Dizertační práce pojednává o návrhu a optimalizaci periodických struktur s elektromagnetickým zádržným pásmem (EBG – electromagnetic band gap) pro potlačení povrchových vln šířících se na elektricky tlustých dielektrických substrátech. Nepředvídatelné chování elektromagnetických vlastností těchto struktur v závislosti na parametrech elementární buňky činí jejích syntézi značně komplikovanou. Bez patřičného postupu je návrh EBG struktur metodou pokusu a omylu. V první části práce jsou shrnuty základní poznatky o šíření elektromagnetických vln v tzv. metamateriálech. Následně je diskutován správný způsob výpočtu disperzního diagramu ve vybraných komerčních programech. Jádrem dizertace je automatizovaný návrh a optimalizace EBG struktur využitím různých globálních optimalizačních algoritmů. Praktický význam vypracované metodiky je předveden na návrhových příkladech periodických struktur s redukovanými rozměry, dvoupásmovými EBG vlastnostmi, simultánním EBG a AMC (artificial magnetic conductor – umělý magnetický vodič) chováním a tzv. superstrátu. Poslední kapitola je věnována experimentálnímu ověření počítačových modelů.

Tato dizertační práce se zabývá modelováním mikrovlnných polovodičových struktur. Stále vyšší pracovní kmitočty komunikačních systémů zvyšují nároky na aktivní prvky a přenosová vedení, realizované zpravidla v monolitické integrované podobě. Přenosová vedení s rozprostřeným zesílením představují perspektivní řešení nejen jako zesilovací prvky, ale také jako aktivní napáječe mikrovlnných antén. V součastné době neexistuje vhodný softwarový nástroj pro jejich efektivní simulaci a návrh. První část této práce je zaměřena na implementaci termodynamické formulace modelu driftu a difuze pro numerickou simulaci transportních procesů polovodičových struktur v komerčním programu COMSOL Multiphysics. Další část je věnována implementaci Gunnova jevu v makroskopické aproximaci a vypracování simulační procedury pro analýzu struktur využívajících tento jev. Poslední část je věnována návrhu a analýze aktivních přenosových vedení a zlepšení jejich vlastností.

Mgr. Jiří Unger The utilization of 3D computer reconstruction visualizations in archaeology Abstract of dissertation thesis The visual presentation is in the field of archaeology one of the main communication media for conveying information and interpreting it. The recent introduction of digital technologies has brought a whole new dimension to archaeological visualisations. As a result of this development, they become three-dimensional, virtual, multifunctional and interactive. Technological development moreover has not only influenced the way how to retrieve and process the data, but greatly expanded as well the ability to simulate and visualize their different aspects in virtual environment. Since the beginning the virtual archaeology has been taken as direction that would bring new groundbreaking knowledge into the archaeology. However, the current situation is that 3D computer visualizations are just popular conference theme than a standard tool of archaeology as a science, and most works on virtual archaeology still needs to advocate the benefits of 3D visualizations. Therefore, the dissertation thesis try to deal with theoretical issues of preparation, organisation and analysis of input data in the process of creation of 3D reconstruction computer models, then with possible solutions of visualization...

This thesis discusses the process of modeling a 21,000 seat fan-shaped auditorium using methods of geometric acoustics. Two commercial geometric acoustics software packages were used in the research: CATT-Acoustic™ 8.0 and EASE™ 4.1. The process first included creating preliminary models of the hall using published absorption coefficients for its surfaces and approximate scattering coefficients based on current best-known techniques. A detailed analysis determined the minimum numbers of rays needed in both packages to produce reliable results with these coefficient values. It was found that 100,000 rays were needed for CATT™ and 500,000 rays were needed for EASE™. Analysis was also done to determine whether the model was sensitive to the scattering coefficients of the seating areas. It was found that most acoustic parameters were not significantly affected by scattering coefficient variation. The models were subsequently refined by including measured absorption coefficients of dominant surfaces in the hall: the seats, audience and suspended absorptive panels. Comparisons were made between measurements made in the hall and results from the computer models with impulse responses, acoustic parameters, and auralizations. The results have shown that the models have been successful at representing characteristics of the hall at some positions but less successful at representing them at other positions. Comparisons have shown that positions on the rostrum were especially difficult positions to model in this hall. Significant differences were not found between the preliminary models and the refined models. There was not significant evidence showing that either the EASE™ or the CATT™ model was more successful in accurately representing the acoustical conditions of the hall. The results from this research suggest that more work must be done to improve the modeling capabilities of these packages for this application.

