This study investigates coal discard cover soil fertility and its potential for degradation, particularly in terms of its salinisation and acidification potential. Seven rehabilitated coal discard dumps in the Witbank, Ermelo and Newcastle regions were used as study areas. All areas were rehabilitated with a cover soil layer, revegetated and annually fertilised with nitrate fertilisers, super phosphate, kraal manure and lime. Performance guideline for pH of 5.5-(6.5 i0.5)-7.5 and electrical conductivity guideline of preferably less than 200 mS.rn-' but not higher than 400 mS.m-' were set based on literature information. Soil chemical data from a three-year fertilisation programme were used to assess the fertility of the cover soil surface (0-150mm). Data collected over a three year period as well as additional electrical conductivity and pH measurements from the cover soil surface, subsoil, cover soil/coal contact zone and underlying coal itself were used to assess the occurrence of salinisation and acidification of the cover soil. The soil fertility varied significantly among dumps as well as over the three years. Results indicated an increase in ammonium acetate extractable macro elements (calcium, magnesium and potassium). With the exception of manganese, no micro-element toxicities were recorded. Iron concentrations were slightly elevated in some of the sandy cover soil layers. No increase in soluble nitrogen (nitrate and ammonium) was found and most soluble nitrogen was in the form of nitrates. In general the Bray extractable phosphate increased during the study period. It can be predicted that with the following fertiliser programme increases of exchangeable macro-elements as well as available phosphorus can be expected. The study could not indicate an increase in adsorbed or available nitrogen. Organic carbon was initially not analysed therefore no comments can be made whether organic matter increased. Four of the seven dumps surveyed had comparably similar organic carbon levels to the background samples. Overall the fertiliser programme increased the electrical conductivity and decreased the acidity of the cover soil surface. Acidity and salinity was in general not a problem at the surface of the cover soil and pH was even slightly higher in cover soil samples. The acidity and especially salinity increased at the subsoil and so did the sulphate concentrations. Calcium and magnesium sulphate were predominantly responsible for higher electrical conductivity measurements. The percentage exchangeable sodium was also predominantly less than 2% indicating that sodicity is not currently a problem in cover soil. Soil fertility was satisfactory for vegetation growth and macroelement concentrations were in the correct ratio although calcium was slightly high. An elevated sulphate concentration, in comparison to the natural grassland soils, as well as a high salinity and high acidity in the subsoil layers indicate that salinisation and acidification could deteriorate without proper management. A slightly acidic cover soil can also be attributed partially to its natural acidic pH due to the wellweathered and leach property of burrow pit. Higher than recommended salinity levels were found in subsoil samples but the occurrence of acidification of the subsoil was more dump specific. In relation to acidity and salinity guidelines only the cover soil of one dump was concerning and the larger dumps subsoil acidity and salinity were elevated. The following management strategies are proposed: a) The acidification potential, and therefore the pyrite content of the coal discard must be considered during decisions making on the rehabilitation method (clay barriers), topsoil depth, maintenance and mine closure potential. b) The occasional monitoring of the subsoil's and coal contact acidity is recommended, although not much can be done to stop acidification after cover-soil placement. c) To ensure a more sustained from of nitrogen supplementation over the long term the use of selected legumes should be investigated. Research in Europe and Australia suggested that nitrogen fixation could contribute substantially to the nitrogen for plant uptake. d) The physical properties of the topsoil (bulk density 8 soil compaction) are also being neglected and needs to be assessed occasionally and interpreted together with chemical analyses. Observations in other studies indicate that this could be the most fundamental problem for vegetation growth and not necessarily soil fertility, since soil physical properties could have a major impact on root development. Key words: Coal discard, mine rehabilitation, soil fertility, topsoil degradation, salinisation, and acidification / Thesis (M. Environmental Management)--North-West University, Potchefstroom Campus, 2004.

