A dissertation submitted to the Faculty of Medicine University of the Witwatersrand, Johannesburg for the degree of Doctor of Philosophy in Medicine Johannesburg, 1988 / The purpose of this thesis was to investigate the value in clinical situations of the well known in vitro anti-adrenergic effects of magnesium. Research interest in magnesium has been growing rapidly over the last twenty years. However, most of this interest has focused on the clinical consequences of magnesium deficiency states and little attention has been given to magnesium as a therapeutic agent. Despite the wide use of infusions of magnesium sulphate in obstetric practice, there is a dearth of knowledge regarding the actions of magnesium on a cardiovascular system. In laboratory studies, magnesium was shown to inhibit the release of catecholamines from adrenergic nerve terminals, an action which might have great clinical implications. / IT2018

Wong Kwok Kui Wister. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 220-236). / ABSTRACT --- p.1 / OBJECTIVES FOR PROJECT --- p.4 / ABBREVIATIONS --- p.6 / Chapter CHAPTER 1 --- INTRODUCTORY LITERATURE REVIEWS / Chapter 1.1 --- Noradrenaline and adrenaline / Chapter 1.1.1 --- History --- p.8 / Chapter 1.2 --- The adrenal medulla / Chapter 1.2.1 --- The anatomy and microcirculation of the adrenal medulla --- p.10 / Chapter 1.2.2 --- Neuro-endocrine control of adrenal medullary secretion --- p.12 / Chapter 1.3 --- Dopamine / Chapter 1.3.1 --- Introduction --- p.14 / Chapter 1.3.2 --- Levels of DA in plasma and urine --- p.14 / Chapter 1.3.3 --- Origins of DA in the urine --- p.15 / Chapter 1.3.4 --- Control of renal DA production --- p.16 / Chapter 1.3.5 --- The actions of DA in the body --- p.17 / Chapter 1.3.6 --- The DA hypothesis --- p.17 / Chapter 1.3.7 --- Urinary NA and AD --- p.18 / Chapter 1.4 --- ACE inhibitors / Chapter 1.4.1 --- Brief review of the pharmacology of ACE inhibitors --- p.19 / Chapter 1.5 --- Catecholamine assays / Chapter 1.5.1 --- Introduction --- p.21 / Chapter 1.5.2 --- Chemistry --- p.22 / Chapter 1.5.3 --- Bioassay --- p.23 / Chapter 1.5.4 --- Colorimetry --- p.25 / Chapter 1.5.5 --- Fluorometry --- p.25 / Chapter 1.5.6 --- Radiochemical techniques --- p.27 / Chapter 1.5.7 --- Radioimmunoassay --- p.29 / Chapter 1.5.8 --- Chromatography --- p.30 / Chapter CHAPTER 2 --- METHODS - DEVELOPMENT OF A CATECHOLAMINE ASSAY & STABILITY STUDIES ON CATECHOLAMINES / Chapter 2.1 --- High performance liquid chromatography and electrochemical detection of catecholamines / Chapter 2.1.1 --- Introduction --- p.38 / Chapter 2.1.2 --- Basic equipment --- p.39 / Chapter 2.1.3 --- Stock aqueous CAT standards --- p.40 / Chapter 2.1.4 --- Mobile phase of HPLC-ECD --- p.40 / Chapter 2.1.5 --- Determination of mobile phase composition and flow rate --- p.41 / Chapter 2.1.6 --- Electrochemical detection --- p.43 / Chapter 2.1.7 --- Linearity and lowest detection limit of HPLC-ECD system --- p.49 / Chapter 2.1.8 --- Maintenance of HPLC-ECD system --- p.54 / Chapter 2.1.9 --- Discussion --- p.56 / Chapter 2.2 --- Sample pre-treatment & stability studies - Human urine / Chapter 2.2.1 --- Pre-treatment --- p.58 / Chapter 2.2.2 --- Analytical performance of the assay --- p.63 / Chapter 2.2.3 --- Stability studies --- p.85 / Chapter 2.2.4 --- Discussion --- p.101 / Chapter 2.2.5 --- Conclusions --- p.108 / Chapter 2.3 --- Sample pre-treatment & stability studies - Rat urine / Chapter 2.3.1 --- Pre-treatment --- p.111 / Chapter 2.3.2 --- Analytical performance of the assay --- p.111 / Chapter 2.3.3 --- Stability studies --- p.119 / Chapter 2.3.4 --- Discussion --- p.131 / Chapter 2.3.5 --- Conclusions --- p.133 / Chapter CHAPTER 3 --- "URINARY CREATININE, SODIUM AND POTASSIUM MEASUREMENTS" / Chapter 3.1 --- Introduction --- p.135 / Chapter 3.2 --- Method / Chapter 3.2.1 --- Urinary creatinine measurement --- p.135 / Chapter 3.2.2 --- Urinary sodium and potassium measurement --- p.136 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Urinary creatinine measurement --- p.136 / Chapter 3.3.2 --- Urinary sodium and potassium measurement --- p.136 / Chapter 3.4 --- Discussion / Chapter 3.4.1 --- Urinary creatinine measurement --- p.136 / Chapter 3.4.2 --- Urinary sodium and potassium measurement --- p.137 / Chapter 3.5 --- Statistics --- p.138 / Chapter 3.6 --- Chemicals --- p.139 / Chapter CHAPTER 4 --- STUDIES ON URINARY EXCRETION OF CATECHOLAMINES IN MAN / Chapter 4.1 --- Population study on the relationships between dietary salt intake and urinary catecholamine output / Chapter 4.1.1 --- Introduction --- p.141 / Chapter 4.1.2 --- Method --- p.142 / Chapter 4.1.3 --- Results --- p.143 / Chapter 4.1.4 --- Discussion --- p.148 / Chapter 4.1.5 --- Conclusions --- p.149 / Chapter 4.2 --- The effect of oral salt loading on urinary catecholamine output / Chapter 4.2.1 --- Introduction --- p.149 / Chapter 4.2.2 --- Methods --- p.150 / Chapter 4.2.3 --- Results --- p.152 / Chapter 4.2.4 --- Discussion --- p.159 / Chapter 4.2.5 --- Conclusions --- p.161 / Chapter 4.3 --- The effects of perindopril on urinary catecholamine outputs / Chapter 4.3.1 --- Introduction --- p.162 / Chapter 4.3.2 --- Methods --- p.165 / Chapter 4.3.3 --- Results --- p.166 / Chapter 4.3.4 --- Discussion --- p.189 / Chapter 4.3.5 --- Conclusions --- p.190 / Chapter CHAPTER 5 --- STUDY ON URINARY EXCRETION OF CATECHOLAMINES AFTER ORAL ADMINISTRATION OF CAPTOPRIL TO RATS / Chapter 5.1 --- Introduction --- p.192 / Chapter 5.2 --- Methods --- p.192 / Chapter 5.3 --- Results --- p.193 / Chapter 5.4 --- Discussion --- p.210 / Chapter 5.5 --- Conclusions --- p.212 / Chapter CHAPTER 6 --- CONCLUSIONS --- p.214 / Chapter CHAPTER 7 --- FUTURE PROSPECTS --- p.216 / PUBLICATIONS TO DATE --- p.218 / ACKNOWLEDGEMENTS --- p.219 / REFERENCES --- p.220

published_or_final_version / abstract / toc / Anaesthesiology / Master / Master of Philosophy

Catecholamines have been implicated in the regulation of cardiac hypertrophy. The serial injection of a catecholamine (epinephrine, norepinephrine or isoproterenol) will increase cardiac muscle mass. In a canine model of left ventricular hypertrophy secondary to aortic coarctation, the endogen

v, 244 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 1981

v, 244 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 1981

Thesis (Ph.D.) -- University of Adelaide, Dept. of Physiology, 1981.

Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Thesis (M. Phil.)--University of Hong Kong, 1998. / Includes bibliographical references (leaves 133-160).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.