Spelling suggestions: "subject:"cmutation (biology)"" "subject:"cmutation (ciology)""
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Likelihood methods to infer balancing selection under K-allele models /Buzbas, Erkan Ozge. January 1900 (has links)
Thesis (Ph. D., Bioinformatics and Computational Biology)--University of Idaho, May 2009. / Major professor: Paul Joyce. Includes bibliographical references (leaves 58-63). Also available online (PDF file) by subscription or by purchasing the individual file.
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Identification and characterization of a new promoter in nin 5 mutation of bacteriophage lambda.January 1974 (has links)
Thesis (M. Ph.)--Chinese University of Hong Kong. / Bibliography: 101-113 l.
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Functional analysis of novel ��-catenin mutantsAl-Fageeh, Mohamed B. 12 February 2003 (has links)
��-catenin is a multi functional protein that is involved in cell-cell adhesion
and cell signaling. In non-stimulated cells, ��-catenin is tightly down-regulated by
GSK-3��-dependent phosphorylation at Ser and Thr residues, followed by rapid
ubiquitination and proteasomal degradation. It is well established that mutations
within the regulatory GSK-3�� region lead to stabilized ��-catenin and constitutive
��-catenin/TCF-dependent gene activation. Furthermore, it has been shown that
amino acids adjacent to codon 33, namely 32 and 34 of ��-catenin, are hotspots for
substitution mutations in carcinogen-induced animal tumors. Thus, a major
hypothesis of this thesis was that substitution mutations at codon 32 of ��-catenin
interfere with phosphorylation and ubiquitination of ��-catenin.
Site-directed mutagenesis was used to create defined ��-catenin mutants,
namely D32G, D32N, and D32Y. The signaling potential of various ��-catenin was
analyzed in a gene reporter assay by co-transfection with a hTcf cDNA with a
reporter plasmid containing a Tcf-dependent promoter (TOPFlash). There was a
significant enhancement of the reporter gene activity with all ��-catenin mutants
compared to WT ��-catenin after 48 hours of transfection. Protein analysis by
Western blotting showed massive accumulation of mutant ��-catenin. Antibody
specific for phosphorylated ��-catenin showed that the accumulated D32G and
D32N ��-catenin proteins were strongly phosphorylated both in vivo and in vitro,
whereas D32Y ��-catenin exhibited significantly attenuated phosphorylation in vivo.
Further studies showed, however, that none of the mutants was sufficiently
ubiquitinated. In addition, inhibition of the proteasome activity by ALLN was
associated with accumulation of cytosolic ��-catenin, which was transcriptionally
inactive. This suppression of ��-catenin transcriptional capacity was independent of
ALLN-associated apoptosis in the transfected cells. Furthermore, exogenous ��-catenin mediated modest cell survival and rendered cells sensitive to apoptotic stimuli.
Thus, although codon 32 of ��-catenin is not a direct target for
phosphorylation, results from this thesis suggested that it affects the
phosphorylation and ubiquitination of the adjacent Ser-33 residue of ��-catenin,
which is a direct target of GSK-3��. In addition, these results showed for the first
time that the phosphorylation step of ��-catenin is not enough to regulate
transcriptional activity, and that ��-catenin still needs to be ubiquitinated for
successful down-regulation. / Graduation date: 2003
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Mutabiliteit en variabiliteit ...Schouten, Albert Reinard. January 1908 (has links)
Proefschrift--Amsterdam.
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The preservation of duplicate genes by complementary, degenerative mutations and the origin of organismal complexity /Force, Allan Gwynne, January 2000 (has links)
Thesis (Ph. D.)--University of Oregon, 2000. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 150-160). Also available for download via the World Wide Web; free to University of Oregon users.
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The role of the micronucleus in the development of the oral apparatus of paramecium /Chau, Miu-fun. January 1987 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1988.
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Polyploid origins, experimental evolution of gene duplicates, and duplication and divergence of reproductive genesHolloway, Alisha Kay 28 August 2008 (has links)
Not available / text
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Analysis of mutations in the kinesin motor that decouple ATPase activity and microtubule interactionAuerbach, Scott David 28 August 2008 (has links)
Not available / text
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ULTRAVIOLET-INDUCED MUTATION IN BACTERIOPHAGE T4Yarosh, Daniel Bruce January 1978 (has links)
No description available.
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Genome-scale strategies controlling the impact of deleterious mutationsMartincorena, Iñigo January 2012 (has links)
No description available.
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