Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-08-03T22:07:54Z No. of bitstreams: 1 DISSERTA??O - RENATA VIEIRA SILVA.pdf: 1258695 bytes, checksum: 8741b07320d6f4c024d94e4aa2fd913a (MD5) / Made available in DSpace on 2015-08-03T22:07:54Z (GMT). No. of bitstreams: 1 DISSERTA??O - RENATA VIEIRA SILVA.pdf: 1258695 bytes, checksum: 8741b07320d6f4c024d94e4aa2fd913a (MD5) Previous issue date: 2015-03-19 / Fruits of Spondias purpurea L. (Anacardeacea), popularly known as seriguela,are rich with phenolic compounds, which can be applied in the production of phytocosmetic. This study aimed to analyze the chemical constituents of the metanolic crude extract of S. purpurea (EBMSP) by high performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-MSn) and to evaluate this extract as principle active in photoprotective formulations. In addition, the flavonoid and phenolic content, sequestration activity of free radicals by DPPH method and antimicrobial activity by the inhibition zone method, were investigated. HPLC-ESI-MSnanalyses were possible to characterize five phenolic compounds, including phenolic acids, tannins and flavonols. The extract showed phenolic content and flavonoid values at 28.68 ? 0.046 mg gallic acid equivalent/g and at 2.64 ? 0.005 mg of flavonoid quercetin equivalent/g of extract, respectively. In vitro antioxidant activity showed percentage of inhibition of 74.41 to 250 ?g/mL, a high value compared with the rutin. The EBMSP demonstrated sunscreen activity, with solar protection factor (SPF) values> 6.0 at low dilutions and UVA protection equal to the reference standards benzophenone-3 and rutin. The best results among formulations 5, 10, 20 and 30% for UVA and UVB sunscreens profile corresponded to lotion 30%, however, no formulations showed synergism with the standard benzophenone-3. To evaluate the antimicrobial activity, suggests that the EBMSP has activity against Gram-negative micro-organisms Pseudomonas aeruginosa and Salmonella dublin, without action to strains of Escherichia coli. / Os frutos de Spondias purpurea L. (Anacardeacea), conhecidos popularmente como seriguela, s?o ricos em compostos fen?licos, que podem ser aplicados na produ??o de fitocosm?ticos. Este trabalho teve como objetivo analisar os constituintes qu?micos do extrato bruto metan?lico de S. purpurea(EBMSP) por cromatografia l?quida de alta efici?ncia acoplada ? espectrometria de massas (CLAE-ESI-EMn), avaliando sua utiliza??o como princ?pio ativo em formula??es fotoprotetoras. Em adi??o, o teor de fen?licos e de flavonoides, bem como a atividade de sequestro de radicais livres pelo m?todo de DPPH e atividade antimicrobiana pelo m?todo do halo de inibi??o, tamb?m foram verificados. A an?lise por CLAE-ESI-EMn possibilitou caracterizar cinco compostos fen?licos, entre eles ?cidos fen?licos, taninos e flavon?is. O extrato apresentou valores de teor de fen?licos e flavonoides de 28,68 ? 0,046 mg de equivalente a ?cido g?lico/g e 2,64 ? 0,005 mg de flavonoide equivalente a quercetina/g de extrato, respectivamente. A atividade antioxidante in vitro demonstrou percentual de inibi??o de 74,41 a 250?g/mL, valor elevadoquando comparado com o padr?o de rutina. A avalia??o do potencial fotoprotetor in vitro do EBMSP demonstrou atividade fotoprotetora, com valores de FPS>6,0 em baixas dilui??es, e prote??o UVA igual aos padr?es de benzofenona-3 e rutina. Os melhores resultados entre as formula??es de 5, 10, 20 e 30%, para o perfil fotoprotetor UVA e UVB, corresponderam ? lo??o de 30%, entretanto, nenhuma das formula??es obteve sinergismo com o padr?o de benzofenona-3. Para avalia??o da atividade antimicrobiana, sugere-se que o EBMSP possui atividade contra os micro-organismos Gram-negativos Pseudomonas aeruginosa e Salmonella dublin, sem a??o para cepas de Escherichia coli.