Objective: To describe and compare the characteristics of blood donors and non-donors and to examine factors associated with donation, including motivators and barriers of blood donation in Guangzhou, China. Design: Cross-sectional survey using self-administered standardized structured questionnaires on both donors and non-donors. Setting: 12 mobile and 4 permanent blood donation stations in Guangzhou during the whole operation time. Participants: 500 blood donors who donated at the donation sites and 500 non-donors who never donated and passed by the station were asked to complete a self-administered questionnaire during Dec 10, 2013 to Jun 25, 2014. Main outcome measures: Blood donation or no donation. Results: 1080 questionnaires were collected, of which 1034(95.7%) questionnaires were valid. 602(58.2%) participants were donors and 432(41.8%) were non-donors. Older people (OR: 1.46, 95% confidence interval: 1.24 to 1.72, p<0.01), males (1.33, CI: 1.02 to 1.71, p=0.03), non-college-students (1.76, CI: 1.16 to 2.56, p<0.01) and people with higher education level (1.27, CI: 1.11 to 1.45, p<0.01) were more likely to be donors. The main objective of blood donation was helping patients (n=405, 68.2%), and the main reason of not donating was being in poor health (n=138, 33.1%). However, other motives, such as benefiting health and free check for blood type and body, and obstacles, such as failing to meet the requirements and fear, were also important. More male donors would donate again than females (80.5% vs. 68.5%, p<0.01), whereas more female donors showed uncertainty than males (25.9% vs. 16.6%, p<0.01). Usage of blood (n=182, 46.7%) was what non-donors wanted to know the most if they were to donate in the future. The majority of participants (n=730, 71.3%) considered raising the awareness of blood donation among people was one of the most effective ways of blood donation promotion. Television was considered as one of the most effective methods of blood donation promotion and recruitment, and was more acceptable to females. Younger participants preferred the internet. Conclusion: These findings suggest that raising the awareness of blood donation is vital. Campaigns should focus on multiple aspects targeting different groups of people. Television and the internet are useful tools of blood donation promotion and recruitment. / published_or_final_version / Public Health / Master / Master of Public Health

SUMMARY Importance of blood safety by voluntary and altruistic blood donation is presented in the Master degree thesis. The major theoretical and practical solution of blood donations adopted during the analysis of Lithuanian and foreign legislation, results of analysis of society blood donor’s motivation and blood donors focusing problems. Master degree thesis consists of four main parts: analysis of the problem, background literature review, practical research and final conclusions. The first part of the thesis emphasizes the necessity for EU member states to ensure the provision for safe blood products which quality must fulfil the same EU standards, to restore public confidence. The main problem is transition from paid and relative’s blood donations to unpaid (voluntary) donations, which is revealed in the first part of thesis. The legislation regulating blood donations, the types of current donors and factors influencing on donors motivation are discussed in the second part. The second part consists of three chapters. The review of Lithuanian legislation about donor ship in Lithuania is considered in the first chapter. It is possible to state that till the year 2004 in Lithuania legal paid blood donations system was established which today barrier in transition is from paid and relatives blood donation to unpaid (voluntary) process. The second chapter describes the changing during last 3 year among Lithuanian blood donors and in donations. on process. And the last... [to full text]

In Sweden, lowest acceptable haemoglobin levels in blood donators are 125g/L for women and 135g/L for men for a test sample taken in the beginning of the blood donation. Levels, which are 10g/L lower, are accepted if the sample is taken after the blood donation. Earlier studies show that the haemoglobin level decreases for a person that is lying down. The two aims of this study were to examine how much the haemoglobin levels change during blood donation and to examine if the photometrical instrument Pronto-7TM shows equivalent results to that of the established method Cell-Dyn Sapphire. In the study, 120 blood donors participated. Blood samples were taken in the beginning and in the end of the donation. Analyses by Pronto-7TM were done before and after the donation. The haemoglobin level decreased significantly during the blood donation. The difference was in mean value 5,9g/L according to Cell-Dyn Sapphire. The decrease was significantly less than 10g/L. The Pronto-7TM gave levels that were 1,6g/L higher than Cell-Dyn Sapphire in mean and the standard deviation was higher for Pronto-7TM than for Cell-Dyn Sapphire. In conclusion, the decrease of the haemoglobin levels was significantly less than the expected difference 10g/L. Pronto-7TM gives results that differs a little from the results of the established method.

