The brook trout <i>Salvelinus fontinalis</i> is the only salmonid native to the southern Appalachian Mountains, and is distributed across eastern North America from Canada to Georgia. This species was once abundant in coldwater lakes and streams throughout its range, but environmental disturbances and the introduction of non-native species have drastically reduced the number and sizes of wild populations. Genetic evidence suggests a division at the subspecies level between southern- and northern-derived brook trout populations, with the break between the two forms occurring roughly at the New River watershed. Before the subspecies structure was recognized, brook trout of northern origin were widely stocked throughout the Southeast. The primary objective of this study was to determine the phylogenetic origin of all wild brook trout populations in southwestern Virginia using allozyme markers. Seventy-eight streams believed to contain brook trout in the New, James, Holston, and Yadkin river drainages were sampled by backpack electrofishing. Muscle tissue samples were collected from 916 individuals from 56 populations using a non-lethal biopsy technique. The samples were analyzed by cellulose acetate gel electrophoresis and histochemical staining techniques. Variation at four polymorphic loci, including the diagnostic creatine kinase (<i>CK-A2*</i>) locus, was quantified in terms of genetic diversity and population genetic differentiation. Allele frequencies indicated that 19 populations were of putative southern origin, 5 of northern origin, and 32 of mixed genetic origin. The secondary objective was to determine the geographic distribution of southern- and northern-form brook trout populations throughout the native range using data compiled from all known genetic studies. A map of these data showed that the break between the southern and northern form is sharp, occurring at the New/Roanoke-James watershed divide. Populations from the New River drainage expressed the southern allele at a frequency of 85%, suggesting that their historic native character is southern, and that the presence of northern alleles is due to stocking or stream-capture events. The persistence of the southern form, despite the heavy stocking of northern-derived individuals, may be evidence of an adaptive advantage for the southern form of the species. Existence of adaptive genetic differentiation supports the case for conservation of the southern form of the species in future management of brook trout. / Master of Science

Barriers to fish movement can cause aquatic habitat fragmentation by reducing the amount of available habitat. The primary goal of my research was to investigate applications of population genetic analysis tools as indicators of barrier effects on brook trout populations in fragmented subwatersheds. In chapter1, I tested the hypothesis that brook trout population genetic differentiation (FST) above and below barriers will differ in relation to barrier height and gradient. I also tested the hypothesis that average gene diversity per locus (H) and the numbers of alleles (A) differed between samples below and above each barrier. There was no significant difference in average number of alleles (A) or average gene diversity per locus (H) between the above- and below-barrier samples, but linear regression identified a statistically significant relationship between barrier height and FST values. Unrooted neighbor-joining consensus trees of Cavalli-Sforza and Edwards (1967) chord distances provided evidence of genetic differentiation between samples of resident brook trout above and below natural barriers. Additionally, average total allelic diversity (A), average gene diversity per locus (H), average number of private alleles per locus per sample, and total alleles per sample differed between Level III Ecoregions. In chapter 2 I tested the hypothesis that the presence of a barrier, total habitat potentially isolated above a barrier (km), road density, and percent forest cover within a subwatershed (USGS 6th-level Hydrologic Units) were significant habitat fragmentation factors affecting the effective population size (Ne) of brook trout in the Blue Ridge Level III Ecoregion. Multivariable linear regression indicated that total habitat above the barrier (km) and road density were significant variables retained in the model to predict Ne. In chapter 3, the objective of the study was to infer relationships between barriers and family structure in brook trout populations. Maximum likelihood analysis of pairwise kinship relationships between above- and below-barrier individuals indicated the presence of parent-offspring relationships between above- and below-barrier individuals at six sites in the Blue Ridge Level III Ecoregion and five sites in the Northern Lakes and Forests Level III Ecoregion, which indicated movement of individuals between the above- and below-barrier locations. / Ph. D.