This study investigates coal discard cover soil fertility and its potential for degradation, particularly in terms of its salinisation and acidification potential. Seven rehabilitated coal discard dumps in the Witbank, Ermelo and Newcastle regions were used as study areas. All areas were rehabilitated with a cover soil layer, revegetated and annually fertilised with nitrate fertilisers, super phosphate, kraal manure and lime. Performance guideline for pH of 5.5-(6.5 i0.5)-7.5 and electrical conductivity guideline of preferably less than 200 mS.rn-' but not higher than 400 mS.m-' were set based on literature information. Soil chemical data from a three-year fertilisation programme were used to assess the fertility of the cover soil surface (0-150mm). Data collected over a three year period as well as additional electrical conductivity and pH measurements from the cover soil surface, subsoil, cover soil/coal contact zone and underlying coal itself were used to assess the occurrence of salinisation and acidification of the cover soil. The soil fertility varied significantly among dumps as well as over the three years. Results indicated an increase in ammonium acetate extractable macro elements (calcium, magnesium and potassium). With the exception of manganese, no micro-element toxicities were recorded. Iron concentrations were slightly elevated in some of the sandy cover soil layers. No increase in soluble nitrogen (nitrate and ammonium) was found and most soluble nitrogen was in the form of nitrates. In general the Bray extractable phosphate increased during the study period. It can be predicted that with the following fertiliser programme increases of exchangeable macro-elements as well as available phosphorus can be expected. The study could not indicate an increase in adsorbed or available nitrogen. Organic carbon was initially not analysed therefore no comments can be made whether organic matter increased. Four of the seven dumps surveyed had comparably similar organic carbon levels to the background samples. Overall the fertiliser programme increased the electrical conductivity and decreased the acidity of the cover soil surface. Acidity and salinity was in general not a problem at the surface of the cover soil and pH was even slightly higher in cover soil samples. The acidity and especially salinity increased at the subsoil and so did the sulphate concentrations. Calcium and magnesium sulphate were predominantly responsible for higher electrical conductivity measurements. The percentage exchangeable sodium was also predominantly less than 2% indicating that sodicity is not currently a problem in cover soil. Soil fertility was satisfactory for vegetation growth and macroelement concentrations were in the correct ratio although calcium was slightly high. An elevated sulphate concentration, in comparison to the natural grassland soils, as well as a high salinity and high acidity in the subsoil layers indicate that salinisation and acidification could deteriorate without proper management. A slightly acidic cover soil can also be attributed partially to its natural acidic pH due to the wellweathered and leach property of burrow pit. Higher than recommended salinity levels were found in subsoil samples but the occurrence of acidification of the subsoil was more dump specific. In relation to acidity and salinity guidelines only the cover soil of one dump was concerning and the larger dumps subsoil acidity and salinity were elevated. The following management strategies are proposed: a) The acidification potential, and therefore the pyrite content of the coal discard must be considered during decisions making on the rehabilitation method (clay barriers), topsoil depth, maintenance and mine closure potential. b) The occasional monitoring of the subsoil's and coal contact acidity is recommended, although not much can be done to stop acidification after cover-soil placement. c) To ensure a more sustained from of nitrogen supplementation over the long term the use of selected legumes should be investigated. Research in Europe and Australia suggested that nitrogen fixation could contribute substantially to the nitrogen for plant uptake. d) The physical properties of the topsoil (bulk density 8 soil compaction) are also being neglected and needs to be assessed occasionally and interpreted together with chemical analyses. Observations in other studies indicate that this could be the most fundamental problem for vegetation growth and not necessarily soil fertility, since soil physical properties could have a major impact on root development. Key words: Coal discard, mine rehabilitation, soil fertility, topsoil degradation, salinisation, and acidification / Thesis (M. Environmental Management)--North-West University, Potchefstroom Campus, 2004.

