Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.

by Wong Ping Shing. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 97-112). / ACKNOWLEDGMENTS --- p.i / ABSTRACT --- p.ii / ABBREVIATIONS --- p.viii / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- General properties of zinc / Chapter 1.1.1 --- Biochemistry of zinc --- p.2 / Chapter 1.1.2 --- Distribution of zinc in body --- p.3 / Chapter 1.1.3 --- Roles of zinc in protein function --- p.4 / Chapter 1.2 --- Zinc and zinc-binding proteins in brain / Chapter 1.2.1 --- Distribution of zinc in brain --- p.7 / Chapter 1.2.2 --- Metabolism of zinc in brain --- p.9 / Chapter 1.2.3 --- Compartments of zinc in brain --- p.10 / Chapter 1.2.4 --- Zinc-binding proteins in brain --- p.12 / Chapter 1.3 --- Pathological conditions of brain in relation to zinc --- p.15 / Chapter 1.4 --- Aim of the project --- p.20 / Chapter 2. --- MATERIALS AND METHODS --- p.22 / Chapter 2.1 --- Detection of zinc-binding proteins / Chapter 2.1.1 --- Sodium-Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.22 / Chapter 2.1.2 --- Electroblotting --- p.24 / Chapter 2.1.3 --- Radioactive zinc blotting --- p.25 / Chapter 2.1.4 --- Autoradiography --- p.25 / Chapter 2.2 --- Subcellular fractionation of porcine brain --- p.26 / Chapter 2.3 --- Purification and structural characterization of a 19 kDa zinc-binding protein / Chapter 2.3.1 --- Purification of a 19 kDa protein --- p.27 / Chapter 2.3.2 --- Sequencing of N-terminal blocked 19 kDa protein --- p.30 / Chapter 2.4 --- Characterization of the binding and biological properties of the 19 kDa zinc-binding protein / Chapter 2.4.1 --- Effect of divalent metal ions on zinc binding to the 19 kDa protein --- p.33 / Chapter 2.4.2 --- Effect of pH on the dissociation of radioactive zinc from the19 kDa protein --- p.34 / Chapter 2.4.3 --- Radioactive calcium blotting --- p.34 / Chapter 2.4.4 --- Interaction of radioactive zinc and radioactive calcium binding to the 19 kDa protein --- p.35 / Chapter 2.4.5 --- Calmodulin activity assay --- p.35 / Chapter 3. --- RESULTS / Chapter 3.1 --- Specificity of radioactive zinc-blot on zinc-binding protein detection --- p.38 / Chapter 3.2 --- Zinc-binding proteins in porcine brain --- p.38 / Chapter 3.3 --- Purification and identification of a cytosolic 19 kDa zinc- binding protein in porcine brain / Chapter 3.3.1 --- Zinc-dependent hydrophobic interaction chromatography --- p.44 / Chapter 3.3.2 --- N-terminal amino acid sequencing --- p.51 / Chapter 3.3.3 --- High pH native gel electrophoresis of 19 kDa protein --- p.51 / Chapter 3.4 --- The zinc and calcium binding properties of the 19 kDa protein / Chapter 3.4.1 --- Effect of pre-exposure to divalent cations on zinc binding --- p.54 / Chapter 3.4.2 --- Competition by divalent cations for zinc binding --- p.56 / Chapter 3.4.3 --- pH dependency of zinc dissociation --- p.56 / Chapter 3.4.4 --- Effect of zinc on radioactive calcium binding --- p.61 / Chapter 3.5 --- The biological activity of the 19 kDa protein / Chapter 3.5.1 --- Effect of the 19 kDa protein on the activity of calmodulin- dependent phosphodiesterase --- p.66 / Chapter 3.5.2 --- Effect of zinc on calmodulin-dependent phosphodiesterase activity --- p.69 / Chapter 3.5.4 --- "Effect of zinc on calcium-deficient, calmodulin-dependent phosphodiesterase activity" --- p.72 / Chapter 4. --- DISCUSSION / Chapter 4.1 --- Detection and Purification of zinc-binding proteins / Chapter 4.1.1 --- Strategy for the detection of zinc-binding proteins --- p.77 / Chapter 4.1.2 --- Purification of zinc-binding protein --- p.79 / Chapter 4.2 --- Amino acid sequencing of the 19 kDa protein --- p.82 / Chapter 4.3 --- Binding properties of the 19 kDa zinc-binding protein --- p.86 / Chapter 4.4 --- Effect of zinc and 19 kDa zinc-binding protein on calmodulin dependent phosphodiesterase --- p.92 / Chapter 4.5 --- Effect of zinc on the properties of calmodulin --- p.90 / Chapter 4.6 --- Significance of the ability of zinc to affect calmodulin activity --- p.94 / Chapter 5. --- CONCLUSION --- p.95 / Chapter 6. --- REFERENCES --- p.97

O estudo foi realizado através de avaliação de 214 hemisférios cerebrais, de 107 espécimes humanos, com a idade variando desde 12 semanas de gestação até 8 meses pós-natal. A idade gestacional dos fetos foi calculada através do seu peso corpóreo. Os fetos com malformações congênitas ou com encéfalos danificados foram excluídos. Após a fixação do encéfalo em solução de formol a 10%, foi removida a aracnóide para a análise dos sulcos do cérebro, os quais foram então estudados, desde o seu aparecimento, até sua formação completa. A principal finalidade desse estudo foi estabelecer os padrões de desenvolvimento morfológico dos sulcos cerebrais característicos de cada idade gestacional. Tendo como base a análise dos resultados, foram estabelecidas tabelas de referências cronológicas pertinentes à formação de cada sulco em toda superfície do cérebro. / The study was done through the analysis of 214 brain hemispheres of 107 human brains, with their ages ranging from 12 weeks of gestation to 8 months of postnatal life. The gestational age was calculated from their body weight. The fetuses with congenital abnormalities and or damaged brains were excluded from the study. After the brain fixation with 10% formalin, the arachnoid was removed for the study of the sulci and fissures of the brain, since their appearance until their complete development. The aim of this anatomical study was to establish a reliable sulci morphological pattern characteristic of each gestational age. Based in our findings, reference tables pertinent to the appearance of each sulci of all brain surface were built and are here presented.

O estudo foi realizado através de avaliação de 214 hemisférios cerebrais, de 107 espécimes humanos, com a idade variando desde 12 semanas de gestação até 8 meses pós-natal. A idade gestacional dos fetos foi calculada através do seu peso corpóreo. Os fetos com malformações congênitas ou com encéfalos danificados foram excluídos. Após a fixação do encéfalo em solução de formol a 10%, foi removida a aracnóide para a análise dos sulcos do cérebro, os quais foram então estudados, desde o seu aparecimento, até sua formação completa. A principal finalidade desse estudo foi estabelecer os padrões de desenvolvimento morfológico dos sulcos cerebrais característicos de cada idade gestacional. Tendo como base a análise dos resultados, foram estabelecidas tabelas de referências cronológicas pertinentes à formação de cada sulco em toda superfície do cérebro. / The study was done through the analysis of 214 brain hemispheres of 107 human brains, with their ages ranging from 12 weeks of gestation to 8 months of postnatal life. The gestational age was calculated from their body weight. The fetuses with congenital abnormalities and or damaged brains were excluded from the study. After the brain fixation with 10% formalin, the arachnoid was removed for the study of the sulci and fissures of the brain, since their appearance until their complete development. The aim of this anatomical study was to establish a reliable sulci morphological pattern characteristic of each gestational age. Based in our findings, reference tables pertinent to the appearance of each sulci of all brain surface were built and are here presented.