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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

Evaluation of a human colon adeno-carcinoma (Caco-2) cell line for isolation of respiratory viruses in nasopharyngeal aspirates frompatients with respiratory disease

Yan, Mei-kum., 甄美琴. January 2012 (has links)
Background: Isolation of respiratory viruses routinely requires a panel of cell lines. Management of these cell lines is usually considered complex, cumbersome, long turnaround time and high cost. In order to improve the detection rate and cost, there is a need to evaluate any other cell line that could be as sensitive as the routine cell line for the detection of respiratory viruses. The human colon adeno-carcinoma (Caco-2) cell line has been shown to support the growth of enteroviruses, enteric viruses, and influenza A virus, and ability to grow coronavirus NL63 and SARS from culture isolates. In the present study, the isolation rate of respiratory viruses in Caco-2 from clinical specimens was studied and compared with the conventional panel of cell lines for the diagnosis of respiratory virus disease. Material and methods: The study was performed in two stages. In the first stage, one hundred and eighty-nine nasopharyngeal aspirates confirmed positive by direct immunofluorescence were used to inoculate onto Caco-2, and HEp-2, A549, MDCK, LLC-MK2 cell lines at the same time. In the second stage, fifty-six nasopharyngeal aspirates were randomly selected and cultured on Caco-2, HEp-2, A549, MDCK and LLC-MK2. Infected cells were examined daily for cytopathic effect for up to 10 days. Virus was further identified by performing direct immunofluorescence test for detection of eight common respiratory viruses (respiratory syncytial virus, influenza A and B viruses, parainfluenza type 1, 2, 3, 4 viruses and adenovirus). Results: Caco-2 (84%) was found to be the most efficient cell line to propagate the respiratory viruses from nasopharyngeal aspirate when compared with any positive by these conventional panel cells (78%) or individual cell line MDCK (38%), HEp-2 (21%), LLC-MK2 (27%) and A549 (37%). The latter differences were statistically significant (p = <0.000001). Furthermore, Caco-2 recovered 86% (36/42) viruses of conventional panel cells negative samples, while conventional panel cells recovered 80% (24/30) viruses of Caco-2 cells negative samples. Conclusion: Caco-2 is sensitive to a wide range of virus and can greatly simplify routine cell culture service for isolation of respiratory viruses. / published_or_final_version / Microbiology / Master / Master of Medical Sciences

Targeted retroviral infection in the study of hematopoietic cell development

Murphy, George J. January 2002 (has links)
No description available.

Chromosomal translocation at chromosome 12q24.1 in high-grade non-Hodgkin's lymphoma : defining a new gene family

Zani, Valter January 2000 (has links)
No description available.

Development, characterisation and usage of hybrid pancreatic B-cell lines

Yoon, T. W. January 1992 (has links)
No description available.

Characterisation of the endoplasmic reticulum stress proteins GRP78 and GRP94 and their interaction with a recombinant antibody

Pearce, Alison January 2000 (has links)
No description available.

Anti-tumour 2-(4-aminophenyl)benzothiazoles : structurally simple but exciting candidates for clinical trials

Chua, Mei-Sze January 1999 (has links)
No description available.

Modulation of breast cancer tumour-initiating cells in cell lines and patient-derived tumour xenografts

Sandoval, José Luis Bico Rosa Gamero January 2015 (has links)
No description available.

Exploration of Classic Confounders in Lymphoblastoid Cell Lines used to Study Select Antineoplastic Agents

Doetsch, Natalie, Harder-Ibarola, Kimberly, Sheth, Aliyah January 2010 (has links)
Class of 2012 Abstract / OBJECTIVES: Therapeutic response to chemotherapeutic agents in vitro can be studied using immortalized lymphoblastoid cell lines (LCLs). While LCLs provide a valuable model to study heritable factors and anticancer drug reponse in large populations, the results may be confounded by properties inherent to the model. This study is used to explore possible confounders in Choy et al.’s publicially available dataset (Accession#: GSE11582). METHODS: This study utilized Affymetrics U133A array gene expression and phenotypic data for 162 unrelated LCLs. SPSS was used for two-tailed bivariate Pearson correlation analysis comparing relative 6-mercaptopurine (6-MP), 5-fluorouracil (5-FU), and methotrexate sensitivities, growth rate and ATP levels. GeneSpring was used to compare the top and bottom quartiles of relative ATP levels using the unpaired T-test with a significance threshold of 0.001 and Benjamin-Hochberg FDR (n=82). RESULTS: It was found that relative sensitivities of 5-FU and 6-MP are significantly correlated (r2= 0.627, p<0.0001). Furthermore, it was determined that 5-FU sensitivity and growth rate and ATP levels are also correlated; however, no significant correlation was found between growth rate and ATP levles (r2=0.127, p=0.107). Relative ATP level was found to be a more significant determinant of 5-FU sensitivity than growth rate. GeneSpring analysis showed that 1500 genes are differentially regulated based on ATP levels. The gene ontology related to nucleic acid metabolism was overrepresented (p=1.425E-15). CONCLUSIONS: The results above suggest that growth rate and, to a greater extent, baseline ATP levels influence genetic expression of LCLs and may confound in vitro studies of antineoplastic agents.

Classification of Breast Cancer Cell Lines into Subtypes Based on Genetic Profiles

Pawar, Aniruddha Vikram 16 March 2015 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Today we know that there are several different types of breast cancer. Accurate identification breast cancer subtype is extremely important in treating this disease effectively. Consequently the process of invtro development of drugs to treat this disease should be naturally subtype specific. Until now several studies have identified multiple breast cancer cell lines and these cell lines have served as invaluable invitro tumor models. However very few of these cell lines are classified as per their subtypes. In this thesis an effort is made to classify 59 of such breast cancer cell lines using genetic profile comparison approach. This approach is based on comparing characteristic features such as copy number and gene expression of a given cell line to those observed from the tissue samples of different breast subtypes. The tissue data for this comparison comes from The Cancer Genome Atlas (TCGA) while cell line data is taken from Cancer Cell Line Encyclopedia (CCLE).

Expression of genes and differentiation markers in human glioblastoma cell lines

Gillaspy, Glenda E. January 1991 (has links)
No description available.

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