AN ABSTRACT OF THE THESIS OF MARCUS C. ABSTON, for the Master of Science degree in ZOOLOGY, presented on 18 April 2017, at Southern Illinois University Carbondale. TITLE: EFFECTS OF OLFACTORY ENRICHMENT ON AFRICAN CHEETAHS (ACINONYX JUBATUS) MAJOR PROFESSOR: Dr. Carey Krajewski Environmental enrichment has been an essential part of felid husbandry in zoos, serving to reduce both physiological and psychological stress. Olfactory enrichment is one of many interventions used to prevent stereotypic behavior caused by stress in felids. However, little research has been done on this practice. The purpose of this study was to compare behavioral responses of four captive African cheetahs to six types of commercial fragrances used as olfactory enrichment stimuli. Three of these fragrances are marketed as “men’s cologne” and three as “women’s perfume”. The fragrances were also categorized as musky, spice, and floral types. Behavioral responses were recorded by live observations during 36 bouts at the St. Louis Zoo. There was a significant difference between cheetahs’ interaction time with male and female fragrances; they seemed to prefer male colognes. However, there was no significant difference in interaction time among fragrance types. Engagement behaviors varied among individuals (e.g. sniffing, scent rolling, pawing), but were similar to those reported by previous authors. These findings suggest that commercial fragrances may be a useful option for African cheetah olfactory enrichment.

We propose subword spotting, a generalization of word spotting where the search is for groups of characters within words. We present a method for performing subword spotting based on state-of-the-art word spotting techniques and evaluate its performance at three granularitires (unigrams, bigrams and trigrams) on two datasets. We demonstrate three applications of subword spotting, though others may exist. The first is assisting human transcribers identify unrecognized characters by locating them in other words. The second is searching for suffixes directly in word images (suffix spotting). And the third is computer assisted transcription (semi-automated transcription). We investigate several variations of computer assisted transcription using subword spotting, but none achieve transcription speeds above manual transcription. We investigate the causes.

We propose subword spotting, a generalization of word spotting where the search is for groups of characters within words. We present a method for performing subword spotting based on state-of-the-art word spotting techniques and evaluate its performance at three granularitires (unigrams, bigrams and trigrams) on two datasets.We demonstrate three applications of subword spotting, though others may exist. The first is assisting human transcribers identify unrecognized characters by locating them in other words. The second is searching for suffixes directly in word images (suffix spotting). And the third is computer assisted transcription (semi-automated transcription). We investigate several variations of computer assisted transcription using subword spotting, but none achieve transcription speeds above manual transcription. We investigate the causes.

Short track speed skating has become popular all over the world. The demands of a computer-aided training (CAT) system are booming due to this fact. However, the existing commercial systems for sports are highly dependent on expensive equipment and complicated hardware calibration. This dissertation presents a novel CAT system for tracking multiple skaters in short track skating competitions. Aiming at the challenges, we utilize global rink information to compensate camera motion and obtain the global spatial information of skaters; apply Random Forest to fuse multiple cues and predict the blobs for each of the skaters; and finally develop a silhouette and edge-based template matching and blob growing method to allocate each blob to corresponding skaters. The proposed multiple skaters tracking algorithm organically integrates multi-cue fusion, dynamic appearance modeling, machine learning, etc. to form an efficient and robust CAT system. The effectiveness and robustness of the proposed method are presented through experiments.

The Gram-negative bacterium Bartonella henselae (Bh) is an emerging zoonotic pathogen that has been associated with a variety of human diseases including bacillary angiomatosis which is characterized by vasoproliferative tumor-like lesions on the skin and internal organs of some immunosuppressed individuals. Several virulence factors associated with Bartonella-induced pathogenesis have been characterized. However, the study of those virulence factors has been limited to in vitro cell culture systems due to the lack of a practical animal model. Therefore, we wanted to investigate whether the zebrafish embryo (Danio rerio) could be used to model human infection with Bh. We investigated if Bh can mount an infection in zebrafish embryos during their early stage of development. Our data showed that Tg(fli1:egfp)y1 zebrafish embryos supported a sustained Bh infection for 7 days with >10-fold bacterial replication when inoculated in the yolk sac. This was evident by plating of zebrafish homogenates, quantitative PCR, and confocal microscopy analysis. We assessed the interaction of Bh with EC and the phagocytic cells in live embryos by microscopy. Our data showed that aggregates of Bh interact with the endothelium of the embryo vasculature. Evidence showed that Bh recruited phagocytes to the site of infection in the Tg(mpx:GFP)uwm1 embryos. We also wanted to determine the response to infection with Bh. Infected embryos showed evidence of a Bh-induced angiogenic phenotype as well as an increase in expression of genes encoding pro-inflammatory factors and pro-angiogenic markers. A deletion mutant for the entire VirB type IV secretion system (ΔvirB2-11 supported bacterial replication although to a lesser degree compared to the wild type control. However, infection of zebrafish embryos with a deletion mutant in the major adhesin (BadA) resulted in little or no bacterial replication and a diminished pro-angiogenic and pro-inflammatory host response compared to wild type Bh, providing the first evidence that BadA is critical for in vivo infection. Thus, the zebrafish embryo provides the first practical animal model of Bh infection that will facilitate efforts to identify virulence factors and define molecular mechanisms of Bh pathogenesis.

