The response of three Eucalyptus spp. clones (GC550, GU210 and TAG14) to water availability was assessed in terms of growth, plant water status, leaf gas exchange, whole plant hydraulic characteristics (both root and shoot), stem xylem vulnerability. Furthermore, to experimentally assess the influence of hydraulic conductance on leaf physiology and plant growth, specimens of two of the clones were subjected to long-term root chilling. Prior to harvesting data were collected on the diurnal variation in leaf water potential (ΨL), transpiration rate (E), stomatal conductance (gs) and net CO2 assimilation rate (A). Main stem xylem vulnerability was assessed using ultrasonic acoustic emissions (UAE). Vulnerability of the main stem was assessed as the leaf water potential corresponding to the maximum rate of acoustic emissions (ΨL, EPHmax), and as the critical water potential triggering cavitation events, calculated as the mean of the water potentials of data points lying between 5 and 10% of the total accumulated emissions (ΨCAV,cUAE,%). Hydraulic conductance was measured on roots and shoots using the high-pressure flow meter (HPFM). Root data were expressed per unit root dry mass (Kr/trdw) and per unit leaf areas (Kr/LA), shoot data expressed per unit shoot dry mass (Ks/tsdw) and per unit leaf area (Ks/LA), and whole plant conductance was expressed per unit leaf area (KP/LA). Soil-to-leaf hydraulic conductance was also assessed as the inverse of the slope of the relationship between leaf water potential and transpiration rate (the evaporative flux, EF, method). A field study was undertaken on three month old TAGl4 and GU210 plants. Diurnal values of leaf water potential ΨL, E and gs were consistently higher in TAG14 than GU210, but A did not differ among the clones. Main stem xylem vulnerability (ΨCAV, cUAE,%) was higher in TAG14 than GU210. In both clones midday ΨL fell below ΨcAv,cUAE,%, suggesting lack of stomatal control of xylem cavitation. Kr/LA was higher in TAGl4 than GU210, whereas, Ks/LA and Ks/tsdw was higher in GU210 than TAGI4. A greater proportion of hydraulic resistances resided in the roots, particularly in GU210. Kp/LA was higher in TAGl4 than GU210 clone, although the significance was marginal (P=0.089). However, all the physiological measurements, were consistent with the concept of higher hydraulic conductances in TAGl4 leading to lower leaf level water stress. Above ground biomass was higher in TAG14 than GU210, in agreement with this concept, although this clone was more vulnerable than GU210. Material grown for 14 months in 25 l pots clones showed no differences in ΨSoil between the high and low watering supply, indicating that even the 'high' supply was inadequate to prevent water stress. In accordance with this, diurnal values of ΨL, gs, E and A did not differ significantly between treatments and clones. Early stomatal closure was apparent, maintaining ΨL constant during the middle of the day. Stem xylem vulnerability, assessed as both ΨL,EPHrnax and ΨCAV,cUAE,% showed that the main stem of GC550 was more vulnerable than other two clones, and that low watered plants were more resistant to xylem cavitation than those receiving high water. Midday ΨL fell below the vulnerability values assessed by both measures across treatments and clones, suggesting lack of stomatal control preventing stem xylem cavitation. There was no relationship between stem xylem cavitation and the shoot hydraulic conductances. Root pressures did not differ between either treatment or clones. Kr/LA was marginally higher in high watered plants, and Ks/LA and Ks/tsdw were higher in low watered plants, possibly by adjustment of leaf hydraulic architecture, and there were no clonal differences. Kr/LA was much lower than Ks/LA. Kp/LA did not differ between the watering treatment, but there was a clonal effect. Growth in dry mass was higher in high watered than low watered plants, but there were no differences among clones. As KP/LA was not affected by watering treatment there was no relationship between KP/LA and growth in total biomass. In plants grown for 21 months in 85 l pots low water treatment decreased midday ΨL, gs, E and A