Submitted by (lucia.rodrigues@ufrpe.br) on 2016-08-10T13:48:00Z No. of bitstreams: 1 Maria de Fatima da Silva.pdf: 302537 bytes, checksum: 7c6d2b448ec2b8c92bc437c2f3c61538 (MD5) / Made available in DSpace on 2016-08-10T13:48:00Z (GMT). No. of bitstreams: 1 Maria de Fatima da Silva.pdf: 302537 bytes, checksum: 7c6d2b448ec2b8c92bc437c2f3c61538 (MD5) Previous issue date: 2007-02-27 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Peanut (Arachis hypogaea L.) Is legume used in the world with a high percentage of comestible oil and protein and in Brazil is cropped in different soil and climatic conditions. Due to its adaptability to tropical conditions and with high economic value peanut is very important for typically small, family based properties involved in food production in the Northeastern Brazilian. With the objective to evaluate the effectiveness of peanut inoculation with selected strains of Bradyrhizobium spp. bacteria it was carried out a field experiment in not irrigated conditions, in a soil located at the Itapirema Experimental Station (IPA), tropical rainforest zone of Pernambuco state. The experiment was conducted in a factorial 2 x10, in randomized block design, with four replicates. Were used two peanut cultivars (BR 1 and BRS Havana) comparing 8 peanut strains, including the controls treatments with N fertilization as ammonium sulphate (200 kg N ha-1) and without nitrogen fertilizers. In the periods of flowering and grain maturity plants were collected (6 per plot) for evaluation of nitrogen fixation (dry nodules biomass, dry plant biomass, total N in shoots and relative efficiency of strains). At the final of the experiment were determined the following components of plant productivity: dry biomass of 100 grains(g); dry biomass of 100 pods (g); grain and pod yields (kg ha-1). Nitrogen fertilization increased dry biomass and total N accumulation in shoots in the period of grain maturation (p≤ 0,05). The relative efficiency of the strains was greater on cultivar BRS Havana. Bradyrhizobium spp. inoculation showed no significant difference (p≤0,05) to the others treatments in reference to dry biomass of nodules. It was not observed significant difference between treatments on grain and pod yields. In the soil used the rhizobia native from soil was so effective than rhizobia applied by inoculation and they supply nitrogen to attend the N demand by peanut cropped inthe used Brazilian rainforest soil. / O amendoim (Arachis hypogaea L.) é uma oleaginosa consumida mundialmente. No Brasil é cultivada nas mais diferentes condições edafoclimáticas. Devido sua boa adaptabilidade às condições tropicais e por ser uma cultura de valor econômico, o amendoim pode ter um papel importante para a capitalização de pequenos agricultores na região Nordeste. Com o objetivo de avaliar a efetividade da inoculação com Bradyrhizobium spp. em solo da Zona de Mata de Pernambuco na cultura do amendoim, foi realizado um experimento no campo, em condições de sequeiro, na Estação Experimental de Itapirema (IPA), Goiana, Pernambuco. O delineamento experimental adotado foi o de blocos casualizados, com 4 repetições, no arranjo fatorial 2 X 10. Foram usadas duas cultivares de amendoim (BR 1 e BRS Havana) e 10 tratamentos como fontes de nitrogênio, 8 tratamentos com inoculação com estirpes de Bradyrhizobium; 1 tratamento sem inoculação e com fertilizante nitrogenado, aplicado na forma de Sulfato de amônio (200 kg ha-1 de N); e 1tratamento sem inoculação e sem fertilizante com N. Nos períodos de florescimento e de maturação dos frutos 6 plantas por parcela foram coletadas ao acaso, para avaliação da fixação biológica do nitrogênio (biomassa seca de nódulos, biomassa seca da parte aérea, N total acumulado na parte aérea e eficiência relativa dasestirpes). No final do ciclo foram determinados: biomassa seca de 100 grãos (g);biomassa seca de 100 vagens (g), rendimento de grãos (kg ha-1) e rendimento de vagens (kg ha-1). Houve diferença significativa (p≤ 0,05) para acúmulo de biomassa seca e de N da parte aérea no período de maturação dos frutos, onde o tratamento com N fertilizante foi superior aos demais. A eficiência das estirpes foi mais acentuada na cultivar BRS Havana. Não houve diferença significativa (p≤ 0,05) entre os tratamentos para biomassa seca dos nódulos e para o rendimento de vagens e de grãos. Nas condições em que o experimento foi desenvolvido a população rizobiana nativa foi eficiente no suprimento de nitrogênio para atender a demanda deste nutriente pela cultura do amendoim.

Soil samples from different locations with varied soybean cultivation histories were taken from arable fields in 2018 in East Germany and Poland (Lower Silesia) to evaluate the specific microsymbionts of the soybean, Bradyrhizobium japonicum, one to seven years after inoculation. Soybeans were grown in the selected farms between 2011 and 2017. The aim of the experiment was to investigate whether there is a difference in rhizobia contents in soils in which soybeans have been recultivated after one to seven years break, and whether this could lead to differences in soybean plant growth. The obtained soil samples were directly transferred into containers, then sterilized soybean seeds were sown into pots in the greenhouse. After 94 days of growth, the plants were harvested and various parameters such as the nodular mass, number of nodules, and dry matter in the individual plant parts were determined. In addition, the relative abundances of Bradyrhizobium sp. in soil samples were identified by sequencing. No major decline in Bradyrhizobium sp. concentration could be observed due to a longer interruption of soybean cultivation. Soil properties such as pH, P, and Mg contents did not show a significant influence on the nodule mass or number, but seem to have an influence on the relative abundance of Bradyrhizobium sp. The investigations have shown that Bradyrhizobium japonicum persists in arable soils even under Central European site conditions and enters into an effective symbiosis with soybeans for up to seven years.:Introduction Materials and Methods Results Discussion Supplementary Materials Author Contributions Funding Acknowledgments Conflicts of Interest References