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Die immunologische Kontrazeption mittels Zona pellucida (ZP) Proteinen gilt als vielversprechender Ansatz für die Reproduktionskontrolle verwilderter Haus- und Wildtierbestände. Da die Applikation von nativer ZP mit Nebenwirkungen verbunden ist, wird die Verwendung einzelner ZP Peptide als Bestandteil kontrazeptiver Vakzine als besonders aussichtsreich erachtet. Das Prinzip dieser nebenwirkungsfreien ZP Immunisierung ist die gezielte Trennung der Entzündungsreaktionen auslösenden T-Zell-Epitope der ZP von den kontrazeptiv wirkenden B-Zell-Epitopen.<br> Niedermolekulare synthetische oder rekombinante Peptide allein sind gering immunogen und können somit keine ausreichende Immunantwort induzieren. Die Verwendung von Peptiden für die immunologische Kontrazeption erfordert daher ein &bdquo;Vakzin-Design&ldquo;, d. h. die gezielte Kombination der Peptide mit immunstimulierenden Substanzen (Liposomen, Carrierproteinen, Adjuvantien). Zielstellung der vorliegenden Arbeit war die Untersuchung des Potentials synthetischer Peptide für die Immunokontrazeption von verwilderten Hauskatzen (Felis catus). Dazu wurden zunächst relevante B-Zell-Epitope des felinen Zona pellucida Proteins, ZPB2, identifiziert und synthetisiert. Zwei der synthetischen Peptide (P3, P6) wurden zur Herstellung von Antikörpern an BSA konjugiert und zusammen mit Freundschem Adjuvans in Ratten verimpft. Die kontrazeptive Relevanz beider Peptide sowie der Ratten Anti-Peptid Antiseren wurde im in vitro Befruchtungssystem der Hauskatze geprüft. Zur Untersuchung der Immunogenität der Peptide in der Zielspezies Hauskatze erfolgte die Entwicklung von Vakzin-Prototypen für die einmalige Applikation. Neben der Eruierung der Stärke und Dauer der Immunantwort wurde durch Verpaarung der Tiere auch das kontrazeptive Potential in vivo abgeschätzt. / Immunological contraception based on zona pellucida (ZP) proteins is regarded as a promising approach for the control of reproduction in feral domestic and wild animals. Because application of native ZP caused adverse reactions, utilization of single ZP peptides as elements of contraceptive vaccines were considered to bear good prospects. The principle of this ZP immunization is based upon a systematic separation of inflammation triggering T-cell epitopes from the contraceptive B-cell epitopes.<br> The present study evaluates the immunogenicity and contraceptive potential of synthetic feline ZPB2 peptides for immunocontraception in cats (Felis catus). First of all, relevant B-cell epitopes were identified and synthesized. In order to generate antipeptide antibodies two peptides (P3, P6) were chosen and coupled to BSA. Rats were immunized with the conjugation product combined with Freund&prime;s complete adjuvant.<br> The contraceptive efficacy of both peptides and of the anti-peptide antibodies generated were determined using in vitro fertilization of feline oocytes (IVF). To evaluate the peptides immunogenicity in the target species (cat), vaccine-prototypes were developed for a single application protocol. The strength and duration of the immune response was analyzed. Additionally, cats were mated to assess the contraceptive potential of the vaccines in vivo.

Reinsurance is one of the most important tools to be used by insurance companies, for managing risks. This is an effective way / however, there are situations where reinsurance is insufficient, such as the occurrence of a natural hazard. When a natural hazard occurs, many insured experience loss at the same time, which drains the reinsurance market capacity. If future market capacity could be forecasted, then it would be easier for companies to decide when to include cat bonds or any other additional securities in their portfolio. In order to establish a model for market capacity, its relationship with other market parameters and the association among parameters are examined. In this study, these relationships are analyzed and used to establish an algorithm for predicting the next years reinsurance capacity. Moreover, last 10-year data for market capacity is used to establish and AR(1) model, in order to create a comparison with the algorithm. A case study of cat bonds is done, which uses the pricing load calculation of the Lane model and aims to ease the decision-making process by comparing the loads of cat bond and reinsurance pricing.

