Thesis (D.P.E.)--Springfield College, 2005. / Includes bibliographical references.

Thesis (M.S.Ed.)--Baylor University, 2006. / Includes bibliographical references (p. 81-84).

The aim of this thesis was to describe the relationships between energy expenditure and body composition in healthy adults and children and in patients with metabolic disorders. In a healthy population resting energy expenditure (REE) is highly influenced by body composition, specifically lean mass (LM). Prediction equations can therefore accurately predict REE from body composition in healthy individuals. However, application of these equations to clinical patients, in whom metabolism is disordered, risks miscalculation of energy metabolism due to their dissociation between body composition and energy expenditure. Therefore new prediction equations were derived based on precise body composition measurements in healthy adults and children. Then, in patients with metabolic disorders, differences between their measured and equation-predicted REE and LM were presented as standardised Z scores. REE in healthy adults was predicted by the coefficients: age, fat mass and fat-free mass. LM in healthy adults was predicted by the coefficients: bone mineral content and height2 in men; and by fat and height2 in women. In healthy children, REE was predicted using gender specific models: by fat and LM in boys; and by solely LM in girls. REE and LM were then measured in adult and paediatric patients with metabolic disorders (Lipodystrophy, Thyrotoxicosis and Resistance to Thyroid Hormone β or α), and Z scores were calculated to highlight their deviations from the healthy populations. In adults, thyrotoxicosis patients displayed the highest REE Z scores (5.8), followed by lipodystrophy (2.9) and RTHβ cases (1.8), with RTHα demonstrating the lowest REE Z scores (-2.3). For LM, lipodystrophy patients exhibited with the highest Z scores (4.2), followed by RTHα patients (2.1), with RTHβ patients showing normal LM Z scores (-0.2) and thyrotoxicosis patients presenting with the lowest LM Z scores (-1.2). In the paediatric patients, RTHβ patients demonstrated REE Z scores similar to healthy controls (males; -0.15, females; 0.15), but RTHα patients displayed lower REE Z scores (male; -0.82, female; -2.2) compared to RTHβ patients and healthy controls. These studies highlight the disassociation between REE and body composition in patients with metabolic disorders. The application of a prediction equation for REE to calculate Z scores between measured and predicted values allows quantification of the differences between patients with metabolic disorders and healthy populations, and is a new and important concept.

Made available in DSpace on 2014-06-11T19:28:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-16Bitstream added on 2014-06-13T20:18:01Z : No. of bitstreams: 1 boaventuraneto_o_me_jabo.pdf: 214994 bytes, checksum: 5d85c477b6d5ce4ed1965af46a9008a1 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O objetivo deste estudo foi avaliar o efeito do sexo na composição corporal e exigência de proteína para mantença e ganho, em caprinos Saanen na fase inicial de crescimento. Foram realizados dois experimentos, em que no primeiro utilizou-se 40 animais (14 machos não castrados, 14 machos castrados e 12 fêmeas) com peso inicial de 4,93 ± 0,1 kg de peso corporal (PC), para determinar a exigência de proteína para mantença. Os animais de cada sexo foram aleatoriamente distribuídos em 3 níveis de restrição (0, 25 e 50% de restrição). Os animais 25 e 50% de restrição foram abatidos quando os animais 0% de restrição atingiram 15 kg PC. No segundo experimento, considerou-se para a estimativa da composição do ganho os animais alimentados à vontade (abate inicial, intermediário e final - 0% restrição), em que 6 machos não castrados e 6 fêmeas foram abatidos no começo do experimento (5 kg), 6 machos não castrados, 6 machos castrados e 6 fêmeas foram abatidos com 10 kg PC (abate intermediário) e o restante foi distribuído em 3 grupos alimentados à vontade e abatido aos 15 kg PC. Foi utilizado o método direto para determinação da composição corporal e o método fatorial para estimativa das exigências de proteína. A quantidade de água, proteína e cinzas não diferiu entre os sexos e diminuiu em 1%, 62% e 17% com a mudança do PC de 5 para 15 kg respectivamente. Já em relação ao conteúdo de gordura, conforme o PC variou de 5 para 15 kg, os machos não castrados e machos castrados aumentaram de 54,6 para 88,17 g/kg PCV e as fêmeas aumentaram de 45,1 para 117,67 g/kg PCV. Não houve efeito do sexo sobre a exigência de proteína para mantença e nem para ganho de peso. A exigência de proteína líquida e metabolizável para ganho diminuiu à medida que o PC variou de 5 para 15 kg. Cabritos Saanen... / The aim of this study was to evaluate the effect of sex on body composition and nutritional requirement of protein for maintenance and gain in Saanen goats at early growth stages. There were two different experiments, the first was used 40 animals (14 non-castrated males, 14 castrated males and 12 females) with initial weight of 4.93 ± 0.1 kg body weight (BW), to determine the protein requirement for maintenance. The animals of each sex were randomly divided into three levels of restriction (0%, 25% and 50% restriction). Animals with 25% and 50% restriction were slaughtered when the animals who were feed with 0% restriction reached 15 kg BW. In the second experiment to estimate the composition of the gain was used animals feed ad libitum (initial slaughter, intermediate and final - 0% restriction), in which 6 non-castrated males and 6 females were slaughtered at the beginning of the experiment, 6 non-castrated males, 6 castrated males and 6 females were slaughtered at 10 kg BW (intermediary slaughter) and the remainder distributed in three groups feed ad libitum. Was used the comparative slaughter technique for obtaining the body composition and the factorial method for estimating protein requirements. The amount of water, protein and ash decreased by 1%, 62% and 17% with the change of PC from 5 to 15 kg and did not differ between the sexes. In relation to fat, as the PC ranged from 5 to 15 kg, non-castrated males and castrated males increased from 54.6 to 88.17 g / kg EBW and females increased from 45.1 to 117.67 g / kg EBW. No effect of sex in the protein requirement for maintenance and for gain. The net requirement for protein and metabolizable gain decreased as the PC ranged from 5 to 15 kg. Saanen goats non-castrated males, castrated males and females at early growth stages (5 to 15 kg BW), have the same nutritional requirement of protein for maintenance and gain

