Graduation date: 2003

Depuis les années 1990, les efflorescences de cyanobactéries se sont intensifiées durant une partie de l'été et de l'automne au lac Brome (Cantons de l'Est, Québec). Malheureusement, ces proliférations cyanobactériennes peuvent s'accompagner d'une production et d'une libération de toxines dans les écosystèmes affectés. Cependant, les conditions qui favorisent la formation de ces fleurs d'eau ne sont pas encore complètement clarifiées. Les objectifs principaux de cette étude était de montrer: 1/ le lien étroit qui existe entre les cellules de résistances présentent à la surface des sédiments et la formation des efflorescences pélagiques; 2/ utiliser les akinètes et les pigments fossiles comme « proxie » pour retracer l'historique des cyanobactéries depuis les premières années d'installation des colons dans la région d'étude; 3/ tenter de relier les fluctuations de l'abondance relative des cyanobactéries ayant la capacité de fixer l'azote atmosphérique (N₂), avec la variation relative des concentrations en éléments nutritifs (N et P), résultant en partie de l'eutrophisation de l'écosystème. Les résultats obtenus confirment qu'il y a présentement un problème de qualité de l'eau au lac Brome, lié à un développement massif de cyanobactéries potentiellement toxiques pendant la saison libre des glaces. Ce lac présente une succession d'efflorescences de cyanobactéries caractéristiques d'un milieu eutrophe, ayant la capacité de produire des akinètes et de fixer le N₂ (Anabaena sp. et Aphanizomenon sp.). Nous avons obtenu une relation significative entre l'abondance relative des akinètes dans les sédiments et la biomasse estimée des cyanobactéries grâce aux pigments fossiles (Canthaxanthine-akinètes : R² = 0.43, p=0,0016; Aphanizophyll-akinètes: R² = 0.71, p<0,0001l). Par ailleurs, cette étude suggère que la zone littorale tout comme la zone profonde peuvent contribuer à l'ensemencement de la colonne d'eau. Enfin, nous avons obtenu une concordance assez nette entre l'augmentation de l'abondance relative des cyanobactéries ayant la capacité de fixer le N₂, la diminution du ratio N/P (qui passe de 30 dans les années 1970 à 10 en 2005) et la diminution du ratio ¹⁵N/¹⁴N reflétant un accroissement des taux de fixation du N₂ dans l'écosystème. Le recoupement entre ces données suggère que les cyanobactéries ont en partie réussi à dominer l'assemblage phytoplanctonique grâce à leur capacité de fixer le N₂. ______________________________________________________________________________ MOTS-CLÉS DE L’AUTEUR : Cyanobactéries, Paléopigments, Akinètes, Eutrophisation.

Forage growth in the subarctic is sub-optimal due to low soil nutrient levels. Forage crops in the Yukon Territory consistently require nitrogen (N) and phosphorus fertilization to meet plant requirements. Fertilization is expensive due to transportation costs and potentially harmful to the environment so alternative, more sustainable, sources of nutrients are being sought. Alfalfa is an alternative, but there is limited knowledge in the Yukon of the benefits and management of this crop as a replacement for fertilizer N. Experiments were carried out in south central Yukon during the 2005 and 2006 field seasons to examine the potential of co-inoculation of alfalfa with N-fixing Ensifer meliloti and phosphate-solubilizing Penicillium bilaii to increase the dry matter yield and N fixation of monoculture alfalfa (Medicago sativa) cv Peace and binary mixed alfalfa with smooth bromegrass (Bromus inermis) cv Carlton or timothy (Phleum pratense) cv Climax forage swards. Interactions between alfalfa inoculation and N fertilization and late season harvest treatments were assessed. The TagTeam® inoculant from Philom Bios was used as the rhizobium source which contains both Ensifer meliloti isolate NRG-34 and Penicillium bilaii isolate PB-50. Nitrogen fixation was determined using the total plant N difference method. Alfalfa growth and nodulation was successful in the trials. Inoculation had a positive impact on N fixation, whereas urea fertilizer at 25 kg N/ha had a negative impact in most cases. In the mixed alfalfa and smooth bromegrass stand there was a positive contribution from the alfalfa in both the establishment and second year with N fixation rates of up to 14 kg/ha. In the mixed timothy and alfalfa stand the N fixation reached 35 kg/ha in the establishment year and 102 kg/ha in the second year. In the establishment year the dry matter yield and N fixation of the TagTeam® inoculated, monoculture alfalfa plots were 3.1 t/ha and 77 kg N/ha. In the second year, the unharvested inoculated alfalfa treatment yielded 3.4 t/ha with N fixation of 66 kg/ha compared to the late harvest treatment which yielded only 1.5 t/ha and an N fixation rate of 20 kg/ha. The effects of the late season harvest are startling and reflect the importance of removing grazing animals during the fall to allow plant energy reserves to accumulate in the roots. Fertilizer N replacement is possible with the seeding of alfalfa into existing hay stands or in monoculture.