This thesis consists of an introduction and four papers. All four papers are devoted to problems in Number Theory. In Paper I, a special class of local ζ-functions is studied. The main theorem states that the functions have all zeros on the line Re(s)=1/2.This is a natural generalization of the result of Bump and Ng stating that the zeros of the Mellin transform of Hermite functions have Re(s)=1/2.In Paper II and Paper III we study eigenfunctions of desymmetrized quantized cat maps.If N denotes the inverse of Planck's constant, we show that the behavior of the eigenfunctions is very dependent on the arithmetic properties of N. If N is a square, then there are normalized eigenfunctions with supremum norm equal to <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?N%5E%7B1/4%7D" />, but if N is a prime, the supremum norm of all eigenfunctions is uniformly bounded. We prove the sharp estimate <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?%5C%7C%5Cpsi%5C%7C_%5Cinfty=O(N%5E%7B1/4%7D)" /> for all normalized eigenfunctions and all $N$ outside of a small exceptional set. For normalized eigenfunctions of the cat map (not necessarily desymmetrized), we also prove an entropy estimate and show that our functions satisfy equality in this estimate.We call a special class of eigenfunctions newforms and for most of these we are able to calculate their supremum norm explicitly.For a given <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?N=p%5Ek" />, with k&gt;1, the newforms can be divided in two parts (leaving out a small number of them in some cases), the first half all have supremum norm about <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?2/%5Csqrt%7B1%5Cpm%201/p%7D" /> and the supremum norm of the newforms in the second half have at most three different values, all of the order <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?N%5E%7B1/6%7D" />. The only dependence of A is that the normalization factor is different if A has eigenvectors modulo p or not. We also calculate the joint value distribution of the absolute value of n different newforms.In Paper IV we prove a generalization of Mertens' theorem to Beurling primes, namely that \lim_{n \to \infty}\frac{1}{\ln n}\prod_{p \leq n} \left(1-p^{-1}\right)^{-1}=Ae^{\gamma}<img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?%5Clim_%7Bn%20%5Cto%20%5Cinfty%7D%5Cfrac%7B1%7D%7B%5Cln%20n%7D%5Cprod_%7Bp%20%5Cleq%20n%7D%0A%5Cleft(1-p%5E%7B-1%7D%5Cright)%5E%7B-1%7D=Ae%5E%7B%5Cgamma%7D," />where γ is Euler's constant and Ax is the asymptotic number of generalized integers less than x. Thus the limit <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?M=%5Clim_%7Bn%5Cto%5Cinfty%7D%5Cleft(%5Csum_%7Bp%5Cle%20n%7Dp%5E%7B-1%7D-%5Cln(%5Cln%20n)%5Cright)" />exists. We also show that this limit coincides with <img src="http://www.diva-portal.org/cgi-bin/mimetex.cgi?%5Clim_%7B%5Calpha%5Cto%200%5E+%7D%0A%5Cleft(%5Csum_p%20p%5E%7B-1%7D(%5Cln%20p)%5E%7B-%5Calpha%7D-1/%5Calpha%5Cright)" /> ; for ordinary primes this claim is called Meissel's theorem. Finally we will discuss a problem posed by Beurling, namely how small |N(x)-[x] | can be made for a Beurling prime number system Q≠P, where P is the rational primes. We prove that for each c&gt;0 there exists a Q such that |N(x)-[x] | / QC 20100902

The subject of the thesis is a study of the projections from low threshold oral and face afferents to the cerebral cortex and of descending motor control mechanisms originating in the cerebral cortex or the hypothalamus and influencing the jaw reflexes.Cats anaesthetized with chi oral ose were used for the experiments. Ipsi- and contralateral nerves from the oral cavity and the face were stimulated electrically. Cortical potentials were averaged and recorded. The location of the projections was related to the cytoarchi-tectonic areas of the cerebral cortex. It was found that the afferents projected to separate maximum points in areas 3a, 3b, 5a and 6aß. The projections to areas 3a and 3b were somatotopically organized, but the layout of the projections on the cortex was not facelike.The effect of monopolar anodal stimulation of the cerebral cortex on the monosynaptic jaw closing and the di synaptic jaw opening reflexes was investigated. A sequence of facilitation and inhibition of both reflexes was elicited by cortical stimulation. The effects were of short latency (2.5 ms) and could start with either facilitation or inhibition. The timecourse of the sequence was sinuslike with a period of 10 ms. The largest effect originated in the "sensory" areas 3a and 3b and not in the "motor" areas 4y and 6ag. It is suggested, that a tri gemino-cortico-tri geminai loop via area 3a may function in reflex modulation of jaw movements.The hypothalamic effects on the jaw reflexes were evoked by electrical stimulation in those parts of the hypothalamus, which are w known to generate defence, attack or feeding responses. A tenfold facilitation of the jaw closing reflex and a facilitation followed by almost complete inhibition of the jaw opening reflex were observed in the anaesthetized animal with intact cerebral cortex. The effects remained but were diminished in amplitude after cortical ablation. The descending path was located in the ventral midbrain tegmentum.It is suggested that the observed hypothalamo-tri geminai mechanism may exercise a tonic influence on the trigeminal motoneurones, thereby controlling the set points of the biting force and the rest position. The implications of this hypothesis on the etiology of bruxism and the myofascial pain-dysfunction are discussed. / <p>Härtill 3 rapporter.</p> / digitalisering@umu