Tsang Po Ting. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 135-152). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abstract 摘要 --- p.iv / List of Figures --- p.vi / List of Tables --- p.viii / List of Abbreviations --- p.ix / Table of Contents --- p.xii / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter 1.1 --- The Role of Glucose in Biological System --- p.1 / Chapter 1.2 --- Glucose Transporter Families --- p.1 / Chapter 1.2.1 --- Na+-Dependent Glucose Transporters --- p.2 / Chapter 1.2.2 --- Facilitative Glucose Transporters --- p.3 / Chapter 1.3 --- Glucose Transporter Type1 --- p.7 / Chapter 1.3.1 --- Primary Structure of the Glutl Protein --- p.7 / Chapter 1.3.2 --- Secondary Structure --- p.8 / Chapter 1.3.3 --- Tertiary Structure --- p.8 / Chapter 1.3.4 --- Kinetics Properties --- p.11 / Chapter 1.3.5 --- Tissue Distribution --- p.12 / Chapter 1.3.6 --- Multifunctional Property --- p.13 / Chapter 1.3.7 --- Characterization of GLUT1 Gene --- p.13 / Chapter 1.3.8 --- Regulation of GLUT1 Expression --- p.14 / Chapter 1.4 --- Glucose Transporter Type 1 and the Brain --- p.16 / Chapter 1.5 --- Glucose Transporter Type 1 Deficiency Syndrome (GIutlDS) --- p.19 / Chapter 1.5.1 --- Backgronnd of GIutlDS --- p.19 / Chapter 1.5.2 --- Clinical Features of GIutlDS --- p.23 / Chapter 1.5.3 --- Genotype-Phenotype Correlations --- p.24 / Chapter 1.5.4 --- Diagnosis --- p.26 / Chapter 1.5.5 --- Manage nent --- p.27 / Chapter 1.5.5.1 --- Ketogenic Diet --- p.27 / Chapter 1.6 --- Hypothesis and Objectives --- p.29 / Chapter Chapter 2: --- Biochemical and Molecular Analysis of GLUT1 in a Suspected GlutlDS Case --- p.31 / Chapter 2.1 --- Materials --- p.32 / Chapter 2.1.1 --- Clinical History of Suspected GlutlDS Patient --- p.32 / Chapter 2.1.2 --- Blood Samples --- p.32 / Chapter 2.1.3 --- Reagents and Buffers for Reverse Transcription --- p.32 / Chapter 2.1.4 --- Reagents and Buffers for TA Cloning --- p.34 / Chapter 2.1.5 --- Reagents for Genomic DNA Extraction --- p.34 / Chapter 2.1.6 --- Reagents and Buffers for Polymerase Chain Reaction (PCR) --- p.34 / Chapter 2.1.7 --- Reagents and Buffers for Agarose Gel Electrophoresis --- p.35 / Chapter 2.1.8 --- Reagents for Zero-trans 3-OMG Influx in Erythrocytes --- p.37 / Chapter 2.1.9 --- Reagents for Zero-trans 3-OMG Efflux from Erythrocytes --- p.38 / Chapter 2.1.10 --- Reagents for Erythrocytes Membrane Extraction and Detection --- p.39 / Chapter 2.2 --- Methods --- p.44 / Chapter 2.2.1 --- GLUT1 Gene Analysis --- p.44 / Chapter 2.2.2 --- Zero-trans 3-OMG Influx into Erythrocytes --- p.51 / Chapter 2.2.3 --- Zero-trans 3-OMG Efflux from Erythrocytes --- p.52 / Chapter 2.2.4 --- Glutl Protein Expression --- p.54 / Chapter 2.2.5 --- Statistics --- p.57 / Chapter 2.3 --- Results --- p.58 / Chapter 2.3.1 --- Molecular Analysis of the GLUT1 Gene of a Suspected GlutlDS Patient --- p.58 / Chapter 2.3.2 --- Functional Analysis of the GlutlDS Patient's Glutl Protein --- p.61 / Chapter 2.3.3 --- Glutl Protein Expression in the