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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 11 to 12 of 12 (0.141 seconds)Spelling suggestions: "subject:"chondrogenic differentiation"" "subject:"chonderogenic differentiation""
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Příprava a charakterizace buněčných modelů lysosomálních dědičných onemocnění - mukopolysacharidos / Preparation and characterization of cell models of lysosomal hereditary diseases - MucopolysaccharidosesPresová, Gabriela January 2020 (has links)
Mucopolysaccharidoses are a group of diseases that belong to lysosomal storage disorders. A common sign of these monogenic multisystem diseases is a gene mutation leading to a deficiency of the lysosomal enzyme participating in glycosaminoglycan degradation. It results to their accumulation in the tissues and organs, where they cause a progressive damage. There is no efficient treatment available for most mucopolysaccharidoses. Moreover, the research is complicated because of the low prevalence and type of affected tissues. Animal models of these human diseases are used for an evaluation of newly developed therapeutic approaches. However, they also have many limitations due to the different pathogenesis and catabolic pathways of the accumulated substrates between humans and animals. Therefore, animal models are replaced by human cell models. In this thesis, the development of four mucopolysaccharidoses human cell models is reported (MPS IIID, MPS IVA, MPS IVB, MPS VI). Corresponding genes (GNS, GALNS, GLB1, ARSB) were inactivated using CRISPR/Cas9 technology, where plasmids containing specific inserts are delivered to the target human induced pluripotent stem cells (iPSC), using electroporation. Isolated clones, which represent iPSC disease models, were characterized by Sanger sequencing, enzyme...
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Proliferations- und Differenzierungsverhalten humaner Zahnkeimzellen der Pulpa / Proliferation and Differentiation Characteristics of Human Pulp Cells taken from Tooth GermsGümmer, Andrea Mirja 15 November 2011 (has links)
No description available.
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