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abstract / Biochemistry / Master / Master of Philosophy

published_or_final_version / Biochemistry / Master / Master of Philosophy

Bibliography: leaves 298-334. / xxxi, 334, [3] leaves, [18] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Provides insight into the mechanism by which dihydrotestosterone (DHT) and estrogen effect osteoblast and osteoclast bone cell activities. Also demonstrates the differential effects of DHT on bone cell activities in estrogen sufficient and estrogen deficient rats suggesting that there is an interaction of estrogens and androgens on bone cell metabolism in the female rat. / Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 1998?

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / The mammalian hyaluronidases represent a family of enzymes that can degrade hyaluronan. This thesis examines the properties of hyaluronidase-1 and hyaluronidase-3, as well as the production of hyaluronan and the expression of hyaluronidase and hyaluronan synthases in mineralising osteoblasts. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1278426 / Thesis (Ph.D.) -- University of Adelaide, School of Medicine, 2007

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / The mammalian hyaluronidases represent a family of enzymes that can degrade hyaluronan. This thesis examines the properties of hyaluronidase-1 and hyaluronidase-3, as well as the production of hyaluronan and the expression of hyaluronidase and hyaluronan synthases in mineralising osteoblasts. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1278426 / Thesis (Ph.D.) -- University of Adelaide, School of Medicine, 2007

Thesis (Ph.D.)--Aberdeen University, 2009. / Title from web page (viewed on Jan. 5, 2010). Includes bibliographical references.

No description available.

Bisphosphonates are the most widely prescribed anti-resorptive agents and work by preventing the post-translational modification (prenylation) of small GTPases in osteoclasts, subsequently leading to cell death by apoptosis.  Phosphonocarboxylate analogues of bisphosphonates also have anti-resorptive activity and work by inhibiting the enzyme Rab GGTase, thereby preventing the prenylation of Rab GTPases specifically.  Rab GTPases comprise a large family of related proteins that coordinate vesicular trafficking, which involves the processing, transportation and delivery of cellular cargo in a strict temporal and spatial manner.  In osteoclasts, vesicular trafficking is vital for the formation of the ruffled border (the resorptive organelle of the cell), the delivery of lytic enzymes and acid into the resorption space, and the uptake and disposal of bone degradation products.  However, the role that specific Rabs play in this functionally unique cell type remains poorly defined, and the Rab expression profile in osteoclasts is incomplete.  The work presented here aimed to increase our understanding of the role that Rabs play in osteoclasts.  Results indicate that the 70% reduction of Rab GGTase activity observed in <i>gunmetal </i>mice is detrimental to the activity of osteoclasts and osteoblasts <i>in vitro</i>, therefore highlighting the importance of Rabs for bone resorption and deposition.  Furthermore, this study is the first to determine the Rab expression profile of human osteoclasts, following a proteomic approach, and describes the transfection methods devised to characterise these candidate Rabs in osteoclasts.  Finally, this study details the characterisation of Rab18 in human osteoclasts, following its discovery during proteomic analysis.

Thesis (Ph. D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xvii, 292 p.; also includes graphics (some col.). Includes abstract and vita. Advisor: Mihael C. Ostrowski, Dept. of Molecular, Cellular and Developmental Biology. Includes bibliographical references (p. 259-292).