relative to high watered plants. Interclonal differences occurred at midday. Stem xylem vulnerability assessed as ΨCAV,cUAE,% and as ΨL,EPHrnax show similar trends as in the 14 months saplings, clonal differences being significant in ΨL, EPHmax. There was a 1:1 relationship between minimum leaf water potential and ΨL, EPHmax, suggesting that the water potential developed was limited by stem vulnerability. This implies stomatal control to reduce transpiration rates to prevent extensive cavitation occurring. These plants did not develop positive root pressures, indicating that recovery from xylem cavitations occurred through some other process. Kr/LA was higher in high watered plants than those receiving low water, and clonal differences were observed in Kr/trdw. There was no treatment effect in KS/LA and KS/tsdw, but a clonal effect was apparent. KP/LA was significantly different between treatment, and was reduced by low water in two clones, and increased by this in TAGI4. Reduced water availability reduced biomass production, with a greater effect on roots than shoots, such that low watering reduced root:shoot ratios. There was a weak but significant relationship between whole plant hydraulic conductance and maximum stomatal conductance, and between plant conductance and total biomass produced; these data are consistent with the concept of some hydraulic limitation to growth. Root chilling (achieved through chilling the soil) of two of the clones was used to experimentally manipulate hydraulic conductance to test the hydraulic limitation hypothesis. Short-term root chilling decreased both Kr/LA and KP/LA in both clones, but had marginal effects on leaf gas exchange. With long-term chilling the decrease in Kr/LA was observed only in GU210, with TAGl4 showing some adjustment to the treatment. As the roots constitute the major hydraulic resistance, KP/LA largely reflected those of the roots. Long-term root chilling significantly affected leaf physiological characteristics, despite the lack of effect on hydraulic conductance in TAGI4. Long term chilling decreased the whole plant dry mass, but the effect was smaller in TAGI4, and this clone also showed morphological adjustment, in that root growth was less adversely affected than shoot growth. The data from GU210 support the hydraulic limitation hypothesis; because of the morphological and physiological adjustment to long-term root chilling in TAGI4, the data are unsuitable to directly assess the hypothesis. / Thesis (Ph.D.)-University of Natal, Durban, 2002.

Micropropagation methods are often used to supplement existing clonal programmes for Eucalyptus species. However, genotypic differences among clones require the implementation of clone-specific protocols, an expensive and labour-intensive exercise. Hence, this study aimed at determining high-yielding hybrid-specific rather than clone-specific, micropropagation protocols for E. grandis x nitens (GN), E. grandis x nitens (NH), and E. grandis x urophylla (GU). Different conditions for surface sterilisation, bud-break (3 protocols, 2 media), multiplication (4 media), elongation (2 protocols) and rooting (4 media) were tested. A single successful surface sterilisation approach was possible for all clones of the tested hybrids (0.0-11.8% contamination, 0.0-22.9% necrosis). It involved rinsing nodal explants in a fungicide mixture (lg/l Benlate, 1g/1 boric acid, 0.5ml/1 Bravo, Tween 20) for 15 minutes followed by calcium hypochlorite (10g/l with Tween 20) for three minutes. Results at each culture stage were dependent on genotypes, and results indicated here represent ranges in values among the clones of each hybrid. The highest bud-break values for GN clones (87-90%) and NH clones (17-75%) were achieved on a medium containing MS, 0.1mg/1 biotin, 0.1mg/l calcium pantothenate, 0.04mg/1 NAA, 0.11mg/l BAP and 0.05mg/1 kinetin. In GU clones, bud-break values on this medium (84-97%) were not significantly different to those achieved directly on a multiplication medium (80-91%) (MS, 0.1 mg/l biotin, 0.1 mg/l calcium pantothenate, 0.2mg/l BAP, 0.01mg/1 NAA). Shoot multiplication yields for GN clones (4-13 shoots/bud) and GU clones (2-6 