This thesis explored the impact on whole blood donors of a six month deferral from giving blood due to a low haemoglobin (Hb) concentration. The aims were two-fold: first, to quantify the effect of a temporary deferral on donation patterns once eligible to return, and second, to identify the processes contributing to the effect. The mixed methods design utilised four distinct research phases: statistical analysis of donation patterns over a three year period, surveys of whole blood donors three and twelve months after deferral, and semi-structured interviews with 25 blood donors in the weeks immediately following deferral. Deferral for a low Hb increased the likelihood of non-return in both new and repeat donors, and, amongst those who did return, delayed first return, reduced donation frequency and increased the likelihood of drop-out in later years. Qualitative interviews suggested that, predominantly, individuals give blood because it represents an easy and convenient way to help others, and provides additional rewards, such as enhancing positive self-concepts and a free health check. Returning promptly after deferral appears to be related to three aspects of a person and his/her context: an individual’s other obligations, especially parenting; the extent to which donation is considered personally rewarding; and whether donation arrangements were facilitated by a range of supports prior to deferral. Over three quarters of surveyed deferred donors seek further advice and investigations from their medical practitioner and nearly half of those are encouraged to change their donation patterns. With the exception of having a low haemoglobin level confirmed at follow-up testing, experiences seeking further investigations were not associated with either intentions or return. Triangulation of findings suggests that deferral disrupts the habit of regular donation, and that this disruption makes donors more vulnerable to changes to their personal circumstances or collection practices. Deferral may also increase the perceived inconvenience of the activity, decrease self-perceptions of competence and good health, and diminish the “blood donor” identity. Practical implications of these findings are recommendations that may increase retention of deferred donors, including encouraging donors to return promptly once eligible, enhancing the convenience of blood donation, and improving aspects of the deferral event. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1381077 / Thesis (Ph.D.) - University of Adelaide, School of Population Health and Clinical Practice, 2010

This thesis explored the impact on whole blood donors of a six month deferral from giving blood due to a low haemoglobin (Hb) concentration. The aims were two-fold: first, to quantify the effect of a temporary deferral on donation patterns once eligible to return, and second, to identify the processes contributing to the effect. The mixed methods design utilised four distinct research phases: statistical analysis of donation patterns over a three year period, surveys of whole blood donors three and twelve months after deferral, and semi-structured interviews with 25 blood donors in the weeks immediately following deferral. Deferral for a low Hb increased the likelihood of non-return in both new and repeat donors, and, amongst those who did return, delayed first return, reduced donation frequency and increased the likelihood of drop-out in later years. Qualitative interviews suggested that, predominantly, individuals give blood because it represents an easy and convenient way to help others, and provides additional rewards, such as enhancing positive self-concepts and a free health check. Returning promptly after deferral appears to be related to three aspects of a person and his/her context: an individual’s other obligations, especially parenting; the extent to which donation is considered personally rewarding; and