The rehabilitation of degraded soils, such as those associated with post-mining sites, requires knowledge of the soil ecosystem and its physical, chemical, and biological composition in order for rehabilitation efforts to fulfil the long-term goal of reconstructing a stable ecosystem for rehabilitated mine soil. This study addresses the need for appropriate assessment criteria to determine the progress of rehabilitation and subsequently the success of management practices. Significant contributions made by this investigation included the establishment of minimum and maximum values for microbial community measurements from two case studies of rehabilitated coal discard sites. Furthermore, it was shown that there was no relationship between changes in microbial community function and structure and the rehabilitation age of the sites. Following this, the considerable impact of management practices on microbial communities was illustrated. The first part of the study investigated the temporal changes in microbial community function and structure in a chronosequence of rehabilitated coal discard sites aged 1 to 11 years. The most important observation made during the investigation of the microbial communities in the different aged soil covers of the rehabilitated coal discard sites, was that there was no relationship between rehabilitation age and microbial activity or abundance of certain microbial groups. What was responsible for a clear differentiation between sites and a shift in microbial community attributes was the management practices applied. A comparison of two chronosequences of rehabilitated coal discard sites was achieved by an application of the 'space-for-time' hypothesis. Sites of different ages and at separate locations ('space') were identified to obtain a chronosequence of ages ('time'). The two chronosequences included sites aged 1 to 11 years (chronosequence A) and 6 to 17 years (chronosequence B), respectively. Sites in the same chronosequence were managed identically, while there was a distinct difference in management practices applied to each chronosequence. The long-term effect of the different management regimes on the soil microbial community function and structure was investigated. Again, there was no relationship between rehabilitation age and microbial community measurements. Fluctuations of selected microbial properties occurred in both chronosequences and similar temporal trends existed over the rehabilitation periods. However, the less intensively managed chronosequence (8) seemed more stable (less fluctuation occurred) over the rehabilitation period than the more intensively managed chronosequence (A). It was therefore concluded that the microbial communities in the less managed sites maintained their functional and structural integrity within bounds in the absence of management inputs or disturbance. While there was similarity in the trends over time for individual microbial community measurements, the seemingly more stable conditions in chronosequence 6 are important in terms of the goal of rehabilitation. / Thesis (Ph.D. (Environmental Science)--North-West University, Potchefstroom Campus, 2007

Thesis (Ph.D. (Environmental Science)--North-West University, Potchefstroom Campus, 2007.

Mining activities cause severe disturbance to the soil environment in terms of soil quality and productivity and are of serious concern worldwide. Under South African legislation, developers are required to ecologically rehabilitate damaged environments. The application of agronomic approaches for the rehabilitation of coal discard sites has failed dismally in the arid areas of southern Africa. It is obvious that compliance with mitigation and rehabilitation requirements cannot be enforced without a thorough understanding of the ecological principles that ensure ecological stability and subsequent sustainability of soil ecosystems. Soil micro organisms are crucial role-players in the processes that make energy and nutrients available for recycling in the soil ecosystem. Poor management practices and other negative impacts on soil ecosystems affect both the physical and chemical properties of soil, as well as the functional and structural properties of soil microbial communities. Disturbances of soil ecosystems that impact on the normal functioning of microbial communities are potentially detrimental to soil formation, energy transfers, nutrient cycling, plant reestablishment and long-term stability. In this regard, an extensive overview of soil properties and processes indicated that the use of microbiological and biochemical soil properties, such as microbial biomass, enzymatic activity and the analysis of microbial community structure by the quantification of specific signature lipid biomarkers are useful as indicators of soil ecological stress or restoration properties because they are more responsive to small changes than physical and chemical characteristics. In this study, the relationship between the physical and chemical characteristics and different biological indicators of soil quality in the topsoil covers of seven coal discard sites under rehabilitation in South Africa, as well as three reference sites was investigated. Through the assimilation of basic quantitative data and the assessment of certain physical, chemical and biological properties of the topsoil covers obtained from the various coal discard sites as well as the reference sites, the relative success or progress of rehabilitation and the possible correlation between the biological indicators of soil quality and the establishment of self sustaining vegetation covers was determined. Results from soil physical and chemical analyses and percentage vegetation cover were correlated with the results obtained for the functional and structural diversity of microbial communities at the various sites. All results were investigated through statistical and multivariate analysis and the most prominent physical and chemical parameters that influence the biological and biochemical properties of the soil and possibly the establishment of self-sustainable vegetation cover on these mine-tailing sites were identified. Results obtained from this study indicated no significant difference (p>0.05) between the various discard sites based on conventional microbiological enumeration techniques. However, significant differences (p<0.05) could be observed between the three reference