GlutlDS Patient --- p.64 / Chapter 2.4 --- Discussion --- p.66 / Chapter Chapter 3: --- Pathogenicity Studies of GLUT1 Mutations --- p.71 / Chapter 3.1 --- Materials --- p.72 / Chapter 3.1.1 --- Construction of Glutl-Encoding Vectors --- p.72 / Chapter 3.1.2 --- Cell Lire --- p.73 / Chapter 3.1.3 --- "Cell Culture Media, Buffers and Other Reagents" --- p.73 / Chapter 3.1.4 --- Cell Culture Wares --- p.75 / Chapter 3.1.5 --- Reagents for Transfection --- p.75 / Chapter 3.1.6 --- Reagents for Protein Determination and Western Blot Analysis --- p.76 / Chapter 3.1.7 --- Consumables for Confocal Microscopy --- p.77 / Chapter 3.1.8 --- Reagents and Buffers for Flow Cytometry --- p.77 / Chapter 3.1.9 --- Reagents for 2-DOG Uptake in CHO-K1 Cells --- p.77 / Chapter 3.2 --- Methods --- p.79 / Chapter 3.2.1 --- Cell Culture Methodology --- p.79 / Chapter 3.2.2 --- Construction of GLUT1 Mutants --- p.80 / Chapter 3.2.3 --- Establishment of Wild Type and Mutant Glutl Expressing Cell Lines --- p.84 / Chapter 3.2.4 --- Protein Expression Study --- p.85 / Chapter 3.2.5 --- 2-DOG Influx Assay in CHO-K1 Cells --- p.87 / Chapter 3.2.6 --- Confocal Microscopy Studies on Glutl Cellular Localization --- p.89 / Chapter 3.2.7 --- Statistics --- p.90 / Chapter 3.3 --- Results --- p.91 / Chapter 3.3.1 --- Molecular Analysis of 1034-1035Insl2 Mutation --- p.91 / Chapter 3.3.2 --- Expression of the Wild Type and Mutant GFP-Glutl Fusion Proteins --- p.92 / Chapter 3.3.3 --- Functional Analysis of the 1034-1035Insl2 Mutant --- p.95 / Chapter 3.4 --- Discussion --- p.97 / Chapter Chapter 4: --- GLUT1 Promoter Study --- p.100 / Chapter 4.1 --- Materials --- p.101 / Chapter 4.1.1 --- Construction of GLUT1 Promoter Vectors --- p.101 / Chapter 4.1.2 --- Cell Lines --- p.102 / Chapter 4.1.3 --- Cell Culture Media and Other Reagents --- p.103 / Chapter 4.1.4 --- Dual Luciferase Reporter Assay System --- p.103 / Chapter 4.2 --- Methods --- p.105 / Chapter 4.2.1 --- Bioinformatics --- p.105 / Chapter 4.2.2 --- Cell Culture --- p.105 / Chapter 4.2.3 --- Construetion of GLUT1 Promoter Vectors --- p.105 / Chapter 4.2.4 --- 5'-Deletion Analysis of GLUT1 Promoter --- p.108 / Chapter 4.2.5 --- Determination of the Activities of GLUT1 Promoter Fragments --- p.110 / Chapter 4.2.6 --- Statistics --- p.113 / Chapter 4.3 --- Results --- p.114 / Chapter 4.3.1 --- Determination of the Promoter Activity of the 5'-deletion Fragments --- p.114 / Chapter 4.3.2 --- Prediction of Transcription Factors in the 5'-deletion Fragments --- p.119 / Chapter 4.4 --- Discussion --- p.121 / Chapter Chapter 5: --- General Conclusion and Future Perspectives --- p.133 / References --- p.135

