Bull trout (Salvelinus confluentus) populations from Alberta river drainage systems were compared using molecular techniques. Restriction fragment length polymorphisms (RFLP's) within the NDI and ND5/6 regions of the mitochondrial genome were observed. In addition, randomly amplified polymorphic DNA profiles (RAPD's) from total genomic DNA extracts were compared. Interdrainage comparisons using mtDNA revealed significant population heterogeneity among Alberta bull trout. Percent sequence divergence in mtDNA ranged from 0.14% to 0.92%. Most fish in each population were composed of a small number of common haplotypes, and the remaining fish displayed rare or locally unique haplotypes. RAPD profiles were used to calculate genetic distance values for Alberta, Canada and Montana, U.S.A. populations. Both Nei and Cavalli-Sforza distance values were used to generate neighbor-joining, FITCH and KITSCH distance trees. Two genetically distinct groups of bull trout were revealed by the RAPD analysis and the possiblity that post-glacial bull trout populations are derived from two separate refugia is suggested. / xvii, 161 leaves : ill. ; 28 cm.

Bull trout are a species of fish native to the coldwater mountain streams of Alberta. Because this species is of special conservation concern and displays finely dissected population structure, it is well suited as a model species to test the utility of versatile conservation genetics tools. One such tool, a genetic clustering method, was used to discern the hierarchical population structure of bull trout in the core of their range in South-West Alberta. The method also revealed patterns of gene flow by way of assignment tests. Populations defined by this method were then used as reference populations for mixed-migrant assignment tests, revealing that clustering method-defined populations may be more suitable for such tests rather than traditional approaches that define reference populations by sampling location. Combined with spatial data a posteriori, assignment tests had additional utility of discerning spatial scale of movement for juvenile and adult salmonids. This technique provided further evidence that assignment tests may be powerful indirect tools for evaluating migration, and that longrange inter-stream dispersal in juvenile salmonid fish may be more common than previously assumed. / xi, 174 leaves : ill. ; 29 cm. --