No description available.

No description available.

Le rôle des deux paires de bases universelles inverse Hoogsteen U : A ( RHUAs ) présentent chez les ARNt standards , une dans la boucle T et l'autre dans le noyau de la forme en L , a été étudiée. Pour chacun des RHUAs , un criblage génétique spécialisé in vivo chez les bactéries , le système suppresseur ambre ( pour l'étude de la RHUA dans la boucle T ) et le système d'ARNt de la sélénocystéine ( tRNASec ) ( pour l'étude de la RHUA dans le noyau ) , ont été utilisé pour générer des variants fonctionnels à partir de multiples librairies combinatoires . Ces variants ont ensuite été séquencé et soumis à une analyse systématique qui comprend la modélisation informatique et un type d'analyse phylogénétique. Les résultats du système suppresseur ambre ont montré un ensemble de variants fonctionnels qui ne nécessitent pas le motif RHUA dans la boucle T et qui ont remplacé la méthode standard de l'interaction entre les boucles D et T avec une double hélice interboucle , ILDH . D'autres études ont abouti à la détermination d'un modèle In silico de l'alternative à la norme standard de la boucle T, sous le nom de type III . Les résultats du système tRNASec ont révélé que pour cette ARNt exceptionnel, l'absence de RHUA ( dans le noyau ) assure une flexibilité accrue qui est spécifiquement nécessaire pour la fonction de tRNASec . Ainsi, les ARNt standards , à la différence de tRNASec , avec la présence universelle de RHUA dans le noyau , a été naturellement sélectionnée pour être rigide . Pris ensemble, la RHUA joue un rôle essentiel dans la stabilisation des interactions tertiaires. / The role of two universally present reverse Hoogsteen U:A base pairs (RHUAs) in the T-loop and in the core of the L-shape of standard tRNA was studied. To study each of the RHUAs, bacterial in vivo genetic screens were used including the amber suppressor system (for the study of the RHUA in the T-loop) and the selenocysteine tRNA(tRNASec) system (for the study of the RHUA in the core). These screens generated functional variants from multiple combinatorial libraries. These variants were subsequently sequenced and subjected to a systematic analysis which included computer modeling and a type of phylogenetic analysis. The results from the amber suppressor system showed a set of functional variants which did not require the RHUA motif in the T-loop, and had replaced the standard way of interaction between the D and T loops with an interloop double helix, ILDH. Further study culminated in the determination of an insilico model of the alternative to the standard T-loop known as type III. The results from the tRNASec system revealed that for this exceptional tRNA, the absence of RHUA (in the core) ensures an enhanced flexibility that is specifically required for tRNASec function. Thus standard tRNAs, unlike tRNASec, with the universal presence of RHUA in the core have been naturally selected to be rigid. Taken together, RHUA plays an essential role in the stabilization of tertiary interactions.

Para melhorar a visualização das pequenas estruturas anatômicas importantes para o diagnóstico do câncer de mama e otimizar o processamento das mamografias pelos sistemas computadorizados de auxílio ao diagnóstico (CAD), foi desenvolvido um dispositivo, baseado na tecnologia slit, que melhora a aquisição dos mamogramas. Este dispositivo reduz a radiação espalhada e o tamanho do foco e foi projetado a partir de um modelo computacional. O modelo adotado permite quantificar o valor desses parâmetros para qualquer sistema radiológico, qualquer espessura de tecidos moles radiografada e qualquer tensão aplicada ao tubo de raios-X. O dispositivo foi implementado e testado, quantificando as melhorias obtidas. As imagens geradas foram comparadas com as fornecidas pelos sistemas mamográficos convencionais. O modelo serviu para estudar também a interferência do espalhamento sobre o desempenho dos algoritmos usados nos sistemas de diagnóstico auxiliado por computador (CAD). / In order to improve the visualization of the small and important anatomical structures for breast cancer diagnosis and to optimize the image processing of mammograms by the computer aided diagnosis systems (CAD), a device that comes from slit technology was developed to improve the acquisition of mammograms. This device reduces the amount of scattered radiation and the focus size and it can be projected from a computational model. This model allows quantifying the value of those parameters, scattered radiation and the focus size, for any radiological system, any X-rayed soft tissue thickness and any tension applied to the X-ray tube. The device was implemented and tested quantifying the obtained improvements. The generated images were compared to the supplied for conventional mammography systems. The model was also good for studying interference on the algorithms used in the computer aided diagnosis systems (CAD).

