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Gene therapy, although a young field, has become an intense area of study for the potential treatment of many inborn or acquired diseases, including cancer. The systemic or intratumoural delivery of cytokines and other immune system modulators has shown that protective, specific immunity to tumours is possible and effective. Adenovirus is an extremely useful vehicle for the transfer of genes, due to the ease of its preparation, its ability to be grown to high concentrations, and its capacity to infect a wide variety of cell types, including nonproliferating cells. Although a great deal of research has been done, there are a number of possible improvements, specifically, the consistency and range of transgene expression. The HCMV IE promoter/enhancer, used in a wide range of vector systems, has proven to drive lower levels of transgene expression in murine cells as compared to human cells. The use of the MCMV IE promoter/enhancer within the context of an adenovirus vector system has been investigated to see if more consistent transgene expression is possible in murine models. Studies with a luciferase reporter gene have demonstrated that in all cell types, the MCMV IE promoter/enhancer drives transgene expression to equivalent or higher levels than the HCMV IE promoter/enhancer. Our lab has investigated adenovirus as an intrtumoural gene delivery vehicle for various cytokines, including IL-12. However, the current IL-12-expressing vector carries the IL-12p35 subunit in E1 and the IL-12p40 subunit in E3. A homologous recombination event between E1 sequences in the viral genome and those that are integrated into 293 cells, would produce a replication-competent adenovirus expressing high levels of the p40 subunit, which is a potential antagonist to IL-12. A bicistronic adenovirus vector using the poliovirus IRES was constructed containing all IL-12 coding sequences in E1 to avoid this potential hazard. This virus expressed between 60 ng to 2.5 μg of IL-12/10⁶ cells, depending on the cell type. These levels of expression, however are 7-30-fold lower than those driven by previous IL-12 vector. Although this new virus is not appropriate for tumour immunotherapy, the shuttle plasmids developed during its construction have many potential uses. / Thesis / Master of Science (MS)

International Telemetering Conference Proceedings / October 25-28, 1993 / Riviera Hotel and Convention Center, Las Vegas, Nevada / The increasing availability of powerful yet relatively inexpensive data acquisition and processing techniques has precipitated a radical reappraisal of the methods used to capture, manipulate and store data of all kinds. Some of the recently introduced recording systems can be used both for fast data capture and for high capacity archival/back-up applications - effectively bridging a long-standing divide between these two formerly diverse aspects of data recording and processing. This paper offers a brief overview of a new technology known as micro-track recording, and suggests ways in which system designers and integrators may take full advantage of its important new facilities and features.

International Telemetering Conference Proceedings / October 26-29, 1992 / Town and Country Hotel and Convention Center, San Diego, California / The world of instrumentation data recording has traditionally been concerned with recorder performance in terms of bandwidth, data rate, tape speed and recording time, with the apparently unceasing trend to record more and more data. However, while this may remain a valid perspective for data acquisition, the increasing requirement to integrate equipment into computer based environments has resulted in the need for greater emphasis to be applied to such parameters as data control and interfacing when specifying digital data recording systems. This paper addresses these operational issues and describes the practical implementation of a computer friendly digital cassette recorder which provides a common platform for both high rate data acquisition and computer based data analysis.

International Telemetering Conference Proceedings / October 30-November 02, 1989 / Town & Country Hotel & Convention Center, San Diego, California / In order to satisfy future instrumentation data recording requirements, the availability of high data rate recorders with long record times is most desirable. Also, assurance of commonality amongst the many users of these data requires that any system designed be compatible across the user data base. Hence, the American National Standards Institute (ANSI) has developed a tape format standard that will assure commonality and exchange of data in an acceptable manner. This standard also establishes data rate recording requirements using a commercially available media in cassette form that will satisfy the operational need. DATATAPE is developing a recorder system that will meet the ANSI requirements of the data format on tape, as well as the data rate and record times implied by the standard. This paper discusses the development of the tape format standard and the design of the system that will fully comply with the standard. Test data will be presented to verify system capabilities. Additionally, DATATAPE's progress in developing a system that will comply with the similar Department of Defense (DOD) tape format Standard, MIL-STD- 2179, will be presented, along with a summary of DOD programs that require MIL-STD-2179 compliant recorder systems.

International Telemetering Conference Proceedings / October 30-November 02, 1989 / Town & Country Hotel & Convention Center, San Diego, California / In order to satisfy future instrumentation data recording requirements, the availability of high data rate recorders with long record times is most desirable. Also, assurance of commonality amongst the many users of these data requires that any system designed be compatible across the user data base. Hence, the American National Standards Institute (ANSI) has developed a tape format standard that will assure commonality and exchange of data in an acceptable manner. This standard also establishes data rate recording requirements using a commercially available media in cassette form that will satisfy the operational need. DATATAPE is developing a recorder system that will meet the ANSI requirements of the data format on tape, as well as the data rate and record times implied by the standard. This paper discusses the development of the tape format standard and the design of the system that will fully comply with the standard. Test data will be presented to verify system capabilities. Additionally, DATATAPE’s progress in developing a system that will comply with the similar Department of Defense (DOD) tape format Standard, MIL-STD2179, will be presented, along with a summary of DOD programs that require MIL-STD-2179 compliant recorder systems.

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Diss. (sammanfattning) Uppsala : Universitet, 2005. / Härtill 4 uppsatser.