Diss. (sammanfattning) Uppsala : Uppsala universitet, 2009. / Härtill 3 uppsatser.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2009. / Härtill 3 uppsatser.

No description available.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2008. / Härtill 4 uppsatser.

No description available.

No description available.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2007. / Härtill 3 uppsatser.

Introduktion: Låg serumkoncentration av vitamin D3 är förknippat med mer parodontal sjukdom. Syftet med denna studie var att studera effekten av 1α,25-dihydroxyvitamin D3 (vit D3) på humana PDL-cellers (hPDLC) benbildande egenskaper samt inflammatoriska egenskaper i form av uttryck av cytokiner/kemokiner vid LPS-inducerad inflammation.Material och metod: hPDLC, från fyra, friska individer, stimulerades med vit D3 i 4-48 h. Benmarkörerna osteopontin och osteocalcin och pro-inflammatoriskt cytokin/kemokin-uttryck bestämdes genom kvantitativ real-time PCR (qRT-PCR) och enzymkopplad immunadsorberande analys (ELISA). Cytokin- och kemokinuttryck bestämdes efter stimulering med inflammations-inducerande lipopolysackarid (LPS, från Escherichia coli) i närvaro eller frånvaro av vit D3. Bestämning av alkalisk fosfatas (ALP) aktivitet skedde genom infärgning och inkubering med p-nitrofenylfosfat substratlösning. Resultat: Behandling med 30 ng/ml vit D3 i 24 h hade ingen effekt på PDL-cellers morfologi och antal men ökade mRNA-uttrycket av osteopontin och osteocalcin med ca 70 % respektive 40 %. 48 h behandling ökade ALP-aktiviteten i hPDLC ca 2 gånger. Stimulering med LPS [1 µg/ml] i 4 h ökade mRNA-uttryck av interleukin (IL)-6 och kemokin ligand 1 (CXCL1), denna LPS-inducerade ökning reducerades vid behandling med vit D3 [30ng/ml]. Behandling med vit D3 [3-300 ng/ml] i 24 h reducerade den LPS-inducerade ökningen av IL-6 med ca 50%. Konklusion: Vit D3 stimulerar osteogena egenskaper i hPDLC och minskar även deras uttryck av pro-inflammatoriska cytokinen IL-6 och kemokinen CXCL1, vilka tyder på att vit D3 kan ha en positiv effekt i dubbel bemärkelse genom stimulering av parodontal regenerering och motverkan av inflammation i parodontiet.

Introduction: Immune therapy plays a larger role in cancer treatment these days, but in order to find new therapies and improve already existing ones, more knowledge about the immune system is needed. The peripheral blood contains many different cell types, some extensively studied, some less well-known. By using multicolor flow cytometry, the immune status of an individual can be displayed in relatively short time. Aim: The aim of this study was to develop a multicolor flow cytometry method in order to examine the distribution of 31 cell types, with a focus on immune regulatory cells, in peripheral blood in healthy donors, as well as examine the impact of cryopreservation on the different subsets. Methods: After isolating the mononuclear cells from peripheral blood using Ficoll separation, each sample (n = 19) were analyzed with three flow cytometry panels. The remaining cells were cryopreserved in -190°C and later thawed and analyzed the same way as the fresh samples were. Results: The cell distribution in fresh samples were mostly consistent with other studies. While the percentage of many cell types remained unchanged after thawing, the total percentage of T helper cells and some subsets were decreased in frozen samples, leading to a decrease in total T cells. Furthermore, the percentage of total monocytes were increased and the distribution of monocyte subsets were altered in frozen samples, among others. Conclusion: This study confirms the results of other studies of the human immune system and provides valuable knowledge about the impact of cryopreservation.

<p>This licentiate thesis is written at the multi-disciplinary research school Identity and Pluralism−and also within the subject of Church History−at the Department of Culture and Communication, University of Linkoping, Sweden.</p><p>In the thesis the rise of the Cell Group Church is explained. Further the Cell Group Church is analyzed with help of questions regarding identity and pluralism. The author conceives a basis of identity, an approach to define cell-structured churches. This basis can in turn constitute a premise for continued research. Furthermore adequate criteria to evaluate the movement are put forth.</p><p>The dissertation poses three main questions. How was the Cell Group Church formed? Is it possible to identify the Cell Group Church with help of identity markers? What criteria are appropriate to use for an evaluation of the Cell Group Church?</p><p>The concept of the Cell Group Church was coined by Dr. Ralph W. Neighbour and it is he and his book ‘Where Do We Go From Here?’ which are the eye-catchers in this thesis. In what way might Neighbour’s own personal development have affected the design? The intention is not to make a psychological study of Neighbour’s persona, but to weigh in some decisive events that are found in Neighbour’s own biography and that might have affected the design of the Cell Group Church. But influence also has occurred from the outside. What has happened when the Cell Group Church has met the congregations of reality in Korea, Singapore, The Ivory Coast and Columbia? What has Neighbour modified and what in the Cell Group Church has endured? Within the given frame, 1965-2006, there has been an interaction going on between Neighbour and his personal development on the one side and his encounters with reality on the other. In this tension a process of reform is growing. The author will highlight what is lasting in this process, what stands for continuity, and what means a change of identity. The method is to begin with historically descriptive but devolves into being analytical.</p><p>This thesis contributes to the basic research in the field of the Cell Group Church. The movement of the Cell Church is a part of a forceful global course of events within Christianity. There are historians of religion and sociologists of religion who mean that a new kind of Christianity is forming in our times. The manner in which the Cell Group Church relates to its contemporary period is interesting both from international as well as Swedish conditions. Is the Cell Group Church an alternative that will replace the churches of old? Can the Cell Group Church contribute to the survival of the churches of old?</p>