and both sexes utilized similar habitats in the centre of the park. In addition, both the male and female black rhinos were found closer to rivers and drainage lines than expected. However, the assessment of black rhino space use in HiP was limited by a small sample size, making inferences of the impact of space use on reproductive success difficult. Nevertheless, my study suggests that food availability likely influences the location and sizes of black rhino home ranges at HiP. I believe that my study forms the first step in understanding the factors that influence the reproductive success and habitat use patterns of the current black rhino population at HiP. The information produced by my study should enable park management to identify future research to be conducted at HiP, which will yield more robust data for making informed black rhino management decisions at HiP and elsewhere.

The re-introduction of black rhinoceros onto the Great Fish River Reserve in the Eastern Cape, following the species absence for over a century, created an opportunity to study this species in the thicket biome, and in particular the succulent thicket of the Great Fish River valley. The thicket biome is quite different from the habitats in which other extant populations of these animals are found and where studies of their demography have been undertaken. Data collection occurred from May 1986, with the arrival of the first four animals to December 2008, when the population was estimated to be 144 individuals. A variety of monitoring techniques were used including direct ground observations, aerial helicopter census, aerial monitoring by microlight aircraft, camera traps and opportunistic observations, each with its own advantages and disadvantages. The combination of methods and effort has resulted in the population being recognized as completely known with up to 97% of the animals being located at least once per year with a mean frequency of sightings of 11.5 per animal per year. In 2008, the population was about 20% juvenile, 36% sub-adult and 44% adult with a female biased sex ratio of 1.3 females per male. Mean age at first birth was about 80 months and this increased significantly with increasing density of black rhinoceroses. Mean intercalf interval was about 28 months and 41% of adult females gave birth each year. Mortality rates were low for juveniles, higher for sub-adults and higher for males than females. There was a weak but significant positive effect of density on male mortality. Population growth rate was about 10% and was not significantly affected by density. These results suggest that this population is beginning to show the first indications of density dependant constraints and that harvesting should be implemented in order to maintain the productive tempo. A secondary goal of the Management Plan will then be realized as the population functions as a donor to establish founder populations elsewhere within the Diceros bicornis minor range.

As part of the larger survey to map the geographical distribution of Babesia and Theileria parasites in the Southern African rhinoceros population, white rhinoceroses were sampled during routine immobilizations in the Kruger National Park. Polymerase Chain Reaction (PCR) and Reverse Line Blot (RLB) hybridization assays were used to screen for the presence of haemoprotozoa and complete blood counts were used to assess associated changes in clinical parameters. Of the 195 rhinoceroses sampled, 36.4% tested positive for the presence of Theileria bicornis, with no significant change in the haematological parameters measured. None of the rhinoceroses sampled tested positive for Babesia bicornis, the parasite linked to mortalities in black rhinoceroses. Copyright / Dissertation (MSc)--University of Pretoria, 2009. / Veterinary Tropical Diseases / unrestricted

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