Au Yeung, Wan Kin. / "August 2011." / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 89-103). / Abstracts in English and Chinese. / Thesis committee --- p.i / Statement --- p.ii / Abstract --- p.iii / Acknowledgements --- p.v / General abbreviations --- p.vi / Abbreviations of chemicals --- p.vii / List of figures --- p.ix / List of figures in Appendix VI --- p.xii / List of tables --- p.xiv / Table of Contents --- p.xv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- The significance of studying rice disease resistance --- p.1 / Chapter 1.1.1 --- Economic importance of rice --- p.1 / Chapter 1.1.2 --- Diseases caused by pathogens virulent to rice --- p.1 / Chapter 1.1.2.1 --- Bacterial leaf blight diseases --- p.1 / Chapter 1.1.2.2 --- Fungal blast diseases --- p.2 / Chapter 1.1.3 --- Approach to enhance resistance of crops towards pathogens --- p.2 / Chapter 1.2 --- Literature review on plant immunity system --- p.3 / Chapter 1.2.1 --- Pathogen associated molecular patterns (PAMP) and PAMP -triggered immunity (PTI) --- p.4 / Chapter 1.2.2 --- Pathogen effectors and effector-triggered immunity (ETI) --- p.5 / Chapter 1.2.3 --- Roles of phytohormones in plant defense responses --- p.6 / Chapter 1.2.4 --- G protein signaling and plant defense responses --- p.9 / Chapter 1.3 --- Literature review on FK506 binding proteins (FKBPs) --- p.10 / Chapter 1.4 --- Background information of this study - origin of the clone chosen for study in this project --- p.11 / Chapter 1.5 --- Hypothesis and Objectives --- p.12 / Chapter Chapter 2 --- Materials and Methods --- p.13 / Chapter 2.1 --- Materials --- p.13 / Chapter 2.1.1 --- "Plants, bacterial strains and vectors" --- p.13 / Chapter 2.1.2 --- Chemicals and Regents --- p.18 / Chapter 2.1.3 --- Commercial kits --- p.18 / Chapter 2.1.4 --- Primers and Adaptors --- p.19 / Chapter 2.1.5 --- Equipments and facilities used --- p.23 / Chapter 2.1.6 --- "Buffer, solution, gel and medium" --- p.23 / Chapter 2.2 --- Methods --- p.24 / Chapter 2.2.1. --- Bacterial and yeast cultures --- p.24 / Chapter 2.2.2 --- Plant growth conditions and treatments --- p.25 / Chapter 2.2.2.1 --- Surface sterilization of J. thaliana seeds --- p.25 / Chapter 2.2.2.2 --- Environmental conditions of A. thaliana for germination of seeds and growing of seedlings --- p.26 / Chapter 2.2.2.3 --- Environmental conditions of A. thaliana for growing of plants --- p.26 / Chapter 2.2.2.4 --- Pathogen inoculation test of A. thaliana with Pst DC3000 --- p.27 / Chapter 2.2.3 --- Cloning and subcloning of OsFKBP 12 and OsUCCl --- p.27 / Chapter 2.2.3.1 --- Sub-cloning of OsFKBP12 to pGEX-4T-l and pMAL-c2 --- p.27 / Chapter 2.2.3.2 --- Cloning of OsUCCl to pGEX-4T-l --- p.29 / Chapter 2.2.4 --- "DNA, RNA and protein extractions" --- p.29 / Chapter 2.2.4.1 --- Plasmid extraction from bacterial cells --- p.29 / Chapter 2.2.4.2 --- Genomic DNA extraction from plant through CTAB method --- p.29 / Chapter 2.2.4.3 --- RNA extraction from plant tissues --- p.30 / Chapter 2.2.4.4 --- Protein extraction from plant tissues --- p.31 / Chapter 2.2.4.5 --- Fusion protein extraction from E. coli --- p.31 / Chapter 2.2.5 --- Western blot analyses --- p.32 / Chapter 2.2.5.1 --- Western blot analysis of GST tag and MBP tag fusion proteins --- p.32 / Chapter 2.2.5.2 --- Western blot analysis native OsYchFl proteins --- p.33 / Chapter 2.2.6 --- Real-time PCR study --- p.33 / Chapter 2.2.6.1 --- cDNA synthesis --- p.33 / Chapter 2.2.6.2 --- Real-time PCR --- p.34 / Chapter 2.2.7 --- Yeast two hybrid --- p.35 / Chapter 2.2.7.1 --- Screening of OsFKBP 12 interaction protein partners by yeast mating --- p.35 / Chapter 2.2.7.2 --- Identification of positive interacting protein partners by extracting DNA plasmid from yeast --- p.35 / Chapter 2.2.7.3 --- Re-transformation of pGBKTl-OsFKBP 12 with their interacting partner clones into yeast (AH 109) by co-transformation --- p.36 / Chapter 2.2.8 --- In vitro pull down assay of OsFKBP 12 with their putative protein interacting partner --- p.36 / Chapter 2.2.8.1 --- In vitro pull down of native OsYchFl by MBP-His-OsFKBP12 --- p.36 / Chapter 2.2.8.2 --- In vitro pull down of GST-AtYchF 1 by MBP-His-OsFKBP12 --- p.37 / Chapter 2.2.8.3 --- In vitro pull down of MBP-His-OsFKBP12 by GST-OsUCCl --- p.37 / Chapter 2.2.8.4 --- In vitro pull down of MBP-His-OsFKBP12 by GST-OsYchFl G domain --- p.38 / Chapter 2.2.9 --- GTPase assay ofOsYchF with OsFKBP12 --- p.38 / Chapter 2.3.0 --- Phylogenetic analysis and sequence alignment --- p.39 / Chapter Chapter 3 --- Results --- p.40 / Chapter 3.1 --- Identification of OsFKBP 12 encoding a FKBP (FK506 binding protein)-domain containing protein in Oryza sativa (rice) --- p.40 / Chapter 3.2 --- OsFKBP12 was down-regulated in the pathogen-inoculated Xal4 rice line CBB14 --- p.47 / Chapter 3.3 --- Ecotpic expression of OsFKBP 12 repressed the expression of defense marker genes in transgenic A. thaliana --- p.50 / Chapter 3.4 --- Expressing OsFKBP 12 in transgenic A. thaliana enhanced the susceptibility to the bacterial pathogen Pst DC3000 --- p.54 / Chapter 3.5 --- OsFKBP 12 protein interacted with a putative defense-related G-protein and a copper binding protein --- p.57 / Chapter 3.6 --- "OsFKBP 12 protein interacted with the G domain of defense-related G protein, OsYchFl" --- p.69 / Chapter 3.7 --- OsFKBP 12 protein enhanced the in vitro phosphate release of OsYchFl --- p.72 / Chapter Chapter 4 --- Discussion --- p.74 / Chapter 4.1 --- The identification and characterization of OsFKBP 12 --- p.74 / Chapter 4.2 --- Expression pattern of OsFKBP 12 upon biotic stress in bacterial blight resistant near isogenic line (NIL) --- p.75 / Chapter 4.3 --- OsFKBP 12 repressed the expression of SA-regulated defense marker genes when ectopically expressed in A. thaliana --- p.75 / Chapter 4.4 --- Ectopic expression of OsFKBP 12 enhanced susceptibility towards Pst DC3000 in transgenic A. thaliana --- p.76 / Chapter 4.5 --- The interacting partners of OsFKBP 12 in relation to plant defense response --- p.78 / Chapter 4.6 --- The specific biochemical interaction of OsFKBP 12 with OsYchFl --- p.80 / Chapter 4.7 --- Future perspectives --- p.85 / Chapter Chapter 5 --- Conclusion --- p.87 / References --- p.89 / Appendix --- p.104

Yiu Wai Han. / "June 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 146-156) / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.