The relation between prey density and brook trout Salvelinus fontinalis food consumption has not been quantified in natural streams. This relation may be most critical in the summer when southern Appalachian trout have demonstrated poor growth. It is not Known whether the poor growth is due to the low food densities or to excess temperatures and increased metabolic costs. Purposes of this study were to quantify the relation between prey density and food consumption in brook trout, identify how diel feeding and prey size selectivity by brook trout affect the relation, and to determine if slow growth in the summer was caused by low daily rations or other factors. Daily consumption was estimated in May, July and September of 1989, for four streams in Shenandoah National Park. Sampling was performed at 6-hour intervals over 24 hours. Adult and yearling trout did not consume more food at one specific time throughout the summer, but juvenile trout (< 100 mm) fed significantly less at night in both July and September. All sizes of fish selected for prey longer than 4mm. The best predictor of daily ration (corrected for temperature) over the summer was the number of prey longer than 4mm/m³. Models parameterized to quantify the relation between prey density and food consumption showed that adult trout were significantly more likely to have a higher daily ration per gram body weight (temperature and size corrected) than yearling trout at low ( < 1.0 prey longer than 4mm/m³) prey densities. Daily ration of juvenile trout was independent of prey abundance. The prey density-consumption relation was more consistent within streams than among streams, which suggests that habitat or population characteristics may influence the prey density-consumption relation. Trout had the most energy available for growth in July followed by May and then September. Daily ration was most dependent on temperature in May and on prey density in July and September. Juvenile trout did not decrease daily ration as much from July to September as did yearling and adult trout. All sizes of trout met maintenance costs in both early and late summer, yet yearling and adult trout did not grow in late summer although stream temperature was optimum for growth. Growth was negatively correlated with daily ration in the late summer, suggesting that activity costs may be more important in determining growth in late summer than prey densities. Activity costs varied substantially among streams. The effect of food consumption and habitat on activity costs needs to be defined to improve understanding of the determinants of growth in stream environments. / Master of Science

Seasonal movements of anadromous brook trout (Salvelinus fontinalis) were studied in riviere au Saumon, James Bay, during 1986 to 1988. A comparison of the biology and energetic parameters between sea run and freshwater brook trout was made in the same system. Anadromous brook trout started their downstream movements in May and displacements peaked in June, but some also occurred in fall. Downstream movements were correlated with spring and fall freshets. Important interannual variation was observed in the movement pattern. Seaward movements of anadromous trout were limited to the brackish waters, as fish congregated at the river mouth. I suggest that movements into brackish waters are passive. Upstream movements began in late July and continued until freezing (November), with the intensity of movement varying annually according to climatic conditions. / Comparison between anadromous and freshwater trout showed very little difference in mean length at age, age at maturity, egg size and fecundity between the two groups. However, examination of energetic parameters indicated that sea run brook trout had a greater increase in condition factor during the summer than the freshwater residents. This raise in condition factor is accompanied by a decrease in water content and a lower water content in the eggs of anadromous fish. These results indicate that benefits associated with anadromous migrations in brook trout reside in the seasonal pattern of energy storage, which is important for the recovery from energy expenditure associated with spawning and overwintering.

No description available.

The relationships between brook trout (Salvelinus fontinalis), brown trout (Salmo trutta), non-game fish populations and selected physical and chemical parameters were investigated in four streams draining the Blue Ridge Mountains in north-central Virginia during low flow conditions in August, 1981 and 1982. Multiple regression and discriminant function analysis identified average stream width and maximum stream temperature as the main environmental factors related to brook and brown trout abundance and distribution. Furthermore, consistent fish species assemblages relative to brown trout distribution were observed in the study streams. The movements of tagged brown trout in two streams were assessed for the period from June 1981 to July 1982. Most brown trout remained within 200 m of original tagging sections, 98.7% in the Conway River (78 out of 79) and 71.4% in the North Fork Thornton River (7 out of 9). The 1979, 1978 and 1977 year class fish (ages 2+, 3+ and 4+) exhibited extremely restricted movements and were invariably recaptured at original capture locations while the 1980 year class (age 1+) displayed small net upstream movements (<80 m) throughout the period. Two 1980 year class individuals displayed extensive movements (17.0 km downstream and 1.2 km upstream). These movements were characteristic of other stream resident brown trout populations. Results of this study suggest that brown trout may expand their range upstream into currently allopatric brook trout zones. It would likely be a slow but steady process which could be accelerated by infrequent long-range movements. The potential for establishment of reproducing brown trout populations and impacts of brown trout on native brook trout populations can be assessed utilizing fish species assemblages, average stream width and maximum stream temperature prior to further brown trout introductions in this region of Virginia. / Master of Science