sites. All enzymatic activities assayed for the rehabilitation sites, with the exception of urease and alkaline phosphatase displayed a strong, positive association with the organic carbon content (%C). Ammonium concentration had a weak association with all the enzymes studied and pH only showed a negative association with acid phosphatase activity. A positive association was observed between the viable microbial biomass, vegetation cover and the organic carbon content, ammonium, nitrate and phosphorus concentrations of the soil. The various rehabilitation and reference sites could be differentiated based on the microbial community structure as determined by phospholipid fatty acid (PLFA) analysis. It is hypothesised that the microbial community structure of the Hendrina site is not sustainable when classified along an r-K gradient and that the high percentage of vegetation cover and high levels of estimated viable microbial biomass are an artificial reflection of the current management practices being employed at this site. Results obtained during this study, suggest that an absence or low percentage of vegetation cover and associated lower organic matter content of the soil have a significant negative impact on soil biochemical properties (enzymatic activity) as well as microbial population size. Furthermore, prevailing environmental physico-chemical and management characteristics significantly influences the vegetation cover and subsequently the microbial community structure. The results indicate that the microbial ecosystems in the coal discard sites could become more stable and ecologically self-regulating, provided effective management to enhance the organic carbon content of the soil. This could enhance nutrient cycling, resulting in changes of soil structure and eventually an improved soil quality which could facilitate the establishment of self sustaining vegetation cover. Results obtained during this study suggest that a polyphasic assessment of physical and chemical properties; microbial activities by enzymatic analysis; the characterisation of microbial community structure by analysis of phospholipid fatty acids; and the multifactorial analysis of the data obtained can be used as complementary assessment criteria for the evaluation of the trend of rehabilitation of mine tailings and discard sites. Strategic management criteria are recommended based on the soil quality environmental sustainability indices to facilitate the establishment of self sustainable vegetation covers. The contribution of this research to soil ecology is significant with regards to the intensive investigation and explanation of characteristics and processes that drive ecological rehabilitation and determine the quality of the soil environment. The multidisciplinary approach that is proposed could, furthermore, assist in the successful rehabilitation and establishment of self-sustaining vegetation covers at industrially disturbed areas, as well as assist in improving degraded soil quality associated with both intensive and informal agriculture. Additionally, this approach could negate the negative social and environmental impacts frequently associated with these activities. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2004.

Mining activities cause severe disturbance to the soil environment in terms of soil quality and productivity and are of serious concern worldwide. Under South African legislation, developers are required to ecologically rehabilitate damaged environments. The application of agronomic approaches for the rehabilitation of coal discard sites has failed dismally in the arid areas of southern Africa. It is obvious that compliance with mitigation and rehabilitation requirements cannot be enforced without a thorough understanding of the ecological principles that ensure ecological stability and subsequent sustainability of soil ecosystems. Soil micro organisms are crucial role-players in the processes that make energy and nutrients available for recycling in the soil ecosystem. Poor management practices and other negative impacts on soil ecosystems affect both the physical and chemical properties of soil, as well as the functional and structural properties of soil microbial communities. Disturbances of soil ecosystems that impact on the normal functioning of microbial communities are potentially detrimental to soil formation, energy transfers, nutrient cycling, plant reestablishment and long-term stability. In this regard, an extensive overview of soil properties and processes indicated that the use of microbiological and biochemical soil properties, such as microbial biomass, enzymatic activity and the analysis of microbial community structure by the quantification of specific signature lipid biomarkers are useful as indicators of soil ecological stress or restoration properties because they are more responsive to small changes than physical and chemical characteristics. In this study, the relationship between the physical and chemical characteristics and different biological indicators of soil quality in the topsoil covers of seven coal discard sites under rehabilitation in South Africa, as well as three reference sites was investigated. Through the assimilation of basic quantitative data and the assessment of certain physical, chemical and biological properties of the topsoil covers obtained from the various coal discard sites as well as the reference sites, the relative success or progress of rehabilitation and the possible correlation between the biological indicators of soil quality and the establishment of self sustaining vegetation covers was determined. Results from soil physical and chemical analyses and percentage vegetation cover were correlated with the results obtained for the functional and structural diversity of microbial communities at the various sites. All results were investigated through statistical and multivariate analysis and the most prominent physical and chemical parameters that influence the biological and biochemical properties of the soil and possibly the establishment of self-sustainable vegetation cover on these mine-tailing sites were identified. Results obtained from this study indicated no significant difference (p>0.05) between the various discard sites based on conventional microbiological enumeration techniques. However, significant differences (p<0.05) could be observed between the three reference