Call number: LD2668 .T4 1979 E37 / Master of Science

Call number: LD2668 .T4 1979 G45 / Master of Science

Orientadores: Marcio de Moraes, Luciana Asprino / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-09-11T21:11:20Z (GMT). No. of bitstreams: 1 Stang_Bento_M.pdf: 1339091 bytes, checksum: e0465aaac7a4efde689e4fdfd75635e9 (MD5) Previous issue date: 2007 / Resumo: O objetivo deste estudo foi avaliar retrospectivamente os casos de cisto ósseo traumático (COT) tratados pela Área de Cirurgia Buco-Maxilo-Facial da Faculdade de Odontologia de Piracicaba da Universidade Estadual de Campinas, no período de janeiro de 1999 a dezembro de 2006, sendo avaliados 8.383 prontuários, dos quais 11 pacientes apresentaram COT. Não houve prevalência por gênero e os pacientes da cor branca foram envolvidos com maior freqüência (64%). Todos os casos acometeram indivíduos na segunda década de vida, com idade média de 15,2 anos. A mandíbula esteve envolvida em 100% dos casos, acometendo com maior freqüência a região de sínfise mandibular (55%), seguida pela região de corpo (36%) e ramo mandibular (9%). Cinco pacientes relataram história de trauma, ocorrido entre dois a nove anos anteriores ao diagnóstico da lesão. Todos os pacientes foram submetidos à biópsia incisional, associada à exploração cirúrgica da cavidade cística, que já representou a forma de tratamento. O tempo médio de proservação foi de 35,7 meses, o menor e o maior período de acompanhamento foram de 7 e 85 meses respectivamente, período no qual não foi observada recidiva. O tratamento através da cirurgia exploratória, realizado no momento da biópsia, foi efetivo em todos os casos deste estudo. Os resultados deste estudo contribuíram com a caracterização do COT e nos permitiram concluir que: Podemos sugerir que a etiologia do COT deve ter como suspeita o trauma, não havendo relação causal com gênero ou raça; O COT é uma lesão rara, que acomete preferencialmente a mandíbula de pacientes na segunda década de vida; A descoberta do COT ocorre em exames de rotina, sem que a lesão seja a queixa do paciente; O tratamento do COT através da exploração cirúrgica mostrou-se efetivo e seguro / Abstract: The aim of this study was retrospectively evaluate the cases of traumatic bone cyst (TBC) treated by the Oral and Maxillofacial Surgery Area of Piracicaba Dental School - Campinas State University, between January of 1999 to December of 2006, being evaluated 8,383 clinical handbooks, of which 11 patients had presented TBC. There was no prevalence of the gender and Caucasian patients were involved with more frequency (64%). All the cases occurred in patients that were in the second decade of life with a mean age of 15.2 years. Mandible was involved in 100% of the cases, with a prevalence on the symphisis (55%), followed by mandibular body (36%) and mandibular ramus (9%). Five patients reported trauma history, occurred between two to nine previous to the diagnosis of the lesion. All the patients were submitted to an incisional biopsy associate to the surgical exploration of the cystic socket. All patients were submitted to an incisional biopsy that represented the definitive treatment. The average time of preservation was of 35,7 months, the minor and the biggest period of accompaniment had been of 7 and 85 months respectively, period in which was not observed recurrence. The treatment through exploratory surgery, carried through at the moment of the biopsy, was effective in all the cases of this study. The results of this study had contributed with the characterization of the TBC and they had allowed us to conclude that: The etiology of the TBC must have trauma as suspected, with no causal relationship gender or race; The TBC is a rare injury, that have preferential incidence the inferior jaw of patients in the second decade of life; The discovery of TBC occurs in routine examinations, the injury is not the complaint of the patient; The treatment of the COT through the surgical exploration revealed effective and safe / Mestrado / Cirurgia e Traumatologia Buco-Maxilo-Faciais / Mestre em Clínica Odontológica

