Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-04-19T13:40:36Z No. of bitstreams: 1 Larice Bruna Ferreira Soares.pdf: 837200 bytes, checksum: 4403ed208b78cc885e64ef4fc56f6f30 (MD5) / Made available in DSpace on 2017-04-19T13:40:36Z (GMT). No. of bitstreams: 1 Larice Bruna Ferreira Soares.pdf: 837200 bytes, checksum: 4403ed208b78cc885e64ef4fc56f6f30 (MD5) Previous issue date: 2017-02-07 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The objective of this study was to diagnose the epidemiological aspects of Bovine Viral Diarrhea (BVDV) and Bovine Infectious Rhinotracheitis virus (IBR) in buffaloes in the state of Pernambuco, Brazil. A total of 244 buffalo blood samples collected from the municipalities of Agrestina (n = 5), Água Preta (n = 50), Canhotinho (n = 21), Quipapá (n = 5), Ribeirão (113) And Rio Formoso (n = 50). The search for anti-BVDV and anti-BoHV-1 antibodies was performed by the virus neutralization (VN) technique. To analyze the association between the serological status for BoHV-1 infection and the aspects of hygienic-sanitary and reproductive management, an investigative questionnaire was applied with objective questions regarding the general characteristics of the property, productive, reproductive and sanitary management. An occurrence of 97.9% (239/244) of anti-BVDV antibodies and 56.1% (137/244) anti-BoHV-1 was observed. Co-infection was observed in 55.3% (135/244) of the animals. It was observed that 100.0% of the properties showed at least one animal positive for both infections. The distribution of antibodies to buffaloes by properties varied from 90.5% to 100.0% for BVDV and from 4.8% to 100.0% for BoHV-1. It was not possible to perform the association analysis for BVDV infection; however, for the BoHV-1 infection, the variables that presented a significant association were: extensive breeding system (P <0.001); Open herd (P = 0.029); Absence of reproductive rest (P = 0.029); Natural mating in females with reproductive problems (P <0.001); Type of exploration (P = 0.0014); Presence of wild animals (P <0.001) and absence of cleaning of the facilities (P = 0.008). It is concluded that Bovine Viral Diarrhea and Bovine Infectious Rhinotracheitis virus infections occur in buffaloes in the state of Pernambuco. Thus, it is suggested that prophylaxis measures such as routine diagnosis, animal reproductive control, and strict hygienic-sanitary care should be implemented in the properties in order to reduce the reproductive losses caused by these infections. / Objetivou-se com este estudo diagnosticar os aspectos epidemiológicos da infecção pelo vírus da Diarreia Viral Bovina (BVDV) e Rinotraqueíte Infecciosa Bovina (IBR) em bubalinos no estado de Pernambuco, Brasil. Foram coletas de 244 amostras sanguíneas de búfalos procedentes dos municípios de Agrestina (n=5), Água Preta (n=50), Canhotinho (n=21), Quipapá (n=5), Ribeirão (n=113) e Rio Formoso (n=50). A pesquisa de anticorpos anti-BVDV e anti-BoHV-1 foi realizada pela técnica de virusneutralização (VN). Para análise de associação entre o status sorológico para infecção pelo BoHV-1 e os aspectos de manejo higiênico-sanitário e reprodutivo aplicou-se um questionário investigativo com perguntas objetivas, referentes às características gerais da propriedade, manejo produtivo, reprodutivo e sanitário. Observou-se uma ocorrência de 97,9% (239/244) de anticorpos anti-BVDV e 56,1% (137/244) anti-BoHV-1. Constatou-se co-infecção em 55,3% (135/244) dos animais. Observou-se que 100,0% das propriedades apresentaram pelo menos um animal positivo para as duas infecções. A distribuição da ocorrência de anticorpos em búfalos por propriedades variou de 90,5% a 100,0% para BVDV e de 4,8% a 100,0% para BoHV-1. Não foi possível realizar a análise de associação para à infecção pelo BVDV, entretanto, para à infecção pelo BoHV-1, as variáveis que apresentaram associação significativa foram: sistema de criação extensivo (P<0,001); rebanho aberto (P=0,029); ausência de descanso reprodutivo (P=0,029); monta natural em fêmeas com problemas reprodutivos (P<0,001); tipo de exploração (P=0,0014); presença de animais silvestres (P< 0,001) e ausência de limpeza das instalações (P=0,008). Conclui-se que as infecções pelo vírus da Diarreia Viral Bovina e Rinotraqueíte Infecciosa Bovina ocorrem em búfalos no estado de Pernambuco. Desta forma, sugere-se que medidas de profilaxia como o diagnóstico rotineiro, controle reprodutivo dos animais, e cuidados higiênicos-sanitários rigorosos devem ser implementados nas propriedades com o intuito de diminuir as perdas reprodutivas ocasionadas por estas infecções.

Assessment of occurrence and diagnostic methods of viral diseases in cattle – viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), rabies, enzootic bovine leukosis (EBL), and spongiform encephalopathy (BSE) – was carried out for the first time in Lithuania. It was established that viruses of rabies, infectious rhinotraheitis and viral diarrhoea are most widespread in the country. It was determined that occurrence of rabies in cattle is parallel with the infection of wildlife with rabies virus. Analysis of eradication programme of enzootic bovine leucosis was done. It revealed that only combined application of diagnostic and preventive measures allowed reducing the cattle infection up to 0.2%. Though bovine spongiform encephalopathy has not been recorded in Lithuania, it is feasible to implement its diagnostic and prevention programme. An overall financial analysis of expenditures on BSE and viral diseases diagnostics and control was for the first time done in Lithuania. It showed that BSE and EBL occupied the leading positions in the structure of expenditures on viral diseases. In 2001, expenditures on BSE investigations accounted for 76.68% and in 2004 for 86.74% of the total. Expenditures on EBL investigations relatively reduced from 86.98% in 2000 to 8.47% in 2004. During the time under consideration, expenditures on investigations of other viral diseases changed but little. It was determined that consistent and wide-scale preventive vaccination created... [to full text]