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The Role of ERp57 in Hras Intracellular Trafficking and Function.Parman, Jaime Lyn 13 December 2003 (has links) (PDF)
Ras is a central player in signal transduction that mediates cellular proliferation and differentiation. Recent evidence has shown that lipid and non-lipid modified domains participate in Ras traffic and that plasma membrane association is mediated by vectorial vesicular transport from the endomembrane system. ERp57, an ER chaperone, has been shown to specifically bind farnesylated Hras but not non-farnesylated Hras. The objective of this study was to determine if ERp57 participates in Ras trafficking and function. First, the effect of ERp57 knock down by siRNA technology on Hras function was studied; there was a reduction in ERp57 cellular levels that led to a decrease of active ras. Second, specific anti-ERp57 antibodies were delivered into 3T3 cells expressing GFP-ras chimeras to observe the effect on intracellular trafficking. Anti-ERp57 antibodies blocked Hras plasma membrane localization but not Kras suggesting that ERp57 may be involved in Hras intracellular trafficking and function.
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<b>ELUCIDATION OF THE INITIAL SUBSTRATE ACCESS PATHWAY IN STE24, YEAST HOMOLOG OF ZMPSTE24</b>Eric Leonard Glasser (17605800) 11 December 2023 (has links)
<p dir="ltr">Premature aging disorders such as mandibuloacral dysplasia disorder (MAD) can be caused by improper maturation of nuclear scaffolding protein lamin A from its precursor prelamin A. ZMPSTE24 is responsible for both the earlier C-terminal CAAX cleavage and a subsequent N-terminal upstream cleavage during the posttranslational processing of prelamin A to lamin A. Although ZMPSTE24’s structure and function are well characterized, the role of the 4 apparent openings into its hollow inner chamber remains unknown. We hypothesize that one of these entrances, portal 1, is the initial substrate entry point based on its proximity to the zinc-coordinating active site. Unfortunately, ZMPSTE24 is difficult to express and purify. Fortunately, the yeast homolog, Ste24, not only shares many structural and functional similarities to ZMPSTE24 but is also much easier to express and purify in an active state. Therefore, we will use Ste24 and its substrate <b>a</b>-factor as a model system for ZMPSTE24 and its substrate prelamin A to deduce whether portal 1 acts as the primary substrate entry point. We examined portal 1’s function in primary substrate entry by observing how the incubation of portal 1 mutants engineered with cysteine residues around the portal with cysteine-reactive bismaleimide crosslinkers affects the activity of the C-terminal CAAX cleavage. If crosslinking of the cysteine residues occludes the portal, we hypothesize that activity will decrease because substrate cannot enter. The cysteine-less enzyme Ste24 (QA), which cannot react with the crosslinkers, was engineered by mutagenesis to contain 1 or 2 new cysteines at specific positions around this portal. We hypothesize that portal 1 occlusion with cysteine reactive bismaleimide crosslinkers will inactivate the enzyme by preventing substrate entry. We monitored changes in CAAX cleavage activity with a radioactive endoprotease-coupled CAAX assay.</p><p dir="ltr">In crude membranes derived from yeast expressing QA Ste24, activity was not inhibited in the presence of either BMH or BMOE crosslinker. For the single cysteine-containing mutants M210C, T267C, I307C, and V311C, each crosslinker similarly decreased activity over 50%. For the double cysteine-containing mutants M210C-I307C, T267C-I307C, and T267C-V311C, we found between 20-60% decreased activity in the presence of the crosslinker which has a length most similar to the distance between the two cysteines. These results closely reflect previous data and further suggest that CAAX activity of the enzyme may be decreased due to the occlusion of the primary entry site, portal 1.</p><p dir="ltr">With purified QA Ste24, changes in activity were less apparent. Activity for purified QA was not decreased in the presence of either crosslinker. Single cysteine-containing mutants did not show decreased activity in the presence of either crosslinker. Unlike what was observed in crude membrane preparations, the double-cysteine containing mutants exhibited minimal decrease in activity in the presence of the crosslinker that has a length most similar to the distance between the two cysteines.