whether donation arrangements were facilitated by a range of supports prior to deferral. Over three quarters of surveyed deferred donors seek further advice and investigations from their medical practitioner and nearly half of those are encouraged to change their donation patterns. With the exception of having a low haemoglobin level confirmed at follow-up testing, experiences seeking further investigations were not associated with either intentions or return. Triangulation of findings suggests that deferral disrupts the habit of regular donation, and that this disruption makes donors more vulnerable to changes to their personal circumstances or collection practices. Deferral may also increase the perceived inconvenience of the activity, decrease self-perceptions of competence and good health, and diminish the “blood donor” identity. Practical implications of these findings are recommendations that may increase retention of deferred donors, including encouraging donors to return promptly once eligible, enhancing the convenience of blood donation, and improving aspects of the deferral event. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1381077 / Thesis (Ph.D.) - University of Adelaide, School of Population Health and Clinical Practice, 2010

Foram analisadas 197.032 doações no período de janeiro de 1998 a junho de 2008, realizadas no Banco de Sangue do Hospital de Clínicas de Porto Alegre. Os doadores de sangue foram triados por um teste de enzimaimunoensaio (EIE) de terceira geração para HTLV I/II. Também foram realizados testes para hepatites B e C, HIV 1/2, sífilis e doença de Chagas. Os dados das fichas cadastrais foram revisados após o diagnóstico do doador. Como teste confirmatório, foi utilizado o Western Blot (WB). Foram excluídos os doadores considerados negativos após a confirmação dos testes iniciais. Ao todo, 204 doadores foram reagentes para HTLV I ou II. A prevalência de HTLV I/II foi de 0,1%, sendo 0,08% do tipo I e 0,02% do tipo II. A média de idade foi de 38 anos; 73,5% dos doadores eram brancos e 53,9% do sexo feminino. Cerca de 65% deles completaram o ensino fundamental e apenas 1% o ensino superior; 59,3% eram procedentes de Porto Alegre. A frequência de casados e solteiros foi de 40,2% e 42,4%, respectivamente, sendo os demais separados, divorciados ou viúvos. Os soropositivos para HTLV apresentaram ainda 30,4% de coinfecção com os outros marcadores testados. AntiHCV e anti-HBc foram os marcadores com maior prevalência (19,1% e 18,6%, respectivamente), seguidos por VDRL (2,94%), anti-HIV 1/2 (2,45%) e doença de Chagas (1,96%). / A total of 197,032 donors from the Blood Bank of Hospital de Clínicas de Porto Alegre, in southern Brazil, were assessed from January 1998 to June 2008. Blood donors were screened for HTLV I/II using a third-generation enzyme immunoassay (EIA). Tests for hepatites B and C, HIV 1 and 2, syphilis and Chagas disease were also performed. The information on blood donor forms was reviewed after the diagnosis. The Western Blot (WB) was used as confirmatory test. Seronegative donors were excluded from the study after initial test results were confirmed. Two hundred and four donors were positive for HTLV I or II. The prevalence of HTLV I/II was 0.1% (0.08% for HTLV I and 0.02% for HTLV II). The mean age was 38 years, 73.5% were whites and there was a female predominance (53.9%). Approximately 65% of seropositive individuals had attended elementary school, only 1% had college education, and 59.3% came from Porto Alegre. The frequency of married and single individuals was similar (40.2 and 42.4%, respectively), whereas the remaining donors were separated, divorced or widowed. This population also showed 30.4% of coinfection, according to the other tested markers. Anti-HCV and anti-HBc were the most prevalent markers (19.1 and 18.6%, respectively), followed by VDRL (2.94%), anti-HIV 1/2 (2.45%), Chagas disease (1.96%).