shoots/bud) were achieved on a medium consisting of MS, 0.1mg/1 biotin, 0.1 mg/l calcium pantothenate, 0.2mg/1 BAP and 0.01 mg/l NAA. As genotypic effects were highly significant among NH clones, a single multiplication medium for all clones of this hybrid could not be determined. The best method of elongation for clones of all three hybrids involved culturing shoots on MS, 0.1 mg/l calcium pantothenate, 0.1mg/1 biotin, 0.35mg/1 NAA, 0.1mg/l kinetin and 0.1mg/1 IBA, under photoperiod conditions, rather than total darkness, for 6 weeks. This resulted in 82.3-86.6% elongation and shoot lengths increasing by 22.9-35.2 mm for GN clones, 80.2-82.3 % elongation and an increase in length of 24.7-32.2 mm for NH clones and 70.8-78.1 % elongation, and shoot elongation of 21.6-29.3 mm for GU clones from passage 1-2. For all the above stages, media contained 20/25 g/l sucrose and 3.5g/l Gelrite, and cultures were maintained at 25°C ± 2°C day/ 21°C night with a 16 h light/ 8 h dark photoperiod (PPFD 66µmol/m2/s). In terms of rooting, cultures on different media were initially subjected to a 72 hour period of total darkness at room temperature, then a 16 h light/8 h dark photoperiod (PPFD 37µmol/m2 /s) at 24°C day/ 21°C night for 7 days. This was followed by a 16 h light/ 8 h dark photoperiod (PPFD 66µmol/m2/s) at 25°C ± 2°C day/ 21°C night for 21 days. Tested clones of the three hybrids were all rooted successfully (56-93% rooting in GN clones, 36-76% rooting in NH clones and 46-96% rooting in GU clones) on a medium containing ¼ MS, 0.1 mg/l biotin, 0.1 mg/l calcium pantothenate, 0.1mg/l IBA, 0.22g/1 CaCI2 .2H20, 0.185g/l MgS04.7H2O, 15g/l sucrose and 3.5g/1 Gelrite. Predicted yields from the established protocol are also presented (168-667 plants of E. grandis x nitens (GN), 35- 854 plants of E. grandis x nitens (NH) and 54-349 plants of E. grandis x urophylla from 100 initial nodal explants, depending on the clone). Hence, the established protocols can be used successfully for some of the clones, but the implementation of specific media and methods to obtain high yields may still be necessary for certain clones. / Thesis (M.Sc.)-University of Natal, Durban, 2001.

Hybrid clones of the fast-growing Eucalyptus grandis and cold-tolerant E. nitens (GN clones) have been identified by the South African Forestry Industry as being highly suitable for plantations in cold-dry marginal areas. However, one of the main problems regarding their propagation is the difficulty in rooting of cuttings, both in vitro and ex vitro. The aims of this investigation, therefore, were (1) to develop widely applicable and efficient in vitro rooting system(s) for these commercially important clones, and (2) to assess some physiological characteristics of the roots produced. Adventitious shoots (15-20 mm in length) were obtained (l0 shoots/explant) from axillary buds on Murashige and Skoog's (MS) medium containing 0.01 mg.l-1 NAA, 0.01 mg.l-1 IBA and 0.2 g.l-1 FAP. The effect of various medium components, as well as modification of culture environment on in vitro rooting, were investigated. The highest rooting frequencies in clones GN121 (75%) and GN107 (65%) were achieved on l/4 MS with additional 0.22 g.l-1 CaCl2..2H2O and 0.18 g.1-1 MgS04.7H2O, 0.1 mg.l-1 IBA, 0.1 mg.l-1 biotin, 0.1 mg.l-1 calcium pantothenate, 15 g.1-1 sucrose and 4 g.l-1 Gelrite. Best culture conditions were an initial 72-hours dark incubation followed by a 16-hours day/8-hours night photoperiod at a PPFD of 37 µmol.m-2.s-1 and 23°C day/21°C night for seven days, after which the PPFD was increased to 66 µmol.m-2.s-1 at 27°C day/21°C night for 18 days. Towards the development of a more widely applicable in vitro rooting protocol for GN clones, the use of Agrobacterium rhizogenes strains was investigated. Production of transgenic roots was observed on carrot discs and shoots from seedlings of Eucalyptus grandis and E. nitens, but not on shoots of GN clones. Therefore, a method needs to be established for the successful transfer and integration of the Ri plasmid of Agrobacterium into the hybrid plant genome for induction of transgenic roots. The quality of roots produced in vitro and from