Global ETD Search

1	Extraction of Proliferation and Death Rates in Cytokine-stimulated Erythroid Progenitors Using Cell-division Tracking and Mathematical Modeling Vahe, Akbarian 11 August 2011 (has links) Controlling fates of stem and progenitor cells is one of the central goals of regenerative medicine. However, conventional cell enumeration methods are unable to distinguish between the effects of cell death, proliferation, and differentiation through molecular interventions on the output of specific cell types. We have devised a strategy to simultaneously obtain proliferation and death rates in cultures of highly purified erythroid progenitors. The approach is based on combining cell-surface marker analysis, cell-division tracking and 7-amino-actinomycin-D staining to monitor cell death. A compartment model of cell proliferation was developed to evaluate cell generation-specific length of cell-division, rates of entry into division, and cell death, from the experimental cell-division tracking data obtained following stimulation with erythropoietin (EPO) and Stem cell factor (SCF). The results indicated that EPO and SCF, either as single factor or in combination, differentially affect the rates of differentiation, length of cell-division and rates of death. erythroid mathematical modeling stem cells cell division differentiation CFSE CFDA-SE red blood cell 0541
2	Extraction of Proliferation and Death Rates in Cytokine-stimulated Erythroid Progenitors Using Cell-division Tracking and Mathematical Modeling Vahe, Akbarian 11 August 2011 (has links) Controlling fates of stem and progenitor cells is one of the central goals of regenerative medicine. However, conventional cell enumeration methods are unable to distinguish between the effects of cell death, proliferation, and differentiation through molecular interventions on the output of specific cell types. We have devised a strategy to simultaneously obtain proliferation and death rates in cultures of highly purified erythroid progenitors. The approach is based on combining cell-surface marker analysis, cell-division tracking and 7-amino-actinomycin-D staining to monitor cell death. A compartment model of cell proliferation was developed to evaluate cell generation-specific length of cell-division, rates of entry into division, and cell death, from the experimental cell-division tracking data obtained following stimulation with erythropoietin (EPO) and Stem cell factor (SCF). The results indicated that EPO and SCF, either as single factor or in combination, differentially affect the rates of differentiation, length of cell-division and rates of death. erythroid mathematical modeling stem cells cell division differentiation CFSE CFDA-SE red blood cell 0541
3	Identifizierung genetischer Biomarker für die Wirksamkeit von Oxaliplatin:Kandidatengen-bezogene und Genom-weite Analysen / Identification of genetic biomarkers for the efficacy of oxaliplatin - candidate gene and genome-wide approaches Saman, Sadik 02 December 2014 (has links) No description available. 610 Oxaliplatin Kolorektales Karzinom Cisplatin Carboplatin rs11793978 rs74382821 micro-RNA hsa-miR-1277-3p individualisierte Therpaie Genom-weite Analyse ERCC1 ERCC2 ERCC5 Zytotoxizität CFSE Vybrant Ruby Sytox Zellproliferation Zellvitalität Proliferationshemmung DACH-Ligand SLC31A1 LCL lymphoblastoide Zelllinien 1000 human genomes HapMap Intron 1 SLC31A1 lymphoblastoid cell lines ERCC1 SLC31A1 rs74382821 rs11793978 genome-wide HapMap 1000 human genomes cisplatin carboplatin micro-RNA hsa-miR-1277-3p colorectal carcinoma ATP7A ATP7B Oxaliplatin cytotoxocity cell vitality cell proliferation Sytox Vybrant Ruby CFSE counting beads LCL Intron 1 SLC31A1 Medizin (PPN619874732)

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