published_or_final_version / Dental Surgery / Master / Master of Dental Surgery

Orientador: Renato Mazzonetto / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-15T01:14:41Z (GMT). No. of bitstreams: 1 RodriguezChessa_JaimeGiuseppe_D.pdf: 2629539 bytes, checksum: 9e5185fe3e354d64d0557af2bfec5348 (MD5) Previous issue date: 2009 / Resumo: A utilização de fixações zigomáticas e implantes convencionais para reabilitação de maxilas atróficas constitui uma alternativa recente e vem sendo amplamente descrito na literatura científica. Essas fixações foram desenvolvidas inicialmente para reabilitar pacientes que apresentavam seqüelas devido à ressecção de tumores sendo, atualmente, utilizados também como terapia para pacientes com reabsorção de osso alveolar em região posterior. O objetivo deste estudo foi avaliar retrospectivamente as fixações zigomáticas realizadas na área de Cirurgia Buco- Maxilo-Facial da Faculdade de Odontologia de Piracicaba da Universidade Estadual de Campinas (FOP-UNICAMP) e da área de Implantologia da Faculdade de Odontologia da Universidade de Guarulhos (FO-UnG) no período de Junho de 2006 a Julho de 2009. Para a realização deste estudo foram avaliados 29 pacientes submetidos à instalação de fixações zigomáticas. O tempo médio de proservação foi de 20 meses. Os dados obtidos nos prontuários clínicos foram transcritos para uma ficha de coleta de dados especificamente desenvolvida para esta pesquisa e transferidos periodicamente para serem analisados. Desse total, 18(62%) pacientes eram do gênero feminino e 11(38%) do gênero masculino. Das 67 fixações zigomáticas e 70 implantes convencionais instalados nos pacientes, 10 pacientes foram submetidos a carga imediata (34,48%) e 19 pacientes a carga tardia (65,51%) podendo se observar um índice de perda de 7,46% para os casos de carga imediata e 13,43% para os casos de carga tardia. Em relação a motivação para a instalação das fixações zigomáticas, o principal fator foi a queixa funcional, presente em 22 pacientes (75,86%), a queixa estética para 2 pacientes (6,9%) e a queixa funcional-estética em 5 pacientes (17,24%). A indicação principal foram os casos de reabsorção alveolar severa em 21 casos (72,41%), seguido pelos insucessos na reconstrução com enxerto ósseos intraorais e extraorais com 4 (13,79%),e 1 caso (3,45%), respectivamente. A reabilitação mais utilizada foi acomposta por duas fixações zigomáticas associadas a quatro implantes convencionais (44,82% dos casos), seguido pelo uso de 4 implantes zigomáticos (17,24%) e 2 fixações zigomáticos associadas a 2 implantes convencionais (13,79%). Em relação às complicações a principal foi a perda da osseointegração (40%), seguido por mucosite (20%) e a dor persistente (15%), sinusite (15%) assim como a exteriorização das fixações (10%). Quanto à perda das fixações zigomáticos, dos 67 implantes inicialmente colocados, 14 foram perdidos perfazendo uma taxa de insucesso de 20,90%. Foi realizada uma avaliação por meio de uma escala visual analógica (EVA) obtendo-se um nível de satisfação aceitável, a cirurgia foi considerada como pouco traumatizante e com melhora significativa da função e estética. / Abstract: The use of zygomatic implants and conventional implants for rehabilitation of atrophic jaws is a recent alternative and has been widely described in scientific literature. These fixtures were initially developed to rehabilitate patients that showed sequelae after tumors resectionts. There also used as a therapy for patients with resorption of the alveolar bone in the posterior region. The aim of this study was to evaluate retrospectively the zygomatic implants performed in the Division of Oral and Maxillo-Facial Surgery, Piracicaba Dental School, Campinas State University (FOP-UNICAMP) and the division Implantology, Dental School, University of Guarulhos (FO-UnG) between June 2006 to July 2009. 29 patients where evaluated for this study subjected to the installation of zygomatic implants. The mean proservation was 20 months. Data from clinical records were stored in a form of data collection specifically designed for this research and transferred periodically to be analyzed. From this total, 18 (62%) patients were female and 11 (38%) were male. Of the 67 zygomatic implants and 70 conventional implants installed in patients, 10 patients underwent immediate loading (34.48%) and 19 patients a late charge (65.51%) can be observed a loss rate of 7.46% for cases of immediate loading and 13.43% for cases of late charge. Regarding the motivation for the installation of zygomatic implants, the main factor was the functional complaint, present in 22 patients (75.86%), the complaint aesthetics for 2 patients (6.9%) and functional-aesthetic complaint in 5 patients (17.24%). The main indication was the case of severe alveolar resorption in 21 cases (72.41%), followed by failures in reconstruction with intraoral bone graft and extraoral with 4 (13.79%), and 1 case (3.45%) respectively. The most rehabilitation technique consist of two zygomatic implants associated with four conventional implants (44.82% of cases), followed by the use of 4 zygomatic implants (17.24%) and 2 zygomatic fixations associated with 2 conventional implants (13, 79%). Regarding the main complication was the lossof osseointegration (40%), followed by mucositis (20%) and persistent pain (15%), sinusitis (15%) as well as the manifestation of fixations (10%). Regarding the loss of the zygomatic fixations, of the 67 implants originally placed 14 were lost resulting in a failure rate of 20.90%. An evaluation was performed by a visual analogue scale (VAS) and obtained an acceptable level of satisfaction, surgery was considered as low level of trauma and significant improvement in function and aesthetics. / Doutorado / Cirurgia e Traumatologia Buco-Maxilo-Faciais / Doutor em Clínica Odontológica

