Orientadores: Regina Lúcia de Oliveira Moraes, Eliane Martins / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Tecnologia / Made available in DSpace on 2018-08-19T14:09:37Z (GMT). No. of bitstreams: 1 Fadel_AlineCristine_M.pdf: 3259764 bytes, checksum: a287ca33254d027f23e2f2f818464ee1 (MD5) Previous issue date: 2011 / Resumo: Esse trabalho apresenta os detalhes e os resultados de testes automatizados e manuais que utilizaram a técnica de injeção de falhas e que foram aplicados em redes ópticas. No primeiro experimento o teste foi automatizado e utilizou a emulação de falhas físicas baseadas na máquina de estados do software embarcado dessa rede. Para esse teste foi utilizado uma chave óptica que é controlada por um robô de testes. O segundo experimento foi um teste manual, que injetou falhas nas mensagens de comunicação do protocolo dessa rede, a fim de validar os mecanismos de tolerância a falhas do software central dessa rede. Esse experimento utilizou a metodologia Conformance and Fault injection para preparar, executar e relatar os resultados dos casos de testes. Nos dois experimentos também foi utilizado um padrão de documentação de testes que visa facilitar a reprodução dos testes, a fim de que eles possam ser aplicados em outros ambientes. Com a aplicação desses testes, a rede óptica pode alcançar uma maior confiabilidade, disponibilidade e robustez, que são características essenciais para sistemas que requerem alta dependabilidade / Abstract: This work presents the details and the results of automatic and manual tests that used the fault injection technique and were applied on GPON network. In the first experiment the test was automated, and it performed the emulation of physical faults based on the state machine of the embedded software in this network. In this test is used an optical switch that is controlled by a test robot. The second experiment was a manual test, which injected faults on protocol communication message exchanged through the optical network, in order to validate the main software fault tolerance mechanisms. This experiment used a Conformance and Fault injection methodology to prepare, execute and report the results of the test cases. In both experiments, it was used a standard test documentation to facilitate the reproduction of the tests, so that they can be applied in other environments. With applying both tests, the optical networks reach greater reliability, availability and robustness. These attributes are essential for systems that require high dependability / Mestrado / Tecnologia e Inovação / Mestre em Tecnologia

Signaling pathways control the fate of the cell. For example, they promote cell survival or commit the cell to death (apoptosis) in response to cell injury or developmental stimuli, decisions, which are vital for the proper development and functioning of metazoan. Tight control of such pathways is essential; dysregulation of apoptosis can disrupt the delicate balance between cell proliferation and cell death ending up in pathological processes, including cancer, autoimmunity diseases, inflammatory diseases, or degenerative disorders. We have used a structural genomic approach to study the structure and function of key proteins involved in apoptosis and lipid signaling: the antiapoptotic Bcl-2 family member Bfl-1 in complex with a Bim peptide, the BIR domains of the Inhibitor of Apoptosis (IAP) family members, cIAP2 and NAIP and the a lipid kinase YegS. The structural analysis of the apoptosis regulatory proteins has revealed important information on the structural determinants for recognition of interacting proteins, which can now assist in the development of therapeutic drugs for human diseases. The structural and complementing biochemical studies of the lipid kinase YegS have reveled the first detailed information on a lipid kinase and explained important aspects of its structure-function relationship. Finally, one subject of this work aim to solve what is arguably the most challenging problem in structural projects – to obtain a high production level of proteins suitable for structural studies. We have developed a highthroughput protein solubility screening, the colony filtration (CoFi) blot, which allows soluble clones to be identified from large libraries of protein variants and now constitute a powerful tool for solving difficult protein production problems.