cuttings was assessed by examination of root anatomy and hydraulic characteristics. Adventitious roots were prepared for measurement of hydraulic conductivity by detopping explants, then filtered, acidified distilled water was drawn through undisturbed potted root systems under partial vacuum, causing no damage to the roots. Initial studies showed that tissue culture-derived roots exhibited a higher specific root mass hydraulic conductivity than those derived from cuttings (6.46 x 10-6 vs. 3.06 X 10-6 g.kPa-1.s-1.g-1 dry root), probably due to root architecture. Curves relating vulnerability to water potential were constructed and both types of roots showed vulnerability to cavitation at high water potentials. Differences were also observed in staining reactions (safranin and fastgreen) which might suggest differences in presence and level of secondary metabolites in these roots at the juvenile stage. Applications of the developed protocols and future research strategies are discussed. / Thesis (M.Sc.)-University of Natal, Durban, 1999.

In South Africa, Eucalyptus trees are used for many processed wood products (e.g. paper) and in the mining industry. Priorities in Eucalyptus breeding programmes include selection of varieties that are fast growers, insect and disease resistant, have appropriate wood characteristics and can grow in a wide variety of environmental conditions. Cold-tolerant cultivars of E. saligna and of E. grandis have been bred and selected in Australia and South Africa, respectively for use in cold regions of Natal Midlands and North Eastern Cape. However, the production of large numbers of such cultivars for planting out in a commercial scale is being impaired by slow growth rate, low regeneration time and poor rooting ability of cuttings from these trees. Consequently, methods of in vitro propagation of cold-tolerant clones were investigated. Axillary buds were induced and subjected to a variety of multiplication, elongation and rooting media. The optimised protocol for the production of shoots from axillary buds was: bud induction medium comprising of MS supplemented with 20 grl sucrose and 10 grl agar for 1-2 weeks, multiplication medium comprising of MS supplemented with 0.1 mgrl biotin, 0.1 mgrl calcium pantothenate, 0.2 mgrl benzyladenine phosphate, 20 grl sucrose and 3.5 grl Gelrite for 4 weeks, elongation medium for 4-6 weeks comprising of MS medium supplemented with 0.1 mgrl biotin, 0.1 mgrl calcium pantothenate, 0.35 mgr' NAA, 0.1 mgr' kinetin, 0.1 mgrl IBA, 20 grl sucrose and 3.5 gr1 Gelrite. Production of plantlets via somatic embryogenesis was also investigated but hampered because of high rates of contamination as pieces of mature leaves were used as exp1ants. Ongoing breeding programmes are aimed at obtaining hybrids of Eucalyptus that are cold tolerant. The hybrid progeny then need to be screened for cold-tolerance. However, a major problem in the selection of cold-tolerant clones is that diagnosis can only be undertaken by assessing the field performance of the genotypes under various environmental conditions. In this regard, a protocol for 1D gel electrophoresis was developed for Eucalyptus species with the view to use it for the detection of cold-tolerant stress proteins. Leaf material from both non-cold tolerant and cold-tolerant clones was used. Well-resolved gels that focused on the comparison' between protein profiles of cold-susceptible and cold-tolerant clones before and after period of cold stress were obtained. The findings of this study showed that two polypeptides, one in the lower molecular region of 14.3-20.1kD and another of a higher molecular weight in the region of 116.4-170 kD were observed after cold acclimation. These changes in polypeptide profiles were observed in cold-tolerant E. grandis x nitens (GN1) and E. saligna (AS 184, AS 196 and TS 15) but not in a non-cold tolerant species E. grandis (TAG 731). These polypeptides may have an important role in the cellular adaptation to cold temperatures. It is suggested that this method may be used as a diagnostic tool for screening cold tolerance on Eucalyptus cultivars. / Thesis (M.Sc.)-University of Natal, Durban, 1996.