Calcium (Ca) intakes may affect cardiovascular disease risk by altering body weight/fat and serum lipid profile, but results have been inconsistent and the underlying mechanisms are not well understood. Thus, the effects of dietary Ca on body composition and lipid metabolism were examined in male Sprague-Dawley rats. Rats were fed high-fat, high-energy diets containing (g/kg) low (0.75Ca, 0.86 ± 0.05; 2Ca, 2.26 ± 0.02), normal (5Ca, 5.55 ± 0.08) or high (10Ca,11.03 ± 0.17; 20Ca, 21.79 ± 0.15) Ca for 10 weeks. At the end of the study the 0.75Ca group had lower (p < 0.05) body weight and fat mass compared to other groups. Rats fed the high Ca diets had lower serum total and LDL cholesterol compared to rats fed normal or low Ca. Liver total cholesterol was lower in rats fed high compared to low Ca. In general, liver mRNA expression of the LDLR and genes involved in cholesterol synthesis (HMGCR and HMGCS1), fatty acid oxidation (CPT2) and cholesterol esterification (ACAT2) were higher in rats fed higher Ca. Apparent digestibility of total trans, saturated, monounsaturated and polyunsaturated fatty acids was lower in rats fed the high compared to the low Ca diets, but the differences were greatest for trans and saturated fatty acids. Fecal excretion of cholesterol and total bile acids was highest in rats fed the 20Ca diet. The results suggest little effect of dietary Ca on body composition unless Ca intakes are very low. Decreased bile acid reabsorption and reduced absorption of neutral sterols and trans and saturated fatty acids may contribute to the improved serum lipid profile in rats fed higher Ca.

M.Tech. / Obesity is a serious health problem throughout the world, with the number of cases having increased three-fold over the last two decades, reaching epidemic levels in the United States (Kumar and Clark, 2009). According to the South African Demographic and Health Survey (SADHS) of 2002, 29.2% of South African men were overweight, whereas 56.6% of women were overweight. Obesity leads to a decreased quality of life and can have a detrimental effect on general well being (SADHS, 2002). Overweight and obesity have major psychological effects on the individual. Obese individuals often suffer from body image issues, unhappiness, a decreased morale, low self esteem and eating disorders (Rogge et al., 2004). The stigma associated with adolescent obesity causes bullying in school as well as childhood psychiatric morbidity (Gortmaker et al., 1993). The aim of this study was to determine the effect of an herbal formulation consisting of Coleus Forskohlii, Camellia sinensis, Coffea canephora, Caffeine, Evodiamine, Ilex paraguariensis and Phaseolus vulgaris on general well being in overweight and obese individuals. This study was a quantitative double-blind, placebo-controlled study, using matched pairs according to gender and body mass index (BMI). The study was conducted over twelve weeks during the period of October 2011 to January 2012 at the University of Johannesburg’s Homeopathy Clinic. The conducted study formed part of a larger study where the research sample was shared by two additional researchers (Durrheim et al., 2012) (Withers et al., 2012). This allowed for a larger sample group to be tested with numerous variables being researched. The study included sixty overweight and obese males and females between the ages of eighteen and forty five, with a BMI above 25 kg/m² and under 35kg/m². Participants were required to sign a Participant Information and Consent form (Appendix B) giving the necessary information regarding this study. The groups were randomly matched assigned by the dispenser (according to the participant’s BMI) to the experimental group or placebo group, each consisting of thirty participants. The medication was randomised by the homeopathic dispenser at the University of Johannesburg’s Homeopathy Clinic. The experimental group received a bottle of capsules containing the herbal formulation, and the placebo received identical looking placebo capsules containing a pharmaceutical maize starch.

