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Estudo Comparativo das t?cnicas de Imunofluoresc?ncia Direta, Imunofluoresc?ncia Indireta e Colora??o de Sellers em amostra de tecido nervoso de c?es e de animais silvestres para o diagn?stico da cinomose. / Comparative study of the techniques of Direct Immunofluorescence, Indirect Immunofluorescence and Stain Sellers in samples of nervous tissue of dogs and wild animals for diagnostic of the canine distemperBarreto, Michelle Gomes 27 July 2006 (has links)
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Previous issue date: 2006-07-27 / It was performed a study comparative in the diagnostic of the canine distemper through
techniques of Direct Immunofluorescence (IFD), Indirect Immunofluorescence (IFI) and
Stain Sellers in samples of nervous tissue of dogs and wild animals that died with suspicion
clinical of the viruse. Were utilized 118 samples of central nervous system (SNC) of
domestic dogs and 3 wild animals, 2 skunks and 1 monkey, utilizing Ammon?s horn (CA),
cerebellum (CB) and brain stem (TE). The TE was the region of larger percentage of
positive, following of CA. The comparation between Stain Sellers and IFD, this last
demonstrated a large sensitive. The IFI observed what to the realization of the technique
was necessary the utilization of fresh materials. The utilization of the 117 samples in
conditions of autolysis presented negative result. However 1 sample not autolysate, it was
result positive in the 3 brain regions (CA, CB, TE). It was not possible the comparative
with the technique of IFI, because in the work the technique alone was possible in materials
conserved, with the advantage of a read more easy. In the work was detected also canine
distemper in a skunk through technique of IFD. / Foi realizado um estudo comparativo do diagn?stico da cinomose pelas t?cnicas de
Imunofluoresc?ncia Direta (IFD), Imunofluoresc?ncia Indireta (IFI) e Colora??o de Sellers
em amostras de tecido nervoso de c?es e de animais silvestres que morreram com suspeita
cl?nica da virose. Foram utilizados 118 amostras de Sistema nervoso central (SNC) de c?es
dom?sticos e de 3 animais silvestres , dois gamb?s e um mico, utilizando-se Corno de
Ammon (CA), Cerebelo (CB) e Tronco Encef?lico (TE). O TE foi ? regi?o de maior
porcentagem de positivos, seguido do CA. A compara??o entre a Colora??o de Sellers, e a
IFD, revelou uma maior sensibilidade nesta ?ltima. Na IFI, observamos que para a
realiza??o da t?cnica torna-se necess?rio ? utiliza??o de material fresco. Tendo em vista a
utiliza??o das 117 amostras em estado de aut?lise apresentaram resultados negativos e
apenas 1 n?o autolisado, teve seu resultado positivo nas 3 regi?es cerebrais (CA, CB e TE).
N?o foi poss?vel a compara??o com a t?cnica de IFI, porque nesse trabalho, a t?cnica s? foi
poss?vel em materiais conservados, com a vantagem de uma leitura mais f?cil. Nesse
trabalho foi detectado tamb?m cinomose em um gamb? pela t?cnica de IFD.
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