Antibiotics are routinely used in modern livestock production systems to treat and prevent disease as well as to enhance livestock production and/or outputs. This widespread use of antibiotics has led to a growing worldwide interest in antibioticfree animal production. The addition of feed supplements such as nucleotides to improve intestinal health as well as the early development of the immune system needs to be investigated as an environmentally-friendly nutritional management activity. In this study four (4) groups of newborn Holstein calves (n=24) were evaluated after being treated as follows: Group 1 (Negative control), Group 2 (Investigational Veterinary Product (IVP) 1 – Oral supplement containing nucleotides, vitamins, essential fatty acids, essential amino acids, pre-biotics and trace-minerals), Group 3 (IVP 2 – Oral supplement containing nucleotides only at concentration and dosage equal to IVP1) and Group 4 (IVP 3 - Parenteral glucocorticoids). Results of the study indicated that: Pre-colostral Serum IgG titres – After titration of the serum the anti-BVDV results showed no difference between the study groups. All calves recorded a SP ratio of zero value (0.00 ± 0.2) indicating that they had not been nursed by their mothers prior to the start of the study. This was an entrance requirement for the calves to be enrolled into the study. Apparent Efficiency of Absorption % (AEA%) – Literature shows that an AEA% result of between 20%-30% is good and 35% is excellent. The AEA% recorded for this study fell within this range although there was no statistically significant difference shown between the groups. In spite of a strictly controlled study protocol, 7 of the 24 calves (29%) still suffered from FPT in this study. The result falls within the range of 19% - 37% reported on United States of America (USA) farms by Doepel and Bartier in 2014. Serum cortisol - When compared to the control group, there were no statistical significant difference evident for group 2 and group 3. However, the difference between the Glucocorticoid – Group 4 and the control and other groups was statistically significant (P = 0.0001; table 12) at the various time intervals. It was also evident that the inclusion of parenteral glucocorticoids (group 4) may have assisted in prolonging the natural “gut closure”. Gut closure – a positive 2 point linear regression forecast line indicated an increasing trend in IgG absorption post-24 hours for group number 4 whilst groups 1, 2 and 3 each had a negative 2 point linear regression forecast line (figure 23). Weight / ADG (D42) - Group 2 showed a statistically significant increase in mean D42 weight (P = 0.0042) of 59.167 kg ± 3.545 kg when compared to the other study groups and compared to the control group (P = 0.0227). A comparison of the relative increase in mass of groups indicated that Group 2 efficiently achieved this result with a statistically significant ADG of 0.536 kg (P = 0.014) compared to the other study groups and compared to the control group (P = 0.022). In addition, weight / FCR – FCR was calculated for all groups as follows: G1 – 4.000 kg, G2 - 2.593 kg, G3 – 2.703 kg and G4 - 3.012 kg feed required for the production of 1 kg meat. The results indicated that the group 2 feed input was the most economical followed by groups 3, 4 and control respectively. In conclusion, it is evident that a nutritional supplement containing nucleotides, vitamins, trace- minerals, essential fatty acids, essential amino acids and pre-biotics may contribute significantly to important economic indicators such as weight gain, ADG and FCR on a commercial dairy farm. / Environmental Sciences / Ph. D. (Environmental Science)

An immunoglobulin-fortified milk replacer and a subcutaneous (SC) injectable solution of immunoglobulins (Ig) were examined as methods of achieving passive immunity in neonatal calves. Bovine Ig, from abattoir blood, were purified by polyphosphate fractionation and ion-exchange chromatography. In experiment 1, carried out at Agriculture Canada Research Station, Agassiz, 37 colostrum-deprived Holstein-Friesian bull calves were allotted to one of four treatments. Col/WM calves were fed colostrum on day 1 and whole milk, days 2 - 42. MR-Nolg calves (control) were fed milk replacer with no Ig, days 1-42. MR-Hi/Lo calves were fed milk replacer with Ig at 50 mg/ml on day 1, and at 10 mg/ml, days 2 -21. MR-Hi/No calves were fed milk replacer with Ig at 50 mg/ml, day 1,'and with no Ig, days 2 - 21. From days 2 2 - 42, MR-Hi/Lo and MR-Hi/No treatment calves received milk replacer with no Ig. In experiment 2, carried out at the University Research Farm at Oyster River, 24 colostrum-deprived Holstein-Friesian bull calves were allotted to one of three treatments. The first two treatments were the same as for experiment 1, Col/WM and MR-Nolg fed for days 1-21. MR-Lo Inj calves were fed milk replacer with Ig at 10 mg/ml, days 1-21, and were also given a SC injection of Ig solution within the first 6 hours of life. For days 2-42, calves were fed WM or MR-Nolg, as per