sites. All enzymatic activities assayed for the rehabilitation sites, with the exception of urease and alkaline phosphatase displayed a strong, positive association with the organic carbon content (%C). Ammonium concentration had a weak association with all the enzymes studied and pH only showed a negative association with acid phosphatase activity. A positive association was observed between the viable microbial biomass, vegetation cover and the organic carbon content, ammonium, nitrate and phosphorus concentrations of the soil. The various rehabilitation and reference sites could be differentiated based on the microbial community structure as determined by phospholipid fatty acid (PLFA) analysis. It is hypothesised that the microbial community structure of the Hendrina site is not sustainable when classified along an r-K gradient and that the high percentage of vegetation cover and high levels of estimated viable microbial biomass are an artificial reflection of the current management practices being employed at this site. Results obtained during this study, suggest that an absence or low percentage of vegetation cover and associated lower organic matter content of the soil have a significant negative impact on soil biochemical properties (enzymatic activity) as well as microbial population size. Furthermore, prevailing environmental physico-chemical and management characteristics significantly influences the vegetation cover and subsequently the microbial community structure. The results indicate that the microbial ecosystems in the coal discard sites could become more stable and ecologically self-regulating, provided effective management to enhance the organic carbon content of the soil. This could enhance nutrient cycling, resulting in changes of soil structure and eventually an improved soil quality which could facilitate the establishment of self sustaining vegetation cover. Results obtained during this study suggest that a polyphasic assessment of physical and chemical properties; microbial activities by enzymatic analysis; the characterisation of microbial community structure by analysis of phospholipid fatty acids; and the multifactorial analysis of the data obtained can be used as complementary assessment criteria for the evaluation of the trend of rehabilitation of mine tailings and discard sites. Strategic management criteria are recommended based on the soil quality environmental sustainability indices to facilitate the establishment of self sustainable vegetation covers. The contribution of this research to soil ecology is significant with regards to the intensive investigation and explanation of characteristics and processes that drive ecological rehabilitation and determine the quality of the soil environment. The multidisciplinary approach that is proposed could, furthermore, assist in the successful rehabilitation and establishment of self-sustaining vegetation covers at industrially disturbed areas, as well as assist in improving degraded soil quality associated with both intensive and informal agriculture. Additionally, this approach could negate the negative social and environmental impacts frequently associated with these activities. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2004.

The rehabilitation of degraded soils, such as those associated with post-mining sites, requires knowledge of the soil ecosystem and its physical, chemical, and biological composition in order for rehabilitation efforts to fulfil the long-term goal of reconstructing a stable ecosystem for rehabilitated mine soil. This study addresses the need for appropriate assessment criteria to determine the progress of rehabilitation and subsequently the success of management practices. Significant contributions made by this investigation included the establishment of minimum and maximum values for microbial community measurements from two case studies of rehabilitated coal discard sites. Furthermore, it was shown that there was no relationship between changes in microbial community function and structure and the rehabilitation age of the sites. Following this, the considerable impact of management practices on microbial communities was illustrated. The first part of the study investigated the temporal changes in microbial community function and structure in a chronosequence of rehabilitated coal discard sites aged 1 to 11 years. The most important observation made during the investigation of the microbial communities in the different aged soil covers of the rehabilitated coal discard sites, was that there was no relationship between rehabilitation age and microbial activity or abundance of certain microbial groups. What was responsible for a clear differentiation between sites and a shift in microbial community attributes was the management practices applied. A comparison of two chronosequences of rehabilitated coal discard sites was achieved by an application of the 'space-for-time' hypothesis. Sites of different ages and at separate locations ('space') were identified to obtain a chronosequence of ages ('time'). The two chronosequences included sites aged 1 to 11 years (chronosequence A) and 6 to 17 years (chronosequence B), respectively. Sites in the same chronosequence were managed identically, while there was a distinct difference in management practices applied to each chronosequence. The long-term effect of the different management regimes on the soil microbial community function and structure was investigated. Again, there was no relationship between rehabilitation age and microbial community measurements. Fluctuations of selected microbial properties occurred in both chronosequences and similar temporal trends existed over the rehabilitation periods. However, the less intensively managed chronosequence (8) seemed more stable (less fluctuation occurred) over the rehabilitation period than the more intensively managed chronosequence (A). It was therefore concluded that the microbial communities in the less managed sites maintained their functional and structural integrity within bounds in the absence of management inputs or disturbance. While there was similarity in the trends over time for individual microbial community measurements, the seemingly more stable conditions in chronosequence 6 are important in terms of the goal of rehabilitation. / Thesis (Ph.D. (Environmental Science)--North-West University, Potchefstroom Campus, 2007