Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 87-123).

Masayo Kotaka. / "May 1999." / Thesis (Ph.D.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (p. 179-190). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Orientador: Roger William Fernandes Moreira / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-24T03:19:50Z (GMT). No. of bitstreams: 1 Mendes_MarceloBrenoMeneses_D.pdf: 3820602 bytes, checksum: 7c13797c10676481e3ebfd0492c570a9 (MD5) Previous issue date: 2014 / Resumo: O objetivo deste estudo foi avaliar, através da análise de rugosidade superficial, microscopia eletrônica de varredura (MEV), análise de composição através de espectroscopia de raios-X e análise de resistência mecânica, dois diferentes sistemas de fixação interna usados em fixação de osteotomia sagital do ramo mandibular. Foram selecionadas cinco placas retas do sistema 2,0 mm com quatro furos e 20 parafusos de 6 mm de comprimento de dois sistemas de fixação interna, grupo I: Tóride (Tóride Indústria e Comércio Ltda. -Mogi Mirim, São Paulo -Brasil) e grupo II: Medartis (Medartis AG -Basel, Suíça). Para os testes mecânicos, foram selecionadas dez hemimandíbulas de poliuretano, dividindo-as igualmente entre os grupos I e II. Inicialmente, foi realizada análise de rugosidade superficial em todas as placas dos dois sistemas, e os dados foram submetidos à análise de variância e teste de Tukey (p>0,05) para a comparação entre as médias. Em seguida, as mesmas placas foram avaliadas no espectrômetro por meio de difração de raios-X e logo após foram analisadas no MEV. Por último, foi realizado o teste mecânico, no qual as hemimandíbulas de poliuretano foram fixadas e submetidas ao teste de carregamento (1mm/min). Os dados foram analisados quanto à carga de pico (em Newtons) e o deslocamento de pico (em mm) para todos os grupos estudados, e posteriormente submetidos ao teste t. Os grupos testados apresentaram vestígios superficiais dos processos de usinagem, principalmente nas margens dos elos. O grupo II apresentou revestimento com uma camada de nitreto de titânio. A análise da rugosidade revelou diferença entre as regiões de centro e margem da placa para o grupo I; diferente do grupo II, o qual apresentou valor de rugosidade menor e mais uniforme. O teste mecânico revelou que o grupo II apresentou maior carga de pico. Os dados demonstraram que há diferença microestrutural e mecânica entre os grupos testados. / Abstract: The aim of this study was to evaluate, through surface roughness analysis, scanning electron microscopy (SEM), composition analysis by X-ray spectroscopy and analysis of mechanical strength, two different systems used in internal fixation of mandibular sagittal split osteotomy fixation. Five straight 2.0 mm system plates with four holes and 20 screws 6 mm length of two internal fixation systems were selected, group I: Toride (Toride Indústria e Comércio Ltda -Mogi Mirim, São Paulo -Brazil.) and group II: Medartis (Medartis AG -Basel, Switzerland). For mechanical testing, ten polyurethane hemimandibles were used, distributing them equally into groups I and II. Initially, surface roughness analysis was performed on all plates of the two systems, and the data were subjected to analysis of variance and Tukey's test (p> 0.05) for comparison between means. Then, the same plates were evaluated in the spectrometer by means of X-ray diffraction and after this evaluation, they were subjected to SEM. Finally, mechanical testing was performed, in which the polyurethane mandibles were fixed and subjected to the load (1mm/min) test on an Instron testing machine, Model 4411 (Instron Corp., Norwood, MA). Data were analyzed for peak load (in Newtons) and peak displacement (in mm) for all groups, and then subjected to t test. The groups tested showed superficial traces of machining processes, especially on links periphery. Group II had coated with a layer of titanium nitride. The roughness analysis revealed differences among the regions of the center and edge of the plate for group I; group II showed roughness value smaller and more uniform, than group I.The mechanical test showed that group II had higher peak load. The data demonstrated that there are microstructural and mechanical differences between the tested groups. / Doutorado / Cirurgia e Traumatologia Buco-Maxilo-Faciais / Doutor em Clínica Odontológica