The genus Eucalyptus has been planted extensively throughout the world in tropical and subtropical regions, primarily because of its economic importance and use in wood and pulp production. Due to the growing demands for timber, forestry companies need to increase the productivity of available forest land. The genetic improvement of forest trees through selection and breeding involves a lengthy process of scientifically controlled trials focused on short-term and long-term goals using breeding and production populations. This investigation focused on the use of Best Linear Prediction (BLP) and its application to: (1) the prediction of genetic gains for a breeding population and, (2) the selection of superior individuals for clonal production of E. grandis. A CSIR dataset for a 20-year-old progeny trial involving 90 open-pollinated families was obtained. Four traits, namely, diameter at breast height (DBH), stem form, splitting and density were identified for use in this investigation. Relevant data were extracted and a file termed, Dataset created. Dataset was edited, standardized and corrected for the fixed effect of replication using SAS® procedures. Precise and accurate population parameter estimates are fundamental in determining breeding strategies and thus, heritabilities of each trait and phenotypic correlations between traits in Dataset were estimated using SAS® procedures. DBH was found to have the highest heritability (0.600), followed by density (0.492). The estimated heritability for stem form was 0.401 and splitting had the lowest heritability at 0.214. A high positive phenotypic correlation of 0.83 was estimated between DBH and stem form. The phenotypic correlations between other traits were close to zero. An index provides a weighted score for individuals, which takes all relevant information into account and allows individuals or families to be chosen for breeding and production purposes. Consequently, Best Linear Prediction (BLP) of individual breeding values were calculated using MATGEN® (2003). Thereafter, BLP values were used to determine the rankings of individual trees for 15 different selection indices. In order to determine the effect of selection on the change in the population mean of a trait, the breeding population's response to selection was predicted and compared across three selection strategies, namely: (1) individual selection, (2) single-trait index selection, and (3) multiple-trait index selection. The top 8% of individuals in the breeding population were selected for and the genetic gains were predicted. It was found that the response to selection was greatest when using individual selection. Furthermore, DBH had the best selection response for all three strategies as compared to the other traits under investigation. Fifteen indices, considering different numbers and choice of traits, were compared for commonality among rankings of the top 30 individuals. Two methods, namely, (1) a rank-correlation matrix and (2) a manual assessment, were used. The commonality between indices showed that a simple index, considering two traits (DBH and density) was equally effective (93%) in identifying genetically-superior individuals as the more complex index that considered four traits. Furthermore, it was possible to select for only three traits (DBH, splitting, density) and identify the same top 30 individuals as using the index that considered four traits. The researcher's goal was to find the most desirable individuals in the population to be used for production purposes, such as clonal forestry. Consequently, various selection options, specifying certain trait requirements, were used to select superior individuals for use in production and deployment. The "commercial selection" option was the only option successful in obtaining an individual that met the required criteria for the four traits in the population of 475 individuals. The results suggested that breeders should consider large populations and only a few important traits in order to obtain a greater number of individuals suitable for mass propagation in clonal forestry. In order to further investigate the effect of population size on the number of individuals suitable for clonal forestry, a hypothetical population was generated. This was accomplished using between family and within family standard deviation values obtained from Dataset. The large hypothetical population of 1000 individuals produced twelve individuals suitable for production