published_or_final_version / Dentistry / Doctoral / Doctor of Philosophy

Bone loss from the cranio-maxillofacial region is a major clinical problem affecting patients worldwide. Conventional treatment strategy includes the use of autogenous or allogeneic bone, biomaterials, and osteogenic growth factors. However, there has been no effective therapy for most cases so far. Stem cell-based gene therapy is the latest research method with possible applications in humans. The present study aims to (1) characterize rabbit mesenchymal stem cells (MSCs) relating to growth pattern, surface antigens, and the potential for multi-differentiation; (2) determine the transduction efficiency and duration of recombinant adeno-associated virus2 carrying enhanced green fluorescent protein (rAAV2EGFP) reporter gene in rabbit MSCs and study the effects of rAAV2EGFP transduction on stem cells’ phenotype and capacity of multi-differentiation; (3) evaluate the differentiation characteristics of rabbit MSCs following recombinant adeno-associated virus 2 carrying bone morphogenetic protein 2 gene (rAAV2BMP2) transduction; (4) investigate whether MSCs transduced by rAAV2BMP2 could successfully induce bone regeneration in rabbit critical-size cranial defects. MSCs were isolated from bone marrows of rabbit tibias and cultured. Cell counting and colony-forming assays demonstrated that growth rates of MSCs dropped substantially with increasing passages. Flow cytometry on MSCs at passage 1 showed that cells expressed high level of CD49a and low level of CD44 as well as stage-specific embryonic antigen 4 (SSEA4). Multi-differentiation and reverse transcriptase-polymerase chain reaction (RTPCR) tests demonstrated that rabbit MSCs were capable to differentiate into osteocytes, chondrocytes and adipocytes. Immunofluorescence microscopy showed that rabbit MSCs produced a series of hematopoietic growth factors, including stem cell factor (SCF), vascular endothelial growth factor-A (VEGFA) and granulocyte macrophage colony-stimulating factor (GMCSF). Subsequently, rabbit MSCs were transduced with rAAV2EGFP in vitro. By comparing the transduction efficiency with different doses of rAAV2EGFP particles, multiplicity of infection (MOI) of 1 x 10 4 was identified as an optimal parameter for the transduction of rAAV2 in rabbit MSCs. Fluorescent microscopy demonstrated long-term expression of EGFP in rabbit MSCs after transduction both in vitro and in vivo. In addition, cell proliferation assay, adipogenic induction test and flow cytometry showed that rAAV2EGFP transduced MSCs exhibited a similar pattern with non-transduced cells on the cell growth, capacity of adipogenic differentiation and expression of surface antigens, indicating that rabbit MSCs maintain their stem cell properties after rAAV2EGFP transduction. / published_or_final_version / Dentistry / Doctoral / Doctor of Philosophy

Bibliography: leaves 187-211. / 288 leaves : col. ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / An observational retrospective study to determine if any difference exists in the soft tissue profile of Class II division 1 patients before and after treatment with three different functional appliances; the activator with headgear, the Clark Twin Block, and the Frankel. / Thesis (M.D.S.)--University of Adelaide, Dept. of Dentistry, 1999

Bibliography: leaves 187-211. / 288 leaves : col. ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / An observational retrospective study to determine if any difference exists in the soft tissue profile of Class II division 1 patients before and after treatment with three different functional appliances; the activator with headgear, the Clark Twin Block, and the Frankel. / Thesis (M.D.S.)--University of Adelaide, Dept. of Dentistry, 1999