This study sampled salmonid populations in two headwater streams in East Tennessee, Briar Creek and Left Prong Hampton Creek. Length and weight data were used to calculate the growth of these populations to determine if significant variation exists between isolated brook trout populations. Slope comparisons concluded that there was a difference in growth between brook trout populations of these streams (p

This research supports development of aquatic resource management strategies to address acid deposition in the Great Smoky Mountain National Park (GRSM) by 1) developing relationships between baseflow and stormflow chemical constituents and examining effects of elevation, area, geology, soil, and vegetation on stream chemistry; 2) evaluating physiological condition in brook trout in relation to changes in stream chemistry during stream acidification episodes, and 3) evaluating brook trout metrics with respect to stream chemistry, basin characteristics, and ecologically relevant hydrologic parameters. (1) Stream chemistry was monitored in eight GRSM streams considering basin area, site elevation, Anakeesta geology, soil, and vegetation. Following precipitation events, pH was significantly reduced and aluminum concentrations increased, while the concentration response of ANC, nitrate, sulfate, and base cations varied. Higher pH and ANC concentrations were observed in large and low-elevation streams. (2) Caged brook trout were exposed to two acid episodes during in situ bioassays conducted in three GRSM streams. Stream pH decreased (>0.7 pH units) and total dissolved aluminum increased (>0.175 mg/L) at all three sites during acid episodes. Whole-body sodium concentrations were significantly reduced (10-20%) when preceding 24-h time weighted average pH values (4.88, 5.09, 4.87) and corresponding 24-h aluminum concentrations (210, 202, 202 µg/L). Lower whole-body sodium concentrations were correlated with elevated proton and aluminum concentrations indicating physiological distress. (3) Water chemistry, hydrology and physical basin factors influenced brook trout distributions and densities in 16 collocated fish and water quality sampling sites (1990-2009). Higher concentrations of ANC, pH, sodium, and soil cation exchange capacity, and higher fall flows were associated with the presence of brook trout. Trout densities were higher in streams with higher concentrations of sodium, suggesting that sodium may ameliorate the effects of acid toxicity. These relationships provide useful information where GRSM managers can prioritize conservation and restoration efforts.

Anthropogenic influences and climate change are warming rivers in New Brunswick and threatening the cold water habitats of native salmonids. When ambient river temperatures in summer exceed the tolerance level of Atlantic salmon and brook trout, individuals behaviourally thermoregulate by seeking out cold water refugia. These critical thermal habitats are often created by tributaries and concentrated groundwater discharge. Thermal infrared imagery was used to map cold water anomalies along a 53 km reach of the Cains River on 23 July 2008. Although efficient and useful for mapping surface temperature of a continuous stream reach, the fish did not use all identified thermal anomalies as refugia. Overall, 100 % of observed large brook trout >35 cm in length were found in 30 % of the TIR-mapped cold water anomalies. Ninety eight percent of observed small brook trout 8 – 30 cm in length were found in 80 % of the mapped cold water anomalies and their densities within anomalies were significantly higher than densities outside of anomalies. Fifty nine percent of observed salmon parr were found in 65 % of the mapped anomalies; however, they were dispersed within study sites and their densities were not significantly different within anomalies compared to outside of the anomalies. No brook trout were observed at the seven noncold water study sites that were investigated. Preference curves for various habitat variables including velocity, temperature, depth, substrate, and deep water availability near cold water anomalies were developed based on field investigations during high temperature events (ambient river temperature >21 oC). Combined with thermal imagery, managers can use the physical descriptions of thermal refugia developed here as a tool to help conserve and restore critical thermal refugia for Atlantic salmon and brook trout on the Cains River, and potentially similar river systems.

Thesis (M.S.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains x, 85 p. : ill. (some col.), maps (some col.). Includes abstract. Includes bibliographical references (p. 62-69).