experiment 1. For both experiments, blood samples and calf weights were taken at birth, 24 & 48 hours of age, day 7 and weekly thereafter for six weeks. Diarrhea (scours) levels, rectal temperatures and general health of calves were recorded daily for the first three weeks as well. Experiment 1 survival at 6 weeks of age was 11 out of 11 calves for Col/WM treatment, 8 out of 8 calves for MR-Hi/Lo treatment, 7 out of 8 calves for MR-Hi/No and a significantly lower (P>0.05) 7 out of 9 calves for MR-Nolg. In experiment 2, survival was 7 out of 8 calves for both Col/WM and MR-Lo-Inj treatments and a significantly lower (P>0.05) 4 out of 8 calves for MR-Nolg treatment. Calves on MR-Hi/No had significantly higher diarrhea levels than the other three treatments over weeks one and four in experiment 1. In experiment 2, calves which did not receive any Ig had significantly higher diarrhea levels over weeks three and four than calves which received Ig. Experiment 1 average daily gains (ADG) were significantly higher for calves on Col/WM, MR-Hi/Lo and MR-Hi/No treatments than for calves on MR-Nolg at six weeks of age. In experiment 2, six week ADG were significantly higher for calves on Col/WM and MR-Lo Inj treatments than for MR-Nolg. For both experiments, serum Ig levels of calves on Col/WM were significantly higher than calves on the other treatments at 24 and 48 hours of age. MR-Hi/Lo, MR-Hi/No and MR-Lo Inj calves trended to higher serum Ig levels than MR-Nolg calves but were not significantly different. Calves which received Ig, from colostrum, the Ig-fortified milk replacer or a subcutaneous Ig injection, had higher survival rates, lower diarrhea levels, less antibiotic treatment and higher average daily gains than calves hot receiving any Ig. It was concluded that immunoglobulins, administered either orally or parenterally, are an effective, alternative method, for providing passive immunity in neonatal calves. / Land and Food Systems, Faculty of / Graduate

Holstein calves were photographed and measured over a period of 84 days to determine the feasibility of Image Analysis as a measurement tool. It was determined that the disparity between actual measurements and image analysis measurements decreased as the bone length increased, and that image analysis could be used to monitor growth successfully in large ungulates, using the length of certain bone. Image analysis was then used to evaluate growth in Holstein calves on two weaning programs (weaned early at six weeks of age and weaned later at eight weeks of age). Calves were weighed and photographed over a period of 224 days to determine the effects of weaning on skeletal symmetry. Weaning time did have an affect on the skeletal symmetry of the calves, with calves weaned earlier found to be more asymmetrical. The extent to which skeletal symmetry is affected by a stress is determined by the nature and duration of the stress. The growth and development of Thoroughbred foals was recorded and photographed for a period of 300 days and the relative asymmetry for bilateral traits was assessed. Thoroughbred foal growth correlated to statistics reported over the last twenty eight years. Asymmetry can be detected in growing foals using image analysis. Weaning stress produced some asymmetry that the foals were able to recover from. One can use highly correlated body weight and height measurements to produce a tool stud managers can used to monitor growth. In young training Thoroughbreds (between 18 and 24 months of age), no significant asymmetry was found in the forelimbs but the hind legs displayed asymmetry, which has interesting implications for training and for performance criteria in the racehorse. Tools can be produced to monitor the growth and development of Thoroughbred foals destined to race, which can improve their management and the duration of their racing careers. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Twenty four Jersey calves were randomly blocked according to sex and birth date, to determine the effects of supplementing Carica papaya seed (Linn) meal (CPSM) on health and growth performance of calves. In the first study, calves were fed CPSM for only 2 days to determine faecal pathogen population. In the second study; calves were randomly assigned at birth to three treatments and fed until weaning at 42 days. Each treatment had six calves. Treatments were; 1) a control group, which did not receive additive; 2) a group that was supplemented 5 g/d of Carica papaya seed meal (CPSM) and 3) the last group that was supplemented 5 g/d of a commercial product containing Lactobacillus acidophilus (Lact). After receiving milk with colostrum from their dams for 3 consecutive days after birth, calves had commercial starter pellet and fresh water ad lib. Treatments were added to whole milk from day 4 and fed to calves before being allowed to suckle from the dams for 30 min three times a day (08:00; 12:00 and 17:00). Calf starter dry matter intake (DMI) was evaluated daily while body weight (BW) and body structural growth were measured