purposes, as opposed to only one in the real population of 475 individuals. This result further indicates that a larger population provides a greater number of individuals appropriate for use in production and deployment. This investigation successfully addressed the aims by: (1) calculating individual breeding values (BLP) and ranking individuals, (2) predicting the breeding population's response to selection, according to three strategies, for the four traits under investigation, and (3) identifying superior individuals for use in commercial clonal forestry. As the work of tree breeders is aimed at improving the growth and quality of trees by increasing the frequency of desirable genotypes in the population, further research could focus on (1) the effect of different sets of economic weightings on index rankings in a population and (2) the influence that population structure has on the optimal genetic gains obtained. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2017-12-08T11:11:14Z No. of bitstreams: 1 PGEF16MA057.pdf: 1146330 bytes, checksum: 87ce579ee0bdc4ea559098e0a886c2f6 (MD5) / Made available in DSpace on 2017-12-08T11:11:14Z (GMT). No. of bitstreams: 1 PGEF16MA057.pdf: 1146330 bytes, checksum: 87ce579ee0bdc4ea559098e0a886c2f6 (MD5) Previous issue date: 2016-02-29 / PROMOP / The objective was to rescue, preserve and multiply the genetic material of the best species/origins selected Eucalyptus spp., to southern Brazil and select the best clones for propagation in Commercial level. The forest Eucalyptus spp. the study is located in the city of Rio Negrinho, SC. The forest was planted in December 1999. The species of the gender Eucalyptus sp. present in the experimental area are grown from seeds, from different locations. For selection of the best sources, subjects were evaluated for survival, height, diameter at breast height, volume, and features like the trunk tortuosity and fork. For vegetative material rescue experiment was applied the techniques of annealing and semi-annealing nine potential species, where individuals were evaluated number of shoots, number of cutting, survival and rooting. The same was evaluated according to the youthfulness through different heights from the ground for E. benthamii. The potential species for quantitative and qualitative variables, as well as the ranking used to select the best species, three species that stood out were E. dunni, E. benthamii and E. dorrigoensis. Regarding the origins for E. smithii the origin Wilson Promontorj to E.benthamii Australia, as well as E. viminalis and E. deanei the origin Manville SC and E. macarthurii Pieter Maritz. Among the vegetative recovery methods tested, the girdling showed better results due to the issuance of shoots. For the survival and 12 13 rooting of seedlings it is necessary to further study since only E. deanei embedded material obtained using IBA at 3000 mg L-¹. As for the issue of shoots depending on the degree of youthfulness to E. benthamii is recommended to perform the incision in the trees at the height of 20 and 50 cm above the ground, and new studies because not yielded results as the survival and rooting of cuttings / O objetivo do estudo foi resgatar, conservar e multiplicar o material genético das melhores espécies/procedências selecionadas de Eucalyptus spp., para a região norte de Santa Catarina e selecionar os melhores clones para a propagação em nível comercial. O povoamento de Eucalyptus spp. do estudo está localizada no município de Rio Negrinho, SC. O talhão foi plantado em dezembro de 1999. As espécies do gênero Eucalyptussp. presentes na área experimental são oriundas de sementes, provenientes de diversos locais. Para seleção das melhores procedências, os indivíduos foram avaliados quanto à sobrevivência, altura, diâmetro à altura do peito, volume, e características qualitativas como tortuosidade do tronco e bifurcação. Para o experimento de resgate de material vegetativo foramaplicadas as técnicas de anelamento e semianelamento em nove espécies potenciais, onde os indivíduos foram avaliados em termos de número de brotações, número de estacas, sobrevivência e enraizamento. O mesmo foi avaliado em função da juvenilidade através das diferentes alturas em relação ao solo para a E. benthamii. As espécies potencias em relação às variáveis quantitativas e qualitativas, assim como o ranking utilizado para selecionar as melhores espécies, as três espécies que se destacaram foram E. dunni. E. benthamiie E. dorrigoensis. Em relação