Made available in DSpace on 2016-01-26T18:55:27Z (GMT). No. of bitstreams: 1 Dissertacao Sandro.pdf: 259492 bytes, checksum: 9e24465090764a22ac75d827ad8af255 (MD5) Previous issue date: 2008-08-27 / The effect of a probiotic on the immune response of cattle immunized with one dose of rabies vaccine was evaluated in this study. The Nelore bovines (N=75) were divided randomly into 5 groups: four groups fed with a diet supplemented with the 2, 3, 4 and 8 g the probiotic (Proenzime®)/bovine/day, groups G2, G3, G4 and G8, respectively, for 60 days. The 5th group was used as control. All the animals were immunized with a single dose of rabies vaccine on the first day of the experiment (day 0). The antibody titers were measured on days 0, 30 and 60 by RFFIT test. The results show the significant interaction was found between the probiotic level and vaccination time. There was also a significant difference among rabies titers in groups Gc, G2, G3, G4 and G8 on both days 30 and 60. In group G4, titers of rabies antibody in cattle decreased significantly on day 60. A significant difference (p<0.05) between the frequency of immunized animals in the control group and the other treatments was found on both days 30 and 60. On day 60, the control group did not differ from G2. However, no significant difference (p<0.05) was found among the treatments which were supplemented with probiotic. In addition, no significant difference (p<0.05) between the frequency of immunized animals on days 30 and 60 was found in control (Gc) or any other treatment (G2, G3, G4 or G8). In conclusion, the probiotic (Proenzime®) increase the humoral immune response of bovine immunized with rabies vaccine. / Foi avaliado o efeito do probiótico sobre a resposta imune de bovinos imunizados com uma dose de vacina anti-rábica. Utilizaram-se bovinos Nelore (N=75) divididos randomicamente em 5 grupos: quatro grupos foram suplementados com 2, 3, 4 e 8 g probiótico (Proenzime®)/bovino/dia, grupos G2, G3, G4 e G8, respectivamente, por 60 dias. O 5º grupo foi controle. Todos os animais foram imunizados com uma dose de vacina anti-rábica no dia zero do experimento. Os títulos de anticorpos anti-rábicos foram mensurados nos dias 0, 30, 60 por meio do teste de RFFIT. Os resultados mostraram interação significativa entre concentração de probiótico e tempo de vacinação. Também teve aumento significativo nos títulos de anticorpos anti-rábicos nos grupos Gc, G2, G3, G4 e G8 nos dias 30 e 60. No grupo G4 os títulos de anticorpos anti-rábicos reduziram significativamente no dia 60. Houve diferença significativa (p<0,05) entre a freqüência de animais imunizados entre os grupos controle e tratados nos dias 30 e 60. No dia 60, o grupo controle não diferiu do G2. Além disso, não houve diferença significativa (p<0,05) na freqüência de animais nos dias 30 e 60, no grupo controle ou nos grupos tratados (G2, G3, G4 e G8). Conclui-se que o probiótico (Proenzime®) aumentou a resposta imune humoral em bovinos imunizados com vacina anti-rábica.

Made available in DSpace on 2016-07-18T17:53:05Z (GMT). No. of bitstreams: 1 Dissertacao Sandro.pdf: 259492 bytes, checksum: 9e24465090764a22ac75d827ad8af255 (MD5) Previous issue date: 2008-08-27 / The effect of a probiotic on the immune response of cattle immunized with one dose of rabies vaccine was evaluated in this study. The Nelore bovines (N=75) were divided randomly into 5 groups: four groups fed with a diet supplemented with the 2, 3, 4 and 8 g the probiotic (Proenzime®)/bovine/day, groups G2, G3, G4 and G8, respectively, for 60 days. The 5th group was used as control. All the animals were immunized with a single dose of rabies vaccine on the first day of the experiment (day 0). The antibody titers were measured on days 0, 30 and 60 by RFFIT test. The results show the significant interaction was found between the probiotic level and vaccination time. There was also a significant difference among rabies titers in groups Gc, G2, G3, G4 and G8 on both days 30 and 60. In group G4, titers of rabies antibody in cattle decreased significantly on day 60. A significant difference (p<0.05) between the frequency of immunized animals in the control group and the other treatments was found on both days 30 and 60. On day 60, the control group did not differ from G2. However, no significant difference (p<0.05) was found among the treatments which were supplemented with probiotic. In addition, no significant difference (p<0.05) between the frequency of immunized animals on days 30 and 60 was found in control (Gc) or any other treatment (G2, G3, G4 or G8). In conclusion, the probiotic (Proenzime®) increase the humoral immune response of bovine immunized with rabies vaccine. / Foi avaliado o efeito do probiótico sobre a resposta imune de bovinos imunizados com uma dose de vacina anti-rábica. Utilizaram-se bovinos Nelore (N=75) divididos randomicamente em 5 grupos: quatro grupos foram suplementados com 2, 3, 4 e 8 g probiótico (Proenzime®)/bovino/dia, grupos G2, G3, G4 e G8, respectivamente, por 60 dias. O 5º grupo foi controle. Todos os animais foram imunizados com uma dose de vacina anti-rábica no dia zero do experimento. Os títulos de anticorpos anti-rábicos foram mensurados nos dias 0, 30, 60 por meio do teste de RFFIT. Os resultados mostraram interação significativa entre concentração de probiótico e tempo de vacinação. Também teve aumento significativo nos títulos de anticorpos anti-rábicos nos grupos Gc, G2, G3, G4 e G8 nos dias 30 e 60. No grupo G4 os títulos de anticorpos anti-rábicos reduziram significativamente no dia 60. Houve diferença significativa (p<0,05) entre a freqüência de animais imunizados entre os grupos controle e tratados nos dias 30 e 60. No dia 60, o grupo controle não diferiu do G2. Além disso, não houve diferença significativa (p<0,05) na freqüência de animais nos dias 30 e 60, no grupo controle ou nos grupos tratados (G2, G3, G4 e G8). Conclui-se que o probiótico (Proenzime®) aumentou a resposta imune humoral em bovinos imunizados com vacina anti-rábica.