Forage growth in the subarctic is sub-optimal due to low soil nutrient levels. Forage crops in the Yukon Territory consistently require nitrogen (N) and phosphorus fertilization to meet plant requirements. Fertilization is expensive due to transportation costs and potentially harmful to the environment so alternative, more sustainable, sources of nutrients are being sought. Alfalfa is an alternative, but there is limited knowledge in the Yukon of the benefits and management of this crop as a replacement for fertilizer N. Experiments were carried out in south central Yukon during the 2005 and 2006 field seasons to examine the potential of co-inoculation of alfalfa with N-fixing Ensifer meliloti and phosphate-solubilizing Penicillium bilaii to increase the dry matter yield and N fixation of monoculture alfalfa (Medicago sativa) cv Peace and binary mixed alfalfa with smooth bromegrass (Bromus inermis) cv Carlton or timothy (Phleum pratense) cv Climax forage swards. Interactions between alfalfa inoculation and N fertilization and late season harvest treatments were assessed. The TagTeam® inoculant from Philom Bios was used as the rhizobium source which contains both Ensifer meliloti isolate NRG-34 and Penicillium bilaii isolate PB-50. Nitrogen fixation was determined using the total plant N difference method. Alfalfa growth and nodulation was successful in the trials. Inoculation had a positive impact on N fixation, whereas urea fertilizer at 25 kg N/ha had a negative impact in most cases. In the mixed alfalfa and smooth bromegrass stand there was a positive contribution from the alfalfa in both the establishment and second year with N fixation rates of up to 14 kg/ha. In the mixed timothy and alfalfa stand the N fixation reached 35 kg/ha in the establishment year and 102 kg/ha in the second year. In the establishment year the dry matter yield and N fixation of the TagTeam® inoculated, monoculture alfalfa plots were 3.1 t/ha and 77 kg N/ha. In the second year, the unharvested inoculated alfalfa treatment yielded 3.4 t/ha with N fixation of 66 kg/ha compared to the late harvest treatment which yielded only 1.5 t/ha and an N fixation rate of 20 kg/ha. The effects of the late season harvest are startling and reflect the importance of removing grazing animals during the fall to allow plant energy reserves to accumulate in the roots. Fertilizer N replacement is possible with the seeding of alfalfa into existing hay stands or in monoculture.

Bibliography: leaves [274]-281 / vi, 281 leaves ; 31 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of English Language and Literature, 1992

Thesis (M.S.)--University of Nevada, Reno, 2007. / "August, 2007." Includes bibliographical references. Online version available on the World Wide Web.