Foram analisadas 197.032 doações no período de janeiro de 1998 a junho de 2008, realizadas no Banco de Sangue do Hospital de Clínicas de Porto Alegre. Os doadores de sangue foram triados por um teste de enzimaimunoensaio (EIE) de terceira geração para HTLV I/II. Também foram realizados testes para hepatites B e C, HIV 1/2, sífilis e doença de Chagas. Os dados das fichas cadastrais foram revisados após o diagnóstico do doador. Como teste confirmatório, foi utilizado o Western Blot (WB). Foram excluídos os doadores considerados negativos após a confirmação dos testes iniciais. Ao todo, 204 doadores foram reagentes para HTLV I ou II. A prevalência de HTLV I/II foi de 0,1%, sendo 0,08% do tipo I e 0,02% do tipo II. A média de idade foi de 38 anos; 73,5% dos doadores eram brancos e 53,9% do sexo feminino. Cerca de 65% deles completaram o ensino fundamental e apenas 1% o ensino superior; 59,3% eram procedentes de Porto Alegre. A frequência de casados e solteiros foi de 40,2% e 42,4%, respectivamente, sendo os demais separados, divorciados ou viúvos. Os soropositivos para HTLV apresentaram ainda 30,4% de coinfecção com os outros marcadores testados. AntiHCV e anti-HBc foram os marcadores com maior prevalência (19,1% e 18,6%, respectivamente), seguidos por VDRL (2,94%), anti-HIV 1/2 (2,45%) e doença de Chagas (1,96%). / A total of 197,032 donors from the Blood Bank of Hospital de Clínicas de Porto Alegre, in southern Brazil, were assessed from January 1998 to June 2008. Blood donors were screened for HTLV I/II using a third-generation enzyme immunoassay (EIA). Tests for hepatites B and C, HIV 1 and 2, syphilis and Chagas disease were also performed. The information on blood donor forms was reviewed after the diagnosis. The Western Blot (WB) was used as confirmatory test. Seronegative donors were excluded from the study after initial test results were confirmed. Two hundred and four donors were positive for HTLV I or II. The prevalence of HTLV I/II was 0.1% (0.08% for HTLV I and 0.02% for HTLV II). The mean age was 38 years, 73.5% were whites and there was a female predominance (53.9%). Approximately 65% of seropositive individuals had attended elementary school, only 1% had college education, and 59.3% came from Porto Alegre. The frequency of married and single individuals was similar (40.2 and 42.4%, respectively), whereas the remaining donors were separated, divorced or widowed. This population also showed 30.4% of coinfection, according to the other tested markers. Anti-HCV and anti-HBc were the most prevalent markers (19.1 and 18.6%, respectively), followed by VDRL (2.94%), anti-HIV 1/2 (2.45%), Chagas disease (1.96%).

Made available in DSpace on 2014-06-11T19:23:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-08-27Bitstream added on 2014-06-13T18:09:20Z : No. of bitstreams: 1 bueno_l_me_botfm.pdf: 328709 bytes, checksum: 085dc669d232db13d9fdf6315c4a5245 (MD5) / Fundação Amaral Carvalho / O Ministério da Saúde muito tem se preocupado com a segurança transfusional, tendo a utilização da ferramenta “Voto de Autoexclusão – VAE” como mecanismo de barreira na transmissão de doenças, visto que A triagem sorológica em doadores de sangue não possibilita segurança de 100% quanto a possibilidade de transmissão de agentes infecto-contagiosos. O objetivo desse trabalho foi avaliar o índice de autoexclusão (AE) no Hemonúcleo Regional de Jaú no período de 2006 a 2010, de acordo com o tipo de doador, gênero, faixa-etária, estado civil, tempo de doação na unidade, procedência e comparar a frequência dos testes sorológicos reagente entre doadores que se AE e não se auto-excluíram. De 2006 a 2010, o Hemonúcleo Regional de Jaú coletou 42.509 bolsas de sangue, das quais 818(2%) doações foram desprezadas pelo VAE. Estas 818 doações correspondem a 742 doadores devido ao fato de que 26 doadores se AE mais de uma vez no período da pesquisa. A taxa de AE no grupo masculino foi significativamente maior que no grupo feminino, bem como a chance de AE neste grupo foi 1,9 vezes maior que no grupo feminino. Quanto à faixa etária, a taxa de AE foi maior em doadores mais jovens (18 a 29 anos). Quanto à raça, houve um equilíbrio