as procedências para E. smithii a procedência de Wilson Promontorj, para E.benthamii a Austrália, assim como para E. deanei e E. viminalis a procedência Manville SC, e para E. macarthuriiPieter Maritz. Dentre os métodos de resgate vegetativo testados, o anelamento apresentou melhores resultados em função da emissão de brotações. Para a sobrevivência e o enraizamento dos propágulos torna-se necessário novos estudos uma vez que apenas E. deanei obteve 8 9 material enraizado com a utilização de AIB na concentração de 3.000 mg L-¹. Quanto a emissão de brotos em função do grau de juvenilidade para E. benthamii, recomenda-se realizar a incisão nas árvores na altura de 20 e 50 cm acima do solo, e a realização de novos estudos pois não obteve-se resultado quanto a sobrevivência e enraizamento das estacas

Submitted by Erika Demachki (erikademachki@gmail.com) on 2017-03-17T20:10:43Z No. of bitstreams: 2 Tese - Ricardo Neves Guimarães - 2017.pdf: 2522150 bytes, checksum: 0037f07faf0bc8b7b76b5d36d87f7823 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-03-20T13:50:39Z (GMT) No. of bitstreams: 2 Tese - Ricardo Neves Guimarães - 2017.pdf: 2522150 bytes, checksum: 0037f07faf0bc8b7b76b5d36d87f7823 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-03-20T13:50:39Z (GMT). No. of bitstreams: 2 Tese - Ricardo Neves Guimarães - 2017.pdf: 2522150 bytes, checksum: 0037f07faf0bc8b7b76b5d36d87f7823 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-01-31 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Cerrado fruit arouse the growing economic interest in different market niches. Among these the Caryocar brasiliense Camb. It stands out due to its economic and social importance. The growing demand pequi fruit for the gastronomic and agroindustrial use indicates the need for studies to produce seedlings for reforestation of degraded areas, reforestation and possible cash crops, meeting the growing demand for seafood pequizeiro. The objective of this study was the development of an initial protocol for the vegetative propagation of pequizeiro through cuttings. For this four experiments were conducted to evaluate different factors which influence the rooting of pequizeiro as leafiness levels, age of the plant, pruning, cutting types and substrates. Through the studies it was found that young plant cuttings have greater potential for cutting with respect to adult trees. The leaves are key to cutting pequi because stakes with six leaflets showed 22.5% while rooting cuttings without leaves not taken root. Regarding the type of stake herbaceous cuttings showed higher callus induction and formation of root primordia in relation to softwood cuttings. But several factors influence the rooting of pequizeiro, further studies should be performed to define a protocol for cutting the pequizeiro. Por Through this study it was found that the pequizeiro introduced himself as a native species of the Cerrado with potential for cutting. / As frutíferas do Cerrado despertam interesse econômico crescente em diferentes nichos de mercado. Entre essas o pequizeiro (Caryocar brasiliense Camb.) se destaca em decorrência de sua importância econômica e social. A demanda crescente dos frutos de pequi para o uso gastronômico e agroindustrial aponta a necessidade de estudos para produção de mudas para revegetação de áreas degradadas, reflorestamentos e possíveis cultivos comerciais, atendendo a crescente demanda pelos frutos do pequizeiro. Objetivou-se com este estudo o desenvolvimento de um protocolo inicial para propagação vegetativa do pequizeiro via estaquia. Para isto foram realizados quatro ensaios avaliando diferentes fatores que influenciam no enraizamento do pequizeiro, como níveis de enfolhamento, idade da planta, podas, tipos de estacas e substratos. Por meio dos estudos verificou-se que estacas de plantas jovens possuem maior potencial para estaquia em relação às árvores adulta. As folhas são fundamentais para estaquia do pequi, pois estacas com seis folíolos apresentaram 22,5% de enraizamento enquanto estacas sem folhas não enraizaram. Quanto ao tipo das estacas herbáceas proporcionaram maior calogênese e formação de primórdios radiculares em relação a estacas semilenhosas. Porém vários fatores influenciam no enraizamento do pequizeiro, novos estudos devem ser realizados para definição de um protocolo para estaquia do pequizeiro. Por meio deste estudo verificou-se que o pequizeiro apresentou-se como uma espécie nativa do Cerrado com potencial para estaquia.