Forage growth in the subarctic is sub-optimal due to low soil nutrient levels. Forage crops in the Yukon Territory consistently require nitrogen (N) and phosphorus fertilization to meet plant requirements. Fertilization is expensive due to transportation costs and potentially harmful to the environment so alternative, more sustainable, sources of nutrients are being sought. Alfalfa is an alternative, but there is limited knowledge in the Yukon of the benefits and management of this crop as a replacement for fertilizer N. Experiments were carried out in south central Yukon during the 2005 and 2006 field seasons to examine the potential of co-inoculation of alfalfa with N-fixing Ensifer meliloti and phosphate-solubilizing Penicillium bilaii to increase the dry matter yield and N fixation of monoculture alfalfa (Medicago sativa) cv Peace and binary mixed alfalfa with smooth bromegrass (Bromus inermis) cv Carlton or timothy (Phleum pratense) cv Climax forage swards. Interactions between alfalfa inoculation and N fertilization and late season harvest treatments were assessed. The TagTeam® inoculant from Philom Bios was used as the rhizobium source which contains both Ensifer meliloti isolate NRG-34 and Penicillium bilaii isolate PB-50. Nitrogen fixation was determined using the total plant N difference method. Alfalfa growth and nodulation was successful in the trials. Inoculation had a positive impact on N fixation, whereas urea fertilizer at 25 kg N/ha had a negative impact in most cases. In the mixed alfalfa and smooth bromegrass stand there was a positive contribution from the alfalfa in both the establishment and second year with N fixation rates of up to 14 kg/ha. In the mixed timothy and alfalfa stand the N fixation reached 35 kg/ha in the establishment year and 102 kg/ha in the second year. In the establishment year the dry matter yield and N fixation of the TagTeam® inoculated, monoculture alfalfa plots were 3.1 t/ha and 77 kg N/ha. In the second year, the unharvested inoculated alfalfa treatment yielded 3.4 t/ha with N fixation of 66 kg/ha compared to the late harvest treatment which yielded only 1.5 t/ha and an N fixation rate of 20 kg/ha. The effects of the late season harvest are startling and reflect the importance of removing grazing animals during the fall to allow plant energy reserves to accumulate in the roots. Fertilizer N replacement is possible with the seeding of alfalfa into existing hay stands or in monoculture. / Land and Food Systems, Faculty of / Graduate

Invasive weed species are a threat to the health and functionality of many rangeland systems. Downy brome (Bromus tectorum) is an invasive annual grass that affects the productivity of rangelands by decreasing the grazing capacity for livestock as well as altering the wildfire cycle and competing against more desirable vegetation for limited resources.In 2006, an Invasive Plant Management Plan and Environmental Assessment was approved for Dinosaur National Monument, calling for prioritization of invasive species management on high value wildlife habitat, vector areas, and for species with a high ecological impact. The Cub Creek Watershed was identified as a priority for immediate attention due to its high historical, recreational, and environmental significance.This research was another phase of an integrated effort to manage vegetation in the Cub Creek Watershed and surrounding rangelands. Field work at two locations within Dinosaur National Monument was coupled with greenhouse experiments to evaluate chemical and mechanical methods of downy brome seed reduction and control, and to evaluate the response of four weedy grasses to herbicides used in broadleaf weed management practices.

Bromus tectorum and other annual brome grasses have invaded many ecosystems of the western United States, and because of an annual-grass influenced alteration of the natural fire cycle on arid western range lands near monocultures are created and conditions in which the native vegetation cannot compete are established. Each year thousands of hectares become near monocultures of annual brome grasses. Pyrenophora semeniperda, a generalist seed pathogen of annual grasses, shows major potential as a possible mycoherbicide that could help in reducing the monocultures created by annual grasses. The purpose of this research was to identify the requirements for isolating cultures of P. semeniperda, search for a hypervirulent strain, and evaluate its effect in the field. The techniques for isolating the fungus have evolved and become more efficient. The first two years of working with P. semeniperda resulted in 11 isolates. During the third year of this study, we developed a single spore isolation technique that resulted in 480 additional isolates. Virulence screening resulted in detection of a range of isolate ability to kill non-dormant B. tectorum seeds. Ninety-two isolates represented a range of virulence from 0-44%. The variation in virulence was expressed mostly within populations rather than between populations. Similarly, virulence varied significantly within Internal Transcribed Spacer (ITS) genotypes and habitats but not between them. When conidial inoculum was applied in the field there was no observed difference in disease incidence between different levels of inoculum. This is thought to have been due to applying the inoculum under conditions in which most in situ seeds were infected and killed by already high field inoculum loads. While additional field trials are needed to optimize the inoculum effectiveness, the overall results of this research provide a good foundation for using P. semeniperda as a biological control for seed banks of annual brome grasses.

Thesis (M.S.)--Oregon State University, 2006. / Printout. Includes bibliographical references (leaves 27-31). Also available on the World Wide Web.