Gentamicin is an aminoglycoside antibiotic that possesses bactericidal activity against many gram-positive and gram-negative organisms. Clinically, it is used most often to treat life-threatening infections due to Pseudomonas, Proteus, and the Klebsiella-Enterobacter group. A relationship between gentamicin serum concentrations and clinical response has been demonstrated. Toxicities, notably ototoxicity and nephrotoxicity, are also associated with serum concentrations. Gentamicin is given intermittently either intramuscularly or intravenously resulting in peak and trough concentrations. The therapeutic range is defined as peak concentrations between 4-15mg/L (depending in part on the site of infection and the susceptibility of the infecting organism), and trough concentrations less than 2mg/L (to minimize toxicity). Gentamicin distributes into a space similar to the extracellular fluid volume (ECFV). Pathophysiologic changes which alter the extracellular fluid compartment also alter gentamicin volume of distribution (Vd). One intrinsic factor known to alter gentamicin Vd is obesity. Leanness is also thought to alter gentamicin Vd but its effect has not been quantitated. The objectives of this study were to: 1) accurately describe a Vd in "normal" patients, that is, those with no factors known to alter gentamicin volume of distribution; 2) determine if there is a continuous linear relationship between gentamicin volume of distribution (L/kg) and percent body fat; 3) determine if that relationship is associated with changes in ECFV; and 4) develop a formula for predicting Vd in a similar patient population. Twenty patients with no extrinsic factors known to alter gentamicin Vd participated in the study. Five blood samples were drawn around one steady state dose of gentamicin. A one-compartment model was used to calculate Vd. Tritiated water and anthropometric measurements were conducted simultaneously to provide estimates of body composition. Together these values were used to examine the relationship between gentamicin Vd and body composition. We have described a Vd for gentamicin that is larger but no less variable than is currently used to determine initial dosage regimens. This volume may be larger either due to the selection of patients or method of serum gentamicin analysis. This larger volume should be used to calculate empiric dosage regimens for similarly selected patients to decrease the risk of treatment failure. We were not able to describe a linear relationship between percent body fat and gentamicin volume of distribution. We have postulated several reasons as to why this relationship could not be detected; 1) the sample size may not have been large enough, 2) the relationship is not important in patients who are not at extremes of weight, or 3) the variations caused by changes in body composition were not as significant as other factors that may cause fluid alterations in hospitalized patients. There was a strong correlation between gentamicin Vd and total body water noted. Having eliminated all patients in whom the relationship between total body water and ECFV could not be assumed to be normal and constant, we have indirectly demonstrated a strong relationship between ECFV and gentamicin Vd. This relationship still leaves variability in gentamicin's distribution characteristics to be explained. The predictive formula is based on measurements of height, weight, and a larger Vd [L/kg(ideal body weight)] than has previously been used. The predictive formula recommended for clinical use in adults is Vd=0.30L/kg (Dosing Weight). Dosing weight equals ideal body weight (IBW) when actual body weight (ABW) is ≤ IBW, or 0.4(ABW-IBW)+IBW, when ABW is > IBW. The consequences of estimating a larger Vd are that patients empirically would receive larger doses than are currently being administered, thus more patients should obtain therapeutic serum concentrations within the first 24 hours of therapy. This information will be useful in our attempts to optimize gentamicin therapy. / Pharmaceutical Sciences, Faculty of / Graduate

Since no lean body mass (LBM) index was available for women, the purpose of the present study was to use the formula of Behnke et al. in the calculation of an LBM Index for women.

The aim of the work was to evaluate significant changes in body composition in Czech breastfeeding women during lactation and to confront them with the already available results of other similar studies. For this purpose, 10 female participants of the longitudinal study were selected. Body composition was determined by bioimpedance spectroscopy with a Fresenius Body Composition Monitor. Furthermore, anthropometric methods were used to evaluate the basic body parameters and compare their dependent loss with the values measured by BCM. The measurement was repeated in four periods - 3 weeks, 3 months, 6, and 9 months after delivery. During the observed period, an overall decrease in median weight of 4,2 kg was observed in lactating women. The median weight 9 months postpartum was still 1,2 kg greater than the median weight before pregnancy. No statistically significant differences in body composition were found during lactation. Human milk volume correlated positively with age of women (p = 0,0404; r = 0,3386), negatively with calf circumference (p = 0,0313; r = -0,3545), chin skinfold (p = 0,0198; r = -0,3816), triceps skinfold (p = 0,0905; r = -0,3302), LTI (p = 0,0216; r = -0,3767) and Cm (p = 0,0490; r = -0,3260). Correlations were also found between milk volume and some values needed to calculate...

Master of Science