Prerigor pressurization of bovine semimembranosus muscle at 103.5 MNm⁻² (15,000 psi) for two min at 35°C significantly decreased (P<0.01) muscle pH, and significantly (P<0.01) increased the rate of glycogen degradation during the first 4 hr postmortem. Glycogen levels of pressurized muscle were significantly (P<0.01) lower than those of the control at 1, 2 and 4 hr postmortem. Glucose concentrations were significantly (P<0.01) higher in the treated muscle than in the control at all sampling periods. Until 4 hr postmortem the level of glucose-6-phosphosphate was higher in pressure-treated muscle than in the control. Lactate content increased rapidly after pressurization (1 hr postmortem), resulting in an immediate drop in pH; however, there was no significant difference between control and treated muscles at 24 hr. Pressurized muscle showed a significant (P<0.01) increase in lactate dehydrogenase activity over that of the control muscle at 1 hr but not at 24 hr. Pressurization accelerated catabolism of both creatine phosphate and adenosine triphosphate which resulted in a higher ATP turnover value than that of the control. R-values (a measure of metabolic rate) were significantly correlated (r=-0.95) with degradation of ATP. CPK activity was significantly higher (P<0.01) in pressurized muscle than in the control at 1 hr postmortem but not at 24 hr. Concentrations of ATP, adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophosphate (IMP), bases and nucleosides (adenosine, adenine, inosine, hypoxanthine) and nicotinamide adenine dinucleotide (NAD) were determined by high pressure liquid chromatography. Pressurization completely depleted the ATP supply and increased the IMP concentration at 1, 4 and 24 hr postmortem. At 24 hr postmortem, control samples had more ADP whereas the AMP concentration was higher in the pressurized samples at 1 hr postmortem but not at 24 hr. Concentrations of bases and nucleoside metabolites and NAD did not differ between treatments. / Graduation date: 1984

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