</p><p dir="ltr">In crude membrane preparations, the cysteine-containing QA Ste24 mutants have diminished activity in the presence of crosslinkers. This may be due to the occlusion of the primary entry point, portal 1. However, we recognize the possibility that the decrease in activity was the result of the occlusion of the exit portal site. It is imperative that further experiments confirm that exit portal occlusion is not occurring. For purified cysteine-containing QA Ste24 mutants, the negligible decrease in activity suggests either that the portal 1 is not the primary substrate entry point or that the conditions of the assay were not optimized to generate inhibition. For example, the concentration of crosslinker was not sufficient in the presence of excess lipid and the reconstitution mixture sequestered the crosslinker. Further optimization of the reaction conditions is warranted. The cysteine-containing QA Ste24 mutants must be assessed for free thiols to determine how successful the reaction with the crosslinkers is. A more developed understanding of how all four portals function in the Ste24 CAAX processing of <b>a</b>-factor will be very insightful towards the mechanism of ZMPSTE24 in lamin A CAAX processing and may catalyze new targets of study for ZMPSTE24-related diseases like MAD-B.</p>
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<b>ANALYSIS OF THE SUBSTRATE SPECIFICITY AND BINDING SITE OF THE YEAST ZINC METALLOPROTEASE, STE24</b>Shanica Mariah Brown (18429576) 24 April 2024 (has links)
<p dir="ltr">The yeast zinc metalloprotease, Ste24, is involved in the maturation of the yeast mating pheromone <b>a</b>-factor by performing two distinct cleavages in the same precursor peptide substrate. Firstly, during the CaaX processing, Ste24 cleaves the three terminal residues of <b>a</b>-factor. CaaX processing is a well-studied process that involves the prenylation, proteolysis, and carboxyl-methylation of proteins ending with a cysteine (C), two aliphatic residues (aa), and one of several amino acids (X). The second cleavage step by Ste24 occurs after CaaX processing and involves an upstream cleavage N-terminal to the CaaX site. Another cleavage is performed by the enzyme Axl1 before the precursor peptide is transported from the cell to initiate mating processes. Inhibition of Ste24 typically results in ‘sterile’ cells which is how the term ‘Sterile 24’ was coined. In humans, defects in this metalloprotease or its substrate, Prelamin A, typically result in a range of progeroid disorders. Furthermore, the severity of these diseases has been directly linked to the catalytical activity of the enzyme. Treatments for these diseases are difficult to develop due to the limited knowledge available on the catalysis, substrate recognition, and functions of Ste24 and its homolog.</p><p dir="ltr">As such, these studies aim to define the substrate specificity of Ste24 and elucidate the binding site of Ste24. Identifying the substrate requirements of Ste24 has been an increasingly interesting topic due to the implication of Ste24 in a variety of unrelated functions. Previously, it has only been shown that yeast Ste24 is able to cleave the native substrate, the precursor of <b>a</b>-factor, and the substrate of its human homolog, prelamin A. This is an interesting finding because both substrates have dissimilar sequences at each cleavage site; so, it could be hypothesized that Ste24 may be able to recognize a wider range of sequences than expected. Further research has provided evidence that Ste24 is able to cleave both prenylated and non-prenylated substrates. It is also able to act as a translocon unclogger which may support its function in cleaving toxic islet amyloid polypeptides involved in cell failure in diabetes. Surprisingly, it was shown that this ‘unclogger ability’ was directly correlated to the activity level of Ste24, suggesting that the active site is directly involved in cleaving these peptides. With this information, it is clear that Ste24 has a broader substrate recognition ability than previously believed.</p><p dir="ltr">To elucidate the substrate specificity of Ste24, short peptide sequences containing varying CaaX sequences were developed and tested for C-terminal activity through a radioactive methyltransferase-coupled diffusion assay. Ste24 was able to recognize several sequences, however, a larger library is necessary to identify the specific requirements necessary for cleavage. Secondly, we tested the necessity of carboxylmethylation for the upstream N-terminal cleavage. The Distefano group designed three 33-mer analogs of <b>a</b>-factor, developed to mimic the C-terminally cleaved peptide. These peptides had either <b>a)</b> a methyl ester terminus representing the native substrate, <b>b)</b> a free carboxyl terminus representing the unmethylated precursor, and <b>c)</b> an amide terminus representing an unnatural end. All three peptides were tested using a FRET-based assay that allowed for the kinetic parameters of each peptide to be evaluated. We demonstrated that carboxylmethylation was not necessary for the upstream N-terminal cleavage; all three peptides presented similar kinetics. Finally, we interrogated the binding site of Ste24 through the use of a radioactive methyltransferase-coupled diffusion assay (C-terminal cleavage), a FRET-based assay (N-terminal cleavage), and photocrosslinking assays (binding). Together, these data presented a clearer image of residues necessary for the cleavage and binding of substrates within Ste24.</p>
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