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The genetic analysis of the vulval cell lineages of Caenorhabditis elegans /Ferguson, Edwin L. January 1900 (has links)
Thesis (Ph. D.)--Massachusetts Institute of Technology and Woods Hole Oceanographic Institution. 1985. / Bibliography: p. 325-332.
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Modulation of breast cancer tumour-initiating cells in cell lines and patient-derived tumour xenograftsSandoval, José Luis Bico Rosa Gamero January 2015 (has links)
No description available.
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Classification of Breast Cancer Cell Lines into Subtypes Based on Genetic ProfilesPawar, Aniruddha Vikram 16 March 2015 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Today we know that there are several different types of breast cancer. Accurate identification breast cancer subtype is extremely important in treating this disease effectively. Consequently the process of invtro development of drugs to treat this disease should be naturally subtype specific. Until now several studies have identified multiple breast cancer cell lines and these cell lines have served as invaluable invitro tumor models. However very few of these cell lines are classified as per their subtypes. In this thesis an effort is made to classify 59 of such breast cancer cell lines using genetic profile comparison approach. This approach is based on comparing characteristic features such as copy number and gene expression of a given cell line to those observed from the tissue samples of different breast subtypes. The tissue data for this comparison comes from The Cancer Genome Atlas (TCGA) while cell line data is taken from Cancer Cell Line Encyclopedia (CCLE).
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Expression of genes and differentiation markers in human glioblastoma cell linesGillaspy, Glenda E. January 1991 (has links)
No description available.
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Dopaminergic regulation of gonadotropin-releasing hormone (GnRH) secretion and gene expression in a GnRH neuronal cell line曾美好, Tsang, May-ho. January 1995 (has links)
published_or_final_version / Zoology / Master / Master of Philosophy
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The regulation and characterization of porcine IgECorfield, Gaynor Christa January 1995 (has links)
No description available.
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Characterisation of a novel multi-tissue tumour suppressor gene in mouseO'Neill, Vincent John January 2001 (has links)
No description available.
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Cellular mechanisms of sensitivity and resistance to platinum agentsO'Neill, Ciaran Francis January 1999 (has links)
No description available.
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The genetics of miRNA and mRNA expression in human lymphoblastoid cell linesWills, Quintin Frank January 2012 (has links)
Human clinical genome-wide association studies (GWAS) have helped identify disease trait and pharmacogenomic loci without the need for biological under- standing. Molecular GWAS - associating genetic variation with traits such as gene expression - have been slow to fill the mechanistic gaps. While tissue specificity, lack of DNA resolution, and the need for better data integration are no dou bt important bottlenecks in molecular GWAS, there is also a very poor general understanding of which molecular phenotypes are important and how best to model them. Added to this is the clear need for a greater understanding of the strengths and weaknesses facing in vitro (and ex vivo) models as hypoth- esis generating and GWAS validation tools. The studies in this work focus on RNA expression in a popular human model: lymphoblastoid cell lines (LCLs). Chapters 2 and 3 examine microRNA (miRNA) and messenger RNA (mRNA) expression in a total of 300 genotyped human LCLs. The expression of only one miRNA could be associated with a nearby genetic variant. This result was observed in both the African and European samples studied, in a separate val- idation data set, and was technically validated with quantitative PCR. While limited genotype resolution and small sample sizes are likely to be important contributors to this low hit rate, the results strongly suggest experimental con- founders. Highly expressed miRNAs reflected the transformed nature of the cells, highly correlated miRNAs enriched for EBV and malaria associated tar- get mRNA genes, and several miRNAs that were differentially expressed be- tween the European and African samples suggested differential EBV transfer- mation. Chapter 4 presents a study on single cells from some of the same samples, to test the hypothesis that the lack of tissue spatial resolution is an important limiting factor in human genetic epidemiology. Experimental con- founders were also considered: sample growth was found to associate with the expression of several genes. Cell-to-cell gene correlations and distributions made it possible to propose how genes change their expression, functionally differ from each other, and are able to alter their behaviours without altered whole-tissue expression. The results suggest which type of genes are more likely to be susceptible to genetic effects, and propose promoter behaviours altered by genetic variants located near to 13 genes. From these whole-tissue and single cell results the broad conclusion is that, while LCLs are likely to be inappropriate for the study of miRNA genetics, their functional genomics at higher spatial resolution shows promise as a more mechanistic approach for the study of germline genetics.
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Expression patterns of immune associated genes in Euoniticellus intermedius and characterization of the embryonic cell lineAlaouna, Mohamed 01 February 2013 (has links)
As bacteria are becoming resistant to conventional antibiotics, researchers are looking for
new ways to combat microbial infection. We have begun to adopt genetic and functional genomic
approaches to define the molecular determinants of pathogen resistance in the dung beetle,
Euoniticellus intermedius. This dung beetle survives microbe-rich environments such as dung. This
ability makes it a potential model for the study of infectious agents and ecological damage. To date,
E. intermedius has not been studied at the molecular level. In this study, a range of complimentary
analytical techniques were used to characterize the E. intermedius embryonic cell line established in
our laboratory. These techniques characterize morphology, growth characteristics, karyotype,
isoenzyme patterns and embryonic development. Complete characterization of the E. intermedius
cell line is essential for the cell banks and for the regulatory requirements in biopharmaceutical
production.
This study followed gene sequences and their comparisons for both adult and cell line to
confirm that the E. intermedius (EISA08) cell line is originated from the embryonic E. intermedius
dung beetle. cDNA was synthesized from mRNA isolated from E. intermedius adult beetles and cell
line (EISA08) was sequenced using GS (FLX) technology by a commercial facility, Inqaba
Biotechnical Industries (Pty) Ltd, South Africa. In addition to characterization of the cell line, two
genes, namely hopscotch and ribosomal protein S9 (RpS9), were selected from the Flylab genome
data base. The E. intermedius database is a web-based system for the genome and transcriptome of
the dung beetle to evaluate the immune system of the dung beetle (http://Flylab.wits.ac.za/).
hopscotch was selected because it is believed to be involved in the JAK-STAT signalling pathway
for anti-viral response, embryonic development and cell growth. Rsp9 was chosen as a loading
control because it is expected to be a housekeeping gene. The conserved molecular signalling pathway JAK-STAT is used by E. intermedius (as in other insects and humans) for immune defence
and early embryonic development. The project followed hopscotch and Rsp9 gene expression in all
the E. intermedius life cycle developing stages; adult, pupae, larvae, embryo, and cell line cell
growth, life cycle developing stages and embryonic development has was monitored.
E. intermedius embryonic development is described as short germ-band. E. intermedius
embryogenesis is regarded as basal and is observed in most arthropods. The study revealed that
E. intermedius hopscotch is over expressed in the early developing stages, embryo, larvae, and
pupae and in the newly established cell line EISA08. The results from this study lead to the
suggestion that E. intermedius JAK-STAT pathway is activated early and has an important role in
embryonic development, cell proliferation and immune defence. Studies of E. intermedius could
provide more insight into the properties and evolution of innate immunity and embryonic
development.
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