entre brancos, negros e pardos. Em relação ao estado civil, doadores casados são menos frequentes que solteiros e indivíduos com outros tipos de relacionamento. A chance de se autoexcluírem em doadores de 1ª vez foi de 1,7 vezes maior do que no grupo de repetição. Este resultado pode evidenciar uma falta de entendimento do doador quanto ao significado do VAE, além de mostrar a importância de se elaborar um instrumento auto-explicativo que facilite a compreensão do doador. Quanto ao índice de soropositividade para o conjunto de marcadores de doenças... / The ministry of health has long been concerned about the safety of blood transfusion, and the use of the tool vow of self-exclusion - VAE as a barrier machanism in the transmission of diseases, considering that serologial screening of blood donors does not allow 100% security as possibility of transmission of infectious and contagious agents. The aim of this study was to evaluate the index of self-exclusion (AE) in Hemonúcleo Regional of Jaú, in the period of 2006 to 2010, according to the donor type, gender, age group, marital status, time of donation in the unit, procedure and comparison of serologic frequency, testing reagents among donors that self-excluded themselves and those who didn't. From 2006 to 2010, the Hemonúcleo Reginal of Jaú collected 42.509 units of blood, of which 818 (2%) donations were discarted by a vote of self-exclusion. These 818 donations from 742 donors match due to the fact that 26 donors excluded themselves more than once in the period of this research.The rate of self-exclusion in the male group was significantly higher than in the female group, as well as the chance of self-exclusion in this group was 1.9 times higher than in the female group.The age group of 60 to 69 years predominates over the age group of 18 to 49 years. As for the race group, there was a balance between white, black and brown. Regarding marital status, married donors are less frequent than single and relationships with other individuals. The chance of self-excluded donors at a first time was 1.7 times higher than in the repeating group. This result may show a lack of understanding of the donors as to the meaning of the vow of self exclusion, and show the importance of preparing a self explanatory that facilitates understanding of the donor. In the rate of seropositivity for all markers of viral diseases was found that the chance of positive... (Complete abstract click electronic access below)

Orientador: Rosana Rossi Ferreira / Banca: Marjorie de Assis Golim / Banca: Michele Janegitz Acorse Valério / Resumo: O Ministério da Saúde muito tem se preocupado com a segurança transfusional, tendo a utilização da ferramenta "Voto de Autoexclusão - VAE" como mecanismo de barreira na transmissão de doenças, visto que A triagem sorológica em doadores de sangue não possibilita segurança de 100% quanto a possibilidade de transmissão de agentes infecto-contagiosos. O objetivo desse trabalho foi avaliar o índice de autoexclusão (AE) no Hemonúcleo Regional de Jaú no período de 2006 a 2010, de acordo com o tipo de doador, gênero, faixa-etária, estado civil, tempo de doação na unidade, procedência e comparar a frequência dos testes sorológicos reagente entre doadores que se AE e não se auto-excluíram. De 2006 a 2010, o Hemonúcleo Regional de Jaú coletou 42.509 bolsas de sangue, das quais 818(2%) doações foram desprezadas pelo VAE. Estas 818 doações correspondem a 742 doadores devido ao fato de que 26 doadores se AE mais de uma vez no período da pesquisa. A taxa de AE no grupo masculino foi significativamente maior que no grupo feminino, bem como a chance de AE neste grupo foi 1,9 vezes maior que no grupo feminino. Quanto à faixa etária, a taxa de AE foi maior em doadores mais jovens (18 a 29 anos). Quanto à raça, houve um equilíbrio entre brancos, negros e pardos. Em relação ao estado civil, doadores casados são menos frequentes que solteiros e indivíduos com outros tipos de relacionamento. A chance de se autoexcluírem em doadores de 1ª vez foi de 1,7 vezes maior do que no grupo de repetição. Este resultado pode evidenciar uma falta de entendimento do doador quanto ao significado do VAE, além de mostrar a importância de se elaborar um instrumento auto-explicativo que facilite a compreensão do doador. Quanto ao índice de soropositividade para o conjunto de marcadores de doenças... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The ministry of health has long been concerned about the safety of blood transfusion, and the use of the tool "vow of self-exclusion - VAE" as a barrier machanism in the transmission of diseases, considering that serologial screening of blood donors does not allow 100% security as possibility of transmission of infectious and contagious agents. The aim of this study was to evaluate the index of self-exclusion (AE) in Hemonúcleo Regional of Jaú, in the period of 2006 to 2010, according to the donor type, gender, age group, marital status, time of donation in the unit, procedure and comparison of serologic frequency, testing reagents among donors that self-excluded themselves and those who didn't. From 2006 to 2010, the Hemonúcleo Reginal of Jaú collected 42.509 units of blood, of which 818 (2%) donations were discarted by a vote of self-exclusion. These 818 donations from 742 donors match due to the fact that 26 donors excluded themselves more than once in the period of this research.The rate of self-exclusion in the male group was significantly higher than in the female group, as well as the chance of self-exclusion in this group was 1.9 times higher than in the female group.The age group of 60 to 69 years predominates over the age group of 18 to 49 years. As for the race group, there was a balance between white, black and brown. Regarding marital status, married donors are less frequent than single and relationships with other individuals. The chance of self-excluded donors at a first time was 1.7 times higher than in the repeating group. This result may show a lack of understanding of the donors as to the meaning of the vow of self exclusion, and show the importance of preparing a self explanatory that facilitates understanding of the donor. In the rate of seropositivity for all markers of viral diseases was found that the chance of positive... (Complete abstract click electronic access below) / Mestre

Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2018-09-04T17:25:56Z No. of bitstreams: 2 Tese - Ivonizete Pires Ribeiro - 2014.pdf: 2274390 bytes, checksum: cf28ad8a72a27c4a3721c8fa2ba1c72b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-09-05T11:19:01Z (GMT) No. of bitstreams: 2 Tese - Ivonizete Pires Ribeiro - 2014.pdf: 2274390 bytes, checksum: cf28ad8a72a27c4a3721c8fa2ba1c72b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-09-05T11:19:01Z (GMT). No. of bitstreams: 2 Tese - Ivonizete Pires Ribeiro - 2014.pdf: 2274390 bytes, checksum: cf28ad8a72a27c4a3721c8fa2ba1c72b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2014-12-15 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Human T-lymphotropic virus type (HTLV-1) is the etiological agent of adult T-cell leukemia/lymphoma (ATL), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), and other inflammatory diseases. HTLV-2 has been associated with a syndrome similar to HAM/TSP and other clinical manifestations. The present study aimed to investigate the seroepidemiologic and molecular aspects of HTLV-1/2 infection in the first-time blood donors and their family members in the state of Piauí. Between August 2011 and July 2012, 37,306 first-time blood donors were screened by enzyme-linked immunosorbent assay (ELISA) for the presence of anti-HTLV-1/2 antibodies in the Centro de Hematologia e Hemoterapia do Estado do Piauí (HEMOPI). The positive individuals were recruited for a second blood collection and interview, when their family members were invited to participate to this investigation. Sera were tested by ELISA, and the positivity confirmed by line immunoassay (LIA). Whole-blood samples of anti-HTLV seropositive individuals were submitted to proviral DNA detection by polymerase chain reaction (PCR) and nucleotide sequencing, followed by phylogenetic analysis. Of 37,306 first-time blood donors, 48 were anti-HTLV-1/2 reactive by ELISA. By testing the