<p>Rod Salix pripada porodici Salicaceae i obuhvata oko 450 vrsta &scaron;irom sveta, od kojih u Republici Srbiji raste oko 18. Kora vrbe ispoljava antiinflamatorno, antireumatsko, analgetičko i antipiretičko delovanje sinergističkim efektom njenih glavnih aktivnih supstanci &ndash; glikozida salicina, fenolnih i flavonoidnih jedinjenja. Ciljevi ovog rada bili su ispitivanje uticaja klasične i savremenih metoda ekstrakcije na hemijski sastav i biolo&scaron;ke aktivnosti ekstrakata različitih vrsta vrba, utvrđivanje razlika u sadržaju aktivnih komponenti i biolo&scaron;kom potencijalu između ekstrakata kore i ekstrakata lista iste vrste vrbe, kao i utvrđivanje razlika u sadržaju sekundarnih metabolita i antioksidantnoj aktivnosti kod ekstrakata različitih vrsta vrba. Ispitivanja su uključivala analizu 92 ekstrakta kore i lista osam predstavnika roda Salix, dobijenih različitim metodama (maceracija, ultrazvučna i mikrotalasna ekstrakcija) i pri različitim uslovima ekstrakcije (rastvarač, vreme, veličina čestica). Sadržaj ukupnih fenola i flavonoida, kao i antioksidativna aktivnost određeni su spektrofotometrijski. Hemijska karakterizacija ekstrakata vr&scaron;ena je primenom visokoefikasne tečne hromatografije (HPLC). Metodom in silico molekularnog dokinga ispitan je inhibitorni potencijal sastojaka ekstrakata prema enzimima ciklooksigenaze (COX-1 i COX-2) i acetilholinesterazi (AChE). Savremenim metodama ekstrakcije izolovan je veći broj pojedinačnih komponenata u najvećoj koncentraciji i dobijeni su ekstrakti sa jačim potencijalom neutralizacije hidroksilnih radikala. Klasična metoda maceracije 70% etanolom (v/v) bila je pogodnija za dobijanje ekstrakata sa jačim antioksidativnim potencijalom prema DPPH radikalu kod gotovo svih ispitivanih Salix vrsta. Kod većine Salix vrsta jači antioksidantni potencijal prema DPPH radikalu ispoljili su ekstrakti kore. Kod polovine ispitivanih vrsta ekstrakti lista su inhibisali hidroksilne radikale u većoj meri. Veći sadržaj pojedinačnih jedinjenja u ekstraktima kore ili lista zavisio je od same vrste vrbe. Najjaču antioksidantu aktivnost ispoljila je vrsta S. alba, dok je u pogledu hemijskog sastava najraznovrsnija vrsta bila S. fragilis. In silico analizom metodom molekularnog dokinga utvrđen je jak inhibitorni potencijal flavonoidnih jedinjenja kvercetina, naringenina i epikatehina, kao i hlorogenske kiseline među fenolnim kiselinama prema enzimima COX-1 i COX-2. Epikatehin, salicin i hlorogenska kiselina ispoljili su značajno inhibitorno delovanje na enzim AChE u doking studijama. Dobijeni rezultati ukazuju da pored kore i list vrbe ima veliki potencijal primene kao izvor značajnih fitokomponenata, kao i na mogućnost eksploatacije vrsta vrba koje nisu u komercijalnoj upotrebi kao lekovitih sirovina za izolovanje antioksidanasa i farmakolo&scaron;ki aktivnih supstanci.</p> / <p>The genus Salix belongs to the family Salicaceae and comprises about 450 species worldwide, out of which 18 grow in the Republic of Serbia. Willow bark exhibits anti-inflammatory, anti-rheumatic, analgesic and antipyretic effects through synergistic action of its main ingredients -glycosidesalicin, phenolic and flavonoid compounds. Aims of this paper were to analyse the impact of classical and modern extraction methods on chemical composition and biological activities of extracts of different willow species, determine the differences in the amount of active compounds and biological potential between bark and leaf extracts within the same willow species, as well as between extracts of various species. The analysis included 92 bark and leaf extracts of eight species from the genus Salix, obtained by different extraction methods (maceration, ulrasound and microwave assisted extraction) and conditions (solvent, time, particle size). Total phenolics and flavonoids content, as well as antioxidant activity were determined spectrophotometrically. Chemical characterization was carried out by high performance liquid chromatography (HPLC). Cyclooxygenase (COX-1 and COX-2) and acetylcholine esterase (AChE) inhibitory potential of compounds isolated from the extracts was examined by in silico method of molecular docking. Greater number of individual components in highest concentration, as well as exracts with stronger hydroxyl radical scavenging potential were obtained by modern extraction methods. Classical method of maceration with 70% ethanol (v/v) was more suitable for obtaining extracts with higher DPPH radical scavenging activity in the vast majority of tested species. In half of the analysed species leaf extracts inhibited hydroxyl radicals more than bark. Higher amount of individual compounds in bark or leaf extracts depended on the species itself. S. alba exhibited the strongest antioxidant activity, whereas S. fragilis had the most diverse chemical composition. Strong COX-1 and COX-2 inhibitory potential of flavonoids quercetin, naringenin and epicatechin, as well as chlorogenic acid among phenolic acids was determined by in silico molecular docking analysis. Molecular docking studies also demonstrated the inhibitory activity of epicatechin, salicin and chlorogenic acid toward AChE. The obtained results indicate that not only bark, but willow leaf as well could be used as source of significant phytochemicals and also the possibility of exploitation of willow species that are not commercially used as medicinal raw material for isolation of antioxidants and pharmacologycally active substances.</p>