weekly. Faecal samples were collected directly from the rectum on day 7 and 10, before and after receiving CPSM respectively. The DMI; DMI/BW; BW and BWG of calves did not differ among treatments, and averaged 305.4 g/d; 7.7 g/d per BW; 39.4 kg and 32.1 kg, respectively. The initial BW, 22.2 ± 1.49 kg did not differ among groups, but the weaning BW was higher (p<0.05) for CPSM fed calves compared to control diet fed calves. It did not differ between control and Lact calves. Calves in Lact and CPSM treatments had similar average daily gain (ADG) and heart girth (HG), which were higher (p<0.05) than calves fed the control treatment. Calves fed CPSM had higher (p<0.05) hip width (HW) and shoulder height (SH) than control calves. There were effects of time (p<0.001) for starter DMI/BW, ADG and all structural body parameters, and effects (p<0.05) of interaction between time and treatments for only starter DMI/BW, ADG and HG. The CPSM treatment reduced faecal coliforms and E. coli (p<0.05) by 93.6% and 96.1%, respectively; and tended to reduce Enterobacteriaceae (p=0.056) by 96.4%. The present study revealed that feeding CPSM to calves during the pre-weaning period increased growth performance by improving average daily gain, feed efficiency and enhancing health status due to low faecal pathogen count / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

Department of Animal Science / MSCAGR (Animal Science)

Bovine maternal antibodies are not transferred across the placenta during pregnancy and newborn calves are unable to produce their own antibodies within the first weeks after birth. As neonates are born agammaglobulinemic they need to acquire immunoglobulins (Ig) from their dam’s colostrum to acquire passive immunity. Colostrum fed to dairy calves, which is not timeous, of inadequate quantity and of unverified quality, might result in decreased neonate health. The aim of this study was to conduct a survey of the management of colostrum on commercial dairy farms, to estimate the quality of the Ig titre of colostrum fed to neonates and finally to recommend methods and techniques critical to the successful management of colostrum. The methods used included a questionnaire which was conducted as a structured interview on a one-on-one basis among 50 randomly selected commercial dairy farmers in the Eastern Cape Coastal Region of South Africa. The estimation of the colostrum Ig titre of colostrum fed to neonates was made by the on-farm measurement of specific gravity (SG) by making use of a commercially available KRUUSE colostrometer (Fleenor and Stott, 1980). A pooled colostrum sample, from each of the four quarters, from 90 randomly selected post-partum cows was collected on a leader commercial dairy farm. This method was used to compare colostrum samples from cows run under similar management practices. These samples were collected for analysis within 6 hrs of calving and were done over 3 seasons (autumn, winter and spring). Survey - The colostrum mass and timing of the initial feed are the most important factors when aiming to achieve adequate passive immunity. The results of the survey indicated that most of the farmers of this region feed an inadequate mass of colostrum (volume and Ig concentration) and only 52% of farmers surveyed feed colostrum less than 6 hrs post- partum. The majority (78%) of surveyed farmers did not follow up their initial colostrum feeding.Colostrum sampling - At the trial site only 10% (9 from 90 colostrum samples measured), were found to be of adequate SG quality. Cow age (parity), season of calving and colostrum temperature had an influence on the estimated colostrum SG. However, season of calving was found to have the greatest influence on SG values. These results were consistent with findings from previous studies that SG values from the cooler months were higher than those of the hotter months. Tables 4.7 (P=0.330), 4.8 (P=0.012) and 4.9 (P=0.005) showed that regression analysis confirmed that LS means across seasons were inadequately below the required 50 mg/ml Ig required for sufficient passive immunity. Tables 4.1 (P=0.164), 4.2 (P=0.011) and 4.3 (P=0.021) shows that season of calving had a much greater effect on CR than did parity, Table 4.5 (P=0.177). Table 4.4 shows that colostrum temperature has an significant effect on SG value. Recommendations for methods and techniques critical to the successful management of colostrum were made. These recommendations were based on the analysis of the data obtained from the questionnaire and the on-farm colostrum sampling study. The most important and critical management practices surveyed includes the timing of the cow and calf separation where it was found that only 30 from the 50 (60%) of the farms surveyed separate calves and dams at day (0), 19 from 50 farms (38%) separate at day (3-5) and 1 from 50 farms (2%) separate only at day 7 or later. Thus 40% of surveyed farms allow cows to nurse their calves. With regards to early exposure to pathogens this is a high risk management practice. Further to that, only 2 from 50 surveyed farms (4%) measure the colostrum quality fed to their calves and 48 from 50 farms (96%) feed colostrum of unmeasured quality. The mass of colostrum fed to calves is an important parameter for successful transmission of Ig. In the survey it was found that 28 from 50 farms (56%) feed 2L – 4L of colostrum and 11 from 50 farms (22%) feed 2L of colostrum. Thus 78% of farms feed approximately 50% of the amount of colostrum required for successful transmission of Ig. Finally only 1 from 50 farms (2%) freeze excess colostrum and 1 from 50 farms (2%) pool excess colostrum. Both these farms measure colostrum quality by colostrometer. / Agriculture Animal Health and Human Ecology / M. Sc. (Agriculture)