<p>Neuropeptide Y (NPY) is known to influence a vast number of physiological and behavioral processes such as vasoconstriction, circadian rhythms, feeding, anxiety and memory. Peptides of the NPY family bind to five different cloned G-protein coupled receptor subtypes (Y1, 2, 4-6). The studies compiled in this thesis present inter-species comparisons of sequence similarities, binding properties and expression patterns among receptors of the NPY family.</p><p>Cloning of Y1 and Y2 receptor subtypes from guinea pigs revealed strong binding profile similarity to the corresponding human receptors. Previously demonstrated atypical binding profiles in the caval vein of guinea pigs were concluded to result from other receptors than the cloned Y1 and Y2 receptors, or possibly combinations of distinct receptor subtypes.</p><p>The guinea pig Y5 receptor was found to be expressed in regions of the brain that have been indicated as important for regulation of food intake. Expression in the hypothalamus, amygdala and brain stem was noticed, similar to studies in rats and humans. In other brain regions, such as the striatum and hippocampus, some species differences were observed.</p><p>Mutagenesis studies of the human Y1 receptor indicated sites important for binding both of endogenous agonists and synthetic antagonists. Putative new sites of interaction with the Y1 antagonists BIBP3226 and/or SR120819A were recognized. The data were used to construct a three-dimensional structure model, based on a high-resolution bovine rhodopsin model.</p><p>Cloning of the chicken (<i>Gallus gallus</i>) Y1, Y2 and Y5 receptors revealed high sequence similarities with mammalian receptors. Most endogenous ligands bound with similar affinities as to mammalian receptors. The strongest exception was the discovery of high-affinity binding to chicken Y2 of [Leu³¹, Pro³⁴]NPY, which was previously considered to bind non-Y2 receptors only. </p><p>The new human Y1 receptor model provides a basis for further investigations of ligand-receptor interactions which will be aided by information on NPY receptors from other taxa. Guinea pigs are concluded to be a good complement to rats and mice for studying NPY signaling. These results demonstrate the benefits of species comparisons for pharmacological studies.</p>

Neuropeptide Y (NPY) is known to influence a vast number of physiological and behavioral processes such as vasoconstriction, circadian rhythms, feeding, anxiety and memory. Peptides of the NPY family bind to five different cloned G-protein coupled receptor subtypes (Y1, 2, 4-6). The studies compiled in this thesis present inter-species comparisons of sequence similarities, binding properties and expression patterns among receptors of the NPY family. Cloning of Y1 and Y2 receptor subtypes from guinea pigs revealed strong binding profile similarity to the corresponding human receptors. Previously demonstrated atypical binding profiles in the caval vein of guinea pigs were concluded to result from other receptors than the cloned Y1 and Y2 receptors, or possibly combinations of distinct receptor subtypes. The guinea pig Y5 receptor was found to be expressed in regions of the brain that have been indicated as important for regulation of food intake. Expression in the hypothalamus, amygdala and brain stem was noticed, similar to studies in rats and humans. In other brain regions, such as the striatum and hippocampus, some species differences were observed. Mutagenesis studies of the human Y1 receptor indicated sites important for binding both of endogenous agonists and synthetic antagonists. Putative new sites of interaction with the Y1 antagonists BIBP3226 and/or SR120819A were recognized. The data were used to construct a three-dimensional structure model, based on a high-resolution bovine rhodopsin model. Cloning of the chicken (Gallus gallus) Y1, Y2 and Y5 receptors revealed high sequence similarities with mammalian receptors. Most endogenous ligands bound with similar affinities as to mammalian receptors. The strongest exception was the discovery of high-affinity binding to chicken Y2 of [Leu31, Pro34]NPY, which was previously considered to bind non-Y2 receptors only. The new human Y1 receptor model provides a basis for further investigations of ligand-receptor interactions which will be aided by information on NPY receptors from other taxa. Guinea pigs are concluded to be a good complement to rats and mice for studying NPY signaling. These results demonstrate the benefits of species comparisons for pharmacological studies.