second serum samples, 47 (0.13%) were also reactive by ELISA. Among these, 22 (0.06%) were anti-HTLV-1 and 14 (0.04%) anti-HTLV-2 positive by LIA. Most of HTLV-1 or 2 infected patients reported risk characteristics related to vertical and sexual transmission. Genetic characterization demonstrated that all 22 HTLV-1 isolates belonged to the Cosmopolitan subtype HTLV-1a Transcontinental subgroup A. For HTLV-2, 11/14 (78.6%) anti-HTLV-2 positive samples were amplified and then identified as subtype a (HTLV-2 a/c). Of 21 families invited to take part in the study, five agreed to participate. Three had, in addition to the index case (blood donor), two or more anti-HTLV-1 or 2-positive members (ELISA/LIA). By combining epidemiological data of the first family members with molecular analysis of their isolates, intrafamilial/vertical transmission of HTLV-1 was suggested, as well as the transmission of HTLV-2 among the second and the third families. Additionally, HTLV-2 sexual transmission could be considered in the last family. The prevalence of 0.13% (CI 95%: 0.11-0.17) for HTLV-1/2 found in this study is similar to those observed in blood donors in Brazil, and HTLV-1 and 2 subtypes identified in this investigation were consistent with those circulating in Brazil. In addition, this study provides evidence of intrafamilial transmission of HTLV-1 and 2. / O vírus linfotrópico de células T humanas 1 (HTLV-1) é o agente etiológico da leucemia de células T do adulto (ATL) e mielopatia associada ao HTLV-1/paraparesia espástica tropical (HAM/TSP), além de outras doenças inflamatórias. O HTLV-2 tem sido associado com síndrome similar a HAM/TSP e outras manifestações clínicas. O presente estudo tem como objetivo investigar os aspectos soroepidemiológicos e moleculares da infecção pelo HTLV-1/2 em primodoadores de sangue e seus familiares no Estado do Piauí. No período de agosto/2011 a julho/2012, 37.306 primodoadores de sangue foram triados para detecção de anticorpos anti-HTLV-1/2 por ensaio imunoenzimático (ELISA), no Centro de Hematologia e Hemoterapia do Estado do Piauí (HEMOPI). Os indivíduos reagentes foram recrutados para segunda coleta de sangue e entrevista, ocasião que seus familiares foram convidados para participar da investigação. Os soros foram testados por ELISA, e a reatividade confirmada pelo line immunoassay (LIA). As amostras de sangue total dos indivíduos anti-HTLV-1/2 reagentes foram submetidas à detecção do DNA proviral por reação em cadeia pela polimerase (PCR) e sequenciamento, seguido da análise filogenética. Dos 37.306 primodoadores de sangue triados para anti-HTLV-1/2, 48 foram reagentes pelo ELISA. Ao testar a segunda amostra desses indivíduos também por ELISA, 47 (0,13%) permaneceram como reagentes. Destas, 22 (0,06%) foram anti-HTLV-1 e 14 (0,04%) anti-HTLV-2 positivas pelo LIA. A maioria dos doadores infectados pelo HTLV-1 ou 2 relatou características de risco relacionadas à transmissão vertical e sexual. A caracterização genética demonstrou que os 22 isolados do HTLV-1 foram pertencentes ao subtipo Cosmopolita (HTLV-1a) do subgrupo Transcontinental (A). Para o HTLV-2, 11/14 (78,6%) amostras anti-HTLV-2 positivas foram amplificadas e, posteriormente, caracterizadas como do subtipo a (HTLV-2a/c). De 21 famílias dos primodoadores de sangue HTLV-1/2 soropositivos contactadas, cinco concordaram em participar do estudo. Três famílias apresentaram, além do caso índice (doador), pelo menos dois outros membros anti-HTLV-1 ou 2 soropositivos (ELISA/LIA). Combinando os dados epidemiológicos dos membros da primeira família com a análise molecular de seus isolados, foi evidenciada a ocorrência de transmissão intrafamiliar/vertical do HTLV-1 e, na segunda e terceira famílias, do HTLV-2. Adicionalmente, nesta última, a transmissão sexual do HTLV-2 pode ser considerada. A prevalência encontrada para o HTLV- 1/2 no presente estudo (0,13%; IC 95%: 0,09-0,17) é concordante com as estimadas em doadores de sangue no Brasil, e os subtipos do HTLV-1 e 2 identificados nesta investigação corroboram a predominância desses no País. Este estudo fornece, ainda, evidência de transmissão intrafamiliar do HTLV-1 e 2.