Bovine maternal antibodies are not transferred across the placenta during pregnancy and newborn calves are unable to produce their own antibodies within the first weeks after birth. As neonates are born agammaglobulinemic they need to acquire immunoglobulins (Ig) from their dam’s colostrum to acquire passive immunity. Colostrum fed to dairy calves, which is not timeous, of inadequate quantity and of unverified quality, might result in decreased neonate health. The aim of this study was to conduct a survey of the management of colostrum on commercial dairy farms, to estimate the quality of the Ig titre of colostrum fed to neonates and finally to recommend methods and techniques critical to the successful management of colostrum. The methods used included a questionnaire which was conducted as a structured interview on a one-on-one basis among 50 randomly selected commercial dairy farmers in the Eastern Cape Coastal Region of South Africa. The estimation of the colostrum Ig titre of colostrum fed to neonates was made by the on-farm measurement of specific gravity (SG) by making use of a commercially available KRUUSE colostrometer (Fleenor and Stott, 1980). A pooled colostrum sample, from each of the four quarters, from 90 randomly selected post-partum cows was collected on a leader commercial dairy farm. This method was used to compare colostrum samples from cows run under similar management practices. These samples were collected for analysis within 6 hrs of calving and were done over 3 seasons (autumn, winter and spring). Survey - The colostrum mass and timing of the initial feed are the most important factors when aiming to achieve adequate passive immunity. The results of the survey indicated that most of the farmers of this region feed an inadequate mass of colostrum (volume and Ig concentration) and only 52% of farmers surveyed feed colostrum less than 6 hrs post- partum. The majority (78%) of surveyed farmers did not follow up their initial colostrum feeding.Colostrum sampling - At the trial site only 10% (9 from 90 colostrum samples measured), were found to be of adequate SG quality. Cow age (parity), season of calving and colostrum temperature had an influence on the estimated colostrum SG. However, season of calving was found to have the greatest influence on SG values. These results were consistent with findings from previous studies that SG values from the cooler months were higher than those of the hotter months. Tables 4.7 (P=0.330), 4.8 (P=0.012) and 4.9 (P=0.005) showed that regression analysis confirmed that LS means across seasons were inadequately below the required 50 mg/ml Ig required for sufficient passive immunity. Tables 4.1 (P=0.164), 4.2 (P=0.011) and 4.3 (P=0.021) shows that season of calving had a much greater effect on CR than did parity, Table 4.5 (P=0.177). Table 4.4 shows that colostrum temperature has an significant effect on SG value. Recommendations for methods and techniques critical to the successful management of colostrum were made. These recommendations were based on the analysis of the data obtained from the questionnaire and the on-farm colostrum sampling study. The most important and critical management practices surveyed includes the timing of the cow and calf separation where it was found that only 30 from the 50 (60%) of the farms surveyed separate calves and dams at day (0), 19 from 50 farms (38%) separate at day (3-5) and 1 from 50 farms (2%) separate only at day 7 or later. Thus 40% of surveyed farms allow cows to nurse their calves. With regards to early exposure to pathogens this is a high risk management practice. Further to that, only 2 from 50 surveyed farms (4%) measure the colostrum quality fed to their calves and 48 from 50 farms (96%) feed colostrum of unmeasured quality. The mass of colostrum fed to calves is an important parameter for successful transmission of Ig. In the survey it was found that 28 from 50 farms (56%) feed 2L – 4L of colostrum and 11 from 50 farms (22%) feed 2L of colostrum. Thus 78% of farms feed approximately 50% of the amount of colostrum required for successful transmission of Ig. Finally only 1 from 50 farms (2%) freeze excess colostrum and 1 from 50 farms (2%) pool excess colostrum. Both these farms measure colostrum quality by colostrometer. / Agriculture Animal Health and Human Ecology / M. Sc. (Agriculture)